breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 93,243 A→G T26T (ACA→ACG)  murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 118,308 A→G V113A (GTG→GCG)  nadC ← quinolinate phosphoribosyltransferase (decarboxylating)
RA 123,073 G→A Q19Q (CAG→CAA)  aceE → pyruvate dehydrogenase E1 component
RA 144,694 A→G S40G (AGC→GGC)  yadI → putative PTS enzyme IIA component YadI
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 280,710 (C)5→6 coding (26/351 nt) yagB ← orphan antitoxin YagB
RA 289,587 G→A A240V (GCC→GTC)  argF ← CP4‑6 prophage; ornithine carbamoyltransferase ArgF
RA 295,865 A→G V411A (GTT→GCT)  intF ← CP4‑6 prophage; putative phage integrase
RA 302,374 A→G V67A (GTG→GCG)  paoA ← aldehyde dehydrogenase, Fe‑S subunit
RA 308,054 (C)7→8 coding (1279/2526 nt) ecpC ← putative fimbrial usher protein EcpC
RA 335,624 (A)5→4 coding (345/606 nt) yahD → ankyrin repeat‑containing protein YahD
RA 422,922 T→C R135R (CGT→CGC)  malZ → maltodextrin glucosidase
RA 482,420 T→A M662L (ATG→TTG)  acrB ← multidrug efflux pump RND permease AcrB
RA 506,109 C→T A399V (GCC→GTC)  ushA → 5'‑nucleotidase/UDP‑sugar hydrolase
RA 510,024 C→T A452A (GCG→GCA)  copA ← Cu(+) exporting P‑type ATPase
RA 525,455 A→G D732G (GAC→GGC)  rhsD → protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 533,779 A→G T257A (ACC→GCC)  allR → DNA‑binding transcriptional repressor AllR
RA 685,944 G→A N242N (AAC→AAT)  gltJ ← glutamate/aspartate ABC transporter membrane subunit GltJ
RA 723,048 A→G V456A (GTA→GCA)  kdpD ← sensory histidine kinase KdpD
RA 864,282 C→T A214V (GCG→GTG)  fsaA → fructose‑6‑phosphate aldolase 1
RA 874,538 A→G K520K (AAA→AAG)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 918,956 G→A R55R (CGC→CGT)  ybjX ← DUF535 domain‑containing protein YbjX
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 996,394 T→A D184V (GAT→GTT)  ssuA ← aliphatic sulfonate ABC transporter periplasmic binding protein
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,016,897 T→C Q468R (CAG→CGG)  ycbZ ← putative ATP‑dependent protease YcbZ
RA 1,142,478 A→G T630T (ACT→ACC)  rne ← ribonuclease E
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,166,738 A→G E218E (GAA→GAG)  ndh → NADH:quinone oxidoreductase II
RA 1,175,955 C→T R177C (CGT→TGT)  lolC → lipoprotein release complex ‑ inner membrane subunit
RA 1,180,739 (G)6→7 coding (529/789 nt) ycfZ ← putative inner membrane protein
RA 1,212,088 (C)8→7 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,238,394 A→G Y275C (TAC→TGC)  dadA → D‑amino acid dehydrogenase
RA 1,240,196 A→G intergenic (+247/‑64) dadX → / → ymgM alanine racemase 2/protein YmgM
RA 1,244,639 A→G intergenic (‑112/‑89) ycgR ← / → ymgE flagellar brake protein YcgR/PF04226 family protein YmgE
RA 1,308,577 T→C V23V (GTA→GTG)  clsA ← cardiolipin synthase A
RA 1,328,833 C→T D94N (GAT→AAT)  yciK ← putative oxidoreductase
RA 1,339,054 A→G V36A (GTC→GCC)  ribA ← GTP cyclohydrolase 2
RA 1,362,537 C→T intergenic (+69/‑206) puuR → / → puuC DNA‑binding transcriptional repressor PuuR/gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
RA 1,388,635 A→G G59G (GGT→GGC)  tpx ← lipid hydroperoxide peroxidase
RA 1,399,675 A→G intergenic (‑149/+46) fnr ← / ← ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,422,316 A→G T112A (ACC→GCC)  ydaV → Rac prophage; putative ATP‑binding protein YdaV
RA 1,437,231 (T)9→10 intergenic (+2/+29) micC → / ← ydbK small regulatory RNA MicC/putative pyruvate‑flavodoxin oxidoreductase
RA 1,444,553 T→C H501H (CAT→CAC)  ydbH → PF11739 family protein YdbH
RA 1,446,045 G→A D56N (GAC→AAC)  ydbL → DUF1318 domain‑containing protein YdbL
RA 1,451,493 T→C intergenic (‑144/+104) tynA ← / ← paaZ copper‑containing amine oxidase/oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,458,689 A→G I142M (ATA→ATG)  paaG → putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,561,054 A→G D476D (GAT→GAC)  ddpA ← putative D,D‑dipeptide ABC transporter periplasmic binding protein
RA 1,580,210 G→A P194L (CCG→CTG)  ydeM ← putative anaerobic sulfatase maturation enzyme YdeM
RA 1,594,256 (C)8→9 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,604,471 A→G Y142C (TAC→TGC)  lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,628,250 T→C V245A (GTC→GCC)  ydfG → 3‑hydroxy acid dehydrogenase
RA 1,628,930 (A)5→6 coding (579/687 nt) ydfH → DNA‑binding transcriptional repressor YdfH
RA 1,631,544 Δ1 bp coding (742/1284 nt) ydfJ ← putative transporter YdfJ
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,674,901 G→A G330E (GGA→GAA)  tqsA → autoinducer 2 exporter
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,692,417 G→A G145G (GGC→GGT)  uidC ← outer membrane porin family protein
RA 1,703,538 T→C E257E (GAA→GAG)  ydgJ ← putative oxidoreductase YdgJ
RA 1,741,746 G→A A112T (GCC→ACC)  cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,857,330 A→G L147P (CTG→CCG)  ydjI ← putative aldolase YdjI
RA 1,873,384 A→G K448K (AAA→AAG)  dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,946,531 T→C V94A (GTT→GCT)  yebB → putative papain‑like amidase YebB
RA 1,949,276 T→C D416G (GAC→GGC)  aspS ← aspartate‑‑tRNA ligase
RA 1,951,982 G→A P196P (CCG→CCA)  yecE → DUF72 domain‑containing protein YecE
RA 1,976,588 G→A V184V (GTC→GTT)  motA ← motility protein A
RA 1,977,215 (A)8→7 intergenic (‑76/+51) motA ← / ← flhC motility protein A/DNA‑binding transcriptional dual regulator FlhC
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,037,311 T→C E281G (GAG→GGG)  hprS ← sensory histidine kinase HprS
RA 2,047,440 G→A G835S (GGC→AGC)  yeeJ → inverse autotransporter adhesin
RA 2,096,693 T→C V27A (GTA→GCA)  hisI → putative bifunctional phosphoribosyl‑AMP cyclohydrolase/phosphoribosyl‑ATP pyrophosphatase
RA 2,104,564 A→G V174A (GTC→GCC)  wbbJ ← putative acyl transferase
RA 2,107,568 T→C E254E (GAA→GAG)  glf ← UDP‑galactopyranose mutase
RA 2,124,657 C→T V364I (GTC→ATC)  wcaI ← putative colanic biosynthesis glycosyl transferase
RA 2,142,531 T→C T140A (ACC→GCC)  udk ← uridine/cytidine kinase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,214,839 C→T intergenic (‑195/‑27) btsS ← / → mlrA high‑affinity pyruvate receptor/DNA‑binding transcriptional activator MlrA
RA 2,218,611 (T)6→7 coding (871/918 nt) osmF ← glycine betaine ABC transporter periplasmic binding protein OsmF
RA 2,221,126 G→A H288H (CAC→CAT)  bglX ← beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,304,522 2 bp→TC intergenic (+129/+585) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,306,954 C→T intergenic (‑200/+18) mqo ← / ← yojI malate:quinone oxidoreductase/ABC transporter family protein/microcin J25 efflux protein
RA 2,322,813 G→A L316L (CTG→CTA)  atoC → DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC
RA 2,393,126 (G)5→6 intergenic (‑85/+79) nuoM ← / ← nuoL NADH:quinone oxidoreductase subunit M/NADH:quinone oxidoreductase subunit L
RA 2,419,382 (C)6→7 coding (407/555 nt) yfcE ← phosphodiesterase YfcE
RA 2,430,190 T→C I17V (ATT→GTT)  purF ← amidophosphoribosyltransferase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,512,699 T→C N3S (AAC→AGC)  mntH ← Mn(2(+))/Fe(2(+)): H(+) symporter MntH
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,529,863 T→C N105S (AAC→AGC)  ligA ← DNA ligase
RA 2,557,244 T→G G18G (GGA→GGC)  eutC ← ethanolamine ammonia‑lyase subunit beta
RA 2,563,545 G→A intergenic (+428/‑47) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein YffP/CPZ‑55 prophage; uncharacterized protein YffQ
RA 2,575,920 A→G intergenic (‑115/+178) eutS ← / ← maeB putative structural protein, ethanolamine utilization microcompartment/malate dehydrogenase
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,610,133 T→C R549R (CGT→CGC)  hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,690,033 T→C T356A (ACA→GCA)  glrK ← sensory histidine kinase GlrK
RA 2,693,393 A→G G717G (GGT→GGC)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,715,501 G→A A27T (GCC→ACC)  eamB → cysteine/O‑acetylserine exporter EamB
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,744,694 T→C T216A (ACC→GCC)  trmD ← tRNA m(1)G37 methyltransferase
RA 2,767,507 (C)7→8 intergenic (+152/‑203) rnlB → / → yfjP CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
RA 2,780,109 G→A A873V (GCC→GTC)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,788,007 T→C pseudogene (1611/2253 nt) ygaQ → putative uncharacterized protein YgaQ
RA 2,795,223 T→C V324A (GTA→GCA)  gabP → 4‑aminobutyrate:H(+) symporter
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,821,952 T→C G20G (GGA→GGG)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,832,059 T→C E77E (GAA→GAG)  norR ← DNA‑binding transcriptional dual regulator NorR
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,906,839 A→G I368T (ATC→ACC)  eno ← enolase
RA 2,914,300 G→A P234L (CCC→CTC)  rlmD ← 23S rRNA m(5)U1939 methyltransferase
RA 2,929,619 C→T S15F (TCC→TTC)  sdaB → L‑serine deaminase II
RA 2,941,730 Δ1 bp coding (838/918 nt) gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 2,942,298 A→G R90R (CGT→CGC)  gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 2,945,011 T→C intergenic (+26/+25) csdE → / ← tcdA sulfur acceptor protein CsdE/tRNA threonylcarbamoyladenosine dehydratase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,984,753 G→A S280S (AGC→AGT)  yqeF ← putative acyltransferase
RA 2,990,363 T→C intergenic (‑3/‑191) yqeK ← / → ygeF protein YqeK/protein YgeF
RA 3,020,790 C→T A84V (GCG→GTG)  ygfM → putative oxidoreductase
RA 3,042,827 C→T D108N (GAT→AAT)  yqfA ← hemolysin‑III family protein
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,085,636 G→A F92F (TTC→TTT)  speA ← arginine decarboxylase, biosynthetic
RA 3,104,287 G→A L65L (CTG→CTA)  yggX → putative Fe(2(+))‑trafficking protein
RA 3,108,796 G→A C120C (TGC→TGT)  speC ← ornithine decarboxylase, biosynthetic
RA 3,132,707 T→C Y169C (TAC→TGC)  yghR ← putative ATP‑binding protein YghR
RA 3,150,442 C→T Q261* (CAA→TAA)  yghA → NADP(+)‑dependent aldehyde reductase
RA 3,309,006 (C)6→7 intergenic (‑82/+27) nlpI ← / ← pnp lipoprotein NlpI/polynucleotide phosphorylase
RA 3,344,977 T→C S87S (AGT→AGC)  rpoN → RNA polymerase, sigma 54 (sigma N) factor
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,411,633 G→A intergenic (+66/‑20) fis → / → yhdJ DNA‑binding transcriptional dual regulator Fis/DNA adenine methyltransferase
RA 3,422,141 T→C W273R (TGG→CGG)  yhdY → putative ABC transporter membrane subunit YhdY
RA 3,428,918 Δ1 bp intergenic (‑156/‑318) rrsD ← / → yrdA 16S ribosomal RNA/protein YrdA
RA 3,428,923 Δ1 bp intergenic (‑161/‑313) rrsD ← / → yrdA 16S ribosomal RNA/protein YrdA
RA 3,428,927 T→G intergenic (‑165/‑309) rrsD ← / → yrdA 16S ribosomal RNA/protein YrdA
RA 3,429,258 A→G Y8C (TAC→TGC)  yrdA → protein YrdA
RA 3,541,548 A→G D381G (GAC→GGC)  feoB → Fe(2(+)) transporter FeoB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,576,951 (C)5→6 coding (111/1341 nt) gntU ← low‑affinity gluconate transporter
RA 3,577,648 A→G intergenic (‑56/+83) gntK ← / ← gntR D‑gluconate kinase, thermostable/DNA‑binding transcriptional repressor GntR
RA 3,589,257 C→T T257T (ACG→ACA)  ugpE ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
RA 3,590,501 G→A I137I (ATC→ATT)  ugpA ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
RA 3,657,779 A→G intergenic (+212/+207) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS
RA 3,699,049 G→A G279D (GGC→GAC)  bcsG → cellulose phosphoethanolamine transferase
RA 3,700,280 A→G intergenic (+81/‑283) rdlD → / → yhjV antisense regulatory RNA RdlD/putative transporter YhjV
RA 3,712,926 (T)8→7 coding (9/699 nt) yhjY ← conserved protein YhjY
RA 3,738,781 G→A A429T (GCC→ACC)  malS → alpha‑amylase
RA 3,796,188 (C)5→6 coding (210/960 nt) waaC → ADP‑heptose:LPS heptosyltransferase 1
RA 3,812,141 (A)6→7 coding (419/669 nt) yicR ← RadC‑like JAB domain‑containing protein YicR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,859,835 T→C L94L (TTA→TTG)  yidK ← putative transporter YidK
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,878,864 T→C E419G (GAA→GGA)  gyrB ← DNA gyrase subunit B
RA 3,931,183 (G)7→8 intergenic (‑90/‑133) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup
RA 3,934,196 G→A G140D (GGC→GAC)  rbsA → ribose ABC transporter ATP binding subunit
RA 4,021,782 C→T S519S (AGC→AGT)  ubiB → ubiquinone biosynthesis protein UbiB
RA 4,065,094 T→C I225V (ATT→GTT)  yihO ← putative sulfoquinovose transporter
RA 4,079,819 T→C R23R (CGT→CGC)  yiiF → protein YiiF
RA 4,082,540 C→T A74T (GCC→ACC)  fdoH ← formate dehydrogenase O subunit beta
RA 4,232,017 A→G V406A (GTA→GCA)  lysC ← aspartate kinase III
RA 4,242,594 T→C intergenic (‑340/+32) xylE ← / ← malG D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG
RA 4,267,446 C→T G111G (GGC→GGT)  tyrB → tyrosine aminotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,453 A→G V116A (GTT→GCT)  alsK ← D‑allose kinase
RA 4,309,080 Δ1 bp coding (390/981 nt) alsC ← D‑allose ABC transporter membrane subunit
RA 4,331,708 A→G T403A (ACG→GCG)  proP → osmolyte:H(+) symporter ProP
RA 4,355,144 A→G L423P (CTG→CCG)  dtpC ← dipeptide/tripeptide:H(+) symporter DtpC
RA 4,414,863 A→G T197A (ACC→GCC)  aidB → putative acyl‑CoA dehydrogenase AidB
RA 4,424,457 (A)8→9 intergenic (+71/+59) ulaF → / ← yjfY L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,486,942 A→G D242G (GAC→GGC)  lptF → lipopolysaccharide transport system protein LptF
RA 4,488,579 (C)7→9 coding (1485/1503 nt) yjgR ← DUF853 domain‑containing protein YjgR
RA 4,488,964 T→C D367G (GAT→GGT)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,503,169 (G)5→6 coding (263/1329 nt) insG ← KpLE2 phage‑like element; IS4 putative transposase
RA 4,561,940 T→C T246A (ACC→GCC)  yjiJ ← putative transporter YjiJ
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,637,700 C→T R335R (CGC→CGT)  creC → sensory histidine kinase CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423770–3424230 3424540–3424238 9–771 19 [13] [15] 16 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =5 (0.080)89 (1.510) 56/244 0.0 96.5% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 2 (0.030)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078055 (0.080)51 (0.870) 39/244 0.3 94.1% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 2 (0.030)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)73 (1.130) 50/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =95 (1.430)115 (1.740) 64/274 0.0 70.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)80 (1.200) 56/276 0.1 96.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA