breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 25,321 A→G T39A (ACG→GCG)  lspA → lipoprotein signal peptidase
RA 69,699 A→G V117A (GTA→GCA)  araB ← ribulokinase
RA 111,396 C→T A118V (GCC→GTC)  mutT → 8‑oxo‑dGTP diphosphatase
RA 132,805 C→T G397G (GGC→GGT)  acnB → hypothetical protein
RA 139,572 T→C T552A (ACC→GCC)  gcd ← quinoprotein glucose dehydrogenase
RA 147,917 G→A intergenic (+47/+27) rpnC → / ← panC recombination‑promoting nuclease RpnC/pantothenate synthetase
RA 151,398 T→C T68A (ACT→GCT)  yadK ← putative fimbrial protein YadK
RA 180,910 T→C S9S (AGT→AGC)  degP → periplasmic serine endoprotease DegP
RA 211,549 G→A W624* (TGG→TAG)  ldcC → lysine decarboxylase 2
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 265,858 A→G V77A (GTC→GCC)  ykfB ← CP4‑6 prophage; protein YkfB
RA 271,641 A→G T347A (ACT→GCT)  insI‑1 → IS30 transposase
RA 285,549 C→T S52F (TCC→TTC)  yagG → putative D‑xylonate transporter YagG
RA 286,071 A→G N226S (AAC→AGC)  yagG → putative D‑xylonate transporter YagG
RA 311,959 A→G intergenic (‑623/‑153) ecpR ← / → ykgL DNA‑binding transcriptional dual regulator MatA/uncharacterized protein YkgL
RA 328,735 A→G intergenic (‑2/+12) betB ← / ← betI betaine aldehyde dehydrogenase/DNA‑binding transcriptional repressor BetI
RA 331,144 A→G E561G (GAG→GGG)  betT → choline:H(+) symporter
RA 375,067 A→G intergenic (+186/‑392) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 388,679 G→A intergenic (+33/+74) tauD → / ← hemB taurine dioxygenase/porphobilinogen synthase
RA 451,129 A→G V161A (GTG→GCG)  cyoA ← cytochrome bo3 ubiquinol oxidase subunit 2
RA 462,711 C→T P266L (CCG→CTG)  ppiD → periplasmic folding chaperone
RA 505,352 A→G T147A (ACT→GCT)  ushA → 5'‑nucleotidase/UDP‑sugar hydrolase
RA 519,067 C→T G28D (GGC→GAC)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 560,790 A→G D365G (GAC→GGC)  sfmD → putative fimbrial usher protein SfmD
RA 670,177 A→G C132C (TGT→TGC)  nadD ← nicotinate‑mononucleotide adenylyltransferase
RA 706,490 A→G Y133C (TAC→TGC)  glnS → glutamine‑‑tRNA ligase
RA 749,491 (G)5→6 coding (217/729 nt) ybgP ← putative fimbrial chaperone YbgP
RA 775,872 T→C G8G (GGT→GGC)  tolR → Tol‑Pal system protein TolR
RA 780,523 A→G intergenic (+134/‑31) cpoB → / → lysT cell division coordinator CpoB/tRNA‑Lys
RA 811,376 A→G L332L (TTA→TTG)  bioF → 8‑amino‑7‑oxononanoate synthase
RA 829,262 T→C L237L (CTA→CTG)  ybhG ← HlyD_D23 family protein YbhG
RA 832,395 A→G intergenic (+159/+73) rhlE → / ← ybiA ATP‑dependent RNA helicase RhlE/N‑glycosidase YbiA
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 949,950 C→T P95S (CCG→TCG)  ycaK → putative NAD(P)H‑dependent oxidoreductase YcaK
RA 981,850 (G)6→7 coding (804/1848 nt) ldtD → L,D‑transpeptidase LdtD
RA 999,709 (C)6→7 coding (494/2601 nt) elfC → putative fimbrial usher protein ElfC
RA 1,003,306 C→T P140S (CCT→TCT)  ycbU → putative fimbrial protein YcbU
RA 1,007,916 (G)5→6 coding (73/2109 nt) rlmL → fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,053,326 G→A P13S (CCA→TCA)  yccM ← putative 4Fe‑4S membrane protein
RA 1,117,682 T→C C292C (TGT→TGC)  yceA → UPF0176 protein YceA
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,254,271 A→G V561A (GTA→GCA)  ycgV ← putative autotransporter adhesin YcgV
RA 1,291,997 G→A V181M (GTG→ATG)  galU → UTP‑‑glucose‑1‑phosphate uridylyltransferase
RA 1,318,571 (C)6→7 coding (1218/1362 nt) trpC ← fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
RA 1,324,113 (T)7→8 coding (16/621 nt) yciO → putative RNA‑binding protein YciO
RA 1,328,213 C→T A49T (GCA→ACA)  btuR ← cobinamide/cobalamin adenosyltransferase
RA 1,411,524 G→A A142V (GCG→GTG)  ttcA ← tRNA cytosine(32) 2‑sulfurtransferase TtcA
RA 1,419,721 (G)7→8 intergenic (‑265/+44) ydaG ← / ← racR Rac prophage; uncharacterized protein YdaG/Rac prophage; DNA‑binding transcriptional repressor RacR
RA 1,421,638 (C)7→8 coding (520/858 nt) ydaU → Rac prophage; DUF1376 domain‑containing protein YdaU
RA 1,456,980 A→G D184G (GAC→GGC)  paaE → phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit
RA 1,458,356 G→A M31I (ATG→ATA)  paaG → putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)
RA 1,463,046 A→G T308A (ACC→GCC)  paaK → phenylacetate‑CoA ligase
RA 1,478,176 A→G T186A (ACT→GCT)  ynbA → putative inner membrane protein
RA 1,536,408 G→A G70G (GGC→GGT)  narV ← nitrate reductase Z subunit gamma
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,554,497 T→C G391G (GGA→GGG)  maeA ← malate dehydrogenase, NAD‑requiring
RA 1,585,711 T→C N259S (AAC→AGC)  ydeP ← putative oxidoreductase YdeP
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,609,314 A→G V456A (GTG→GCG)  uxaB ← tagaturonate reductase
RA 1,632,770 T→C intergenic (‑485/‑120) ydfJ ← / → ynfT putative transporter YdfJ/Qin prophage; protein YnfT
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,675,348 A→G I338T (ATT→ACT)  pntB ← pyridine nucleotide transhydrogenase subunit beta
RA 1,675,478 T→C T295A (ACC→GCC)  pntB ← pyridine nucleotide transhydrogenase subunit beta
RA 1,679,346 C→T T307I (ACC→ATC)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,774,920 T→C F79L (TTT→CTT)  aroD → 3‑dehydroquinate dehydratase
RA 1,776,781 A→G T399A (ACC→GCC)  ydiF → putative acetate‑CoA transferase
RA 1,805,322 A→G intergenic (+50/+3) yniD → / ← ydiY uncharacterized protein YniD/acid‑inducible putative outer membrane protein YdiY
JC 1,806,042 (C)6→11 coding (42/759 nt) ydiY ← acid‑inducible putative outer membrane protein YdiY
RA 1,829,083 A→G F218L (TTT→CTT)  astD ← aldehyde dehydrogenase
RA 1,863,508 T→C R246R (CGT→CGC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,863,778 A→G intergenic (+12/‑72) gapA → / → yeaD glyceraldehyde‑3‑phosphate dehydrogenase A/putative aldose 1‑epimerase YeaD
RA 1,873,384 A→G K448K (AAA→AAG)  dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,904,787 (T)7→8 intergenic (+41/‑14) manZ → / → yobD mannose‑specific PTS enzyme IID component/conserved inner membrane protein YobD
RA 1,919,819 T→C G770G (GGT→GGC)  yebT → intermembrane transport protein YebT
RA 1,931,719 T→C V280A (GTC→GCC)  purT → phosphoribosylglycinamide formyltransferase 2
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,017,729 A→G K392K (AAA→AAG)  fliI → flagellum‑specific ATP synthase FliI
RA 2,021,372 A→G E95E (GAA→GAG)  fliN → flagellar motor switch protein FliN
RA 2,087,293 A→G intergenic (‑179/+36) yoeI ← / ← yeeY uncharacterized protein YoeI/putative DNA‑binding transcriptional regulator YeeY
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,283,721 T→C D76G (GAC→GGC)  yejK ← nucleoid‑associated protein YejK
RA 2,295,140 T→C T81A (ACC→GCC)  ccmE ← periplasmic heme chaperone
RA 2,405,158 (A)8→7 intergenic (‑86/+545) nuoA ← / ← lrhA NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,503,862 (C)5→4 coding (624/2496 nt) fryA ← putative PTS multiphosphoryl transfer protein FryA
RA 2,511,651 A→G S352S (AGT→AGC)  mntH ← Mn(2(+))/Fe(2(+)): H(+) symporter MntH
RA 2,524,895 T→C intergenic (‑17/‑232) xapA ← / → yfeN xanthosine phosphorylase/conserved outer membrane protein YfeN
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,558,974 T→C G39G (GGT→GGC)  intZ → CPZ‑55 prophage; putative phage integrase IntZ
RA 2,646,629 A→G S233P (TCC→CCC)  pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,684,763 A→G Y249H (TAC→CAC)  glyA ← serine hydroxymethyltransferase
RA 2,801,061 C→T R33W (CGG→TGG)  nrdI → dimanganese‑tyrosyl radical cofactor maintenance flavodoxin NrdI
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,813,274 T→C L220P (CTC→CCC)  emrB → multidrug efflux pump membrane subunit EmrB
RA 2,821,424 G→C G196G (GGC→GGG)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,982,660 C→T R200Q (CGG→CAG)  kduD ← putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase
RA 2,995,369 T→C pseudogene (652/707 nt) ygeN ← putative type III secretion system protein
RA 3,006,734 A→G N158S (AAC→AGC)  ygeW → putative carbamoyltransferase YgeW
RA 3,060,065 T→C W105R (TGG→CGG)  argP → DNA‑binding transcriptional dual regulator ArgP
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,077,201 (T)6→7 coding (256/1278 nt) yggP ← putative zinc‑binding dehydrogenase YggP
RA 3,121,626 (T)6→7 intergenic (‑347/+8) glcA ← / ← glcB glycolate/lactate:H(+) symporter GlcA/malate synthase G
RA 3,179,549 T→C S479P (TCC→CCC)  tolC → outer membrane channel TolC
RA 3,182,807 A→G P86P (CCA→CCG)  zupT → heavy metal divalent cation transporter ZupT
RA 3,244,483 C→T A87T (GCC→ACC)  uxaC ← D‑glucoronate/D‑galacturonate isomerase
RA 3,263,000 T→C T221A (ACC→GCC)  tdcD ← propionate kinase
RA 3,287,806 T→C G127G (GGT→GGC)  yraH → putative fimbrial protein YraH
RA 3,318,030 (A)7→8 intergenic (‑24/+183) rimP ← / ← metY ribosome maturation factor RimP/tRNA‑Met
RA 3,334,451 A→G T248A (ACA→GCA)  ispB → all‑trans‑octaprenyl‑diphosphate synthase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,627,323 A→G V739A (GTG→GCG)  rbbA ← ribosome‑associated ATPase
RA 3,689,387 G→A A292V (GCG→GTG)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,701,665 T→C V368A (GTG→GCG)  yhjV → putative transporter YhjV
RA 3,739,251 C→T G585G (GGC→GGT)  malS → alpha‑amylase
RA 3,739,600 T→C intergenic (+73/‑105) malS → / → avtA alpha‑amylase/valine‑‑pyruvate aminotransferase
RA 3,760,127 +A coding (1123/1392 nt) selA ← selenocysteine synthase
RA 3,765,703 G→A G1174D (GGC→GAC)  rhsA → rhs element protein RhsA
RA 3,793,904 T→C intergenic (‑221/‑83) htrL ← / → rfaD protein HtrL/ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
RA 3,798,933 G→A P127L (CCC→CTC)  waaU ← putative ADP‑heptose:LPS heptosyltransferase 4
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,824,046 A→G P549P (CCA→CCG)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,882,325 T→C intergenic (‑4/+1) dnaN ← / ← dnaA beta sliding clamp/chromosomal replication initiator protein DnaA
RA 3,965,013 A→G R206R (CGT→CGC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 4,002,708 (C)6→7 coding (92/381 nt) yigF ← DUF2628 domain‑containing protein YigF
RA 4,096,292 A→G L386P (CTG→CCG)  rhaB ← rhamnulokinase
RA 4,111,623 T→C N198D (AAT→GAT)  yiiQ ← DUF1454 domain‑containing protein YiiQ
RA 4,200,176 (G)6→7 intergenic (+82/‑105) yjaG → / → hupA conserved protein YjaG/DNA‑binding protein HU‑alpha
RA 4,204,349 +C coding (1030/1326 nt) zraR → DNA‑binding transcriptional activator ZraR
RA 4,255,651 G→A G272G (GGC→GGT)  plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,957 A→G G174G (GGT→GGC)  alsE ← D‑allulose‑6‑phosphate 3‑epimerase
RA 4,308,118 C→T A121T (GCC→ACC)  alsE ← D‑allulose‑6‑phosphate 3‑epimerase
RA 4,312,224 T→C L256L (TTA→TTG)  alsR ← DNA‑binding transcriptional repressor AlsR
RA 4,316,487 G→A G244G (GGC→GGT)  phnM ← RPnTP hydrolase
RA 4,339,493 A→G R343R (CGT→CGC)  adiA ← arginine decarboxylase, degradative
RA 4,398,578 (C)5→6 coding (1167/1848 nt) mutL → DNA mismatch repair protein MutL
RA 4,416,802 (G)5→6 intergenic (‑32/‑150) bsmA ← / → yjfP DUF1471 domain‑containing putative lipoprotein BsmA/carboxylesterase
RA 4,439,465 (A)7→8 intergenic (‑203/‑122) ytfJ ← / → ytfK protein YtfJ/DUF1107 domain‑containing protein YtfK
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,475,633 G→A G66D (GGT→GAT)  yjgL → protein YjgL
RA 4,550,069 A→G F410L (TTC→CTC)  gntP ← fructuronate transporter
RA 4,556,769 (T)9→10 coding (116/144 nt) ytiD → protein YtiD
RA 4,558,573 G→T V318V (GTC→GTA)  iadA ← isoaspartyl dipeptidase
RA 4,558,653 A→G F292L (TTC→CTC)  iadA ← isoaspartyl dipeptidase
RA 4,583,210 C→T intergenic (‑162/+39) hsdM ← / ← hsdR type I restriction enzyme EcoKI methyltransferase component/type I restriction enzyme EcoKI endonuclease component
RA 4,606,094 (G)8→7 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,619,519 A→G T431A (ACA→GCA)  deoA → thymidine phosphorylase
RA 4,631,527 A→G P265P (CCA→CCG)  slt → soluble lytic murein transglycosylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1632826–1632865 1633546–1633224 360–721 20 [17] [18] 19 ynfT–ydfK ynfT,ydfK
* * ÷ NC_000913 3423763–3424234 3424525–3424241 8–763 19 [18] [18] 21 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4038838–4039382 4040514–4039392 11–1677 19 [18] [18] 19 [rrlA] [rrlA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =81 (1.070)19 (0.280) 15/244 2.6 23.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 51 (0.760)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)80 (1.080) 45/268 0.4 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =116 (1.530)131 (1.740) 68/274 0.0 68.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)87 (1.140) 62/276 0.1 94.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA