breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 159 A→G intergenic (–/‑31) – / → thrL –/thr operon leader peptide
RA 92,770 T→C V453A (GTT→GCT)  ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 101,209 A→G T149A (ACC→GCC)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 103,619 G→A Q155Q (CAG→CAA)  ftsQ → cell division protein FtsQ
RA 106,748 G→A T64T (ACG→ACA)  lpxC → UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase
RA 113,959 T→C G172G (GGT→GGC)  guaC → GMP reductase
RA 129,575 A→G R562R (CGT→CGC)  yacH ← DUF3300 domain‑containing protein YacH
RA 140,912 A→G I105T (ATC→ACC)  gcd ← quinoprotein glucose dehydrogenase
RA 147,901 T→C intergenic (+31/+43) rpnC → / ← panC recombination‑promoting nuclease RpnC/pantothenate synthetase
RA 149,662 (G)5→4 intergenic (‑61/+53) panB ← / ← yadC 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial tip‑adhesin YadC
RA 154,784 C→T A215T (GCC→ACC)  htrE ← putative fimbrial usher protein HtrE
RA 156,672 T→C D71G (GAC→GGC)  yadN ← putative fimbrial protein YadN
RA 209,049 A→G A143A (GCA→GCG)  accA → acetyl‑CoA carboxyltransferase subunit alpha
RA 220,801 T→C Q43R (CAG→CGG)  metQ ← L‑methionine/D‑methionine ABC transporter membrane anchored binding protein
RA 230,935 C→T intergenic (‑54/‑187) yafC ← / → yafD putative LysR family transcriptional regulator YafC/endonuclease/exonuclease/phosphatase domain‑containing protein YafD
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 276,394 C→T A357V (GCC→GTC)  mmuP → S‑methyl‑L‑methionine transporter
RA 279,227 T→A Q152L (CAG→CTG)  insB‑2 ← IS1 protein InsB
RA 300,801 (G)5→6 coding (134/2199 nt) paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 302,807 C→T intergenic (‑234/‑184) paoA ← / → yagU aldehyde dehydrogenase, Fe‑S subunit/inner membrane protein that contributes to acid resistance
RA 334,384 G→A A17V (GCG→GTG)  yahB ← putative LysR‑type DNA‑binding transcriptional regulator YahB
RA 364,488 A→G G606G (GGT→GGC)  lacZ ← beta‑galactosidase
RA 407,501 A→G Y25C (TAC→TGC)  aroM → protein AroM
RA 453,382 A→G V55A (GTA→GCA)  ampG ← muropeptide:H(+) symporter
RA 497,853 (C)5→4 intergenic (+34/‑202) adk → / → hemH adenylate kinase/ferrochelatase
RA 522,018 A→G T535A (ACA→GCA)  ybbP → putative ABC transporter membrane subunit YbbP
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 542,354 C→T A156V (GCC→GTC)  glxK → glycerate 2‑kinase 2
RA 550,730 A→G M98V (ATG→GTG)  ybcF → putative carbamate kinase
RA 606,453 T→C T311A (ACC→GCC)  ybdK ← carboxylate‑amine ligase
RA 663,161 A→G V268A (GTA→GCA)  dacA ← D‑alanyl‑D‑alanine carboxypeptidase DacA
RA 729,098 C→T intergenic (‑277/‑36) kdpF ← / → ybfA K(+) transporting P‑type ATPase subunit KdpF/DUF2517 domain‑containing protein YbfA
RA 746,247 A→G S105G (AGC→GGC)  nei → endonuclease VIII
RA 775,366 A→G K72E (AAA→GAA)  tolQ → Tol‑Pal system protein TolQ
RA 777,431 A→G T364A (ACG→GCG)  tolA → Tol‑Pal system protein TolA
RA 805,415 G→A G638D (GGC→GAC)  ybhJ → putative hydratase YbhJ
RA 822,424 C→T V25V (GTG→GTA)  ybhN ← conserved inner membrane protein YbhN
RA 868,480 A→G T321A (ACC→GCC)  gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 872,495 T→C I202I (ATT→ATC)  gsiD → glutathione ABC transporter membrane subunit GsiD
RA 874,444 A→G E489G (GAA→GGA)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 883,918 T→C R82R (CGT→CGC)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 907,475 G→A L270L (CTG→TTG)  ybjT ← putative NAD(P)‑dependent oxidoreductase YbjT
RA 931,769 C→T P94P (CCG→CCA)  trxB ← thioredoxin reductase
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 973,211 C→T R64H (CGT→CAT)  elyC ← envelope biogenesis factor
RA 976,162 A→G T181A (ACC→GCC)  mukE → chromosome partitioning protein MukE
RA 992,541 A→G V640V (GTA→GTG)  pepN → aminopeptidase N
RA 1,002,236 C→T H144Y (CAT→TAT)  elfG → putative fimbrial‑like adhesin protein
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,043,741 A→G V157A (GTT→GCT)  etk ← protein‑tyrosine kinase Etk
RA 1,066,855 A→G intergenic (+29/+9) agp → / ← yccJ glucose‑1‑phosphatase/PF13993 family protein YccJ
RA 1,078,343 G→A T180T (ACC→ACT)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,093,358 (G)6→5 coding (483/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,096,687 G→A W282* (TGG→TAG)  ycdU → uncharacterized protein YcdU
RA 1,103,216 (T)7→8 intergenic (‑20/‑735) csgD ← / → csgB DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit
RA 1,140,414 A→G T128A (ACT→GCT)  flgL → flagellar hook‑filament junction protein 2
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,145,876 C→T Q313* (CAA→TAA)  rluC → 23S rRNA pseudouridine(955/2504/2580) synthase
RA 1,159,853 A→G S567P (TCC→CCC)  fhuE ← ferric coprogen/ferric rhodotorulic acid outer membrane transporter
RA 1,176,647 A→G N10S (AAC→AGC)  lolD → lipoprotein release complex ‑ ATP binding subunit
RA 1,195,989 C→T I289I (ATC→ATT)  icd → isocitrate dehydrogenase
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,274,892 T→C V370A (GTG→GCG)  ychO → putative invasin YchO
RA 1,341,395 A→G T280A (ACC→GCC)  lapB → lipopolysaccharide assembly protein B
RA 1,356,351 C→T G254S (GGC→AGC)  sapA ← putative periplasmic binding protein SapA
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,378,150 C→T A89A (GCC→GCT)  ycjT → kojibiose phosphorylase
RA 1,394,686 A→G N487S (AAC→AGC)  mppA → murein tripeptide ABC transporter periplasmic binding protein
RA 1,420,793 (G)6→8 coding (110/423 nt) ydaT → Rac prophage; protein YdaT
RA 1,425,603 T→C V76A (GTT→GCT)  ynaK → Rac prophage; ParB‑like nuclease domain‑containing protein YnaK
RA 1,449,340 T→C V670V (GTA→GTG)  tynA ← copper‑containing amine oxidase
RA 1,452,425 T→C A406A (GCA→GCG)  paaZ ← oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,470,785 C→T pseudogene (267/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,512,239 T→G L196V (TTG→GTG)  ydcS → putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,541,596 C→T R332H (CGC→CAC)  narZ ← nitrate reductase Z subunit alpha
RA 1,568,560 T→C Q88Q (CAA→CAG)  yddW ← putative lipoprotein YddW
RA 1,594,256 (C)8→10 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,601,601 (G)5→6 coding (112/1536 nt) lsrA → Autoinducer‑2 ABC transporter ATP binding subunit
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,689,211 A→G E212E (GAA→GAG)  manA → mannose‑6‑phosphate isomerase
RA 1,696,376 A→G intergenic (‑305/+86) uidA ← / ← uidR beta‑glucuronidase/DNA‑binding transcriptional repressor UidR
RA 1,714,386 T→C F4L (TTC→CTC)  gstA → glutathione S‑transferase GstA
RA 1,765,577 T→C N19S (AAC→AGC)  menI ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,808,148 A→G D118G (GAC→GGC)  yniA → putative kinase YniA
RA 1,862,296 T→C H45R (CAC→CGC)  msrB ← methionine sulfoxide reductase B
RA 1,873,384 A→G K448K (AAA→AAG)  dgcJ → putative diguanylate cyclase DgcJ
RA 1,879,225 G→A L11F (CTT→TTT)  yeaQ ← PF04226 family protein YeaQ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,880,220 C→G Q180H (CAG→CAC)  leuE ← leucine exporter
RA 1,882,057 A→G E49G (GAG→GGG)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,888,409 T→C G446G (GGA→GGG)  fadD ← fatty acyl‑CoA synthetase
RA 1,890,467 A→G G22G (GGT→GGC)  yeaY ← Slp family lipoprotein YeaY
RA 1,915,961 T→C V56V (GTA→GTG)  msrC ← free methionine‑(R)‑sulfoxide reductase
RA 1,941,755 T→C T277A (ACG→GCG)  znuA ← Zn(2(+)) ABC transporter periplasmic binding protein
RA 1,945,195 G→A A54A (GCC→GCT)  ruvB ← Holliday junction branch migration complex subunit RuvB
RA 1,946,665 A→C T139P (ACG→CCG)  yebB → putative papain‑like amidase YebB
RA 1,954,576 T→C intergenic (+163/+2) cmoB → / ← torZ tRNA U34 carboxymethyltransferase/trimethylamine N‑oxide reductase 2
RA 1,956,094 T→C Q305R (CAG→CGG)  torZ ← trimethylamine N‑oxide reductase 2
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 1,997,416 C→T G133G (GGG→GGA)  tcyN ← cystine ABC transporter ATP binding subunit
RA 2,025,579 T→C S23S (AGT→AGC)  yedP → putative mannosyl‑3‑phosphoglycerate phosphatase
RA 2,074,362 (C)6→7 coding (2824/3120 nt) flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,107,310 A→G R340R (CGT→CGC)  glf ← UDP‑galactopyranose mutase
RA 2,165,573 (C)6→7 intergenic (+52/‑95) yegP → / → yegQ DUF1508 domain‑containing protein YegP/putative peptidase YegQ
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,237,065 T→C N230S (AAC→AGC)  mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
RA 2,238,041 T→C I417V (ATT→GTT)  mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
RA 2,245,388 T→C Y461C (TAC→TGC)  cirA ← ferric dihyroxybenzoylserine outer membrane transporter
RA 2,309,762 A→G L215P (CTG→CCG)  ada ← DNA‑binding transcriptional dual regulator/DNA repair protein Ada
RA 2,336,441 G→A S68S (AGC→AGT)  yfaA ← DUF2138 domain‑containing protein YfaA
RA 2,337,728 T→C K565E (AAA→GAA)  gyrA ← DNA gyrase subunit A
RA 2,379,360 T→C L428L (TTA→TTG)  menF ← isochorismate synthase MenF
RA 2,394,460 T→C N196S (AAC→AGC)  nuoL ← NADH:quinone oxidoreductase subunit L
RA 2,431,067 T→C Q206Q (CAA→CAG)  dedD ← cell division protein DedD
RA 2,436,415 T→C T79A (ACC→GCC)  usg ← putative semialdehyde dehydrogenase Usg
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→10 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,473,350 G→A intergenic (‑104/‑254) yfdO ← / → yfdP CPS‑53 (KpLE1) prophage; putative defective phage replication protein O/CPS‑53 (KpLE1) prophage; protein YfdP
RA 2,492,667 G→A V196V (GTC→GTT)  frc ← formyl‑CoA transferase
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,531,591 G→A M43I (ATG→ATA)  cysZ → sulfate:H(+) symporter
RA 2,550,910 T→C T103A (ACG→GCG)  yfeY ← DUF1131 domain‑containing lipoprotein YfeY
RA 2,561,890 G→A R89H (CGC→CAC)  yffN → CPZ‑55 prophage; uncharacterized protein YffN
RA 2,578,847 (A)8→9 coding (182/951 nt) talA → transaldolase A
RA 2,591,002 T→C intergenic (‑136/‑245) ypfM ← / → yffB uncharacterized protein YpfM/putative reductase YffB
RA 2,601,608 A→G E136E (GAA→GAG)  hyfA → hydrogenase 4 component A
RA 2,614,706 T→C M258T (ATG→ACG)  focB → putative formate transporter
RA 2,615,702 T→C Q60Q (CAA→CAG)  yfgO ← putative transporter YfgO
RA 2,624,731 T→C V563A (GTT→GCT)  ppk → polyphosphate kinase
RA 2,625,117 G→A M1M (ATG→ATA) † ppx → exopolyphosphatase
RA 2,625,141 T→C R9R (CGT→CGC)  ppx → exopolyphosphatase
RA 2,640,662 A→G intergenic (‑87/+24) hisS ← / ← ispG histidine‑‑tRNA ligase/(E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin)
RA 2,644,561 T→C T102A (ACT→GCT)  ndk ← nucleoside diphosphate kinase
RA 2,645,282 G→A P682S (CCG→TCG)  pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,666,093 T→C V220A (GTT→GCT)  csiE → stationary phase inducible protein CsiE
RA 2,698,929 (A)5→6 coding (171/849 nt) yfhH → putative DNA‑binding transcriptional regulator YfhH
RA 2,699,776 T→C C38C (TGT→TGC)  yfhL → putative 4Fe‑4S cluster‑containing protein YfhL
RA 2,707,690 G→A R38C (CGC→TGC)  rseC ← protein RseC
RA 2,738,583 T→C F280S (TTT→TCT)  pheA → fused chorismate mutase/prephenate dehydratase
JC 2,767,507 (C)7→10 intergenic (+152/‑203) rnlB → / → yfjP CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
RA 2,769,457 G→A A265T (GCA→ACA)  yfjQ → CP4‑57 prophage; protein YfjQ
RA 2,791,898 T→C V209A (GTC→GCC)  gabD → NADP(+)‑dependent succinate‑semialdehyde dehydrogenase
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,832,355 G→A intergenic (‑66/‑121) norR ← / → norV DNA‑binding transcriptional dual regulator NorR/anaerobic nitric oxide reductase flavorubredoxin
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,888,020 T→C K98R (AAG→AGG)  cysH ← phosphoadenosine phosphosulfate reductase
RA 2,890,711 T→C E396E (GAA→GAG)  cysJ ← sulfite reductase, flavoprotein subunit
RA 2,897,037 A→G G278G (GGT→GGC)  ygcS ← putative transporter YgcS
RA 2,898,921 T→C T166T (ACA→ACG)  ygcU ← putative FAD‑containing dehydrogenase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 3,013,671 G→A P190L (CCC→CTC)  yqeB ← XdhC‑CoxI family protein YqeB
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,074,585 T→C L35L (TTA→TTG)  yggC ← P‑loop NTPase domain‑containing protein YggC
RA 3,076,370 (T)6→7 coding (1087/1278 nt) yggP ← putative zinc‑binding dehydrogenase YggP
RA 3,083,475 T→C H108R (CAC→CGC)  speB ← agmatinase
RA 3,093,932 T→C intergenic (+16/+168) yqgF → / ← yggR ribonuclease H‑like domain containing nuclease/Type II/IV secretion system family protein
RA 3,115,233 T→C T1294A (ACG→GCG)  yghJ ← putative lipoprotein YghJ
RA 3,134,803 A→G T225A (ACC→GCC)  yghT → putative ATP‑binding protein YghT
RA 3,158,553 (A)5→6 coding (904/927 nt) yqhG → DUF3828 domain‑containing protein YqhG
RA 3,195,534 T→C D407G (GAC→GGC)  rfaE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
RA 3,238,067 T→C S253P (TCT→CCT)  ygjR → putative oxidoreductase YgjR
RA 3,240,984 (G)7→8 coding (1041/1245 nt) sstT → serine/threonine:Na(+) symporter
RA 3,264,839 T→A Y60F (TAC→TTC)  tdcC ← threonine/serine:H(+) symporter
RA 3,265,564 A→T D155E (GAT→GAA)  tdcB ← catabolic threonine dehydratase
RA 3,267,015 T→C E17E (GAA→GAG)  tdcA ← DNA‑binding transcriptional activator TdcA
RA 3,289,090 A→G T93A (ACA→GCA)  yraJ → putative fimbrial usher protein YraJ
RA 3,304,813 (C)7→9 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,312,238 G→A I180I (ATC→ATT)  truB ← tRNA pseudouridine(55) synthase
RA 3,321,189 A→G V142A (GTG→GCG)  yhbX ← putative hydrolase, inner membrane
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,369,224 C→T M85I (ATG→ATA)  yhcH ← DUF386 domain‑containing protein YhcH
RA 3,379,266 (C)7→6 coding (732/1128 nt) zapE ← cell division factor ZapE with ATPase activity
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,389,676 A→G T53A (ACA→GCA)  aaeR → LysR‑type transcriptional regulator AaeR
RA 3,465,072 A→G N384S (AAC→AGC)  gspL → Type II secretion system protein GspL
RA 3,538,348 G→C A322P (GCC→CCC)  yhgF → putative RNA‑binding protein YhgF
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,605,983 A→G Y78C (TAC→TGC)  yhhN → conserved inner membrane enzyme YhhN
RA 3,612,265 T→C V134A (GTT→GCT)  yhhT → putative transporter YhhT
RA 3,619,119 T→A intergenic (+130/‑73) nikR → / → rhsB DNA‑binding transcriptional repressor NikR/rhs element protein RhsB
RA 3,632,224 T→C N123D (AAT→GAT)  yhiJ ← DUF4049 domain‑containing protein YhiJ
RA 3,636,286 (C)6→5 coding (1125/1203 nt) yhiN ← putative oxidoreductase YhiN
RA 3,668,536 A→G V218A (GTC→GCC)  ccp ← cytochrome c peroxidase
RA 3,673,861 T→C V167A (GTC→GCC)  yhjD → putative transporter YhjD
RA 3,685,008 T→C H204R (CAC→CGC)  pdeK ← putative c‑di‑GMP phosphodiesterase PdeK
RA 3,694,567 A→G W224R (TGG→CGG)  bcsA ← cellulose synthase catalytic subunit
RA 3,696,540 T→C V28A (GTC→GCC)  bcsE → c‑di‑GMP‑binding protein BcsE
RA 3,699,879 C→T P556S (CCT→TCT)  bcsG → cellulose phosphoethanolamine transferase
RA 3,718,895 T→C E50E (GAA→GAG)  yiaF ← DUF3053 domain‑containing protein YiaF
RA 3,724,951 T→C N123S (AAC→AGC)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,603 G→A S388F (TCC→TTC)  xylA ← xylose isomerase
RA 3,743,007 (A)5→4 coding (275/999 nt) yiaK → 2,3‑diketo‑L‑gulonate reductase
RA 3,795,662 A→G N245S (AAC→AGC)  waaF → ADP‑heptose‑‑LPS heptosyltransferase 2
RA 3,801,790 (A)7→6 coding (210/1017 nt) waaJ ← UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,818,188 T→C H143H (CAT→CAC)  dinD → DNA damage‑inducible protein D
RA 3,833,939 T→C Y200C (TAC→TGC)  yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,873,948 C→T G176D (GGT→GAT)  dgoK ← 2‑dehydro‑3‑deoxygalactonokinase
RA 3,924,470 A→G G388G (GGT→GGC)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 3,954,120 C→A Q215K (CAG→AAG)  ilvD → dihydroxy‑acid dehydratase
RA 3,956,754 T→C V475A (GTA→GCA)  ilvA → threonine deaminase
RA 3,970,845 A→G D238G (GAC→GGC)  wecB → UDP‑N‑acetylglucosamine 2‑epimerase
RA 4,104,535 A→G R147R (CGT→CGC)  cpxA ← sensory histidine kinase CpxA
RA 4,104,922 C→T A18A (GCG→GCA)  cpxA ← sensory histidine kinase CpxA
RA 4,157,986 A→G V379V (GTA→GTG)  argH → argininosuccinate lyase
RA 4,190,856 T→C Q338R (CAG→CGG)  thiH ← 2‑iminoacetate synthase
RA 4,199,676 C→T T58M (ACG→ATG)  yjaG → conserved protein YjaG
RA 4,261,093 A→G G165G (GGA→GGG)  yjbM → uncharacterized protein YjbM
RA 4,273,541 A→G L111L (TTA→CTA)  uvrA ← excision nuclease subunit A
RA 4,275,415 A→G intergenic (‑374/‑56) yjcB ← / → pdeC uncharacterized protein YjcB/c‑di‑GMP phosphodiesterase PdeC
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,310,738 A→G G81G (GGT→GGC)  alsA ← D‑allose ABC transporter ATP binding subunit
RA 4,330,361 A→G intergenic (+123/‑141) yjcZ → / → proP uncharacterized protein YjcZ/osmolyte:H(+) symporter ProP
RA 4,331,389 C→T H296H (CAC→CAT)  proP → osmolyte:H(+) symporter ProP
RA 4,416,352 G→A intergenic (‑60/+89) yjfN ← / ← bsmA protease activator/DUF1471 domain‑containing putative lipoprotein BsmA
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,480,101 A→G L124L (TTA→TTG)  yjgN → conserved inner membrane protein YjgN
JC 4,517,951 (C)6→9 coding (285/522 nt) fecI ← RNA polymerase sigma factor FecI
RA 4,566,339 (C)6→7 coding (908/1281 nt) yjiN ← DUF445 domain‑containing protein YjiN
RA 4,608,268 T→C S28S (AGT→AGC)  rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase
RA 4,609,828 A→G T139A (ACC→GCC)  prfC → peptide chain release factor RF3
RA 4,619,309 A→G M361V (ATG→GTG)  deoA → thymidine phosphorylase
RA 4,639,160 T→C L328P (CTG→CCG)  creD → putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1209603 1209618 16 52 [0] [0] 85 stfE e14 prophage; putative side tail fiber protein fragment
* * ÷ NC_000913 3423750–3424234 3424523–3424239 6–774 16 [12] [11] 13 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3619350–3621077 3622203–3621087 11–2854 15 [12] [11] 14 rhsB rhs element protein RhsB

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =0 (0.000)72 (1.570) 43/244 0.1 100% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 0 (0.000)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078050 (0.000)44 (0.960) 34/244 0.3 100% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 0 (0.000)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)65 (1.290) 48/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =90 (1.730)84 (1.630) 50/274 0.1 65.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)36 (0.690) 28/276 0.7 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 454269039 (0.750)13 (0.270) 12/258 2.4 24.7% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 43 (0.890)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit