![]() |
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 31,404 | G→A | L196L (CTG→CTA) | carB → | carbamoyl phosphate synthetase subunit beta |
| RA | 53,858 | A→G | V282A (GTG→GCG) | surA ← | peptidyl‑prolyl cis‑trans isomerase SurA |
| RA | 63,566 | G→A | P739S (CCG→TCG) | polB ← | DNA polymerase II |
| RA | 160,637 | +C | intergenic (‑33/+145) | dksA ← / ← sfsA | RNA polymerase‑binding transcription factor DksA/putative DNA‑binding transcriptional regulator of maltose metabolism |
| RA | 206,530 | A→G | M469V (ATG→GTG) | dnaE → | DNA polymerase III subunit alpha |
| RA | 228,763 | C→T | noncoding (8/120 nt) | rrfH → | 5S ribosomal RNA |
| MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
| RA | 308,054 | (C)7→8 | coding (1279/2526 nt) | ecpC ← | putative fimbrial usher protein EcpC |
| RA | 343,463 | G→A | A194T (GCC→ACC) | yahK → | aldehyde reductase, NADPH‑dependent |
| RA | 380,022 | (G)10→9 | intergenic (‑141/+47) | frmR ← / ← yaiO | DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO |
| RA | 455,437 | A→G | D102G (GAC→GGC) | tig → | trigger factor |
| RA | 466,933 | T→C | Y127C (TAC→TGC) | ybaE ← | putative protein YbaE |
| RA | 474,489 | Δ1 bp | coding (673/861 nt) | tesB ← | acyl‑CoA thioesterase II |
| RA | 510,590 | A→G | W264R (TGG→CGG) | copA ← | Cu(+) exporting P‑type ATPase |
| RA | 522,308 | A→G | Q631Q (CAA→CAG) | ybbP → | putative ABC transporter membrane subunit YbbP |
| RA | 527,577 | T→C | intergenic (+36/‑4) | rhsD → / → ybbC | protein RhsD/PF15631 family protein YbbC |
| RA | 528,234 | C→T | pseudogene (286/711 nt) | ylbH → | putative RHS domain‑containing protein YlbH |
| RA | 547,554 | C→T | A292V (GCG→GTG) | fdrA → | putative acyl‑CoA synthetase FdrA |
| RA | 554,220 | T→C | N73S (AAC→AGC) | ppiB ← | peptidyl‑prolyl cis‑trans isomerase B |
| RA | 606,120 | (G)5→6 | coding (80/249 nt) | ybdJ ← | DUF1158 domain‑containing protein YbdJ |
| RA | 654,886 | C→T | A361A (GCG→GCA) | dcuC ← | anaerobic C4‑dicarboxylate transporter DcuC |
| RA | 717,125 | (G)6→7 | coding (1141/1320 nt) | potE ← | putrescine:H(+) symporter/putrescine:ornithine antiporter PotE |
| RA | 721,645 | T→G | E30A (GAG→GCG) | kdpE ← | DNA‑binding transcriptional activator KdpE |
| RA | 751,514 | (A)5→4 | coding (656/2448 nt) | ybgQ ← | putative fimbrial usher protein YbgQ |
| RA | 751,737 | (G)6→7 | coding (433/2448 nt) | ybgQ ← | putative fimbrial usher protein YbgQ |
| RA | 766,957 | (G)6→7 | coding (974/1977 nt) | mngA → | 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II |
| RA | 769,676 | T→C | Y567H (TAT→CAT) | mngB → | alpha‑mannosidase |
| RA | 846,947 | A→G | F58S (TTT→TCT) | glnP ← | L‑glutamine ABC transporter membrane subunit |
| RA | 848,854 | C→T | D20N (GAT→AAT) | dps ← | stationary phase nucleoid protein that sequesters iron and protects DNA from damage |
| RA | 868,414 | G→C | A299P (GCC→CCC) | gsiA → | glutathione ABC transporter ATP binding subunit GsiA |
| RA | 885,544 | A→G | L196L (CTT→CTC) | ybjI ← | 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase |
| RA | 889,923 | A→G | intergenic (‑104/‑166) | ybjL ← / → ybjM | putative transport protein YbjL/putative inner membrane protein |
| RA | 925,623 | A→G | intergenic (+83/+261) | clpA → / ← serW | ATP‑dependent Clp protease ATP‑binding subunit ClpA/tRNA‑Ser |
| RA | 932,340 | (A)8→7 | intergenic (‑290/‑255) | trxB ← / → lrp | thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp |
| RA | 935,179 | A→G | A652A (GCA→GCG) | ftsK → | cell division DNA translocase FtsK |
| RA | 964,250 | G→A | intergenic (+138/‑70) | ihfB → / → ycaI | integration host factor subunit beta/conserved inner membrane protein YcaI |
| RA | 1,000,908 | A→G | N565D (AAC→GAC) | elfC → | putative fimbrial usher protein ElfC |
| RA | 1,004,972 | Δ1 bp | coding (205/1011 nt) | pyrD → | dihydroorotate dehydrogenase, type 2 |
| RA | 1,010,898 | C→T | A312V (GCC→GTC) | uup → | ATP‑binding protein with possible role in replication |
| RA | 1,018,176 | G→A | L42L (CTG→TTG) | ycbZ ← | putative ATP‑dependent protease YcbZ |
| RA | 1,019,853 | G→C | N67K (AAC→AAG) | ompA ← | outer membrane porin A |
| RA | 1,029,953 | A→G | V6A (GTG→GCG) | rlmI ← | 23S rRNA m(5)C1962 methyltransferase |
| RA | 1,143,140 | T→C | T410A (ACT→GCT) | rne ← | ribonuclease E |
| RA | 1,159,950 | A→G | G534G (GGT→GGC) | fhuE ← | ferric coprogen/ferric rhodotorulic acid outer membrane transporter |
| RA | 1,160,848 | A→G | L235P (CTG→CCG) | fhuE ← | ferric coprogen/ferric rhodotorulic acid outer membrane transporter |
| RA | 1,161,362 | A→G | S64P (TCT→CCT) | fhuE ← | ferric coprogen/ferric rhodotorulic acid outer membrane transporter |
| RA | 1,183,673 | A→G | L258L (TTG→CTG) | potB ← | spermidine preferential ABC transporter membrane subunit PotB |
| RA | 1,191,945 | T→C | G14G (GGA→GGG) | purB ← | adenylosuccinate lyase |
| RA | 1,196,723 | A→G | intergenic (+350/+144) | icd → / ← ymfD | isocitrate dehydrogenase/e14 prophage; putative SAM‑dependent methyltransferase |
| RA | 1,220,971 | G→A | pseudogene (1371/2648 nt) | ycgH → | putative transporter component, N‑terminal fragment |
| RA | 1,257,583 | A→G | V77A (GTA→GCA) | ychF ← | redox‑responsive ATPase YchF |
| RA | 1,266,397 | G→A | A102T (GCA→ACA) | prmC → | protein‑(glutamine‑N(5)) methyltransferase |
| RA | 1,269,170 | A→G | *36Q (TAA→CAA) | ldrA ← | small toxic polypeptide LdrA |
| RA | 1,269,705 | A→G | *36Q (TAA→CAA) | ldrB ← | small toxic polypeptide LdrB |
| RA | 1,280,243 | C→T | A127V (GCG→GTG) | narG → | nitrate reductase A subunit alpha |
| RA | 1,280,714 | A→G | D284G (GAC→GGC) | narG → | nitrate reductase A subunit alpha |
| MC JC | 1,299,499 | Δ1,199 bp | insH21 | insH21 | |
| RA | 1,339,802 | C→T | A158V (GCC→GTC) | pgpB → | phosphatidylglycerophosphatase B |
| RA | 1,351,196 | (G)7→6 | intergenic (‑157/+211) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD |
| RA | 1,370,044 | (T)9→8 | intergenic (+41/‑172) | pspE → / → ycjM | thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase |
| RA | 1,377,092 | A→G | E87E (GAA→GAG) | ycjS → | putative oxidoreductase YcjS |
| RA | 1,390,763 | G→A | R34C (CGT→TGT) | ymjC ← | putative uncharacterized protein YmjC |
| RA | 1,419,486 | G→A | intergenic (‑30/+279) | ydaG ← / ← racR | Rac prophage; uncharacterized protein YdaG/Rac prophage; DNA‑binding transcriptional repressor RacR |
| JC JC | 1,470,429 | IS5 (+) +4 bp | coding (1072‑1075/1152 nt) | insI‑2 → | IS30 transposase |
| RA | 1,508,902 | A→G | S55P (TCG→CCG) | yncJ ← | protein YncJ |
| RA | 1,541,351 | C→T | E414K (GAA→AAA) | narZ ← | nitrate reductase Z subunit alpha |
| RA | 1,556,491 | T→C | intergenic (‑211/‑134) | bdm ← / → osmC | biofilm‑dependent modulation protein/osmotically inducible peroxiredoxin |
| RA | 1,594,256 | (C)8→9 | pseudogene (3831/5422 nt) | yneO ← | AIDA‑I family autotransporter YneO |
| RA | 1,624,072 | C→T | intergenic (‑222/+33) | dgcZ ← / ← ydeI | diguanylate cyclase DgcZ/BOF family protein YdeI |
| RA | 1,634,642 | C→T | G82S (GGC→AGC) | tfaQ ← | Qin prophage; putative tail fiber assembly protein TfaQ |
| RA | 1,682,766 | A→G | D203G (GAC→GGC) | rstA → | DNA‑binding transcriptional regulator RstA |
| RA | 1,685,542 | (C)6→5 | coding (1047/1404 nt) | fumC ← | fumarase C |
| RA | 1,714,949 | A→G | V191V (GTA→GTG) | gstA → | glutathione S‑transferase GstA |
| RA | 1,717,126 | G→A | P33S (CCG→TCG) | tyrS ← | tyrosine‑‑tRNA ligase |
| RA | 1,755,350 | T→C | intergenic (‑209/‑348) | fumD ← / → pykF | fumarase D/pyruvate kinase I |
| RA | 1,796,199 | T→C | A582A (GCA→GCG) | pheT ← | phenylalanine‑‑tRNA ligase subunit beta |
| RA | 1,873,384 | A→G | K448K (AAA→AAG) | dgcJ → | putative diguanylate cyclase DgcJ |
| JC JC | 1,879,829 | Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp | coding (115‑120/360 nt) | yeaR ← | DUF1971 domain‑containing protein YeaR |
| RA | 1,943,321 | T→C | R220R (CGT→CGC) | znuC → | Zn(2(+)) ABC transporter ATP binding subunit |
| MC JC | 1,978,503 | Δ776 bp | insB‑5–insA‑5 | insB‑5, insA‑5 | |
| RA | 2,002,039 | T→C | intergenic (‑250/+71) | fliA ← / ← fliC | RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein |
| RA | 2,007,555 | A→G | E467G (GAG→GGG) | amyA → | alpha‑amylase |
| RA | 2,034,473 | C→T | pseudogene (423/1193 nt) | yedS → | putative outer membrane protein YedS, N‑terminal fragment |
| RA | 2,078,942 | T→C | intergenic (+11/+90) | yoeF → / ← yeeX | CP4‑44 prophage; putative uncharacterized protein YoeF/DUF496 domain‑containing protein YeeX |
| RA | 2,090,754 | T→C | I188T (ATC→ACC) | hisG → | ATP phosphoribosyltransferase |
| RA | 2,111,535 | T→C | I148V (ATT→GTT) | rfbD ← | dTDP‑4‑dehydrorhamnose reductase |
| RA | 2,114,013 | G→A | G105G (GGC→GGT) | galF ← | UTP:glucose‑1‑phosphate uridylyltransferase, low activity |
| RA | 2,139,829 | T→C | N595S (AAT→AGT) | asmA ← | putative assembly protein AsmA |
| RA | 2,148,330 | T→C | G219G (GGT→GGC) | yegD → | HSP70 family protein YegD |
| RA | 2,151,145 | T→C | intergenic (‑160/‑40) | yegI ← / → yegJ | protein kinase YegI/DUF2314 domain‑containing protein YegJ |
| RA | 2,173,363 | Δ2 bp | pseudogene (915‑916/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
| RA | 2,190,478 | C→T | E145K (GAA→AAA) | yehB ← | putative fimbrial usher protein YehB |
| RA | 2,197,062 | T→C | L197L (TTA→CTA) | yehF → | putative protein YehF |
| RA | 2,210,908 | G→A | W603* (TGG→TGA) | yehQ → | SWIM zinc finger domains‑containing protein YehQ |
| RA | 2,227,037 | Δ1 bp | coding (234/762 nt) | yohF ← | putative oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| RA | 2,227,340 | (C)6→7 | pseudogene (1419/1436 nt) | mdtQ ← | putative multidrug resistance outer membrane protein MdtQ |
| RA | 2,259,392 | T→C | intergenic (‑96/+327) | psuK ← / ← fruA | putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA |
| RA | 2,269,260 | T→C | V136A (GTT→GCT) | lpxT → | Kdo2‑lipid A phosphotransferase |
| RA | 2,297,114 | (C)7→8 | coding (531/624 nt) | ccmA ← | heme trafficking system ATP‑binding protein |
| RA | 2,336,768 | T→C | intergenic (‑124/+25) | yfaA ← / ← gyrA | DUF2138 domain‑containing protein YfaA/DNA gyrase subunit A |
| RA | 2,339,206 | T→C | D72G (GAC→GGC) | gyrA ← | DNA gyrase subunit A |
| RA | 2,360,192 | A→G | intergenic (‑40/+17) | yfaV ← / ← rhmD | putative transporter YfaV/L‑rhamnonate dehydratase |
| RA | 2,416,753 | T→C | R669R (CGT→CGC) | pta → | phosphate acetyltransferase |
| RA | 2,437,568 | A→G | V95A (GTG→GCG) | pdxB ← | erythronate‑4‑phosphate dehydrogenase |
| RA | 2,446,579 | T→C | T299A (ACC→GCC) | aroC ← | chorismate synthase |
| RA | 2,450,334 | G→A | P86L (CCG→CTG) | yfcP ← | putative fimbrial protein YfcP |
| RA | 2,462,889 | (C)9→10 | intergenic (+243/‑123) | fadL → / → yfdF | long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF |
| RA | 2,492,417 | T→C | T280A (ACC→GCC) | frc ← | formyl‑CoA transferase |
| RA | 2,513,460 | T→C | V140A (GTC→GCC) | nupC → | nucleoside:H(+) symporter NupC |
| RA | 2,527,823 | T→C | V293A (GTG→GCG) | yfeH → | putative solute:Na(+) symporter |
| RA | 2,552,783 | T→C | G144G (GGT→GGC) | amiA → | N‑acetylmuramoyl‑L‑alanine amidase A |
| RA | 2,660,519 | A→G | V338A (GTG→GCG) | iscS ← | cysteine desulfurase |
| RA | 2,762,513 | A→G | V343A (GTC→GCC) | abpB ← | CP4‑57 prophage; putative helicase YfjK |
| RA | 2,811,134 | A→G | H122R (CAC→CGC) | mprA → | DNA‑binding transcriptional repressor MprA |
| MC JC | 2,857,096 | Δ2,538 bp | coding (4‑2541/2562 nt) | mutS → | DNA mismatch repair protein MutS |
| RA | 2,870,137 | T→C | T46T (ACA→ACG) | umpG ← | broad specificity 5'(3')‑nucleotidase and polyphosphatase |
| RA | 2,870,797 | T→C | S170G (AGC→GGC) | truD ← | tRNA pseudouridine(13) synthase |
| RA | 2,927,705 | T→C | intergenic (+33/‑524) | ppnN → / → sdaC | nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC |
| RA | 2,949,993 | (T)5→6 | coding (752/1332 nt) | argA → | N‑acetylglutamate synthase |
| RA | 2,962,491 | (C)5→6 | coding (274/324 nt) | ppdC ← | putative YgdA |
| RA | 2,982,448 | T→C | intergenic (‑266/+49) | araE ← / ← kduD | arabinose:H(+) symporter/putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase |
| RA | 2,988,500 | G→A | intergenic (+332/‑2) | yqeH → / → yqeI | putative LuxR family transcriptional regulator YqeH/putative transcriptional regulator YqeI |
| RA | 3,020,551 | A→T | Q4H (CAA→CAT) | ygfM → | putative oxidoreductase |
| RA | 3,068,157 | T→C | intergenic (‑77/+16) | yggE ← / ← argO | uncharacterized protein YggE/L‑arginine exporter |
| RA | 3,078,501 | (T)5→6 | coding (359/1389 nt) | cmtA ← | mannitol‑specific PTS enzyme IICB component CmtA |
| RA | 3,100,292 | T→C | D146G (GAC→GGC) | ansB ← | L‑asparaginase 2 |
| RA | 3,101,039 | (C)7→8 | coding (585/720 nt) | yggN ← | putative EcfF |
| RA | 3,163,684 | A→G | intergenic (‑203/+31) | plsC ← / ← parC | 1‑acylglycerol‑3‑phosphate O‑acyltransferase/DNA topoisomerase IV subunit A |
| RA | 3,187,989 | A→T | pseudogene (558/2434 nt) | yqiG → | putative outer membrane usher protein YqiG |
| RA | 3,233,352 | G→A | R563H (CGC→CAC) | fadH → | 2,4‑dienoyl‑CoA reductase |
| RA | 3,246,806 | C→T | A52V (GCC→GTC) | exuR → | DNA‑binding transcriptional repressor ExuR |
| RA | 3,261,403 | G→A | A339V (GCG→GTG) | tdcE ← | 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4 |
| RA | 3,270,488 | (C)7→8 | noncoding (105/377 nt) | rnpB ← | RNase P catalytic RNA component |
| RA | 3,280,942 | G→A | pseudogene (242/402 nt) | agaW → | N‑acetyl‑D‑galactosamine specific PTS truncated enzyme IIC component |
| RA | 3,354,487 | C→T | intergenic (‑437/‑238) | yhcC ← / → gltB | radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB |
| RA | 3,388,726 | A→G | V134A (GTT→GCT) | aaeA ← | aromatic carboxylic acid efflux pump membrane fusion protein |
| RA | 3,389,335 | C→T | M1M (ATG→ATA) † | aaeX ← | DUF1656 domain‑containing protein AaeX |
| RA | 3,443,320 | C→T | A260T (GCA→ACA) | secY ← | Sec translocon subunit SecY |
| RA | 3,541,362 | T→C | V319A (GTG→GCG) | feoB → | Fe(2(+)) transporter FeoB |
| RA | 3,557,086 | A→G | F332L (TTC→CTC) | rtcB ← | RNA‑splicing ligase |
| RA | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| RA | 3,616,067 | T→C | V276A (GTG→GCG) | nikB → | Ni(2(+)) ABC transporter membrane subunit NikB |
| RA | 3,754,800 | T→C | intergenic (‑372/+173) | yiaW ← / ← aldB | DUF3302 domain‑containing protein YiaW/aldehyde dehydrogenase B |
| RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
| RA | 3,860,630 | (C)5→4 | coding (348/894 nt) | yidL → | putative DNA‑binding transcriptional regulator YidL |
| RA | 3,893,082 | G→A | P106P (CCG→CCA) | yidZ → | putative LysR‑type transcriptional regulator YidZ |
| RA | 3,957,223 | G→A | P200S (CCG→TCG) | ilvY ← | DNA‑binding transcriptional dual regulator IlvY |
| RA | 4,048,215 | T→C | V417A (GTC→GCC) | polA → | DNA polymerase I |
| RA | 4,114,992 | A→G | C196C (TGT→TGC) | glpX ← | fructose‑1,6‑bisphosphatase 2 |
| RA | 4,117,855 | G→A | A79V (GCC→GTC) | glpF ← | glycerol facilitator |
| RA | 4,219,155 | A→G | D187G (GAC→GGC) | aceK → | isocitrate dehydrogenase kinase/phosphatase |
| RA | 4,271,009 | C→T | L118F (CTC→TTC) | yjbR → | PF04237 family protein YjbR |
| RA | 4,278,113 | A→G | intergenic (‑47/‑366) | ryjA ← / → ghxP | small RNA RyjA/guanine/hypoxanthine transporter GhxP |
| RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| RA | 4,298,452 | G→A | G305G (GGC→GGT) | fdhF ← | formate dehydrogenase H |
| RA | 4,322,037 | A→G | V129A (GTC→GCC) | phnF ← | putative transcriptional regulator PhnF |
| RA | 4,424,463 | (C)5→4 | intergenic (+77/+53) | ulaF → / ← yjfY | L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY |
| RA | 4,445,549 | A→G | T480A (ACC→GCC) | tamB → | translocation and assembly module subunit TamB |
| RA | 4,465,243 | T→C | Y313C (TAC→TGC) | treB ← | trehalose‑specific PTS enzyme IIBC component |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 226730–226968 | 228565–226988 | 21–1836 | 19 [18] | [18] 19 | rrlH | 23S ribosomal RNA |
| * | * | ÷ | NC_000913 | 2066538–2066706 | 2067184–2066726 | 21–647 | 19 [18] | [18] 21 | insH‑6 | CP4‑44 prophage; IS5 transposase and trans‑activator |
| * | * | ÷ | NC_000913 | 3423770–3424234 | 3424525–3424239 | 6–756 | 23 [18] | [12] 24 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | 1207790 = | 92 (1.100) | 26 (0.350) | 20/244 | 1.9 | 26.2% | coding (290/630 nt) | ycfK | e14 prophage; protein StfP |
| ? | NC_000913 | 1209619 = | 65 (0.880) | pseudogene (37/537 nt) | stfE | e14 prophage; putative side tail fiber protein fragment | |||||
| * | ? | NC_000913 | 3363001 = | 123 (1.470) | 138 (1.670) | 58/274 | 0.2 | 68.6% | coding (195/2382 nt) | yhcD | putative fimbrial usher protein YhcD |
| ? | NC_000913 | 3766087 = | 4 (0.050) | coding (3905/4134 nt) | rhsA | rhs element protein RhsA | |||||
| * | ? | NC_000913 | = 3653230 | NA (NA) | 83 (0.990) | 51/276 | 0.3 | 96.5% | noncoding (1/1195 nt) | IS5 | repeat region |
| ? | NC_000913 | = 3766089 | 3 (0.040) | coding (3907/4134 nt) | rhsA | rhs element protein RhsA | |||||