breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 6,892 A→G V356V (GTT→GTC)  yaaJ ← putative transporter YaaJ
RA 14,155 C→T intergenic (+76/‑13) dnaK → / → dnaJ chaperone protein DnaK/chaperone protein DnaJ
RA 42,683 +A coding (281/771 nt) fixA → putative electron transfer flavoprotein FixA
RA 48,757 T→C V330A (GTG→GCG)  kefC → K(+) : H(+) antiporter KefC
RA 70,787 T→C F134S (TTT→TCT)  araC → DNA‑binding transcriptional dual regulator AraC
RA 88,680 A→G N218S (AAC→AGC)  cra → DNA‑binding transcriptional dual regulator Cra
RA 101,917 A→G T385A (ACC→GCC)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 166,936 C→T A736V (GCC→GTC)  mrcB → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
RA 185,773 A→G L59L (TTG→CTG)  dapD ← tetrahydrodipicolinate succinylase
RA 205,802 A→G D226G (GAC→GGC)  dnaE → DNA polymerase III subunit alpha
RA 226,175 C→T noncoding (417/2904 nt) rrlH → 23S ribosomal RNA
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 269,495 C→T G230S (GGC→AGC)  perR ← putative transcriptional regulator PerR
RA 275,399 C→T G25G (GGC→GGT)  mmuP → S‑methyl‑L‑methionine transporter
RA 285,560 G→A D56N (GAC→AAC)  yagG → putative D‑xylonate transporter YagG
RA 297,941 T→C Q262Q (CAA→CAG)  paoD ← molybdenum cofactor insertion chaperone for PaoABC
RA 321,564 (G)7→8 intergenic (+483/‑44) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 359,221 A→G L141L (TTA→TTG)  cynT → carbonic anhydrase 1
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 404,225 T→C W175R (TGG→CGG)  dgcC → diguanylate cyclase DgcC
RA 422,549 (C)5→6 coding (32/1815 nt) malZ → maltodextrin glucosidase
RA 453,396 A→G G50G (GGT→GGC)  ampG ← muropeptide:H(+) symporter
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 539,933 A→G T263A (ACT→GCT)  allB → allantoinase
RA 563,278 C→T P315S (CCG→TCG)  sfmH → putative fimbrial adhesin protein SfmH
RA 573,138 G→A G19S (GGC→AGC)  ybcO → DLP12 prophage; putative nuclease YbcO
RA 621,912 C→T G93D (GGC→GAC)  fepD ← ferric enterobactin ABC transporter membrane subunit FebD
RA 645,421 A→G V387A (GTG→GCG)  citT ← citrate:succinate antiporter
RA 646,802 G→C G236G (GGC→GGG)  citG ← triphosphoribosyl‑dephospho‑CoA synthase
RA 648,307 A→G V422A (GTG→GCG)  citF ← citrate lyase alpha subunit
RA 669,834 (G)6→7 coding (74/612 nt) cobC ← putative adenosylcobalamin phosphatase/alpha‑ribazole phosphatase
RA 671,469 C→T G46E (GGA→GAA)  holA ← DNA polymerase III subunit delta
RA 693,180 A→G Y67H (TAT→CAT)  ybeZ ← PhoH‑like protein
RA 697,498 Δ1 bp intergenic (‑365/+15) metT ← / ← asnB tRNA‑Met/asparagine synthetase B
RA 701,613 A→G V380A (GTA→GCA)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 731,011 G→A A477T (GCC→ACC)  rhsC → rhs element protein RhsC
RA 731,472 T→G G630G (GGT→GGG)  rhsC → rhs element protein RhsC
RA 772,973 A→G T506A (ACC→GCC)  cydA → cytochrome bd‑I ubiquinol oxidase subunit I
RA 796,306 A→G I149V (ATT→GTT)  modB → molybdate ABC transporter membrane subunit
RA 810,576 (G)5→6 coding (196/1155 nt) bioF → 8‑amino‑7‑oxononanoate synthase
RA 811,441 C→T P354L (CCC→CTC)  bioF → 8‑amino‑7‑oxononanoate synthase
RA 829,819 (C)6→7 coding (154/999 nt) ybhG ← HlyD_D23 family protein YbhG
RA 834,704 G→A R545R (CGG→CGA)  dinG → ATP‑dependent DNA helicase DinG
RA 879,758 G→A G339G (GGG→GGA)  yliI → aldose sugar dehydrogenase YliI
RA 880,631 A→G intergenic (‑151/‑96) gstB ← / → dacC glutathione S‑transferase GstB/D‑alanyl‑D‑alanine carboxypeptidase DacC
RA 888,099 T→C intergenic (‑42/+35) rybB ← / ← ybjL small regulatory RNA RybB/putative transport protein YbjL
RA 889,799 T→C E7E (GAA→GAG)  ybjL ← putative transport protein YbjL
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 913,622 A→G F65L (TTC→CTC)  hcp ← protein S‑nitrosylase
RA 925,899 (G)7→8 noncoding (73/88 nt) serW ← tRNA‑Ser
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 963,206 A→G E404E (GAA→GAG)  rpsA → 30S ribosomal subunit protein S1
RA 986,679 Δ1 bp coding (304/1089 nt) ompF ← outer membrane porin F
RA 996,973 A→G P180P (CCT→CCC)  ssuE ← NADPH‑dependent FMN reductase
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,016,193 A→G F93S (TTC→TCC)  fabA ← beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase
RA 1,041,526 Δ1 bp coding (910/1299 nt) appA → periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase
RA 1,042,626 T→C T529A (ACG→GCG)  etk ← protein‑tyrosine kinase Etk
RA 1,101,784 A→G V115A (GTG→GCG)  csgF ← curli assembly component
RA 1,112,249 (G)6→7 coding (1387/2544 nt) opgH → osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
RA 1,116,136 A→G G149G (GGT→GGC)  lpxL ← lauroyl acyltransferase
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,150,644 C→T A306V (GCG→GTG)  fabD → [acyl‑carrier‑protein] S‑malonyltransferase
RA 1,167,461 T→C intergenic (+72/‑138) ndh → / → ycfJ NADH:quinone oxidoreductase II/PF05433 family protein YcfJ
RA 1,170,031 T→C Y115C (TAC→TGC)  ldtC ← L,D‑transpeptidase YcfS
RA 1,172,076 A→G V630A (GTT→GCT)  mfd ← transcription‑repair coupling factor
RA 1,181,512 T→C T72A (ACG→GCG)  ymfA ← putative inner membrane protein
RA 1,204,656 A→G T163A (ACC→GCC) 
pseudogene (256/1137 nt)
ymfN →
aaaE →
e14 prophage; chimeric replication protein/phage terminase YmfN
phage terminase
RA 1,211,762 C→T C81Y (TGT→TAT)  iraM ← anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,268,947 A→G E261E (GAA→GAG)  kdsA → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate synthase
RA 1,293,347 C→T intergenic (‑425/‑180) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,303,286 A→G T130A (ACC→GCC)  oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,322,975 (A)8→9 intergenic (‑29/+63) trpE ← / ← trpL anthranilate synthase subunit TrpE/trp operon leader peptide
RA 1,327,839 C→T T173T (ACG→ACA)  btuR ← cobinamide/cobalamin adenosyltransferase
RA 1,338,450 A→G T874A (ACC→GCC)  acnA → aconitate hydratase 1
RA 1,354,439 G→A A27V (GCC→GTC)  sapC ← putrescine ABC exporter membrane protein SapC
RA 1,357,038 A→G S25P (TCT→CCT)  sapA ← putative periplasmic binding protein SapA
RA 1,357,300 G→A intergenic (‑190/+123) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,403,605 C→T G321S (GGC→AGC)  abgA ← p‑aminobenzoyl‑glutamate hydrolase subunit A
RA 1,421,237 C→T A40V (GCA→GTA)  ydaU → Rac prophage; DUF1376 domain‑containing protein YdaU
RA 1,422,337 G→A A119T (GCA→ACA)  ydaV → Rac prophage; putative ATP‑binding protein YdaV
RA 1,448,665 T→C Y383H (TAT→CAT)  feaB → phenylacetaldehyde dehydrogenase
RA 1,459,364 C→T A104V (GCA→GTA)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase
RA 1,473,518 G→A pseudogene (3000/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,485,893 C→T R945C (CGC→TGC)  hrpA → ATP‑dependent RNA helicase HrpA
RA 1,517,665 A→G E6E (GAA→GAG)  ydcY → DUF2526 domain‑containing protein YdcY
RA 1,523,314 T→C L3S (TTA→TCA)  yncE → PQQ‑like domain‑containing protein YncE
RA 1,541,042 A→G Y517H (TAT→CAT)  narZ ← nitrate reductase Z subunit alpha
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,544,945 T→C N257S (AAC→AGC)  yddK ← leucine‑rich repeat domain‑containing protein YddK
RA 1,570,826 G→A P407L (CCG→CTG)  gadB ← glutamate decarboxylase B
RA 1,587,884 A→G F138S (TTT→TCT)  ydeR ← putative fimbrial protein YdeR
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,645,196 T→C K27E (AAA→GAA)  hokD ← Qin prophage; toxic protein HokD
RA 1,660,868 A→G I105V (ATC→GTC)  ynfF → putative selenate reductase YnfF
RA 1,677,388 A→G G172G (GGT→GGC)  pntA ← pyridine nucleotide transhydrogenase subunit alpha
RA 1,679,237 T→C Y271H (TAC→CAC)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,682,025 C→T G2G (GGG→GGA)  ydgC ← GlpM family protein
RA 1,684,919 G→A A221T (GCC→ACC)  tus → DNA replication terminus site‑binding protein
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,690,705 T→C V285A (GTA→GCA)  ydgA → conserved protein YdgA
RA 1,695,818 A→G V85A (GTG→GCG)  uidA ← beta‑glucuronidase
RA 1,723,568 T→C V483A (GTG→GCG)  ydhK → putative transporter YdhK
RA 1,772,172 T→C F368L (TTC→CTC)  ydiM → putative exporter YdiM
RA 1,783,669 T→C I231I (ATT→ATC)  fadK → short chain acyl‑CoA synthetase
RA 1,788,399 T→C intergenic (+121/‑36) ppsR → / → aroH posphoenolpyruvate synthetase regulatory protein/3‑deoxy‑7‑phosphoheptulonate synthase, Trp‑sensitive
RA 1,796,854 A→G V364A (GTT→GCT)  pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,824,706 T→C T78A (ACC→GCC)  ves ← HutD family protein Ves
RA 1,843,762 A→G intergenic (+48/+69) gdhA → / ← ynjI glutamate dehydrogenase/DUF1266 domain‑containing protein YnjI
RA 1,863,290 A→G T174A (ACC→GCC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,871,832 (G)6→5 coding (1448/1476 nt) cdgI → putative c‑di‑GMP binding protein CdgI
RA 1,873,384 A→G K448K (AAA→AAG)  dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,881,492 T→C G106G (GGA→GGG)  dmlR ← DNA‑binding transcriptional regulator DmlR
RA 1,886,730 G→A D296N (GAT→AAT)  yeaX → carnitine monooxygenase subunit YeaX
RA 1,898,605 T→C V60A (GTA→GCA)  pdeD → putative c‑di‑GMP phosphodiesterase PdeD
RA 1,920,932 G→A R237H (CGT→CAT)  rsmF → 16S rRNA m(5)C1407 methyltransferase
RA 1,928,983 T→C intergenic (‑144/+65) ptrB ← / ← yebE oligopeptidase B/conserved inner membrane protein YebE
RA 1,958,713 T→C H185R (CAC→CGC)  cutC ← protein CutC
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 1,996,183 T→C N217S (AAC→AGC)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 2,002,844 T→C T255A (ACA→GCA)  fliC ← flagellar filament structural protein
RA 2,008,870 T→C R198R (CGT→CGC)  yedE → putative selenium transporter YedE
RA 2,012,563 (G)6→7 pseudogene (62/279 nt) intG → putative defective phage integrase
RA 2,015,611 T→A D244E (GAT→GAA)  fliG → flagellar motor switch protein FliG
RA 2,017,280 C→T P243S (CCG→TCG)  fliI → flagellum‑specific ATP synthase FliI
RA 2,021,709 T→C V69A (GTG→GCG)  fliO → flagellar biosynthesis protein FliO
RA 2,055,057 A→G intergenic (+98/‑4) shiA → / → amn shikimate:H(+) symporter/AMP nucleosidase
RA 2,072,883 G→A A449T (GCG→ACG)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,073,251 G→A V571V (GTG→GTA)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,089,255 T→C T71A (ACC→GCC)  yoeB ← ribosome‑dependent mRNA interferase toxin YoeB
RA 2,110,445 G→A P192L (CCG→CTG)  rfbA ← dTDP‑glucose pyrophosphorylase
RA 2,134,865 G→A A263V (GCG→GTG)  wzc ← protein‑tyrosine kinase Wzc
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,175,780 G→A A181V (GCC→GTC)  gatZ ← tagatose‑1,6‑bisphosphate aldolase 2 subunit GatZ
RA 2,185,656 G→A G47G (GGC→GGT)  rcnR ← DNA‑binding transcriptional repressor RcnR
RA 2,198,685 A→G intergenic (+1387/‑1594) yehF → / → yehI putative protein YehF/DUF4132 domain‑containing protein YehI
RA 2,262,306 G→A C20C (TGC→TGT)  fruK ← 1‑phosphofructokinase
RA 2,281,013 A→G S128G (AGC→GGC)  radD → putative DNA repair helicase RadD
RA 2,302,942 G→A T24T (ACC→ACT)  napD ← NapA signal peptide‑binding chaperone NapD
RA 2,327,508 A→G G212G (GGT→GGC)  yfaP ← DUF2135 domain‑containing protein YfaP
RA 2,368,510 G→A A158T (GCT→ACT)  arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,395,029 T→C L6L (TTA→TTG)  nuoL ← NADH:quinone oxidoreductase subunit L
RA 2,399,538 T→C T210A (ACG→GCG)  nuoG ← NADH:quinone oxidoreductase subunit G
RA 2,410,707 T→C T216A (ACC→GCC)  yfbS ← putative transporter YfbS
RA 2,423,045 G→A D165D (GAC→GAT)  rpnB ← recombination‑promoting nuclease RpnB
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,460,937 T→C intergenic (‑3/‑369) yfcZ ← / → fadL DUF406 domain‑containing protein YfcZ/long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor
JC 2,462,889 (C)9→12 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,565,398 A→G intergenic (+66/+83) yffS → / ← eutA CPZ‑55 prophage; uncharacterized protein YffS/reactivating factor for ethanolamine ammonia‑lyase EutBC
RA 2,640,836 G→A G323G (GGC→GGT)  ispG ← (E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin)
RA 2,673,740 C→T P132L (CCG→CTG)  yphA → putative inner membrane protein
RA 2,680,452 G→A A765V (GCC→GTC)  yphG ← DUF5107 domain‑containing protein YphG
RA 2,705,117 C→T A65T (GCT→ACT)  lepB ← signal peptidase I
RA 2,709,225 C→T M60I (ATG→ATA)  rseA ← anti‑sigma factor
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,736,648 T→C V168A (GTA→GCA)  bamD → outer membrane protein assembly factor BamD
RA 2,746,240 T→C intergenic (‑55/+194) rpsP ← / ← ffh 30S ribosomal subunit protein S16/signal recognition particle protein component
RA 2,769,648 (C)6→5 intergenic (+162/‑55) yfjQ → / → yfjR CP4‑57 prophage; protein YfjQ/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,770,799 (C)5→4 coding (119/444 nt) yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,772,591 A→G pseudogene (592/1016 nt) yfjV ← CP4‑57 prophage; putative arsenite transporter, N‑terminal fragment
RA 2,772,999 G→A pseudogene (184/1016 nt) yfjV ← CP4‑57 prophage; putative arsenite transporter, N‑terminal fragment
RA 2,785,204 (A)8→7 intergenic (‑193/+558) ypjC ← / ← ileY DUF5507 domain‑containing protein YpjC/tRNA‑Ile
RA 2,792,180 A→G D303G (GAC→GGC)  gabD → NADP(+)‑dependent succinate‑semialdehyde dehydrogenase
RA 2,794,731 T→C V160A (GTC→GCC)  gabP → 4‑aminobutyrate:H(+) symporter
RA 2,798,530 (A)7→6 intergenic (‑35/‑634) stpA ← / → alaE DNA‑binding transcriptional repressor StpA with RNA chaperone activity/L‑alanine exporter
RA 2,804,419 G→A M306I (ATG→ATA)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,806,989 T→C V327A (GTT→GCT)  proW → glycine betaine ABC transporter membrane subunit ProW
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,827,717 A→G E119G (GAA→GGA)  srlB → sorbitol‑specific PTS enzyme IIA component
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,890,797 G→A P368S (CCG→TCG)  cysJ ← sulfite reductase, flavoprotein subunit
RA 2,920,941 C→T G387D (GGC→GAC)  gudP ← galactarate/glucarate/glycerate transporter GudP
RA 2,948,307 T→C Y235C (TAC→TGC)  amiC ← N‑acetylmuramoyl‑L‑alanine amidase C
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,960,227 A→G R734R (CGT→CGC)  recC ← exodeoxyribonuclease V subunit RecC
RA 2,973,022 T→C T281A (ACC→GCC)  lplT ← lysophospholipid transporter
RA 3,000,976 T→C V211A (GTT→GCT)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,002,032 T→C V563A (GTT→GCT)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,027,153 A→G A11A (GCA→GCG)  ghxQ → guanine/hypoxanthine transporter GhxQ
RA 3,028,806 (C)5→4 coding (207/489 nt) ygfS ← putative oxidoreductase, 4Fe‑4S ferredoxin‑type subunit
RA 3,041,435 G→A M41I (ATG→ATA)  ygfZ → folate‑binding protein
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,160,896 T→C D84G (GAC→GGC)  ygiQ ← radical SAM superfamily protein YgiQ
RA 3,171,047 G→A P188P (CCG→CCA)  qseC → sensory histidine kinase QseC
RA 3,181,417 A→G M333V (ATG→GTG)  ygiC → putative enzyme with ATPase activity
RA 3,191,075 T→C G148G (GGT→GGC)  yqiI → putative fimbrial protein YqiI
RA 3,201,476 C→T S90S (AGC→AGT)  ygiM → putative signal transduction protein (SH3 domain)
RA 3,207,365 C→T S301S (AGC→AGT)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,209,379 T→C G452G (GGT→GGC)  ttdT → L‑tartrate:succinate antiporter
RA 3,213,203 A→G I53V (ATT→GTT)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,245,898 T→C R225R (CGT→CGC)  exuT → hexuronate transporter
RA 3,304,813 (C)7→8 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,312,089 T→C D230G (GAT→GGT)  truB ← tRNA pseudouridine(55) synthase
RA 3,321,197 (T)7→6 coding (417/1626 nt) yhbX ← putative hydrolase, inner membrane
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,357,018 T→C L765P (CTG→CCG)  gltB → glutamate synthase subunit GltB
RA 3,358,292 A→G T1190A (ACT→GCT)  gltB → glutamate synthase subunit GltB
RA 3,372,310 C→T V89I (GTC→ATC)  nanT ← N‑acetylneuraminate:H(+) symporter
RA 3,375,895 (G)6→7 coding (1027/1368 nt) dcuD → putative transporter DcuD
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,406,593 C→T A226V (GCG→GTG)  accC → biotin carboxylase
RA 3,437,397 C→T H294Y (CAT→TAT)  trkA → NAD‑binding component of Trk potassium transporters
RA 3,455,545 A→G intergenic (‑147/‑33) gspA ← / → gspC Type II secretion system protein GspA/Type II secretion system protein GspC
RA 3,472,700 T→C N272S (AAC→AGC)  fusA ← elongation factor G
RA 3,490,338 A→G G164G (GGT→GGC)  pabA ← aminodeoxychorismate synthase subunit 2
RA 3,491,126 A→G V113A (GTT→GCT)  fic ← putative adenosine monophosphate‑‑protein transferase Fic
RA 3,552,586 A→G intergenic (‑113/‑499) malP ← / → malT maltodextrin phosphorylase/DNA‑binding transcriptional activator MalT
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,577,042 A→G V7A (GTT→GCT)  gntU ← low‑affinity gluconate transporter
RA 3,588,643 A→G L180L (TTG→CTG)  ugpC ← sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
RA 3,632,500 T→C K31E (AAA→GAA)  yhiJ ← DUF4049 domain‑containing protein YhiJ
RA 3,650,641 A→G E135E (GAA→GAG)  arsC → arsenate reductase
RA 3,650,680 (C)7→6 intergenic (+18/‑611) arsC → / → yhiS arsenate reductase/putative uncharacterized protein YhiS
RA 3,660,338 A→G A369A (GCA→GCG)  mdtE → multidrug efflux pump membrane fusion protein MdtE
RA 3,669,830 A→G D80G (GAC→GGC)  treF → cytoplasmic trehalase
RA 3,732,867 A→G T223A (ACG→GCG)  xylG → xylose ABC transporter ATP binding subunit
RA 3,748,407 A→G T442A (ACC→GCC)  lyxK → L‑xylulose kinase
RA 3,787,890 T→C G351G (GGT→GGC)  envC → murein hydrolase activator EnvC
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,822,554 T→C V52A (GTT→GCT)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,822,657 A→G E86E (GAA→GAG)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,852,535 T→C S84G (AGC→GGC)  ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,862,224 G→A V83V (GTC→GTT)  glvB ← putative PTS enzyme II component GlvB
RA 3,879,326 T→C N265S (AAC→AGC)  gyrB ← DNA gyrase subunit B
RA 3,919,120 G→A G246G (GGC→GGT)  atpA ← ATP synthase F1 complex subunit alpha
RA 3,926,512 A→G intergenic (‑57/+33) mioC ← / ← asnC flavoprotein MioC/DNA‑binding transcriptional dual regulator AsnC
RA 3,948,080 T→C intergenic (‑113/‑6) hdfR ← / → maoP DNA‑binding transcriptional dual regulator HdfR/macrodomain Ori protein
RA 3,999,973 T→C V664A (GTC→GCC)  uvrD → ssDNA translocase and dsDNA helicase ‑ DNA helicase II
RA 4,030,743 A→G F77L (TTC→CTC)  fadB ← dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
RA 4,037,211 C→T noncoding (71/77 nt) ileT → tRNA‑Ile
RA 4,050,105 A→G intergenic (+98/+28) spf → / ← yihA small regulatory RNA Spot 42/GTP‑binding protein YihA
RA 4,070,029 G→A P125S (CCG→TCG)  yihR ← putative aldose 1‑epimerase YihR
RA 4,132,292 T→C intergenic (+25/‑324) metL → / → metF fused aspartate kinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase
RA 4,160,400 C→T D131N (GAC→AAC)  sthA ← soluble pyridine nucleotide transhydrogenase
RA 4,250,294 A→G A247A (GCA→GCG)  malM → maltose regulon periplasmic protein
RA 4,254,975 G→A P498S (CCG→TCG)  plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,321,237 T→C E4G (GAA→GGA)  phnH ← carbon‑phosphorus lyase core complex subunit PhnH
RA 4,328,984 C→T S617L (TCG→TTG)  crfC → clamp‑binding sister replication fork colocalization protein
RA 4,394,057 G→A P4L (CCC→CTC)  queG ← epoxyqueuosine reductase
RA 4,443,799 A→G D473G (GAC→GGC)  tamA → translocation and assembly module subunit TamA
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,472,426 (A)8→9 coding (108/135 nt) pyrL ← pyrBI operon leader peptide
RA 4,546,391 G→A G432G (GGG→GGA)  fimD → type I fimbriae usher protein
RA 4,565,618 C→T C78Y (TGC→TAC)  yjiM ← putative dehydratase subunit
RA 4,567,589 A→G W311R (TGG→CGG)  mdtM ← multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 4,568,116 C→T G135E (GGG→GAG)  mdtM ← multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 4,583,950 T→G N938H (AAC→CAC)  hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,589,124 C→T intergenic (‑45/+5) yjiX ← / ← btsT conserved protein YjiX/pyruvate:H(+) symporter
RA 4,589,872 C→T A470T (GCG→ACG)  btsT ← pyruvate:H(+) symporter
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,330 (G)8→7 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,637,743 (A)9→8 coding (1048/1425 nt) creC → sensory histidine kinase CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 736858–736904 737382–737309 406–525 18 [17] [17] 18 [ybfL] [ybfL]
* * ÷ NC_000913 3423764–3424234 3424525–3424238 5–762 18 [17] [12] 21 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3762347–3764072 3765220–3764077 6–2874 18 [17] [17] 18 rhsA rhs element protein RhsA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =82 (1.110)24 (0.370) 18/244 2.1 26.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 62 (0.950)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)68 (0.950) 50/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =138 (1.860)115 (1.560) 59/274 0.1 62.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)75 (1.010) 44/276 0.4 93.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA