breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 7,240 A→G S240S (AGT→AGC)  yaaJ ← putative transporter YaaJ
RA 18,179 (G)5→4 coding (691/1167 nt) nhaA → Na(+):H(+) antiporter NhaA
RA 25,107 A→G H906R (CAC→CGC)  ileS → isoleucine‑‑tRNA ligase
RA 49,008 A→G T414A (ACC→GCC)  kefC → K(+) : H(+) antiporter KefC
RA 55,555 A→G Y519H (TAT→CAT)  lptD ← lipopolysaccharide assembly protein LptD
RA 93,294 T→C G43G (GGT→GGC)  murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 141,108 A→G S40P (TCC→CCC)  gcd ← quinoprotein glucose dehydrogenase
RA 195,355 A→G I151M (ATA→ATG)  ispU → undecaprenyl diphosphate synthase
RA 204,483 A→G E379G (GAG→GGG)  lpxB → lipid A disaccharide synthase
RA 210,131 +G coding (453/2142 nt) ldcC → lysine decarboxylase 2
RA 217,044 A→G intergenic (‑41/+13) yaeF ← / ← proS peptidase C92 family protein/proline‑‑tRNA ligase
RA 243,501 C→T intergenic (‑198/‑42) fadE ← / → lpcA acyl‑CoA dehydrogenase/D‑sedoheptulose 7‑phosphate isomerase
RA 257,015 T→C R163R (CGT→CGC)  frsA → fermentation‑respiration switch protein
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 289,044 A→G L40P (CTC→CCC)  xynR ← CP4‑6 prophage; DNA‑binding transcriptional repressor XynR
RA 308,771 +C coding (562/2526 nt) ecpC ← putative fimbrial usher protein EcpC
RA 369,543 T→C G311G (GGT→GGC)  mhpA → putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
RA 375,247 A→G intergenic (+366/‑212) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 375,269 A→G intergenic (+388/‑190) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 375,277 G→A intergenic (+396/‑182) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 391,703 (G)6→7 pseudogene (1453/1458 nt) yaiT → putative autotransporter YaiT
RA 395,434 G→A T285M (ACG→ATG)  ampH ← peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
RA 397,197 Δ1 bp coding (559/1221 nt) sbmA → peptide antibiotic/peptide nucleic acid transporter
RA 428,237 T→C H197H (CAT→CAC)  secD → Sec translocon accessory complex subunit SecD
RA 435,614 (T)6→7 intergenic (+58/‑20) nusB → / → thiL transcription antitermination protein NusB/thiamine monophosphate kinase
RA 503,225 +G coding (14/1677 nt) ybaL ← putative transporter YbaL
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 544,082 Δ1 bp coding (1211/1236 nt) allC ← allantoate amidohydrolase
RA 595,675 C→T R26C (CGT→TGT)  cusC → copper/silver export system outer membrane channel
RA 601,164 T→A N817K (AAT→AAA)  cusA → copper/silver export system RND permease
RA 609,948 A→G R44R (CGT→CGC)  entD ← phosphopantetheinyl transferase EntD
RA 616,008 A→G T618A (ACG→GCG)  entF → apo‑serine activating enzyme
RA 634,194 A→G E203E (GAA→GAG)  ybdL → methionine‑oxo‑acid transaminase, PLP‑dependent
RA 712,538 A→G S89P (TCC→CCC)  ybfF ← esterase
RA 796,504 A→G I215V (ATT→GTT)  modB → molybdate ABC transporter membrane subunit
RA 799,693 T→C T295A (ACT→GCT)  ybhD ← putative DNA‑binding transcriptional regulator YbhD
RA 815,306 T→G V594G (GTC→GGC)  uvrB → excision nuclease subunit B
RA 828,749 A→G L78P (CTG→CCG)  ybhF ← putative ABC exporter ATP binding subunit
RA 880,671 A→G intergenic (‑191/‑56) gstB ← / → dacC glutathione S‑transferase GstB/D‑alanyl‑D‑alanine carboxypeptidase DacC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 917,719 A→G Q416R (CAG→CGG)  ybjD → DUF2813 domain‑containing protein YbjD
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 940,462 A→G V345V (GTA→GTG)  serS → serine‑‑tRNA ligase
RA 971,326 A→G T159A (ACC→GCC)  kdsB → 3‑deoxy‑manno‑octulosonate cytidylyltransferase
RA 973,349 A→G L18P (CTG→CCG)  elyC ← envelope biogenesis factor
RA 979,986 C→T R1221C (CGT→TGT)  mukB → chromosome partitioning protein MukB
RA 1,004,161 A→G E72G (GAA→GGA)  ycbF → putative fimbrial chaperone YcbF
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,049,478 A→G G89G (GGT→GGC)  gfcA ← threonine‑rich inner membrane protein GfcA
RA 1,067,692 A→G V6A (GTG→GCG)  wrbA ← NAD(P)H:quinone oxidoreductase
RA 1,070,255 A→G F34L (TTC→CTC)  rutF ← flavin reductase
RA 1,079,601 G→A V99V (GTG→GTA)  putP → proline:Na(+) symporter
RA 1,093,346 (T)6→7 coding (471/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,147,993 A→G I125V (ATT→GTT)  plsX → putative phosphate acyltransferase
RA 1,162,592 (G)5→6 coding (346/378 nt) ycfL → DUF1425 domain‑containing protein YcfL
RA 1,167,286 A→G Y401C (TAC→TGC)  ndh → NADH:quinone oxidoreductase II
RA 1,205,638 T→G G30G (GGT→GGG)  ymfR → e14 prophage; protein YmfR
RA 1,206,043 A→G T105A (ACC→GCC)  beeE → e14 prophage; putative protein BeeE
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,222,786 C→T G68D (GGT→GAT)  ymgG ← PF13488 family protein YmgG
RA 1,245,511 A→G T118A (ACC→GCC)  ycgY → uncharacterized protein YcgY
RA 1,294,524 C→T pseudogene (799/897 nt) insZ ← putative transposase InsZ
RA 1,303,637 T→C S247P (TCT→CCT)  oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,304,427 G→T A198A (GCG→GCT)  oppC → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppC
RA 1,370,708 A→G S165G (AGC→GGC)  ycjM → glucosylglycerate phosphorylase
RA 1,404,074 A→G L164L (CTT→CTC)  abgA ← p‑aminobenzoyl‑glutamate hydrolase subunit A
RA 1,421,650 (C)7→6 coding (532/858 nt) ydaU → Rac prophage; DUF1376 domain‑containing protein YdaU
RA 1,437,648 A→G L1046P (CTG→CCG)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,445,005 G→A G652D (GGC→GAC)  ydbH → PF11739 family protein YdbH
RA 1,493,384 A→G E305E (GAA→GAG)  trg → methyl‑accepting chemotaxis protein Trg
RA 1,535,229 A→C G210G (GGT→GGG)  yddE ← PF02567 family protein YddE
RA 1,535,877 G→A intergenic (‑19/+60) yddE ← / ← narV PF02567 family protein YddE/nitrate reductase Z subunit gamma
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,570,741 T→C E435E (GAA→GAG)  gadB ← glutamate decarboxylase B
RA 1,576,260 A→G Y454H (TAT→CAT)  yddB ← putative TonB‑dependent receptor
RA 1,592,043 A→G L45P (CTG→CCG)  hipA ← serine/threonine kinase HipA
RA 1,594,131 (C)8→6 pseudogene (3955‑3956/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,594,256 (C)8→9 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
JC 1,597,313 (C)7→10 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,614,688 C→A A6S (GCA→TCA)  sad ← NAD(+)‑dependent succinate semialdehyde dehydrogenase
RA 1,620,752 A→G V129A (GTG→GCG)  eamA ← cysteine/O‑acetylserine exporter EamA
RA 1,624,188 G→A P104S (CCG→TCG)  ydeI ← BOF family protein YdeI
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,651,822 T→C pseudogene (311/1197 nt) intQ → Qin prophage; putative defective integrase
RA 1,674,655 (C)6→7 coding (743/1035 nt) tqsA → autoinducer 2 exporter
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,687,513 A→G F289L (TTT→CTT)  fumA ← fumarase A
RA 1,713,392 T→C G208G (GGT→GGC)  dtpA → dipeptide/tripeptide:H(+) symporter DtpA
RA 1,718,598 G→A A335A (GCC→GCT)  anmK ← anhydro‑N‑acetylmuramic acid kinase
RA 1,735,789 T→C S138P (TCA→CCA)  sodB → superoxide dismutase (Fe)
RA 1,756,145 G→A G150S (GGT→AGT)  pykF → pyruvate kinase I
RA 1,786,943 T→C N57S (AAC→AGC)  ppsA ← phosphoenolpyruvate synthetase
RA 1,788,844 T→C V137A (GTC→GCC)  aroH → 3‑deoxy‑7‑phosphoheptulonate synthase, Trp‑sensitive
RA 1,797,729 A→T D72E (GAT→GAA)  pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,823,934 (C)5→4 coding (420/888 nt) cho → endonuclease of nucleotide excision repair
RA 1,832,698 A→G I91V (ATT→GTT)  xthA → exodeoxyribonuclease III
RA 1,847,995 G→T S5* (TCG→TAG)  selD ← selenide, water dikinase
RA 1,849,394 T→C G186G (GGT→GGC)  sppA → protease IV, a signal peptide peptidase
RA 1,850,034 G→A V400I (GTT→ATT)  sppA → protease IV, a signal peptide peptidase
RA 1,858,333 T→C K167K (AAA→AAG)  ydjJ ← putative zinc‑binding dehydrogenase YdjJ
RA 1,859,352 G→A T293I (ACC→ATC)  ydjK ← putative transporter YdjK
RA 1,868,282 T→C F459L (TTC→CTC)  yeaG → protein kinase YeaG
RA 1,873,384 A→G K448K (AAA→AAG)  dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,885,377 T→C L238P (CTG→CCG)  yeaW → carnitine monooxygenase subunit YeaW
RA 1,888,441 T→C T436A (ACC→GCC)  fadD ← fatty acyl‑CoA synthetase
RA 1,892,288 T→C E316E (GAA→GAG)  yoaA ← putative 5' to 3' DNA helicase implicated in DNA repair
RA 1,899,034 T→C V203A (GTA→GCA)  pdeD → putative c‑di‑GMP phosphodiesterase PdeD
RA 1,923,469 T→C T80A (ACC→GCC)  yebY ← DUF2511 domain‑containing protein YebY
RA 1,936,414 T→C intergenic (‑100/‑238) zwf ← / → yebK NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase/DNA‑binding transcriptional repressor YebK
RA 1,975,233 T→C Q31R (CAG→CGG)  cheA ← chemotaxis protein CheA
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,001,935 +G intergenic (‑146/+175) fliA ← / ← fliC RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
RA 2,030,962 (T)5→6 coding (1356/1419 nt) dcm ← DNA‑cytosine methyltransferase
RA 2,033,994 (T)8→7 intergenic (+255/‑57) rseX → / → yedS small regulatory RNA RseX/putative outer membrane protein YedS, N‑terminal fragment
MC 2,067,354 Δ35,590 bp between IS5 yoeA–insH‑7 41 genes
RA 2,114,707 G→A S397L (TCA→TTA)  wcaM ← putative colanic acid biosynthesis protein WcaM
RA 2,116,513 G→A G205G (GGC→GGT)  wcaL ← putative colanic biosynthesis glycosyl transferase
RA 2,117,829 T→C K192K (AAA→AAG)  wcaK ← putative colanic acid biosynthesis pyruvyl transferase
RA 2,137,049 T→C P65P (CCA→CCG)  wza ← outer membrane polysaccharide export protein Wza
RA 2,144,943 A→G T560A (ACG→GCG)  dgcE → putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,187,068 T→C Y37H (TAT→CAT)  rcnB → periplasmic protein involved in nickel/cobalt export
RA 2,213,464 A→G V394A (GTG→GCG)  btsS ← high‑affinity pyruvate receptor
RA 2,224,154 G→C G239G (GGC→GGG)  pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,233,515 A→G H213R (CAC→CGC)  sanA → DUF218 domain‑containing protein SanA
RA 2,262,962 (C)5→6 coding (534/1131 nt) fruB ← fructose‑specific PTS multiphosphoryl transfer protein FruB
RA 2,267,665 T→C V474A (GTA→GCA)  yeiQ → putative dehydrogenase, NAD‑dependent
RA 2,285,722 C→T A449A (GCC→GCT)  yejM → putative cardiolipin transport protein
RA 2,304,988 T→C intergenic (+595/+120) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,305,013 A→G intergenic (+620/+95) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,305,027 G→A intergenic (+634/+81) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,312,373 T→C D126G (GAC→GGC)  ompC ← outer membrane porin C
RA 2,339,176 T→C D82G (GAC→GGC)  gyrA ← DNA gyrase subunit A
RA 2,350,215 G→A A266V (GCC→GTC)  glpQ ← glycerophosphoryl diester phosphodiesterase
RA 2,369,665 G→A G543S (GGT→AGT)  arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,386,665 A→G H269R (CAC→CGC)  yfbL → putative peptidase YfbL
RA 2,396,080 T→C E124G (GAA→GGA)  nuoI ← NADH:quinone oxidoreductase subunit I
RA 2,397,693 A→G S825P (TCA→CCA)  nuoG ← NADH:quinone oxidoreductase subunit G
RA 2,405,651 (C)5→6 intergenic (‑579/+52) nuoA ← / ← lrhA NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA
RA 2,437,681 T→C K57K (AAA→AAG)  pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,442,175 T→C C138R (TGC→CGC)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,496,504 A→G Y287C (TAC→TGC)  lpxP → palmitoleoyl acyltransferase
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,615,271 T→C E204G (GAG→GGG)  yfgO ← putative transporter YfgO
RA 2,648,364 T→C V1308V (GTA→GTG)  yfhM ← alpha2‑macroglobulin
RA 2,663,259 G→A A22V (GCC→GTC)  trmJ ← tRNA Cm32/Um32 methyltransferase
RA 2,667,775 T→C L24L (CTA→CTG)  hcaT ← putative 3‑phenylpropionate transporter
RA 2,675,097 T→C D222G (GAT→GGT)  yphC ← putative zinc‑binding dehydrogenase YphC
RA 2,710,553 C→T S45L (TCA→TTA)  nadB → L‑aspartate oxidase
RA 2,718,291 T→C T80A (ACG→GCG)  yfiF ← putative methyltransferase
RA 2,742,347 T→C V158A (GTC→GCC)  yfiR → DUF4154 domain‑containing protein YfiR
RA 2,742,729 T→C F116S (TTC→TCC)  dgcN → diguanylate cyclase DgcN
RA 2,743,395 T→C V338A (GTA→GCA)  dgcN → diguanylate cyclase DgcN
RA 2,757,662 (T)8→9 coding (939/957 nt) yfjH ← CP4‑57 prophage; protein YfjH
RA 2,764,948 Δ1 bp coding (206/1617 nt) abpA ← CP4‑57 prophage; anti‑bacteriophage protein
RA 2,765,414 A→G intergenic (‑261/+99) abpA ← / ← yfjM CP4‑57 prophage; anti‑bacteriophage protein/CP4‑57 prophage; protein YfjM
RA 2,778,930 (C)6→7 coding (3797/4581 nt) ypjA ← adhesin‑like autotransporter YpjA
RA 2,805,736 C→T R308C (CGC→TGC)  proV → glycine betaine ABC transporter ATP binding subunit ProV
RA 2,809,002 A→G pseudogene (687/1178 nt) ygaY → putative transporter YgaY, N‑terminal fragment
RA 2,810,197 A→G Q194R (CAG→CGG)  ygaZ → L‑valine exporter subunit YgaZ
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,817,834 T→C noncoding (27/77 nt) argQ ← tRNA‑Arg
RA 2,818,784 T→C intergenic (‑139/+177) serV ← / ← csrA tRNA‑Ser/carbon storage regulator
RA 2,821,137 G→A A292V (GCG→GTG)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,835,963 T→C Q488R (CAG→CGG)  hypF ← carbamoyltransferase HypF
RA 2,837,711 T→C D132G (GAC→GGC)  hydN ← putative electron transport protein HydN
RA 2,839,090 T→C T59A (ACT→GCT)  ascG ← DNA‑binding transcriptional repressor AscG
RA 2,844,085 G→A R42R (CGC→CGT)  hycG ← formate hydrogenlyase subunit HycG
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,891,910 T→C intergenic (‑12/‑304) cysJ ← / → queD sulfite reductase, flavoprotein subunit/6‑carboxy‑5,6,7,8‑tetrahydropterin synthase
RA 2,900,874 T→C Y95H (TAT→CAT)  yqcE → putative transport protein YqcE
RA 2,902,963 G→A T356T (ACG→ACA)  ygcE → putative sugar kinase YgcE
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,990,434 A→C intergenic (‑74/‑120) yqeK ← / → ygeF protein YqeK/protein YgeF
RA 2,997,971 T→C pseudogene (18/300 nt) ygeP ← protein YgeP
RA 2,999,755 (C)6→7 coding (137/756 nt) ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,003,479 (G)6→7 coding (866/879 nt) xdhB → putative xanthine dehydrogenase FAD‑binding subunit XdhB
RA 3,060,772 (T)8→9 intergenic (+126/‑78) argP → / → scpA DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
MC JC 3,131,341 Δ191 bp IS5‑mediated yghQ ← putative transport protein YghQ
RA 3,162,717 T→C intergenic (‑48/+27) ftsP ← / ← plsC cell division protein required during stress conditions/1‑acylglycerol‑3‑phosphate O‑acyltransferase
RA 3,177,509 (C)6→7 coding (402/630 nt) nudF ← ADP‑sugar pyrophosphatase
RA 3,200,205 A→G V254A (GTG→GCG)  ygiF ← inorganic triphosphatase
RA 3,231,441 A→G intergenic (+202/‑224) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase
RA 3,240,406 T→C W155R (TGG→CGG)  sstT → serine/threonine:Na(+) symporter
RA 3,240,984 (G)7→8 coding (1041/1245 nt) sstT → serine/threonine:Na(+) symporter
RA 3,250,078 T→C R132R (CGT→CGC) 
M1A (GTG→GCG) †
yqjE →
yqjK →
conserved inner membrane protein YqjE
conserved protein YqjK
RA 3,257,649 A→G V121A (GTA→GCA)  cyuP ← putative D/L‑serine transporter
RA 3,275,916 G→A G212D (GGC→GAC)  garD → galactarate dehydratase
RA 3,304,212 G→A G255G (GGG→GGA)  yhbW → putative luciferase‑like monooxygenase
RA 3,304,813 (C)7→8 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,319,940 G→A G435D (GGC→GAC)  argG → argininosuccinate synthetase
RA 3,321,393 T→C D74G (GAC→GGC)  yhbX ← putative hydrolase, inner membrane
RA 3,337,816 T→C N26D (AAT→GAT)  mlaC ← intermembrane phospholipid transport system ‑ periplasmic binding protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,361,696 A→G H174R (CAC→CGC)  gltF → periplasmic protein GltF
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,423,458 C→T noncoding (85/120 nt) rrfF ← 5S ribosomal RNA
RA 3,448,477 T→C H31R (CAC→CGC)  rpsQ ← 30S ribosomal subunit protein S17
RA 3,475,447 G→A T91M (ACG→ATG)  tusD ← sulfurtransferase complex subunit TusD
RA 3,484,970 T→C W161R (TGG→CGG)  prkB → putative phosphoribulokinase
RA 3,486,448 A→C N110T (AAC→ACC)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,531,110 G→A Q444* (CAA→TAA)  yhgE ← putative transport protein YhgE
RA 3,534,231 A→G T472A (ACC→GCC)  pck → phosphoenolpyruvate carboxykinase (ATP)
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,561,621 C→T G68D (GGC→GAC)  glpE ← thiosulfate sulfurtransferase GlpE
RA 3,662,411 A→G L666L (TTA→TTG)  mdtF → multidrug efflux pump RND permease MdtF
RA 3,692,258 T→C Y117C (TAC→TGC)  bcsB ← cellulose synthase periplasmic subunit
RA 3,708,091 T→C intergenic (‑386/+525) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,214 G→A V35V (GTC→GTT)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,747,700 A→G H206R (CAC→CGC)  lyxK → L‑xylulose kinase
RA 3,772,577 T→C G99G (GGT→GGC)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,777,538 T→C V47A (GTG→GCG)  lldP → lactate/glycolate:H(+) symporter LldP
RA 3,788,824 C→T Q242* (CAG→TAG)  yibQ → divergent polysaccharide deacetylase domain‑containing protein YibQ
RA 3,802,925 Δ1 bp coding (134/1020 nt) waaO ← UDP‑D‑glucose:(glucosyl)LPS alpha‑1,3‑glucosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,831,149 T→C R231R (CGT→CGC)  yicH → AsmA famly protein YicH
RA 3,844,073 T→C V37A (GTC→GCC)  adeD → adenine deaminase
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,882,822 A→G V303A (GTG→GCG)  dnaA ← chromosomal replication initiator protein DnaA
RA 3,905,125 A→G I158T (ATC→ACC)  bglF ← beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
RA 3,908,180 A→G Y63H (TAC→CAC)  pstB ← phosphate ABC transporter ATP binding subunit
RA 3,911,408 C→G V40L (GTG→CTG)  pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,931,183 (G)7→8 intergenic (‑90/‑133) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup
RA 3,955,352 T→C S8P (TCC→CCC)  ilvA → threonine deaminase
RA 3,967,388 T→C G324G (GGT→GGC)  rho → transcription termination factor Rho
RA 3,982,684 (C)8→9 noncoding (79/87 nt) leuT → tRNA‑Leu
RA 4,020,242 T→C V6A (GTA→GCA)  ubiB → ubiquinone biosynthesis protein UbiB
RA 4,065,386 T→C G127G (GGA→GGG)  yihO ← putative sulfoquinovose transporter
RA 4,105,746 (C)5→6 intergenic (‑76/‑74) cpxR ← / → cpxP DNA‑binding transcriptional dual regulator CpxR/periplasmic protein CpxP
RA 4,200,155 (C)6→5 intergenic (+61/‑126) yjaG → / → hupA conserved protein YjaG/DNA‑binding protein HU‑alpha
RA 4,206,123 A→G G470G (GGT→GGC)  purH ← bifunctional AICAR transformylase/IMP cyclohydrolase
RA 4,223,381 G→A A83V (GCA→GTA)  iclR ← DNA‑binding transcriptional repressor IclR
RA 4,231,763 (A)7→6 intergenic (‑132/+121) yjbD ← / ← lysC conserved protein YjbD/aspartate kinase III
RA 4,235,842 A→G intergenic (+435/‑64) pgi → / → yjbE glucose‑6‑phosphate isomerase/uncharacterized protein YjbE
RA 4,258,235 T→C V165A (GTA→GCA)  dinF → DNA damage‑inducible protein F
RA 4,264,913 A→G P200P (CCA→CCG)  dnaB → replicative DNA helicase
RA 4,271,898 G→A A658A (GCC→GCT)  uvrA ← excision nuclease subunit A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,300,614 C→T G139G (GGG→GGA)  mdtP ← putative multidrug efflux pump outer membrane channel
RA 4,357,799 A→G G273G (GGT→GGC)  cadA ← lysine decarboxylase 1
RA 4,406,165 (T)6→5 intergenic (+180/‑25) purA → / → nsrR adenylosuccinate synthetase/DNA‑binding transcriptional repressor NsrR
RA 4,413,140 A→G Y38C (TAC→TGC)  yjfC → putative acid‑‑amine ligase YjfC
RA 4,435,385 T→A D394V (GAT→GTT)  cpdB ← 2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
RA 4,442,353 +G intergenic (‑177/‑29) msrA ← / → tamA methionine sulfoxide reductase A/translocation and assembly module subunit TamA
RA 4,443,061 A→G N227S (AAC→AGC)  tamA → translocation and assembly module subunit TamA
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,534,210 T→C intergenic (‑157/+220) topAI ← / ← yjhZ KpLE2 phage‑like element; toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/KpLE2 phage‑like element; putative acetyltransferase YjhZ
RA 4,534,223 A→G intergenic (‑170/+207) topAI ← / ← yjhZ KpLE2 phage‑like element; toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/KpLE2 phage‑like element; putative acetyltransferase YjhZ
RA 4,545,573 A→T I160F (ATC→TTC)  fimD → type I fimbriae usher protein
RA 4,556,769 (T)9→10 coding (116/144 nt) ytiD → protein YtiD
RA 4,556,939 T→C G9G (GGT→GGC)  idlP → iraD leader peptide
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 580696 581288–581123 428–593 45 [41] [41] 42 [nohD] [nohD]
* * ÷ NC_000913 3423770–3424234 3424526–3424238 5–757 43 [34] [37] 42 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =64 (0.450)106 (0.840) 60/244 0.5 64.1% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 62 (0.490)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780564 (0.450)77 (0.610) 52/244 0.8 56.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 62 (0.490)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)151 (1.090) 72/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =239 (1.670)246 (1.740) 94/274 0.1 67.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)203 (1.420) 90/276 0.1 97.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 410172118 (0.130)39 (0.290)
+GGGGGG
28/250 2.8 68.8% coding (32/984 nt) kdgT 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
?NC_000913 4101722 = 18 (0.130)coding (33/984 nt) kdgT 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter