breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 39,219 G→T intergenic (‑104/+25) caiB ← / ← caiA gamma‑butyrobetainyl‑CoA:carnitine CoA transferase/crotonobetainyl‑CoA reductase
RA 209,615 G→T intergenic (+35/‑64) accA → / → ldcC acetyl‑CoA carboxyltransferase subunit alpha/lysine decarboxylase 2
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 344,676 C→T Y167Y (TAC→TAT)  yahL → uncharacterized protein YahL
RA 605,177 A→G V83A (GTG→GCG)  nfsB ← NAD(P)H nitroreductase NfsB
RA 689,676 C→A P402P (CCG→CCT)  lnt ← apolipoprotein N‑acyltransferase
RA 844,373 G→T L370M (CTG→ATG)  ybiO ← moderate conductance mechanosensitive channel
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,042,437 C→A A592S (GCA→TCA)  etk ← protein‑tyrosine kinase Etk
RA 1,462,806 C→A R228S (CGT→AGT)  paaK → phenylacetate‑CoA ligase
RA 1,571,925 C→T V41I (GTC→ATC)  gadB ← glutamate decarboxylase B
RA 1,586,888 G→T Q283K (CAA→AAA)  ydeQ ← putative fimbrial adhesin protein YdeQ
RA 1,594,707 G→T pseudogene (3380/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,738,025 C→A S61Y (TCT→TAT)  purR → DNA‑binding transcriptional repressor PurR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,890,435 C→A S33I (AGC→ATC)  yeaY ← Slp family lipoprotein YeaY
RA 1,906,222 G→T V179F (GTC→TTC)  mntP → Mn(2(+)) exporter
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,286,657 G→T pseudogene (2258/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,307,351 G→T P422Q (CCG→CAG)  yojI ← ABC transporter family protein/microcin J25 efflux protein
RA 2,477,621 G→T P3P (CCC→CCA)  dsdC ← DNA‑binding transcriptional dual regulator DsdC
RA 2,521,499 C→A intergenic (+171/+94) lysV → / ← xapR tRNA‑Lys/DNA‑binding transcriptional activator XapR
RA 2,615,136 G→T S249* (TCG→TAG)  yfgO ← putative transporter YfgO
RA 2,886,193 C→A A343S (GCC→TCC)  cas3 ← CRISPR‑associated endonuclease/helicase Cas3
RA 3,044,990 C→A G443G (GGC→GGA)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,216,092 C→A V134L (GTG→TTG)  nfeF ← NADPH‑dependent ferric chelate reductase
RA 3,317,324 A→G V68A (GTC→GCC)  nusA ← transcription termination/antitermination protein NusA
RA 3,331,197 C→A V186V (GTG→GTT)  obgE ← GTPase ObgE
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,397,022 C→A E258D (GAG→GAT)  rng ← RNase G
RA 3,488,966 C→A G405G (GGG→GGT)  argD ← N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,565,112 G→T G490G (GGC→GGA)  glgP ← glycogen phosphorylase
RA 3,617,537 G→T A175S (GCA→TCA)  nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD
RA 3,734,742 G→T V341V (GTG→GTT)  xylH → xylose ABC transporter membrane subunit
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,926,823 G→T P61T (CCG→ACG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 4,224,158 G→T A111S (GCG→TCG)  metH → cobalamin‑dependent methionine synthase
RA 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,352,911 G→T A2S (GCA→TCA)  ghoT → toxin of the GhoTS toxin‑antitoxin system
RA 4,357,514 C→A L368L (CTG→CTT)  cadA ← lysine decarboxylase 1
RA 4,576,834 G→T pseudogene (2700/2721 nt) yjiV → putative uncharacterized protein YjiV

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102972 3104055 1084 32 [31] [30] 32 [mutY] [mutY]
* * ÷ NC_000913 3423761–3424233 3424540–3424239 7–780 32 [31] [31] 33 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =128 (1.140)40 (0.400) 33/246 1.3 28.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 86 (0.870)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)141 (1.300) 75/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =194 (1.730)155 (1.400) 74/276 0.1 61.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 6 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)151 (1.350) 65/278 0.2 96.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA