breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 11,843 A→G intergenic (‑57/‑320) yaaI ← / → dnaK DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
RA 162,800 G→T L232L (CTG→CTT)  hrpB → putative ATP‑dependent RNA helicase HrpB
RA 216,895 C→A A37S (GCC→TCC)  yaeF ← peptidase C92 family protein
RA 248,885 C→A pseudogene (1186/1713 nt) lfhA ← flagellar system protein, promoterless fragment
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 260,977 C→A A197E (GCA→GAA)  proB → glutamate 5‑kinase
RA 273,037 G→T R64I (AGA→ATA)  ykfC → CP4‑6 prophage; PF00078 domain‑containing protein YkfC
RA 345,634 C→G I77M (ATC→ATG)  yahM → uncharacterized protein YahM
RA 438,373 G→T A602D (GCC→GAC)  dxs ← 1‑deoxy‑D‑xylulose‑5‑phosphate synthase
RA 657,527 C→A intergenic (+26/+28) cspE → / ← crcB transcription antiterminator and regulator of RNA stability/F(‑) channel
RA 730,713 T→C S377S (AGT→AGC)  rhsC → rhs element protein RhsC
RA 743,686 C→A R32R (CGA→AGA)  pxpB → 5‑oxoprolinase component B
RA 781,266 G→T intergenic (+44/‑103) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 804,254 G→T S251I (AGT→ATT)  ybhJ → putative hydratase YbhJ
RA 863,883 A→G D81G (GAT→GGT)  fsaA → fructose‑6‑phosphate aldolase 1
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,232,026 G→T E281* (GAG→TAG)  umuC → DNA polymerase V catalytic protein
RA 1,241,617 G→T S152* (TCG→TAG)  cvrA ← putative K(+):H(+) antiporter
RA 1,270,590 A→G intergenic (+130/+159) rdlC → / ← chaA small regulatory antisense RNA RdlC/Na(+)/K(+):H(+) antiporter ChaA
RA 1,461,985 C→A R365S (CGC→AGC)  paaJ → beta‑ketoadipyl‑CoA thiolase
RA 1,525,848 C→A G45C (GGC→TGC)  ansP ← L‑asparagine transporter
RA 1,531,968 G→A G51G (GGG→GGA)  ydcC → H repeat‑associated putative transposase YdcC
RA 1,672,355 G→T G132G (GGG→GGT)  ydgD → putative serine protease YdgD
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,772,181 Δ1 bp coding (1111/1215 nt) ydiM → putative exporter YdiM
RA 1,849,590 G→T E252* (GAG→TAG)  sppA → protease IV, a signal peptide peptidase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,908,123 T→C intergenic (‑532/+138) yobF ← / ← yebO DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,112,528 G→T V178V (GTC→GTA)  rfbB ← dTDP‑glucose 4,6‑dehydratase 1
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,264,955 G→T E365* (GAG→TAG)  setB → sugar exporter SetB
RA 2,456,005 G→T intergenic (‑359/+322) yfcV ← / ← sixA putative fimbrial protein YfcV/phosphohistidine phosphatase
RA 2,501,727 C→A R200S (CGC→AGC)  ypdC → putative AraC‑type DNA‑binding transcriptional regulator YpdC
RA 2,541,193 G→T R163S (CGC→AGC)  cysW ← sulfate/thiosulfate ABC transporter inner membrane subunit CysW
RA 2,564,803 C→A P94Q (CCG→CAG)  yffS → CPZ‑55 prophage; uncharacterized protein YffS
RA 2,566,516 C→A G123G (GGG→GGT)  eutA ← reactivating factor for ethanolamine ammonia‑lyase EutBC
RA 2,735,495 C→A A173S (GCG→TCG)  rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase
RA 2,829,646 C→A R200R (CGC→CGA)  srlR → DNA‑binding transcriptional repressor SrlR
RA 2,994,412 C→A pseudogene (474/503 nt) pbl → transglycosylase domain‑containing protein Pbl
RA 3,036,812 G→T A432E (GCG→GAG)  recJ ← ssDNA‑specific exonuclease RecJ
RA 3,176,911 C→A D124Y (GAC→TAC)  yqiB ← DUF1249 domain‑containing protein YqiB
RA 3,279,496 G→T D195Y (GAT→TAT)  kbaZ → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
RA 3,297,739 C→A A134S (GCG→TCG)  yraQ ← permease family protein YraQ
RA 3,311,942 C→A G279V (GGA→GTA)  truB ← tRNA pseudouridine(55) synthase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,395,380 C→A P293P (CCG→CCT)  yhdP ← AsmA2 domain‑containing protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,786,393 G→T E378D (GAG→GAT)  gpmM → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,994,856 G→T R32L (CGC→CTC)  dapF → diaminopimelate epimerase
RA 4,121,732 G→T P6T (CCA→ACA)  hslU ← ATPase component of the HslVU protease
RA 4,125,587 G→T I408I (ATC→ATA)  priA ← primosome factor N'
RA 4,228,109 G→T A127S (GCC→TCC)  yjbB → putative inorganic phosphate export protein
RA 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A
RA 4,269,433 C→A A7E (GCA→GAA)  aphA → acid phosphatase/phosphotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,357,030 G→T P530T (CCG→ACG)  cadA ← lysine decarboxylase 1
RA 4,413,619 G→T G198W (GGG→TGG)  yjfC → putative acid‑‑amine ligase YjfC
RA 4,491,339 G→T P396H (CCT→CAT)  idnT ← L‑idonate/5‑ketogluconate/gluconate transporter
RA 4,509,183 C→A pseudogene (175/471 nt) insO → KpLE2 phage‑like element; IS911B regulator fragment
RA 4,574,648 G→T pseudogene (514/2721 nt) yjiV → putative uncharacterized protein YjiV

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104055 1086 27 [22] [25] 27 [mutY] [mutY]
* * ÷ NC_000913 3423767–3424234 3424525–3424238 5–759 26 [25] [20] 27 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =12 (0.130)134 (1.610) 60/246 0.1 90.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 19 (0.230)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780513 (0.140)79 (0.950) 50/246 0.3 83.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 19 (0.230)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)110 (1.210) 62/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =151 (1.610)129 (1.380) 60/276 0.2 62.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 6 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)116 (1.230) 59/278 0.2 92.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.100)coding (3907/4134 nt) rhsA rhs element protein RhsA