breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 9,558 C→A P85T (CCC→ACC)  mog → molybdopterin adenylyltransferase
RA 23,199 C→T A270V (GCC→GTC)  ileS → isoleucine‑‑tRNA ligase
RA 91,791 C→A R127S (CGC→AGC)  ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 99,715 G→T A24A (GCG→GCT)  murG → N‑acetylglucosaminyl transferase
RA 123,007 G→T intergenic (+151/‑10) pdhR → / → aceE DNA‑binding transcriptional dual regulator PdhR/pyruvate dehydrogenase E1 component
RA 173,699 G→A P395L (CCG→CTG)  hemL ← glutamate‑1‑semialdehyde aminotransferase
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 371,618 G→T W127L (TGG→TTG)  mhpC → 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
RA 603,786 G→T P293H (CCT→CAT)  ybdG ← miniconductance mechanosensitive channel YbdG
RA 634,400 C→A P272Q (CCG→CAG)  ybdL → methionine‑oxo‑acid transaminase, PLP‑dependent
RA 636,335 G→T L79M (CTG→ATG)  ybdN ← putative PAPS reductase/DUF3440 domain‑containing protein YbdN
RA 668,620 G→T P32P (CCC→CCA)  rlmH ← 23S rRNA m(3)psi1915 methyltransferase
RA 731,731 C→A R717R (CGG→AGG)  rhsC → rhs element protein RhsC
RA 751,168 G→T P334P (CCC→CCA)  ybgQ ← putative fimbrial usher protein YbgQ
RA 867,888 G→T V123V (GTG→GTT)  gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 871,236 G→T E90D (GAG→GAT)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 922,231 G→T G629C (GGC→TGC)  macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 938,930 G→C V313L (GTG→CTG)  rarA → recombination factor
RA 1,105,202 C→A P81Q (CCG→CAG)  csgC → inhibitor of CsgA amyloid formation
RA 1,196,220 C→T H366H (CAC→CAT)  icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT)  icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,429,994 G→T A316S (GCC→TCC)  stfR → Rac prophage; putative membrane protein
RA 1,533,572 G→T R94S (CGT→AGT)  yddH ← flavin reductase‑like protein YddH
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,686,461 G→T S43* (TCA→TAA)  fumC ← fumarase C
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,941,820 G→C A255G (GCT→GGT)  znuA ← Zn(2(+)) ABC transporter periplasmic binding protein
RA 1,943,179 G→C G173A (GGC→GCC)  znuC → Zn(2(+)) ABC transporter ATP binding subunit
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,003,389 G→T S73Y (TCC→TAC)  fliC ← flagellar filament structural protein
RA 2,138,644 G→T R248L (CGG→CTG)  yegH → inner membrane protein YegH
RA 2,173,361 +C pseudogene (918/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC 2,173,362 Δ3 bp pseudogene (915‑917/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,176,687 G→T A173E (GCG→GAG)  gatY ← tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
RA 2,357,917 C→A pseudogene (102/186 nt) ypaA → protein YpaA
RA 2,686,972 G→A V380M (GTG→ATG)  hmp → nitric oxide dioxygenase
RA 2,770,738 G→T A20S (GCC→TCC)  yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,905,112 G→T P103T (CCA→ACA)  queE ← putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,972,063 C→A S156* (TCG→TAG)  tas → putative NADP(H)‑dependent aldo‑keto reductase Tas
RA 3,017,295 2 bp→CT coding (1236‑1237/3099 nt) ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,083,215 C→A G195C (GGC→TGC)  speB ← agmatinase
RA 3,160,189 G→T P320T (CCA→ACA)  ygiQ ← radical SAM superfamily protein YgiQ
RA 3,169,742 G→A intergenic (‑66/‑86) ygiW ← / → qseB BOF family protein YgiW/DNA‑binding transcriptional activator QseB
RA 3,185,169 C→A intergenic (+39/‑245) ubiK → / → ygiL ubiquinone biosynthesis accessory factor UbiK/putative fimbrial protein YgiL
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,559,527 G→T T420T (ACG→ACT)  rtcR → DNA‑binding transcriptional activator RtcR
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,857,480 G→C P309A (CCG→GCG)  yidJ ← putative sulfatase/phosphatase
RA 3,870,554 C→A G393W (GGG→TGG)  dgoT ← putative D‑galactonate transporter
RA 4,054,314 G→T A322E (GCG→GAG)  glnG ← DNA‑binding transcriptional dual regulator NtrC
RA 4,059,732 G→T V442V (GTG→GTT)  bipA → ribosome‑dependent GTPase, ribosome assembly factor
RA 4,223,261 G→T A123E (GCG→GAG)  iclR ← DNA‑binding transcriptional repressor IclR
RA 4,228,109 G→T A127S (GCC→TCC)  yjbB → putative inorganic phosphate export protein
RA 4,231,855 C→A intergenic (‑224/+29) yjbD ← / ← lysC conserved protein YjbD/aspartate kinase III
RA 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,311,497 G→T F182L (TTC→TTA)  alsB ← D‑allose ABC transporter periplasmic binding protein
RA 4,500,378 C→A pseudogene (414/1199 nt) yjgX ← KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196277 1211437 15161 47 [43] [39] 47 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102949 3104069 1121 46 [43] [43] 44 mutY mutY
* * ÷ NC_000913 3423764–3424234 3424538–3424238 5–775 44 [43] [43] 47 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3619335–3621083 3622194–3621086 4–2860 47 [42] [43] 44 rhsB rhs element protein RhsB

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)198 (1.370) 88/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =268 (1.800)215 (1.450) 85/276 0.1 61.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 7 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)179 (1.200) 91/278 0.1 95.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA