breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 7,144 C→A E272D (GAG→GAT)  yaaJ ← putative transporter YaaJ
RA 28,272 G→T intergenic (+65/‑102) rihC → / → dapB ribonucleoside hydrolase RihC/4‑hydroxy‑tetrahydrodipicolinate reductase
RA 32,266 C→A L484M (CTG→ATG)  carB → carbamoyl phosphate synthetase subunit beta
RA 189,303 G→T S68S (TCC→TCA)  map ← methionine aminopeptidase
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 559,046 G→T G25* (GGA→TGA)  sfmC → putative fimbrial chaperone SfmC
RA 580,273 G→T S58* (TCA→TAA)  ylcI ← DLP12 prophage; DUF3950 domain‑containing protein YlcI
RA 622,331 G→T S11I (AGC→ATC)  entS → enterobactin exporter EntS
RA 760,657 C→A A651E (GCG→GAG)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 849,358 G→T A247E (GCG→GAG)  rhtA ← L‑threonine/L‑homoserine exporter
RA 868,758 G→T S413S (TCG→TCT)  gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,058,640 G→T S186I (AGC→ATC)  torC → cytochrome c menaquinol dehydrogenase TorC
RA 1,447,224 G→T C20* (TGC→TGA)  feaR ← DNA‑binding transcriptional activator FeaR
JC JC 1,621,212 IS5 (+) +4 bp intergenic (‑75/‑117) eamA ← / → ydeE cysteine/O‑acetylserine exporter EamA/dipeptide exporter
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,920,117 G→C E870Q (GAA→CAA)  yebT → intermembrane transport protein YebT
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 1,984,502 G→T R190R (CGA→AGA)  araG ← arabinose ABC transporter ATP binding subunit
RA 2,079,749 G→T S281R (AGC→AGA)  yeeA ← putative transporter YeeA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,204,907 C→A A104A (GCC→GCA)  yehL → putative AAA(+) MoxR family ATPase YehL
RA 2,488,746 Δ1 bp coding (423/1146 nt) yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,575,228 G→T P77Q (CCG→CAG)  eutP ← putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain
RA 2,870,483 G→T F274L (TTC→TTA)  truD ← tRNA pseudouridine(13) synthase
RA 2,941,694 C→A A292S (GCC→TCC)  gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 3,058,923 C→A V135F (GTT→TTT)  rpiA ← ribose‑5‑phosphate isomerase A
RA 3,113,188 G→T P97Q (CCG→CAG)  yghG ← lipoprotein YghG
RA 3,352,017 C→A D337Y (GAT→TAT)  arcB ← sensory histidine kinase ArcB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,414,676 C→A G271G (GGC→GGA)  acrE → multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,480,378 G→A P77L (CCC→CTC)  kefB ← K(+) : H(+) antiporter KefB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,873,800 G→T L225L (CTC→CTA)  dgoK ← 2‑dehydro‑3‑deoxygalactonokinase
RA 3,908,515 G→T intergenic (‑149/+34) pstB ← / ← pstA phosphate ABC transporter ATP binding subunit/phosphate ABC transporter membrane subunit PstA
RA 4,124,617 C→A G732C (GGT→TGT)  priA ← primosome factor N'
RA 4,125,373 C→A G480C (GGT→TGT)  priA ← primosome factor N'
RA 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,434,110 G→T intergenic (‑86/‑3) qorB ← / → ytfH NAD(P)H:quinone oxidoreductase/putative transcriptional regulator YtfH
RA 4,449,960 C→A intergenic (‑308/‑2) ppa ← / → ytfQ inorganic pyrophosphatase/galactofuranose ABC transporter periplasmic binding protein
RA 4,539,099 C→A A135S (GCT→TCT)  nanC ← N‑acetylneuraminic acid outer membrane channel
RA 4,581,769 G→A T427M (ACG→ATG)  hsdM ← type I restriction enzyme EcoKI methyltransferase component

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1640952 1641043 92 18 [17] [17] 18 essQ/cspB Qin prophage; putative S lysis protein/Qin prophage; cold shock‑like protein CspB
* * ÷ NC_000913 2470136 2470505 370 18 [16] [17] 21 [yfdI] [yfdI]
* * ÷ NC_000913 3102993 3104069 1077 18 [17] [17] 19 mutY mutY
* * ÷ NC_000913 3423785–3424234 3424530–3424242 9–746 20 [14] [17] 19 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3499351 3499459 109 18 [16] [17] 18 [yhfL] [yhfL]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =62 (0.780)32 (0.450) 22/246 1.5 41.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 36 (0.510)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780563 (0.790)28 (0.400) 24/246 1.3 37.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 36 (0.510)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)36 (0.470) 29/270 1.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =74 (0.930)77 (0.970) 44/276 0.4 67.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)79 (0.990) 50/278 0.3 95.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA