breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 21,379 G→T G67* (GGA→TGA)  yaaY → DUF2575 domain‑containing protein YaaY
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 288,809 G→T G118G (GGC→GGA)  xynR ← CP4‑6 prophage; DNA‑binding transcriptional repressor XynR
RA 711,559 C→A intergenic (‑94/+46) fldA ← / ← ybfE flavodoxin 1/LexA‑regulated protein
RA 751,704 C→A V156F (GTC→TTC)  ybgQ ← putative fimbrial usher protein YbgQ
RA 794,407 G→T R80S (CGC→AGC)  modE ← DNA‑binding transcriptional dual regulator ModE
RA 810,479 G→T W33C (TGG→TGT)  bioF → 8‑amino‑7‑oxononanoate synthase
RA 845,414 Δ1 bp coding (67/2226 nt) ybiO ← moderate conductance mechanosensitive channel
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,100,596 C→A L101I (CTT→ATT)  ycdZ → putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT)  icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT)  icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,369,736 C→A P16P (CCC→CCA)  pspE → thiosulfate sulfurtransferase PspE
RA 1,457,108 A→C T227P (ACC→CCC)  paaE → phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit
RA 1,479,173 (T)7→6 coding (36/1758 nt) ynbC → hydrolase/methyltransferase domain‑containing protein YnbC
RA 1,663,870 G→T A88S (GCT→TCT)  ynfH → putative menaquinol dehydrogenase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,736,811 C→T M160I (ATG→ATA)  ydhP ← putative transporter YdhP
RA 1,774,957 C→A A91E (GCG→GAG)  aroD → 3‑dehydroquinate dehydratase
RA 1,831,689 G→T R98R (CGC→CGA)  astC ← succinylornithine transaminase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,029,494 T→C intergenic (‑128/‑45) yedI ← / → yedA conserved inner membrane protein YedI/putative transporter YedA
RA 2,072,113 C→A A192E (GCG→GAG)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,226,878 C→A Q131H (CAG→CAT)  yohF ← putative oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 2,518,263 C→A intergenic (‑32/‑204) alaW ← / → yfeC tRNA‑Ala/putative DNA‑binding transcriptional regulator YfeC
RA 2,625,040 G→A G666D (GGC→GAC)  ppk → polyphosphate kinase
RA 2,630,555 C→A A77E (GCG→GAG)  yfgI → nalidixic acid resistance protein YfgI
RA 2,790,155 C→A P58P (CCC→CCA)  lhgO → L‑2‑hydroxyglutarate oxidase
RA 2,841,419 G→T E144* (GAA→TAA)  ascB → 6‑phospho‑beta‑glucosidase AscB
RA 2,902,528 G→T P211P (CCG→CCT)  ygcE → putative sugar kinase YgcE
RA 2,920,701 G→T P16T (CCG→ACG)  gudX ← glucarate dehydratase‑related protein
RA 2,961,072 C→A A453S (GCG→TCG)  recC ← exodeoxyribonuclease V subunit RecC
RA 2,963,965 C→A E71D (GAG→GAT)  ppdA ← conserved protein PpdA
RA 3,052,193 G→T A42E (GCG→GAG)  ubiI ← 2‑octaprenylphenol hydroxylase
RA 3,202,877 G→T W329C (TGG→TGT)  cca → fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,554,444 C→A L454M (CTG→ATG)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,215,356 Δ1 bp intergenic (+147/‑122) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,252,926 C→A R141R (CGA→AGA)  ubiC → chorismate lyase
RA 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,337,342 G→T T196T (ACC→ACA)  adiY ← DNA‑binding transcriptional activator AdiY
RA 4,382,176 G→T A48D (GCT→GAT)  frdA ← fumarate reductase flavoprotein subunit
RA 4,399,968 G→T L239F (TTG→TTT)  miaA → tRNA dimethylallyltransferase
RA 4,406,938 C→A G95G (GGC→GGA)  rnr → RNase R
RA 4,640,034 G→T L91L (CTC→CTA)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196277 1211431 15155 16 [14] [13] 15 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102973 3104076 1104 15 [14] [14] 15 mutY mutY
* * ÷ NC_000913 3423766–3424234 3424523–3424239 6–758 16 [14] [9] 15 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3941746–3942013 3943292–3942038 26–1547 15 [13] [14] 15 [rrsC] [rrsC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)89 (1.470) 54/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =103 (1.650)93 (1.500) 55/276 0.1 64.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)65 (1.040) 41/278 0.3 98.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA