breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 127,167 G→T K491N (AAG→AAT)  aceF → pyruvate dehydrogenase, E2 subunit
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 312,789 G→A intergenic (‑12/+352) ykgM ← / ← ykgR putative ribosomal protein/putative membrane protein YkgR
RA 434,100 C→A Q216K (CAG→AAG)  ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase
RA 524,094 C→A F278L (TTC→TTA)  rhsD → protein RhsD
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 979,183 C→A A953D (GCC→GAC)  mukB → chromosome partitioning protein MukB
RA 1,075,591 G→T P1098T (CCG→ACG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,181,365 C→A G121W (GGG→TGG)  ymfA ← putative inner membrane protein
RA 1,260,443 G→T L121M (CTG→ATG)  dauA ← aerobic C4‑dicarboxylate transporter DauA
RA 1,305,652 C→A P300Q (CCG→CAG)  oppD → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppD
RA 1,467,182 G→T pseudogene (1791/2518 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,575,419 C→A W734L (TGG→TTG)  yddB ← putative TonB‑dependent receptor
JC JC 1,623,963 IS1 (+) +9 bp intergenic (‑113/+134) dgcZ ← / ← ydeI diguanylate cyclase DgcZ/BOF family protein YdeI
RA 1,642,413 G→T intergenic (+202/+76) ynfS → / ← ydfT Qin prophage; protein YnfS/Qin prophage; putative antitermination protein Q
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,725,682 C→A *80L (TGA→TTA)  ydhL ← conserved protein YdhL
RA 1,859,965 T→C N89D (AAT→GAT)  ydjK ← putative transporter YdjK
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,025,858 G→T T116T (ACG→ACT)  yedP → putative mannosyl‑3‑phosphoglycerate phosphatase
RA 2,168,867 G→T E52D (GAG→GAT)  yegS → lipid kinase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
JC JC 2,273,195 IS1 (+) +8 bp coding (832‑839/1815 nt) yejA → putative oligopeptide ABC transporter periplasmic binding protein
RA 2,403,820 G→T P9H (CCC→CAC)  nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,414,112 G→T G215C (GGT→TGT)  ackA → acetate kinase
RA 2,566,086 C→A E267* (GAA→TAA)  eutA ← reactivating factor for ethanolamine ammonia‑lyase EutBC
RA 2,569,681 C→A G219G (GGG→GGT)  eutJ ← putative chaperonin, ethanolamine utilization protein
RA 2,578,259 G→T S40* (TCA→TAA)  maeB ← malate dehydrogenase
RA 2,602,199 C→A L128M (CTG→ATG)  hyfB → hydrogenase 4 component B
RA 2,610,050 G→T D522Y (GAC→TAC)  hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,818,989 G→T A53D (GCT→GAT)  csrA ← carbon storage regulator
RA 2,831,224 G→T P356T (CCG→ACG)  norR ← DNA‑binding transcriptional dual regulator NorR
RA 2,940,438 C→A S269I (AGC→ATC)  rlmM ← 23S rRNA 2'‑O‑ribose C2498 methyltransferase
RA 3,002,178 C→A L612M (CTG→ATG)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,121,435 G→T intergenic (‑156/+199) glcA ← / ← glcB glycolate/lactate:H(+) symporter GlcA/malate synthase G
RA 3,203,636 G→T L166M (CTG→ATG)  bacA ← undecaprenyl pyrophosphate phosphatase
RA 3,242,301 C→A P338P (CCG→CCT)  uxaA ← D‑altronate dehydratase
RA 3,247,284 G→T K211N (AAG→AAT)  exuR → DNA‑binding transcriptional repressor ExuR
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,410,940 C→A L221M (CTG→ATG)  dusB → tRNA‑dihydrouridine synthase B
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,833,060 C→A S493I (AGC→ATC)  yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,891,534 C→T intergenic (+51/‑81) tnaB → / → mdtL tryptophan:H(+) symporter TnaB/efflux pump MdtL
RA 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A
RA 4,287,594 C→A intergenic (‑223/‑170) acs ← / → nrfA acetyl‑CoA synthetase (AMP‑forming)/cytochrome c552 nitrite reductase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,319,756 G→T A303E (GCG→GAG)  phnI ← carbon‑phosphorus lyase core complex subunit PhnI
RA 4,412,487 G→T R34L (CGC→CTC)  yjfM → DUF1190 domain‑containing protein YjfM
RA 4,440,035 G→T P394Q (CCG→CAG)  ytfL ← putative inner membrane protein
RA 4,500,309 C→A pseudogene (483/1199 nt) yjgX ← KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
RA 4,617,038 G→T Q10K (CAA→AAA)  yjjI ← DUF3029 domain‑containing protein YjjI
RA 4,625,193 C→A P100Q (CCG→CAG)  serB → phosphoserine phosphatase
RA 4,639,167 G→T G330G (GGG→GGT)  creD → putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102973 3104052 1080 40 [38] [37] 40 [mutY] [mutY]
* * ÷ NC_000913 3423761–3424234 3424531–3424238 5–771 40 [38] [37] 39 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =30 (0.230)177 (1.520) 72/246 0.1 89.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 14 (0.120)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780530 (0.230)131 (1.120) 60/246 0.3 86.6% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 14 (0.120)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)168 (1.310) 85/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =225 (1.710)181 (1.380) 81/276 0.1 61.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)118 (0.900) 62/278 0.5 92.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA