breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 138,793 C→A intergenic (+160/+42) cueO → / ← gcd cuprous oxidase/multicopper oxidase with role in copper homeostasis/quinoprotein glucose dehydrogenase
MC JC 257,908 Δ776 bp insB9–[crl] insB9, insA9, [crl]
RA 283,803 G→T V201V (GTG→GTT)  yagF → CP4‑6 prophage; D‑xylonate dehydratase
RA 361,130 G→T W380L (TGG→TTG)  cynX → cyanate transporter
RA 447,009 A→T Y233N (TAT→AAT)  cyoE ← heme O synthase
RA 547,658 C→A P327T (CCT→ACT)  fdrA → putative acyl‑CoA synthetase FdrA
RA 574,267 C→A G104G (GGC→GGA)  ybcQ → DLP12 prophage; putative antitermination protein
RA 598,114 G→T A212A (GCG→GCT)  cusB → copper/silver export system membrane fusion protein
RA 632,683 C→A V265V (GTG→GTT)  hcxA ← hydroxycarboxylate dehydrogenase A
RA 765,914 C→A intergenic (‑39/‑70) mngR ← / → mngA DNA‑binding transcriptional repressor MngR/2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 898,314 G→C W109S (TGG→TCG)  ybjO → putative inner membrane protein
RA 979,123 C→A P933Q (CCG→CAG)  mukB → chromosome partitioning protein MukB
RA 1,072,489 C→A Q125H (CAG→CAT)  rutB ← peroxyureidoacrylate/ureidoacrylate amidohydrolase
RA 1,120,278 G→A V103V (GTC→GTT)  solA ← N‑methyl‑L‑tryptophan oxidase
RA 1,265,001 C→A intergenic (+31/‑11) hemA → / → prfA glutamyl‑tRNA reductase/peptide chain release factor RF1
RA 1,304,457 C→A G208G (GGC→GGA)  oppC → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppC
RA 1,678,134 G→T intergenic (‑231/‑293) pntA ← / → ydgH pyridine nucleotide transhydrogenase subunit alpha/DUF1471 domain‑containing protein YdgH
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,750,451 G→T A188E (GCG→GAG)  ydhX ← putative 4Fe‑4S ferredoxin‑type protein
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,909,685 G→T R139S (CGT→AGT)  kdgR ← DNA‑binding transcriptional repressor KdgR
RA 1,954,643 T→C I789V (ATC→GTC)  torZ ← trimethylamine N‑oxide reductase 2
RA 1,955,924 C→A E362* (GAA→TAA)  torZ ← trimethylamine N‑oxide reductase 2
MC JC 1,978,503 Δ776 bp insB‑5–insA‑5 insB‑5, insA‑5
RA 2,057,292 C→A D145E (GAC→GAA)  yeeN → putative transcriptional regulator YeeN
RA 2,074,054 G→T W839L (TGG→TTG)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,213,853 G→C D264E (GAC→GAG)  btsS ← high‑affinity pyruvate receptor
RA 2,299,622 C→A G213G (GGG→GGT)  napG ← ferredoxin‑type protein NapG
RA 2,506,758 C→A L379L (CTG→CTT)  fryC ← putative PTS enzyme IIC component FryC
RA 2,617,540 C→A P483T (CCT→ACT)  bepA → beta‑barrel assembly‑enhancing protease
RA 2,656,493 G→T intergenic (‑135/+43) pepB ← / ← iscX aminopeptidase B/accessory iron‑sulfur cluster assembly protein IscX
RA 2,769,287 G→A R208H (CGC→CAC)  yfjQ → CP4‑57 prophage; protein YfjQ
RA 2,770,129 C→A L143M (CTG→ATG)  yfjR → CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,833,602 G→T R376L (CGA→CTA)  norV → anaerobic nitric oxide reductase flavorubredoxin
RA 2,835,672 C→A G585V (GGA→GTA)  hypF ← carbamoyltransferase HypF
RA 3,153,929 C→A L123M (CTG→ATG)  yghB → DedA family protein YghB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,395,064 C→A D399Y (GAT→TAT)  yhdP ← AsmA2 domain‑containing protein
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,565,132 C→A V484L (GTG→TTG)  glgP ← glycogen phosphorylase
RA 3,656,343 C→A intergenic (‑51/+65) hdeB ← / ← hdeA periplasmic acid stress chaperone/HdeA monomer, chaperone active form
RA 3,675,798 C→A P338Q (CCA→CAA)  yhjE → putative transporter YhjE
RA 3,705,758 G→T L11L (CTC→CTA)  dppB ← dipeptide ABC transporter membrane subunit DppB
RA 3,740,617 G→T V305F (GTC→TTC)  avtA → valine‑‑pyruvate aminotransferase
RA 3,754,000 G→T L34M (CTG→ATG)  yiaV ← putative membrane fusion protein
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,840,746 G→T R332S (CGC→AGC)  nepI ← purine ribonucleoside exporter
RA 4,142,527 C→A intergenic (‑306/‑3) ptsA ← / → frwC putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC
RA 4,261,710 C→A S14R (AGC→AGA)  dusA → tRNA‑dihydrouridine synthase A
RA 4,294,762 C→A V94V (GTC→GTA)  gltP → glutamate/aspartate : H(+) symporter GltP
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,332,509 C→A R255L (CGG→CTG)  basS ← sensory histidine kinase BasS
RA 4,347,593 C→G P384P (CCG→CCC)  dcuB ← anaerobic C4‑dicarboxylate transporter DcuB
RA 4,445,749 G→T A546A (GCG→GCT)  tamB → translocation and assembly module subunit TamB
RA 4,493,330 C→A A8S (GCA→TCA)  idnO ← 5‑keto‑D‑gluconate 5‑reductase
RA 4,565,819 A→T F11Y (TTC→TAC)  yjiM ← putative dehydratase subunit
RA 4,569,073 G→T pseudogene (76/921 nt) rpnD → truncated recombination‑promoting nuclease‑like protein RpnD
RA 4,571,333 G→A P81L (CCG→CTG)  yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,591,735 G→T G27C (GGT→TGT)  tsr → methyl‑accepting chemotaxis protein Tsr

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102983 3104082 1100 19 [18] [18] 20 mutY mutY
* * ÷ NC_000913 3423761–3424234 3424525–3424239 6–765 20 [17] [16] 21 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =19 (0.250)85 (1.240) 51/246 0.1 82.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 20 (0.290)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780519 (0.250)64 (0.930) 49/246 0.1 77.7% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 20 (0.290)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)107 (1.420) 61/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =142 (1.830)119 (1.550) 57/276 0.1 62.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)75 (0.970) 41/278 0.5 93.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA