breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
JC JC 1,879,829 Δ1 :: IS186 (+) +6 bp :: Δ1 coding (115‑120/360 nt) yeaR ← DUF1971 family protein, nitrate‑inducible
RA 2,019,460 G→A V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,108,006 A→T D108E (GAT→GAA glf ← UDP‑galactopyranose mutase, FAD/NAD(P)‑binding
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,222,566 C→A intergenic (+117/‑67) ebgR → / → ebgA transcriptional repressor/evolved beta‑D‑galactosidase, alpha subunit
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,890,075 IS5 (–) +4 bp coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,966,720 C→T R102C (CGC→TGC)  rho → transcription termination factor
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
JC JC 4,543,608 Δ1 :: IS186 (+) +6 bp :: Δ1 coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 583085 583876 792 56 [46] [50] 58 [tfaX]–[appY] [tfaX], [appY]
* * ÷ NC_000913 720880 721157 278 53 [49] [51] 53 [kdpE] [kdpE]
* * ÷ NC_000913 1978409 1979305 897 57 [47] [41] 53 insB1–insA insB1, insA
* * ÷ NC_000913 2469122 2469829 708 65 [45] [50] 57 [gtrB]–[gtrS] [gtrB], [gtrS]
* * ÷ NC_000913 3268771 3269509 739 52 [50] [49] 53 yhaC pentapetide repeats‑related protein
* * ÷ NC_000913 3423742–3424234 3424552–3424238 5–811 52 [51] [51] 52 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3455378 3455795 418 52 [48] [51] 54 [gspA]–[gspC] [gspA], [gspC]
* * ÷ NC_000913 3653659 3653783 125 52 [51] [45] 53 [yhiS] [yhiS]
* * ÷ NC_000913 3799033 3799369 337 69 [37] [39] 59 [waaU]–[waaZ] [waaU], [waaZ]
* * ÷ NC_000913 4476754 4477156 403 57 [44] [45] 55 yjgL SopA‑central‑domain‑like hexapeptide repeat protein
* * ÷ NC_000913 4572169 4572338 170 55 [44] [51] 52 yjiS/yjiT DUF1127 family protein/pseudogene

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =5 (0.040)110 (1.040) 50/268 0.8 93.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 10 (0.090)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078055 (0.040)80 (0.760) 48/268 0.9 91.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 10 (0.090)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 3670423 =141 (1.190)115 (0.980) 69/298 0.3 44.6% coding (832/1650 nt) treF cytoplasmic trehalase
?NC_000913 = 3873421 146 (1.250)coding (192/618 nt) dgoA 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase