breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 907,236 G→A G349G (GGC→GGT ybjT ← putative NAD‑dependent oxidoreductase
RA 1,132,064 G→T E71* (GAA→TAA)  flgD → flagellar hook assembly protein
RA 1,907,447 C→A intergenic (‑12/+1) cspC ← / ← yobF stress protein, member of the CspA‑family/DUF2527 family heat‑induced protein
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,151,223 (T)6→5 coding (39/462 nt) yegJ → uncharacterized protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,050,297 C→T W124* (TGG→TAG)  gcvT ← aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,606,809 C→A E298* (GAG→TAG)  rsmC ← 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2469976 2470503 528 51 [50] [50] 51 [gtrS] [gtrS]
* * ÷ NC_000913 3185150 3185456 307 52 [50] [50] 51 [ygiL] [ygiL]
* * ÷ NC_000913 3252610 3252917 308 54 [49] [49] 54 [yhaH]–[yhaI] [yhaH], [yhaI]
* * ÷ NC_000913 3423764–3424234 3424555–3424238 5–792 51 [50] [50] 51 [rrfD]–[rrlD] [rrfD], [rrlD]
* * ÷ NC_000913 3804607 3805192 586 52 [48] [49] 55 [waaS]–[waaP] [waaS], [waaP]

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =17 (0.150)110 (1.090) 71/274 0.1 86.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 19 (0.190)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780516 (0.140)82 (0.810) 52/274 0.6 83.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 19 (0.190)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)43 (0.390) 33/298 2.8 98.9% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 1 (0.010)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein