Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F9 I0 R1	103	124.7	2465218	97.5%	2403587	230.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	989,943	T→G	20.8%	S138S (TCA→TCC) ‡	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,943	0	T	G	20.8%	133.4 / 7.7	90	S138S (TCA→TCC) ‡	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (23/43); new base G (16/7); total (39/51)
Fisher's exact test for biased strand distribution p-value = 6.57e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCG  >  NC_000913/989707‑990178

gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                                                             <  1:666187/238‑1 (MQ=255)

gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTg                                                                                                                                                                                                                                             <  1:348975/238‑1 (MQ=255)

  tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGag                                                                                                                                                                                                                                           <  1:116440/238‑1 (MQ=255)

       tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatag                                                                                                                                                                                                                                     <  2:900339/239‑1 (MQ=255)

         ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcg                                                                                                                                                                                                                                   <  2:251466/239‑1 (MQ=255)

           cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTAAAGCCGAGAAAGCGGATAA‑ATTGC‑TTTCCA‑GCGGGTCGA‑GGGGTTGCGCAAAGTCGGCCTGCGGGGggcgagagcggt                                                                                                                                                                                                                                 >  2:890454/1‑237 (MQ=255)

            gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  1:444842/239‑1 (MQ=255)

            gTGAGGGAAAGCCGACGCGCCAGATCGTAGTGGCTGGTGCCCACAAACCGGGATTCCTGTTGCAGACGCTTAACGATGGCTTCTTGTACCTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  1:420003/239‑1 (MQ=255)

             tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                <  1:789900/238‑1 (MQ=255)

                     aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATT‑CTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCCGGAAGGAGAATAATTTTGT‑TTTCCA‑‑CGTGGCGG‑GGGGTTGTGCAAAGTCGGCCTGGGGGGAGCGTAAGCGGTGGACCC‑tttt                                                                                                                                                                                                                      >  2:1118632/1‑237 (MQ=255)

                          aCGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGCGAAGTCGAACAA‑TTTGC‑TTTCCA‑GCGGGTGGA‑GGGCGTGCGCAACGTCCGCCTGCGGGGGGGGCTAGCCGGGTGCCA‑TTTCCCttg                                                                                                                                                                                                                 >  1:1025507/1‑237 (MQ=255)

                           cgcgCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGAGGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGa                                                                                                                                                                                                                 <  2:91303/238‑1 (MQ=255)

                             cgcCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGGGAAGTCGGCTCA‑TTTTC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGGAAACGCGGGCTGCCGGGGGCGGCAGCGGTGTACCC‑TTTCTCTGATa                                                                                                                                                                                                               >  1:1126734/1‑238 (MQ=255)

                                  acgcGTAGTTGCGGGGCCCCACAAACCAGGATACCGTTGGCAGCCGTTTAACGATGGTTTTTTGTACTTCGCGAGAAAAACGGCGCC‑GGGGGCCAAAACCCATCAGATGGAAGCGCGACATACC‑AAGCCCGGCGGTTACCGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGGCGA‑GGGCTTGCGCAACTCCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGcc                                                                                                                                                                                                         <  1:1036234/236‑1 (MQ=255)

                                        aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAACCGGCGAC‑GGGTGCCAAAACCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCaa                                                                                                                                                                                                    <  2:952787/238‑1 (MQ=255)

                                        aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGGCGGCTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGGTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAAACGCCCGCaaa                                                                                                                                                                                                   >  2:1005922/1‑239 (MQ=255)

                                               ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGGTAGCGATGTACCC‑TTTCACTGATAACCGCCCGCAAAGGAACt                                                                                                                                                                                             >  2:477396/1‑238 (MQ=255)

                                               ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTAACA‑TTTCACTGATAAACCCCCGCCAAGGaaat                                                                                                                                                                                             >  1:1083384/1‑236 (MQ=255)

                                                                    cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCC‑GCGTGTCGG‑GGGCGTGCGCAACGGCGGCCTGCGGTGGGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACATCCCAGAGGGTGAATTCACGc                                                                                                                                                                        >  2:1147389/1‑238 (MQ=255)

                                                                      gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTAAAGCCGCGAAGTCGACTAA‑ATTGC‑TTTTCA‑GCGGGGCGG‑GGGCCTGCGCAAAGTCGGCCTTCGGTGTGCGGTAGCGGAGGACCA‑GTTTAATGATAAACCCCCGGCAAGGAACTTTCCCGGGGATGGATTTACGccc                                                                                                                                                                      >  1:563338/1‑237 (MQ=255)

                                                                        tgaaGACGGTTACGGAGGGTTTTTTGTACTGCGCGAAAAAACGGGCGAG‑GGGTGCAAAAATCCATCAGAGGGAAGCGCGACATACC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTCCCA‑GCGTGTGGA‑GGGCTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                                                                    <  1:696236/235‑1 (MQ=255)

                                                                         gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCGG‑GGGGTTGCGCAACGACCGCCTGCCGGGGGCGGTAGCGCTGGAACA‑TTTCACTCATAACCGCCCGCAAAGGCACTTCCCCGCGGGTGACCTctcccccccg                                                                                                                                                                   >  1:373855/1‑229 (MQ=255)

                                                                            gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCCGGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGGGTCGG‑GGGGTTGCGCCAAGGCGGCCCGCGGGGAGCGCAAGCGATGTAACC‑TTTCTCTGATAAACCCCAGCAAAGGGAATTCCCCGGGGATGACTTCACGCCCCGGGcc                                                                                                                                                                >  1:383357/1‑238 (MQ=255)

                                                                            gACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCCAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGCGTCGG‑GGGCTTGCGCCACGTCGGCCCGCGGGGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCACGGGcc                                                                                                                                                                >  2:590856/1‑238 (MQ=255)

                                                                                             tCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGCGAAATCGCCTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCCACCGCGGCCTGCGGGGAGCGGTAGCGGTGTAACA‑TTTCACTGGTAACCCCCCCCCAAAGAACTTTCCCGAGGGTGAATTTTAGCCCCGGGCCCCTTTAAAGAatttaca                                                                                                                                               >  1:378376/1‑233 (MQ=255)

                                                                                               tttgaCTGCGCGAAAAAAGCGGCTAC‑GGGTCCCAATATCCAACAGAGGGAAGCGCGACAAATA‑GAGACCGGCGGTTAACGCCGAGAGGTCGACTAA‑TTTGC‑TTCCCA‑GCGGGTCGA‑GGGTTTGCGCAATTTCGGCCTGCGGTGAGCGATGAAGTTGTACAA‑TTTCCCTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCt                                                                                                                                            <  1:800021/235‑1 (MQ=255)

                                                                                                     ttCGCGAGAAAAACGGCGAG‑GGGTGCCAAAATCCATCAGAGGGAAGTG‑GAAATACCAAAGACCCGGGGTTAGCGCAGGAAGGTCCA‑TTA‑TTTTT‑TTTCCACGGTTCTCGA‑GGGCTTGCGAAACTTCGGCTTGGGGGGGGGGATGGGAATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                      <  1:418862/239‑1 (MQ=255)

                                                                                                     tgcgcgAGAAAACCGGGGGC‑GGGGGAAAAAACCAAACGAATGGAAGCGGGACATATC‑AGGCCGGGCGGTTACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTGGA‑GGGTTTGCGCAACGTCGGCCGGGGGTGAGCGAGAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                      <  1:259729/237‑1 (MQ=255)

                                                                                                       ggcgAG‑AAAACGGCGGCGGGGGGCCAAAAGCCATCAGACGAAAGCGCGACATTTC‑AAGCCCGGCGGTAAACGCCGAGAAGCCGCTTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGCCTTGCGCAACGTCGGCGTGCGGTGAGCGATAGGGATGTACCT‑TTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTa                                                                                                                                    <  1:606028/238‑1 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGCGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGGTAGCGATGTACCA‑TTTCACTGATAACCCCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGGCGCCTAAAAGGAAATCCAACTTGCCAttt                                                                                                                                    >  2:1103764/1‑238 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCCAGAAGTCGACTAA‑TTTTC‑TTTTCA‑GCCGGTCGA‑GGGCTTGCGCAACCACGGCCTGCGGGGAGCGATAGCGGTGTACCA‑TTTTACTTATAAACCCCCGCAAAAGAAATTCCCACAGGAAGACTTCACCCCACCGGCCGACTAAAAGAATATCCAACCTGTCATTa                                                                                                                                    >  1:737493/1‑239 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGGGAAGTCGAATAA‑TTTTG‑TTTTCA‑GCGGGTCGG‑GGGGTTGGGCAACGGCGGGCTGCGGGGAGCGGTAGCGAAGGAACA‑TTTTACTGGTAAACGCCAGGAAAAGAACTTTCCAACAGATGAATTCCCGCCCACGGCCCCCTAAAAGAattaccacattgaccatt                                                                                                                                    >  1:493135/1‑223 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTGC‑TTTTCA‑GCGTGTCCA‑GGGCTTGGGAAACATCGGCCTGCGGTGAGAGCTAAGGCTGTTCCA‑TTTCACTTAATAACGCCACACAAGAAACATCCCCGCGGGTGACCTCAACCCCCGCGGCCCTTAAACGGAAATCCaacctcacctta                                                                                                                                    >  1:742550/1‑228 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAAAGCCCAGAAACCGAAGAA‑TTTTG‑TTTTCA‑ACGGGGCGG‑GGGCTTGCCGCACGTCGGCCCGCCGTGGGCGACAGGGATGGACCA‑ATTCACAGATCACAGCCCGCAAAGGAAATTCCCACAGGAGGAATTCACCCCCCCGGCCCGTTAAACGaaatactcagctggcatta                                                                                                                                    >  1:1043360/1‑222 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTAACGCCCCGCAATCCACCAA‑TTTTT‑GTTCCC‑GCGTGTCGG‑GGGCTTGCGCCACGTCGGCCTGCGGTGAGCGGTAACGCTGGACCC‑TTTCTCTGATAAACGCCAGCAAAAGAACATCCCCGAGGCGGACTTACCCCCACGGGCCGCCTTAAcacaaatccccccctccctca                                                                                                                                    >  1:903308/1‑219 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGGTAACACCGAAAAGTAGACCAA‑TTTGC‑TTTTCA‑ACGTGGTGA‑AGGCTTTCGCAAAGTCGGGCTGCGGTTCGGGGTAGCGCGGAACCA‑GCTCATCGATTACCGCCACCAAACGAAGTTCCCCGAGGATGACGTCACTCCACGGCACCCTTAAAAGAATATCCAGCTTGGCAtgt                                                                                                                                    >  1:699412/1‑237 (MQ=255)

                                                                                                       cgcgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGCTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCCTGTCGA‑GGGGTTTCGCAAAGTCGGCCTGCGGGGGGCGCTAGCGATGTAACA‑TTTCACTGATAACCCCCAGCAAAGGAACTTCCCAGAGGATGACTTTACCCCCCGGGCCGCCTAAAAGAATATCCAGCTTGGCATTa                                                                                                                                    >  1:125872/1‑239 (MQ=255)

                                                                                                         cgAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCCGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGTTAACCGCCCGCAAAGGAACTTCCCAGAGGGTGACTTCACCCCACGGGCCGCCTAAAAGAAAAATCAGCTTTCCATTATc                                                                                                                                  >  2:105888/1‑239 (MQ=255)

                                                                                                             aaaaaCGGCGGG‑GGGTCGCAAACCCCACAAGATGGAACGGCGACATACA‑AAAGCGGGCGGTTAACGCAGAGAAGTCG‑CTTA‑TTTGCTTTTCCA‑GCGTGTGGA‑GGATTTGGCCAAGGTCGGCCTGGGGTGGGCGAAAGGGATGTCCCA‑TTTCATGGAGAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                                                              <  1:136731/239‑1 (MQ=255)

                                                                                                                                           agagGGAAGGGGGACATAAC‑AAGACGGGGGGTAACCGCGGAGAAGCCGACTAT‑TTTGC‑TTCCAG‑GGGGGGCGG‑GGGCTGGCGAAACGTCGGCCGGCGGGGGGCGATGGCGATGTCCCA‑TTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAg                                                                                                   <  1:590856/237‑1 (MQ=255)

                                                                                                                                               ggAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGGCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCGTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAAACACCCGCAAAAGAACTTCCCAAAGGGAGACTTCACGCCCCCGGGCGCCTAAAAGAAAATCCACCTCGCCATTATCGTTTGTCACGGTGACTTGTTTCGGGTTTAAGCGGaa                                                                                              >  1:79422/1‑238 (MQ=255)

                                                                                                                                                 aaGCGCGACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAAAGTCGGCCCGCGGGGGGCGAAAGCGATGTACCA‑TTTCACTGATAACCGCCAACAAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCTTCAACGAAAATCCCACTTTCCATTTTCGTTTGACACGGTGACTTGGTCCGGGGTAAAGCGGaaacc                                                                                           >  1:48225/1‑236 (MQ=255)

                                                                                                                                                      gccacatacaaagccggggtgTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTcgc                                                                                         <  1:620542/217‑1 (MQ=255)

                                                                                                                                                      cgACATATC‑AAGACCGGCGGTTAAAGCCGAGAAGTCGACTAA‑TTTTC‑TTTTCC‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCA‑TTTTACTGATAACCGCCCGCAAAAGAAATTCCCAGAGGGTGACTTCCCGCCACGGGCCCCTTAAAAGAATATCCAGCTTTCCATTATCGTTGGACACGGTGACTTGTTCCGGGGTTAAGCGGAACTCGCGTa                                                                                      >  2:822188/1‑239 (MQ=255)

                                                                                                                                                       gacaagtC‑AAGACCGGGGGATAACGCCGAGAAGTCGACGAT‑TTTGC‑TTTCCG‑GGGGGTCGA‑GGGCTTGCGCAACGCCGGCCTGCGGTGGGTGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  1:671591/233‑1 (MQ=255)

                                                                                                                                                       gACATTAC‑AAGCCGGGGGGTTATCGCCGAGAGGTCGGCTAA‑TTGTC‑TTGCCG‑TCTGTTCGG‑GGGCTGGCAAAACGCCGCCGGGGGGGGGGCGATAGCGTGGTACCA‑TTCCTCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  1:1118632/239‑1 (MQ=255)

                                                                                                                                                       gACATATC‑AGGCCCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTGGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCT‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  2:48225/239‑1 (MQ=255)

                                                                                                                                                        atatatA‑AAGACCGGCGCTCACGGGCGAGAAGTCGCCTAA‑TTTGC‑TCTCCA‑GCGTTTCGA‑GGGCTCGCGCAAGGTGGGCCGGCGGTGAGCCAAGGCGATGTACTA‑TTTCACTGAGCGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTGCGCGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  1:608915/236‑1 (MQ=255)

                                                                                                                                                         acagtaaaggcccGGGGTTTCACGCCGAGAAGCCACTTAT‑TTTTC‑TTCCCT‑GCGTGGCGT‑GGGTTTGCGAAACGGCGGCCTGGGGTGAGTGAATGCGTGTTACCA‑TTTAACTGAGACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  1:128173/227‑1 (MQ=255)

                                                                                                                                                         aaatacgaaGACGGGCGGTTAGCGCCGAGAAGTCGGCTAT‑TTTGC‑TTCCCG‑GCGTGGCGA‑GGTCTTGCGAAACGCCGGCCTGGGGTGAGCGATAGCGAGTTACCA‑TTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                     <  1:1147389/231‑1 (MQ=255)

                                                                                                                                                          aatagcagacccGGAGTTTAACGCCGAAAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTGGCGAAGCGCCGGCCGGGGGTGAGGGATAGGGATGTACCA‑TTCCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                    <  1:105888/228‑1 (MQ=255)

                                                                                                                                                             tC‑AAGACCGGCGTTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTCCCA‑TTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGtt                                                                               <  2:506583/239‑1 (MQ=255)

                                                                                                                                                              c‑AAGGCCGGCGGTAAACGCCGAGAAGCCGTCTAA‑TTTGT‑TTCCCA‑GGGTGTCGA‑GGGCTTGCGCAACCTCGGCGGGGGGGGGGCGAAAGCGATGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                                                              <  1:101964/239‑1 (MQ=255)

                                                                                                                                                                     cggcggTTAACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GAGTGGCGA‑GGGCTTGCGCAACGTCGGGCCGCGGGGAGCGCTAGCGATGGACCA‑TTTCACTGATAACCGCCCGCAAAGGAAATTCCCAGAGGATGACTTCACGCCACCGGCCGCTTAAACGAATATCCAGCTTGCCCTTATCGTTGGCCACGGGGACCTGTTCCGGGGTAAAACGGGACTCGCGGAAACAGTTAAGATaa                                                                        >  1:770658/1‑238 (MQ=255)

                                                                                                                                                                      ggcggTTCACGCCGAGAAGTCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCTCTGATAAACCCCCGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGGCGCTTAAACGAATATCCAGCTTGCCATTATTGTTGGACCCGGTGACTTTTTCCGCGTTAAAGCGGCCCACGCTGAACCAGCTAAAATat                                                                        >  1:865326/1‑237 (MQ=255)

                                                                                                                                                                       ggggTTACCGCCGAGAAGCCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                       <  2:1083384/236‑1 (MQ=255)

                                                                                                                                                                       gcggTTAACCCCGAGAAATCGACTAA‑TTTTC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGGATCC‑TTTCACTGATAACCGCCAGCCAAGGAACTTCCCAGAGGGTGACTTCACGCCCCCGGCCGCCTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc                                                                      >  1:162345/1‑239 (MQ=255)

                                                                                                                                                                       gcggTGACCGGCGAGAAGTGGATAAT‑TTTGC‑TTTCCG‑GGGTGTCGA‑GGGTTGGCGAACCGCCGGCCTGGGGAGGGCGATAGCGATGACCCA‑TTTCACGGATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                       <  1:508199/238‑1 (MQ=255)

                                                                                                                                                                            tagcgacGAGAAGTCTACTAA‑TTTGC‑TTTCCA‑GCGGTTCGA‑GGTCTTACGCACCGTCGGCCGGCGGTGAGGGATAGCGATGTACCA‑TTCCACTATTAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  2:956524/233‑1 (MQ=255)

                                                                                                                                                                            taaggcgaagagGTCGACTAA‑GTTGC‑TTTCCT‑GCGTGTCGG‑GGGCTGGCGCAGCGGCTGCCGGGGGTGAGCAATAGGGATG‑ACCATTTTCACTAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  1:373052/231‑1 (MQ=255)

                                                                                                                                                                            tAACGCCGAGAAGTCGTCTAT‑TTTGC‑TTTCCG‑GCGTGTCGG‑GGGTTTGCGAAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCT‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  2:383357/239‑1 (MQ=255)

                                                                                                                                                                            tAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  2:770658/239‑1 (MQ=255)

                                                                                                                                                                            tAACGCCAAGAAGCCGACTAA‑TTTGC‑TTTCCG‑GCGTGTCGA‑GGCCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                 <  2:860154/239‑1 (MQ=255)

                                                                                                                                                                                 ccGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag                                                            >  1:261482/1‑239 (MQ=255)

                                                                                                                                                                                         ccGACTAT‑TTTGC‑TTCCAA‑GCGTGTGGA‑GGGCTGCCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAata                                                                                                                                                   <  1:1051242/143‑1 (MQ=255)

                                                                                                                                                                                          cGACTAA‑TTTGC‑TTTCCA‑‑CGGGTCGACGTGCGGGGCAAACGTCGGCCGGCGGTGCGGCATAGCGTTGGCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggca                                                     <  1:553477/237‑1 (MQ=255)

                                                                                                                                                                                                a‑TTTGC‑TTCCCG‑GGGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGGGATAGCGATGTACCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                             <  2:756915/239‑1 (MQ=255)

                                                                                                                                                                                                a‑TTTGC‑TTCCCA‑GCGTGTCGG‑GGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTCCCA‑TTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                             <  2:31666/239‑1 (MQ=255)

                                                                                                                                                                                                    tGC‑TTTCCG‑GCGTGTGGG‑GGGCTTGCGCAACGTCGGCCTGGGGTGGCAGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                          <  2:368407/239‑1 (MQ=255)

                                                                                                                                                                                                    tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGc                                                                                                                                         >  2:1051242/1‑144 (MQ=255)

                                                                                                                                                                                                    tGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGACCTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAg                                           >  1:458893/1‑238 (MQ=255)

                                                                                                                                                                                                      c‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                        >  1:168276/1‑95 (MQ=255)

                                                                                                                                                                                                      c‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCGGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                        <  2:168276/95‑1 (MQ=255)

                                                                                                                                                                                                        ttcccA‑GCGTGTGGG‑GGGCTTGCGCAACGCCGGCCGGCGGTGAGCGATAGCGATGTACCA‑TTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                       <  2:1025507/236‑1 (MQ=255)

                                                                                                                                                                                                         tttcaggCGTGTCGA‑AGGCTGGCGCACGGTCGGCCTGCGGGGAGCGATGGGGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                       <  2:499859/233‑1 (MQ=255)

                                                                                                                                                                                                          tCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                     >  2:635268/1‑239 (MQ=255)

                                                                                                                                                                                                          tCCA‑GCGTGTCGA‑GGGCTTGCCCAACGTGGGCTTGCGGTGGGCGATAGCGATGTCCCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTg                                     <  2:125872/239‑1 (MQ=255)

                                                                                                                                                                                                                               gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAAc                                                                                    >  2:736279/1‑173 (MQ=255)

                                                                                                                                                                                                                               gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                   >  2:232185/1‑238 (MQ=255)

                                                                                                                                                                                                                               gcgaAACGTCGGCCGGGGGGGAGCGAGAGCGATGTCCCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAgg                  <  1:822188/239‑1 (MQ=255)

                                                                                                                                                                                                                                cgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgc                 >  2:1188491/1‑239 (MQ=255)

                                                                                                                                                                                                                                 gcAACGTGGGCCTGCGGTGAGCAATAGCAATGTCCCC‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcg                <  2:733799/239‑1 (MQ=255)

                                                                                                                                                                                                                                  caatggccgccgggggTGAGCAATGGCGTTGTCCCA‑TTCCACTCTAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc               <  1:747768/225‑1 (MQ=255)

                                                                                                                                                                                                                                   atgtccGGCCTGCGGTGAGCAAAAGCGTGTTCCCA‑TTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc               <  2:1225322/233‑1 (MQ=255)

                                                                                                                                                                                                                                   aaCGTCGCCCTGCGGTGAGCGATAGCGATGTCCCA‑TTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcgc               <  2:584196/238‑1 (MQ=255)

                                                                                                                                                                                                                                            cTGCGGTGGGGGATAGCGATGTCCCA‑TTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa     <  1:1221535/239‑1 (MQ=255)

                                                                                                                                                                                                                                            cTGCGGTGAGCGATGGCGATGTACCA‑TTTCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa     <  2:373855/239‑1 (MQ=255)

                                                                                                                                                                                                                                               cGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc   >  1:25405/1‑238 (MQ=255)

                                                                                                                                                                                                                                               cGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc   >  1:934389/1‑238 (MQ=255)

                                                                                                                                                                                                                                                 gTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCg  >  2:825185/1‑237 (MQ=255)

GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGAC‑GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATC‑AAGACCGGCGGTTAACGCCGAGAAGTCGACTAA‑TTTGC‑TTTCCA‑GCGTGTCGA‑GGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCA‑TTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCG  >  NC_000913/989707‑990178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A