Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A18 F130 I1 R1
|
38 |
76.4 |
2725422 |
95.3% |
2597327 |
137.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_pae_tpiA |
4,171,489 |
G→A |
intergenic (+29/‑64) |
rrlB → / → rrfB |
23S ribosomal RNA of rrnB operon/5S ribosomal RNA of rrnB operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_pae_tpiA | 4,171,489 | 0 | G | A | 95.7%
| 86.9
/ ‑2.8
| 46 | intergenic (+29/‑64) | rrlB/rrfB | 23S ribosomal RNA of rrnB operon/5S ribosomal RNA of rrnB operon |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (20/24); total (21/25) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
GATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTA > NC_000913_3_pae_tpiA/4171415‑4171630
|
gATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCa < 4:467891/150‑1 (MQ=11)
aTGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAg > 2:763187/1‑150 (MQ=14)
aTGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAg > 2:262678/1‑150 (MQ=14)
aTGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAg < 1:741257/150‑1 (MQ=14)
tGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAgcgc < 2:158876/150‑1 (MQ=17)
gAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGcgcg < 3:98205/150‑1 (MQ=32)
gCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCggt < 1:630212/150‑1 (MQ=17)
cTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCggtg > 1:582596/1‑150 (MQ=17)
ccGGTACTGATGAAGCGGGAGGCTTAACCTTACAACGCCGATGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATGTGCCTGGCGGCAGTAGCGCGGTGGTcc < 2:63794/150‑1 (MQ=11)
tAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGa < 4:202667/150‑1 (MQ=17)
tAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGa > 1:428213/1‑150 (MQ=17)
tAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGa > 1:303612/1‑150 (MQ=17)
tAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGCAGCGCGGTGGTCCCACCTGa > 1:428412/1‑150 (MQ=17)
aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGAcc < 3:210784/150‑1 (MQ=17)
tGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGAccc < 3:387416/150‑1 (MQ=17)
gAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTc < 4:354540/138‑1 (MQ=18)
gAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTc > 3:354540/1‑138 (MQ=18)
cGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGc < 4:271989/150‑1 (MQ=17)
gTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGcc > 1:6173/1‑150 (MQ=17)
tGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGTCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCg < 3:120245/150‑1 (MQ=17)
tGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAACCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCg > 1:490185/1‑150 (MQ=17)
cTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTc > 1:154042/1‑150 (MQ=17)
cTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTc > 1:154015/1‑150 (MQ=17)
cTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAGGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACTCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCCAACTc > 4:52578/1‑150 (MQ=12)
ttAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCa > 1:535045/1‑150 (MQ=17)
ttAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGCCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCa < 2:572413/150‑1 (MQ=17)
aaCCTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAAGTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGa > 2:518109/1‑150 (MQ=17)
ccTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAg < 2:31385/150‑1 (MQ=17)
cTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGt < 4:214696/150‑1 (MQ=17)
cTTACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGACTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGt > 2:565575/1‑150 (MQ=12)
ttACAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTg < 1:763187/150‑1 (MQ=17)
aCAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGaa > 2:306316/1‑150 (MQ=17)
aCAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGaa > 3:16455/1‑150 (MQ=17)
cAACGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGaaa < 1:726974/150‑1 (MQ=17)
aaCGCCGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAAc > 1:116723/1‑150 (MQ=17)
ccGAAGCTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCg < 1:341417/150‑1 (MQ=17)
cTGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGTCCCCATGCCGAACTCAGAAGTGAAACGCCGTAgcgc < 1:777321/150‑1 (MQ=17)
tGTTTTGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGcc > 2:670089/1‑150 (MQ=17)
ttttGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGa > 1:822477/1‑150 (MQ=17)
tttGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGAt < 1:718697/150‑1 (MQ=17)
ttGGCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCCGGCGGCAGTAGCGCGGTGGTCCCACCTGCCCCCATGCCTGACTCAGAAGTGAAACGCCGTAGCGCCGATg < 2:448190/150‑1 (MQ=11)
ttGGCGGATGAAAGAAGATTTTCAGCATGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATg < 3:160080/150‑1 (MQ=17)
ggCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGt > 2:461653/1‑150 (MQ=17)
gCGGATGAAAGAAGATTTTGCGCCGGATACAGATTAAATCAGAACGCAGGAGCGGTCTGATAAAACAGAATTCGCCTGGCGGCAGTAGCGCGGTGGTCCCATCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTa < 2:428412/150‑1 (MQ=11)
gCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTa < 2:428213/150‑1 (MQ=17)
gCGGATGAAAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTa < 1:262678/150‑1 (MQ=17)
|
GATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTA > NC_000913_3_pae_tpiA/4171415‑4171630
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
