Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F87 I1 R2 17 76.1 3237006 92.4% 2990993 123.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,186,457 A→G K398E (AAG→GAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,186,4570AG76.2% 111.3 / 35.1 63K398E (AAG→GAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (8/7);  new base G (22/26);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAG  >  NC_000913_3_pae_tpiA/4186312‑4186601
                                                                                                                                                 |                                                                                                                                                
ctCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCggagaa                                                                                                                                              <  1:263341/150‑1 (MQ=255)
 tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGaaa                                                                                                                                             >  1:86238/1‑150 (MQ=255)
 tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGaaa                                                                                                                                             >  3:85044/1‑150 (MQ=255)
           cTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAgc                                                                                                                                   <  1:279135/150‑1 (MQ=255)
           cTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAgc                                                                                                                                   >  2:764761/1‑150 (MQ=255)
                        aGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGt                                                                                                                      <  3:342981/150‑1 (MQ=255)
                              aTACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg                                                                                                                <  1:764761/150‑1 (MQ=255)
                                aCCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                              <  3:163155/150‑1 (MQ=255)
                                   tGCGTCTGCATCAGTGCGGTCTGCCGAAGAAACTGGCACTGGTGCTATTCAATCACTACTACTATGGGCAGCTGCATCTGCGTGGGCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGata                                                                                                           <  1:850366/150‑1 (MQ=255)
                                              cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                >  2:545595/1‑150 (MQ=255)
                                              cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                <  3:517256/150‑1 (MQ=255)
                                               aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAg                                                                                               <  1:545595/150‑1 (MQ=255)
                                                     gTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATcc                                                                                         >  2:526253/1‑150 (MQ=255)
                                                      tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg                                                                                                                <  1:597103/126‑1 (MQ=255)
                                                      tCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg                                                                                                                >  2:597103/1‑126 (MQ=255)
                                                         gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATgg                                                                                                                                         >  3:179272/1‑98 (MQ=255)
                                                         gCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATgg                                                                                                                                         <  4:179272/98‑1 (MQ=255)
                                                                aaaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCg                                                                              <  2:71142/150‑1 (MQ=255)
                                                                 aaaTGGGACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCCTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                             <  3:592738/150‑1 (MQ=255)
                                                                     ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGt                                                                                                                      >  1:695126/1‑105 (MQ=255)
                                                                     ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGt                                                                                                                      <  2:695126/105‑1 (MQ=255)
                                                                      gCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACGATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActg                                                                        <  1:526253/150‑1 (MQ=255)
                                                                          tGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                               <  2:857289/139‑1 (MQ=255)
                                                                          tGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                               >  1:857289/1‑139 (MQ=255)
                                                                           ggAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGaa                                                                   <  3:110698/150‑1 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                               >  1:877687/1‑99 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                               <  2:877687/99‑1 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                            >  1:313101/1‑150 (MQ=255)
                                                                                     aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                              >  2:698941/1‑97 (MQ=255)
                                                                                     aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                              <  1:698941/97‑1 (MQ=255)
                                                                                     aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                               >  2:876597/1‑128 (MQ=255)
                                                                                     aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAccc                                                                               <  1:876597/128‑1 (MQ=255)
                                                                                      aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                       >  1:717506/1‑71 (MQ=255)
                                                                                      aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                       <  2:717506/71‑1 (MQ=255)
                                                                                       aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                                       <  2:769255/150‑1 (MQ=255)
                                                                                           ttCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTg                                                   >  4:275060/1‑150 (MQ=255)
                                                                                               tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATcc                                                                                                        <  2:457122/93‑1 (MQ=255)
                                                                                               tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATcc                                                                                                        >  1:457122/1‑93 (MQ=255)
                                                                                                cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                >  2:107397/1‑100 (MQ=255)
                                                                                                cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                <  1:107397/100‑1 (MQ=255)
                                                                                                cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCg                                              >  2:241728/1‑150 (MQ=255)
                                                                                                  aCGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGTCTCTGCACCGTc                                            >  4:368122/1‑150 (MQ=255)
                                                                                                   cGGCAAGCTCGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                           >  1:683459/1‑150 (MQ=255)
                                                                                                    ggCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTg                                          <  1:241728/150‑1 (MQ=255)
                                                                                                          cTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATc                                    >  1:771458/1‑150 (MQ=255)
                                                                                                             gAACTGCGTGGTCTTGCTACCACCATTAATGCTGCGGAGAAAATGGTTGAGTGTGAATCAGCTGTCGTTTGGGATATCCTGGTCGCAGTTTTCAGCGTACACCCGGTACTGCTGAACCGTGCATCGACTCTGCAACGTCTGTGTATcctg                                 <  2:920436/150‑3 (MQ=255)
                                                                                                                cTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCa                              >  2:115325/1‑150 (MQ=255)
                                                                                                                   cGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAttt                           <  3:275060/150‑1 (MQ=255)
                                                                                                                          ttGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCgg                    >  2:553828/1‑150 (MQ=255)
                                                                                                                          ttGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCgg                    >  3:243113/1‑150 (MQ=255)
                                                                                                                             cTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc                 <  2:313101/150‑1 (MQ=255)
                                                                                                                                ccaccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGa              >  2:158130/1‑150 (MQ=255)
                                                                                                                                  accaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                           >  2:655915/1‑119 (MQ=255)
                                                                                                                                  accaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                           <  1:655915/119‑1 (MQ=255)
                                                                                                                                      ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg        <  4:85044/150‑1 (MQ=255)
                                                                                                                                          aGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                >  3:69114/1‑106 (MQ=255)
                                                                                                                                          aGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                <  4:69114/106‑1 (MQ=255)
                                                                                                                                          aGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaa    <  4:243113/150‑1 (MQ=255)
                                                                                                                                            gtGCGGAGAAAATGGTTGAGCGGGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCAGCGAACACCCGGGACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAg  <  2:86238/149‑1 (MQ=255)
                                                                                                                                             tGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                                                <  4:294440/71‑1 (MQ=255)
                                                                                                                                             tGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                                                >  3:294440/1‑71 (MQ=255)
                                                                                                                                              gCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                      >  1:421781/1‑80 (MQ=255)
                                                                                                                                              gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGt                                             <  2:767021/105‑1 (MQ=255)
                                                                                                                                              gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGt                                             >  1:767021/1‑105 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAG  >  NC_000913_3_pae_tpiA/4186312‑4186601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A