Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F19 I1 R1 18 124.3 4698080 94.5% 4439685 130.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,187,130 A→G D622G (GAC→GGC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,187,1300AG97.4% 389.9 / ‑4.2 114D622G (GAC→GGC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base G (45/65);  minor base T (3/0);  total (48/66)
Fisher's exact test for biased strand distribution p-value = 7.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTG  >  NC_000913_3_pae_tpiA/4186984‑4187276
                                                                                                                                                  |                                                                                                                                                  
tGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCa                                                                                                                                                 >  2:696410/1‑150 (MQ=255)
 gTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTCTTTTTGCGGGCCAg                                                                                                                                                >  1:785678/1‑150 (MQ=255)
  ttGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGa                                                                                                                                               <  3:98987/150‑1 (MQ=255)
     gCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCa                                                                                                                                            <  2:1325320/150‑1 (MQ=255)
      ccGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCAt                                                                                                                                           >  1:270722/1‑150 (MQ=255)
       cGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATg                                                                                                                                          <  2:21452/150‑1 (MQ=255)
         tGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa                                                                                                                                        >  2:855279/1‑150 (MQ=255)
              ttCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCg                                                                                                                                   >  2:701696/1‑150 (MQ=255)
                 tgtgGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCt                                                                                                                                >  4:164213/1‑150 (MQ=255)
                   tgGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTc                                                                                                                              <  1:701696/150‑1 (MQ=255)
                     gatgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGc                                                                                                                            <  3:462641/150‑1 (MQ=255)
                      atgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGcc                                                                                                                           <  3:754610/150‑1 (MQ=255)
                      atgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGcc                                                                                                                           <  2:270722/150‑1 (MQ=255)
                       tgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCt                                                                                                                          <  2:721407/150‑1 (MQ=255)
                          ttGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg                                                                                                                       >  2:179326/1‑150 (MQ=255)
                          ttGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCACGGCCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg                                                                                                                       >  1:426946/1‑150 (MQ=255)
                            gTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCa                                                                                                                     <  1:1239780/150‑1 (MQ=255)
                            gTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCa                                                                                                                     <  1:1239770/150‑1 (MQ=255)
                              aCCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAgc                                                                                                                   >  4:239291/1‑150 (MQ=255)
                              aCCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAgc                                                                                                                   >  4:239352/1‑150 (MQ=255)
                              aCCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAgc                                                                                                                   <  1:696410/150‑1 (MQ=255)
                                 gAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGcgcg                                                                                                                >  1:1069777/1‑150 (MQ=255)
                                       tCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTgg                                                                                                          <  1:211709/150‑1 (MQ=255)
                                       tCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTgg                                                                                                          >  2:1203406/1‑150 (MQ=255)
                                        cTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt                                                                                                         >  4:455179/1‑150 (MQ=255)
                                        cTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt                                                                                                         <  1:1011242/150‑1 (MQ=255)
                                          gCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGTCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGc                                                                                                       <  2:992323/150‑1 (MQ=255)
                                          gCCTCACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa                                                                                                                                        >  3:654126/1‑117 (MQ=255)
                                          gCCTCACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa                                                                                                                                        >  3:654010/1‑117 (MQ=255)
                                          gCCTCACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTa                                                                                                                                        <  4:654010/117‑1 (MQ=255)
                                                 tCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt                                                                                                >  2:70883/1‑150 (MQ=255)
                                                 tCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTACAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt                                                                                                >  4:851328/1‑150 (MQ=255)
                                                  ccATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGGTCTGGTGCATCTGTTg                                                                                               <  2:737062/150‑1 (MQ=255)
                                                   cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTg                                                                                                           >  1:1355865/1‑137 (MQ=255)
                                                   cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTg                                                                                                           <  2:1355865/137‑1 (MQ=255)
                                                    aTCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGGCCGTTATTTTTGCGGGCCAGAACATGCACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGt                                                                                             >  1:1149718/1‑150 (MQ=255)
                                                    aTCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGt                                                                                             >  1:78138/1‑150 (MQ=255)
                                                      cGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGc                                                                                                                            >  1:150114/1‑117 (MQ=255)
                                                         cAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGa                                                                                        <  4:502221/150‑1 (MQ=255)
                                                         cAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCCGGTGCATCTGTTGGTATCGa                                                                                        <  1:663188/150‑1 (MQ=255)
                                                          aaCCAGGCGCTTGGTAAAAAAGCAAACTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGAt                                                                                       <  4:590651/150‑1 (MQ=255)
                                                            ccAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGa                                                                                     <  1:70883/150‑1 (MQ=255)
                                                             cAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGAc                                                                                    >  4:698360/1‑150 (MQ=255)
                                                                gcgcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATg                                                                                 <  3:239352/150‑1 (MQ=255)
                                                                gcgcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATg                                                                                 <  3:239291/150‑1 (MQ=255)
                                                                  gcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATgg                                                                                <  1:466440/149‑1 (MQ=255)
                                                                  gcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATgg                                                                                >  2:466440/1‑149 (MQ=255)
                                                                         aaaaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCg                                                                        <  1:921660/150‑1 (MQ=255)
                                                                           aaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGga                                                                      <  2:744215/150‑1 (MQ=255)
                                                                                cAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaaga                                                                 <  4:264190/150‑1 (MQ=255)
                                                                                cAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaaga                                                                 >  1:1340222/1‑150 (MQ=255)
                                                                                   tctcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAac                                                              >  4:603429/1‑150 (MQ=255)
                                                                                    ctcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAaca                                                             >  3:49459/1‑150 (MQ=255)
                                                                                     tcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac                                                            >  4:485050/1‑150 (MQ=255)
                                                                                     tcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAacac                                                            <  1:1203406/150‑1 (MQ=255)
                                                                                      ccAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACg                                                           <  2:1072816/150‑1 (MQ=255)
                                                                                       cAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTgg                                                                                                          >  1:632786/1‑102 (MQ=255)
                                                                                       cAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGa                                                          <  2:1441314/150‑1 (MQ=255)
                                                                                        aaaaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGaa                                                         <  1:207123/150‑1 (MQ=255)
                                                                                         aaaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGaaa                                                        >  2:603443/1‑150 (MQ=255)
                                                                                         aaaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGaaa                                                        <  3:29378/150‑1 (MQ=255)
                                                                                          aacgCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg                                                                                                                       >  1:530547/4‑86 (MQ=255)
                                                                                          aacgCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATg                                                                                                                       <  2:530547/83‑1 (MQ=255)
                                                                                           aTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatc                                                      <  4:335524/150‑1 (MQ=255)
                                                                                               tGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtct                                                  >  3:737084/1‑150 (MQ=255)
                                                                                               tGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtct                                                  <  4:737084/150‑1 (MQ=255)
                                                                                                gAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt                                                                                                >  3:664065/1‑103 (MQ=255)
                                                                                                gAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt                                                                                                <  4:664065/103‑1 (MQ=255)
                                                                                                 aaCACCTGCTACCGCATTCTCGGTCTGAAAGCGACCGTTATTTTTGCGTGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTcc                                                <  1:331300/150‑1 (MQ=255)
                                                                                                 aaCACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtcttc                                                >  4:827476/1‑148 (MQ=255)
                                                                                                  acacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCg                                               <  3:256221/150‑1 (MQ=255)
                                                                                                   cacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGa                                              <  1:603443/150‑1 (MQ=255)
                                                                                                   cacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGa                                              <  3:782903/150‑1 (MQ=255)
                                                                                                      cTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgc                                           <  2:785678/150‑1 (MQ=255)
                                                                                                         cTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTTTTTTTTTTTTCATGTACACCGGCTTCACCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGCTCACCTGGTCATCCCGGCGAAGAAACACGTAATCATCTCCGAGgcata                                        >  4:797491/1‑148 (MQ=255)
                                                                                                         cTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcaga                                        >  2:1443843/1‑150 (MQ=255)
                                                                                                           aCCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaag                                      <  2:1069777/150‑1 (MQ=255)
                                                                                                            ccGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTGTCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagc                                     >  3:288073/1‑150 (MQ=255)
                                                                                                             cGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCCCCGAGgcagaagca                                    >  1:1429826/1‑150 (MQ=255)
                                                                                                               cATTCTCGGTCTGAAACCGACCGTTATTTTTTCGTGCCTTATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagcaga                                  >  2:1023779/1‑150 (MQ=255)
                                                                                                                aTTCTCGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagcagaa                                 <  3:501240/150‑1 (MQ=255)
                                                                                                                  tctcGGTCTGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGTTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGt                               >  1:396765/1‑150 (MQ=255)
                                                                                                                     cGGTCTGAAGCCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCAACCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGc                            <  4:861559/150‑1 (MQ=255)
                                                                                                                       gTCTGAAACCGACCGTTATTTTTGCGGGCCAGATc                                                                                                                                             <  2:749704/35‑1 (MQ=255)
                                                                                                                       gTCTGAAACCGACCGTTATTTTTGCGGGCCAGATc                                                                                                                                             >  1:749704/1‑35 (MQ=255)
                                                                                                                          tGAAACCGACCGTTATTTTTTTTTTTCTTATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCAATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa                       >  4:408349/1‑150 (MQ=255)
                                                                                                                          tGAAACCGACCGTTATTTTTTTTTTCCATATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa                       >  2:748248/1‑150 (MQ=255)
                                                                                                                          tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCt                           <  1:1005311/146‑1 (MQ=255)
                                                                                                                          tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCt                           >  2:1005311/1‑146 (MQ=255)
                                                                                                                          tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa                       <  3:152196/150‑1 (MQ=255)
                                                                                                                          tGAAACCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGaaa                       >  2:1449650/1‑150 (MQ=255)
                                                                                                                             aaCCGACCGTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGAAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTc                    >  2:209377/1‑150 (MQ=255)
                                                                                                                              accgaccgTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCa                   <  3:698360/150‑1 (MQ=255)
                                                                                                                               ccgaccgTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAg                  <  1:179326/150‑1 (MQ=255)
                                                                                                                               ccgaccgTTATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCACCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAg                  <  4:49459/150‑1 (MQ=255)
                                                                                                                                      ttATTTTTGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGt           <  1:855279/150‑1 (MQ=255)
                                                                                                                                            ttGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgt     <  3:455179/150‑1 (MQ=255)
                                                                                                                                            ttGCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgt     <  3:454744/150‑1 (MQ=255)
                                                                                                                                              gCGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCt                           <  2:1319041/126‑1 (MQ=255)
                                                                                                                                               cGGGCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAgtctg  <  1:1290895/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGTTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAGTCTG  >  NC_000913_3_pae_tpiA/4186984‑4187276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A