Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,778	T→G	T779P (ACC→CCC)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,778	0	T	G	100.0%	183.4 / NA	52	T779P (ACC→CCC)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (23/29); total (23/29)

GCGTTTAACGCAGGATGTCTGTGAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGTGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGC  >  NC_000913/1784641‑1784914

gcgTTTAACGCAGGATGTCTGTGAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGGTTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGgggt                                                                                                                                        >  1:415575/1‑140 (MQ=255)

 cgTTTAACGCAGGATGTCTGTGAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTc                                                                                                                                       <  1:955467/140‑1 (MQ=255)

  gTTTAACGCAGGATGTCTGTGAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTcc                                                                                                                                      <  2:1132278/140‑1 (MQ=255)

       aCGCAGGATGTCTGTGAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGtt                                                                                                                                 <  1:990719/140‑1 (MQ=255)

                 tCTGTGAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAgg                                                                                                                       >  1:112071/1‑140 (MQ=255)

                     tgAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTAt                                                                                                                   <  2:222440/140‑1 (MQ=255)

                         gagaTTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGAt                                                                                                               <  1:888496/140‑1 (MQ=255)

                          agaTTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATc                                                                                                              >  1:29912/1‑140 (MQ=255)

                           gaTTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATcc                                                                                                             >  2:95997/1‑140 (MQ=255)

                           gaTTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATcc                                                                                                             <  1:359227/140‑1 (MQ=255)

                            aTTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAgaga                                                                                                                           >  1:320068/1‑125 (MQ=255)

                            aTTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAgaga                                                                                                                           <  2:320068/125‑1 (MQ=255)

                            aTTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATccc                                                                                                            >  2:551739/1‑140 (MQ=255)

                              tGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCct                                                                                                          <  2:991821/140‑1 (MQ=255)

                                       ttCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCa                                                                                                 >  1:182969/1‑140 (MQ=255)

                                        tCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCaa                                                                                                <  2:112071/140‑1 (MQ=255)

                                          aTCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCct                                                                                                          >  1:1046643/1‑128 (MQ=255)

                                          aTCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCct                                                                                                          <  2:1046643/128‑1 (MQ=255)

                                          aTCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAAcc                                                                                              >  2:682252/1‑140 (MQ=255)

                                          aTCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAAcc                                                                                              >  2:96676/1‑140 (MQ=255)

                                                    aTCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCaaa                                                                                    <  2:29912/140‑1 (MQ=255)

                                                    aTCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCaaa                                                                                    <  1:930965/140‑1 (MQ=255)

                                                      cacaTAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGt                                                                                  >  2:773558/1‑140 (MQ=255)

                                                                cGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTc                                                                        >  2:498207/1‑140 (MQ=255)

                                                                cGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTc                                                                        >  1:964372/1‑140 (MQ=255)

                                                                   cGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTcgga                                                                     <  2:182969/140‑1 (MQ=255)

                                                                   cGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTcgga                                                                     <  2:925980/140‑1 (MQ=255)

                                                                   cGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTcgga                                                                     <  2:998553/140‑1 (MQ=255)

                                                                   cGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTcgga                                                                     <  1:682252/140‑1 (MQ=255)

                                                                     aCTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTcggacg                                                                   <  1:627387/140‑1 (MQ=255)

                                                                           cgccgcCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCt                                                             <  1:699697/140‑1 (MQ=255)

                                                                               gccgGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGAcc                                                         >  2:526606/1‑140 (MQ=255)

                                                                                ccgGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAATCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCg                                                        >  2:139237/1‑140 (MQ=255)

                                                                                ccgGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCg                                                        <  1:773558/140‑1 (MQ=255)

                                                                                ccgGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCg                                                        >  1:536796/1‑140 (MQ=255)

                                                                                  ggggATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAATCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCa                                                      <  1:139237/140‑1 (MQ=255)

                                                                                   gggATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCaa                                                     <  1:397172/140‑1 (MQ=255)

                                                                                     gATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAAt                                                   <  1:188567/140‑1 (MQ=255)

                                                                                         tattttatttCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCg                                               <  2:964372/140‑1 (MQ=255)

                                                                                              tatttCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACata                                          >  2:112958/1‑140 (MQ=255)

                                                                                                     tcagttcagCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGccc                                   >  2:275308/1‑140 (MQ=255)

                                                                                                           cagCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTttc                             >  2:70966/1‑140 (MQ=255)

                                                                                                             gCCAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTttctt                           <  1:1077849/140‑1 (MQ=255)

                                                                                                               cAGGCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTcg                         <  1:70966/140‑1 (MQ=255)

                                                                                                                  gCTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCgg                      <  1:96676/140‑1 (MQ=255)

                                                                                                                   cTTAACCAGGTTTGCACCACGGGGTCCGGGTTCAGAGACATGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGc                     >  1:932568/1‑140 (MQ=255)

                                                                                                                            gTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGc            >  2:564442/1‑140 (MQ=255)

                                                                                                                            gTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGc            >  1:661074/1‑140 (MQ=255)

                                                                                                                            gTTTGCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGc            >  1:26898/1‑140 (MQ=255)

                                                                                                                                gCACCACGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATc        >  2:375878/1‑140 (MQ=255)

                                                                                                                                  accacGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGa      <  2:661074/140‑1 (MQ=255)

                                                                                                                                    cacGGGGTCCGGGTTCAGAGACATGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGAca    <  2:932568/140‑1 (MQ=255)

                                                                                                                                    cacGGGGTCCGGGTTCAGAGACAGGGTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGAca    <  2:246725/140‑1 (MQ=255)

                                                                                                                                      cGGGGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGAcagc  <  2:415575/140‑1 (MQ=255)

GCGTTTAACGCAGGATGTCTGTGAAGAGATTGAATAAGTTTCATCTTCGGGGATCACATAACCCCGGCGACTAAACGCCGCCGGGGATTTATTTTATTTCTTCAGTTCAGCCAGGCTTAACCAGGTTTGCACCACGGTGTCCGGGTTCAGAGACAGGCTATCGATCCCCTCTTCCATCAACCATGCGGCAAAGTCTTCGTGGTCGGACGGACCCTGACCGCAAATCCCGACATATTTGCCCTGTTTCTTCGCGGCACGGATAGCCATCGACAGC  >  NC_000913/1784641‑1784914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A