Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,758,213	T→C	G171G (GGA→GGG)	ldtE ←	L,D‑transpeptidase LdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,758,213	0	T	C	100.0%	166.6 / NA	48	G171G (GGA→GGG)	ldtE	L,D‑transpeptidase LdtE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/23); total (25/23)

CCGGTGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGTCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTG  >  NC_000913/1758080‑1758347

ccGGTGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCg                                                                                                                                     >  2:520762/1‑137 (MQ=255)

ccGGTGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCg                                                                                                                                     <  1:520762/137‑1 (MQ=255)

 cGGTGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAg                                                                                                                                 >  1:266494/1‑140 (MQ=255)

    tGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGaa                                                                                                                              >  2:381059/1‑140 (MQ=255)

    tGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGaa                                                                                                                              >  2:230455/1‑140 (MQ=255)

       aTTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGa                                                                                                                           >  1:428068/1‑140 (MQ=255)

                  tACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGGAAt                                                                                                                >  2:372035/1‑140 (MQ=255)

                     acCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTa                                                                                                             >  2:525937/1‑140 (MQ=255)

                      cccTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTaa                                                                                                            <  1:725239/140‑1 (MQ=255)

                       ccTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAAt                                                                                                           >  2:102976/1‑140 (MQ=255)

                       ccTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGGAATTTAAt                                                                                                           >  2:469833/1‑140 (MQ=255)

                             cTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTGCCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACg                                                                                                     <  2:56047/140‑1 (MQ=255)

                                aCGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTc                                                                                                  >  1:535977/1‑140 (MQ=255)

                                                   ccGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAAtg                                                                               <  1:414162/140‑1 (MQ=255)

                                                   ccGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAAtg                                                                               <  1:469833/140‑1 (MQ=255)

                                                    cGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAAtgc                                                                              >  2:443177/1‑140 (MQ=255)

                                                           cTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGt                                                                       <  1:102976/140‑1 (MQ=255)

                                                           cTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGt                                                                       <  2:535977/140‑1 (MQ=255)

                                                            tGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGt                                                                       >  2:382766/1‑139 (MQ=255)

                                                            tGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGt                                                                       <  1:382766/139‑1 (MQ=255)

                                                            tGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTa                                                                      <  2:405496/140‑1 (MQ=255)

                                                             ggTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAg                                                                     <  2:428068/140‑1 (MQ=255)

                                                                 ccATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGTAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGt                                                                 <  1:372035/140‑1 (MQ=255)

                                                                  cATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTc                                                                <  1:381059/140‑1 (MQ=255)

                                                                      aatgaGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAgg                                                            <  2:429372/140‑1 (MQ=255)

                                                                      aatgaGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAgg                                                            >  2:45688/1‑140 (MQ=255)

                                                                      aatgaGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAgg                                                            >  2:298471/1‑140 (MQ=255)

                                                                      aatgaGGTATTCGCCATTACCATGCGCGAAGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAgg                                                            >  2:549648/1‑140 (MQ=255)

                                                                        tgaGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGtt                                                          >  2:619560/1‑140 (MQ=255)

                                                                        tgaGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGtt                                                          <  1:212499/140‑1 (MQ=255)

                                                                                    cATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGGTAGGGAtt                                              >  2:454192/1‑140 (MQ=255)

                                                                                    cATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCACGGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGGtt                                              >  2:332345/1‑140 (MQ=255)

                                                                                      ttACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGAtttt                                            >  2:251753/1‑140 (MQ=255)

                                                                                       tACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGGTGGGGTAGGGAttttt                                           >  2:646691/1‑139 (MQ=255)

                                                                                         ccATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTg                                         <  1:597097/140‑1 (MQ=255)

                                                                                         ccATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTg                                         <  1:577113/140‑1 (MQ=255)

                                                                                            tGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTg                                         >  2:766088/1‑137 (MQ=255)

                                                                                            tGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTg                                         <  1:766088/137‑1 (MQ=255)

                                                                                             gcgcGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCa                                     >  1:23267/1‑140 (MQ=255)

                                                                                              cgcgAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCaa                                    <  1:646691/140‑1 (MQ=255)

                                                                                              cgcgAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCaa                                    <  1:298471/140‑1 (MQ=255)

                                                                                               gcgAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAac                                   <  1:45688/140‑1 (MQ=255)

                                                                                                  aGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTTATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGGTAGGGGTTTTTTGCCCAacacc                                >  2:597097/1‑139 (MQ=255)

                                                                                                    gcgcAGTGCGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACAcgcg                              >  1:259259/1‑140 (MQ=255)

                                                                                                            cGTAACGTCCTAGCGGGTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCCGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATc                      >  2:468075/1‑140 (MQ=255)

                                                                                                                          gggTTATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAg        <  1:20532/140‑1 (MQ=255)

                                                                                                                             ttATTTGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGccc     <  2:23267/140‑1 (MQ=255)

                                                                                                                                tttGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTg  <  1:619560/140‑1 (MQ=255)

                                                                                                                                tttGGCCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCCTTTCCAGCCCCTg  <  1:251753/140‑1 (MQ=255)

CCGGTGCATTCATGCGAATACACCCTGAACTGACGCGCAAACCGACGCTGTCCGGCGCACTGGTACCATGAATGAGGTATTCGCCATTACCATGCGCGAGGCGCAGTGCGTAACGTCCTAGCGGGTTATTTGGTCCGGCAGGAACGACTGGCGGTAATTTAATGCCACGCTCCAGCGAACGCTGACGAATGCCTGCCGTAGGCGTCCAGGTTGGGTTAGGGATTTTCTGCCCAACACGCGTTTCCATCACCGGCGTTTCCAGCCCCTG  >  NC_000913/1758080‑1758347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A