Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,761,634	G→A	P53L (CCG→CTG)	sufD ←	Fe‑S cluster scaffold complex subunit SufD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,761,634	0	G	A	100.0%	161.8 / NA	46	P53L (CCG→CTG)	sufD	Fe‑S cluster scaffold complex subunit SufD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/17); total (29/17)

CGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGTTA  >  NC_000913/1761497‑1761768

cGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCagcagc                                                                                                                                      >  1:339404/1‑140 (MQ=255)

 gCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcagcg                                                                                                                                     <  2:1055070/140‑1 (MQ=255)

       tGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATAtt                                                                                                                               <  1:761439/140‑1 (MQ=255)

         cGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTc                                                                                                                             >  1:823511/1‑140 (MQ=255)

               cGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGttt                                                                                                                       <  1:955275/140‑1 (MQ=255)

                 tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcagcg                                                                                                                                     >  2:660777/1‑124 (MQ=255)

                 tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcagcg                                                                                                                                     <  1:660777/124‑1 (MQ=255)

                 tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTc                                                                                                                     >  2:614828/1‑140 (MQ=255)

                 tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTc                                                                                                                     >  1:588227/1‑140 (MQ=255)

                 tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGGGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTc                                                                                                                     >  2:998607/1‑140 (MQ=255)

                      cccATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGtt                                                                                                                <  1:278973/140‑1 (MQ=255)

                        cATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTa                                                                                                              <  2:453646/140‑1 (MQ=255)

                          tCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACg                                                                                                            >  1:703901/1‑140 (MQ=255)

                          tCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACg                                                                                                            >  1:445875/1‑140 (MQ=255)

                                aaCACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCgg                                                                                                      <  2:588227/140‑1 (MQ=255)

                                  cacCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCa                                                                                                    <  2:1091989/140‑1 (MQ=255)

                                     cAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTc                                                                                                 >  2:984345/1‑140 (MQ=255)

                                     cAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGCGATATCTCTCCCGCAATGCTGACAAACTCGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTc                                                                                                 >  2:457281/1‑140 (MQ=255)

                                         cGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGt                                                                                             <  1:324229/140‑1 (MQ=255)

                                           cACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTAc                                                                                           >  2:11173/1‑140 (MQ=255)

                                                          tAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTAc                                                                                           <  2:482375/125‑1 (MQ=255)

                                                          tAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTAc                                                                                           >  1:482375/1‑125 (MQ=255)

                                                             cGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAAt                                                                         <  2:703901/140‑1 (MQ=255)

                                                                 aaGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAAtgctg                                                                     >  2:749310/1‑140 (MQ=255)

                                                                  aGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAAtgctgc                                                                    <  2:396863/140‑1 (MQ=255)

                                                                              gctgTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCggg                                                        >  1:756931/1‑140 (MQ=255)

                                                                                         atCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGt                                             >  2:835939/1‑140 (MQ=255)

                                                                                         atCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGt                                             >  1:822141/1‑140 (MQ=255)

                                                                                                ccGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCa                                      >  2:297329/1‑140 (MQ=255)

                                                                                                ccGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCa                                      >  1:6471/1‑140 (MQ=255)

                                                                                                   cAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCt                                   <  2:822141/140‑1 (MQ=255)

                                                                                                     aTGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTc                                 >  2:561114/1‑140 (MQ=255)

                                                                                                      tGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCa                                >  1:118824/1‑140 (MQ=255)

                                                                                                      tGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCa                                <  1:749310/140‑1 (MQ=255)

                                                                                                        cTGACAAACTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCaaa                              >  1:449195/1‑140 (MQ=255)

                                                                                                                cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  2:595312/1‑140 (MQ=255)

                                                                                                                cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      <  1:297329/140‑1 (MQ=255)

                                                                                                                cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  2:680451/1‑140 (MQ=255)

                                                                                                                cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  2:52400/1‑140 (MQ=255)

                                                                                                                cTGGCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGAt                      >  1:873812/1‑140 (MQ=255)

                                                                                                                  ggCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGc                    >  2:629871/1‑140 (MQ=255)

                                                                                                                  ggCTATTGATCAGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGc                    <  1:998607/140‑1 (MQ=255)

                                                                                                                             aGCCTTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAg         >  2:42342/1‑140 (MQ=255)

                                                                                                                             aGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAg         >  1:602676/1‑140 (MQ=255)

                                                                                                                             aGCCCTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAg         >  2:757529/1‑140 (MQ=255)

                                                                                                                               cccTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAgcg       >  2:561662/1‑140 (MQ=255)

                                                                                                                               cccTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAgcg       >  2:545721/1‑140 (MQ=255)

                                                                                                                               cccTTCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAgcg       <  2:873812/140‑1 (MQ=255)

                                                                                                                                  ttCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGt    <  1:757529/140‑1 (MQ=255)

                                                                                                                                  ttCCAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGt    <  2:602676/140‑1 (MQ=255)

                                                                                                                                    ccAGCAGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGTTa  >  2:1058888/1‑140 (MQ=255)

CGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTCATGTTTACGTGTCGGCAGTCCGGTACGCAGCAATTGTTGTAAATGCTGCTGTGCTTGCGGGGAGCGTTTTGTCCCTTCAGCTTCAAACAAGTGATGCCACTGTTGCAGCGCGTTA  >  NC_000913/1761497‑1761768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A