Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,446,311	T→C	S79G (AGC→GGC)	rpsH ←	30S ribosomal subunit protein S8

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,446,311	0	T	C	100.0%	150.8 / NA	41	S79G (AGC→GGC)	rpsH	30S ribosomal subunit protein S8
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/14); total (27/14)

CGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCTGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGCACGTTGGCGATTG  >  NC_000913/3446176‑3446442

cGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGaca                                                                                                                                 >  2:531475/1‑140 (MQ=255)

cGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGaca                                                                                                                                 >  1:98713/1‑140 (MQ=255)

                                        aaCACCTTTAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATAcc                                                                                         >  1:1061655/1‑139 (MQ=255)

                                          cacCTTTAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTc                                                                                       >  1:188520/1‑140 (MQ=255)

                                             cTTTAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCaga                                                                                    >  2:1072001/1‑140 (MQ=255)

                                              tttAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCagag                                                                                   >  2:437331/1‑140 (MQ=255)

                                                tAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTa                                                                                 >  2:653047/1‑140 (MQ=255)

                                                     gTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTc                                                                            >  1:688597/1‑140 (MQ=255)

                                                       aGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCa                                                                          >  2:592030/1‑140 (MQ=255)

                                                             aacTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAg                                                                    >  2:131807/1‑140 (MQ=255)

                                                                tGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCt                                                                 >  1:875451/1‑140 (MQ=255)

                                                                          gcccccGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGcc                                                       <  2:571141/137‑1 (MQ=255)

                                                                          aGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGtt                                                                       <  2:1054190/124‑1 (MQ=255)

                                                                          aGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGtt                                                                       >  1:1054190/1‑124 (MQ=255)

                                                                           gACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCt                                                      >  2:671221/1‑140 (MQ=255)

                                                                            tgccGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCtt                                                     <  2:27001/138‑1 (MQ=255)

                                                                            aCCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCtt                                                     <  1:131807/140‑1 (MQ=255)

                                                                             cccGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTc                                                    <  1:653047/140‑1 (MQ=255)

                                                                                 ccATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCt                                                                 <  1:119964/123‑1 (MQ=255)

                                                                                 ccATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCt                                                                 >  2:119964/1‑123 (MQ=255)

                                                                                 ccATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACt                                                >  1:527944/1‑140 (MQ=255)

                                                                                 ccATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACt                                                >  2:552865/1‑140 (MQ=255)

                                                                                 ccATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACt                                                >  1:331576/1‑140 (MQ=255)

                                                                                          tCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCt                                       <  1:671221/140‑1 (MQ=255)

                                                                                                 cTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATaa                                <  1:552865/140‑1 (MQ=255)

                                                                                                    aTCTTTACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAAc                             >  2:887227/1‑140 (MQ=255)

                                                                                                         tACGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACcttct                        <  2:471639/140‑1 (MQ=255)

                                                                                                           cGTTTATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCGATAAAACCttcttc                      >  2:287469/1‑140 (MQ=255)

                                                                                                              ttATAGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTcttcctt                   >  1:1070712/1‑140 (MQ=255)

                                                                                                                  aGATGCGCAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGc               <  1:531475/140‑1 (MQ=255)

                                                                                                                      gcgcAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGc               >  1:75850/1‑136 (MQ=255)

                                                                                                                      gcgcAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGc               <  2:75850/136‑1 (MQ=255)

                                                                                                                       cgcAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGc               <  2:657510/135‑1 (MQ=255)

                                                                                                                       cgcAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGc               >  1:657510/1‑135 (MQ=255)

                                                                                                                       cgcAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGCACGtt          >  2:246590/1‑140 (MQ=255)

                                                                                                                       cgcAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGCACGtt          >  1:236544/1‑140 (MQ=255)

                                                                                                                         cAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACcttctt                       <  2:665769/125‑1 (MQ=255)

                                                                                                                         cAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACcttctt                       >  1:665769/1‑125 (MQ=255)

                                                                                                                         cAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGc               <  2:395742/133‑1 (MQ=255)

                                                                                                                         cAGACCTGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGc               >  1:395742/1‑133 (MQ=255)

                                                                                                                               tGGGCGGCCGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGCACGTTGGCGATTg  >  2:740050/1‑140 (MQ=255)

CGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGTAGAAACAACTGCGATACCCAGACCCGCCATAACTTTCGGCAGCTCATCTTTACGTTTATAGATGCGCAGACCTGGGCGGCTGACACGCTGAATGCTTTCTACAACAGCTTTGCCCTGGAAATACTTCAGAGTAAGTTCCAGTTCAGGCTTGGTGTCGCCTTCAACTTTAAAATCTTCAATAAAACCTTCTTCCTTCAGCACGTTGGCGATTG  >  NC_000913/3446176‑3446442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A