Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,746,411	G→A	intergenic (‑284/‑24)	ydhQ ← / → valV	adhesin‑like autotransporter YdhQ/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,746,411	0	G	A	100.0%	121.7 / NA	36	intergenic (‑284/‑24)	ydhQ/valV	adhesin‑like autotransporter YdhQ/tRNA‑Val
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/20); total (16/20)

GATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAG  >  NC_000913/1746278‑1746540

gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  2:400448/1‑140 (MQ=255)

gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  2:381198/1‑140 (MQ=255)

gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  2:192339/1‑140 (MQ=255)

gATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCg                                                                                                                             >  1:738256/1‑140 (MQ=255)

 aTCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGt                                                                                                                            >  2:681674/1‑140 (MQ=255)

   ccTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTg                                                                                                                          <  1:192339/140‑1 (MQ=255)

   ccTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTg                                                                                                                          <  1:400448/140‑1 (MQ=255)

    cTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGt                                                                                                                         <  2:380116/140‑1 (MQ=255)

            aaaTCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACa                                                                                                                 >  1:165169/1‑140 (MQ=255)

              aTCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAAc                                                                                                               <  1:250178/140‑1 (MQ=255)

              aTCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAAc                                                                                                               <  2:65038/140‑1 (MQ=255)

                 aCCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGtt                                                                                                            >  1:286800/1‑140 (MQ=255)

                  cccTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTg                                                                                                           <  2:165169/140‑1 (MQ=255)

                      tGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGtt                                                                                                       >  2:665145/1‑140 (MQ=255)

                             caacaaAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTCGCGTTCATAGCt                                                                                                <  2:316245/140‑1 (MQ=255)

                                     tataGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        <  1:381198/140‑1 (MQ=255)

                                     tataGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        >  2:431915/1‑140 (MQ=255)

                                          ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        >  1:709731/1‑135 (MQ=255)

                                          ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGt                                                                                        <  2:709731/135‑1 (MQ=255)

                                          ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTagag                                                                                   >  1:236516/1‑140 (MQ=255)

                                          ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTagag                                                                                   <  1:665145/140‑1 (MQ=255)

                                          ccAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTagag                                                                                   <  2:333816/140‑1 (MQ=255)

                                             aTTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAg                                                                                             >  2:375227/1‑127 (MQ=255)

                                             aTTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAg                                                                                             <  1:375227/127‑1 (MQ=255)

                                             aTTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGcac                                                                                <  2:224335/140‑1 (MQ=255)

                                               ttGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGcacca                                                                              <  2:286800/140‑1 (MQ=255)

                                                          ctctGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACAtgg                                                                   <  1:754790/140‑1 (MQ=255)

                                                           tctGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACAtggt                                                                  <  2:569304/140‑1 (MQ=255)

                                                              gCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTggg                                                               <  1:431915/140‑1 (MQ=255)

                                                                      tCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTg                                                       >  2:613814/1‑140 (MQ=255)

                                                                                      aaTTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGa                                       <  1:613814/140‑1 (MQ=255)

                                                                                                             cTTCCCACTCTGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGT‑GGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCt               <  1:357999/140‑1 (MQ=255)

                                                                                                                    ctctGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTg         >  2:157586/1‑140 (MQ=255)

                                                                                                                       tGCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGtt      >  2:256788/1‑140 (MQ=255)

                                                                                                                        gCCGCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTa     >  1:788916/1‑140 (MQ=255)

                                                                                                                           gCTAATATTCATCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTagag  <  2:530106/140‑1 (MQ=255)

GATCCTGAATAAAAATCACCCTTGCAAATCAACAAAATATAGCCAATTTGCTTAAAAGCTCTGCAAACGGTCGAAATAGCGCAGAAAATTACGTTTTGCCTCTTGCCACCTTCCCACTCTGCCGCTAATATTCGTCCCCGTTGTCACCTACAACGTTGCGTTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGAACGCACCATCCTGCGTCCGTAGCTCAGTTGGTTAGAG  >  NC_000913/1746278‑1746540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A