Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F19 I0 R1 34 56.7 1876034 94.8% 1778480 147.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 4,186,457 A→G 45.3% K398E (AAG→GAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,186,4570AG45.3% 16.3 / 63.3 55K398E (AAG→GAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (11/19);  new base G (13/12);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

TCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTA  >  NC_000913_3_pae_tpiA/4186313‑4186604
                                                                                                                                                 |                                                                                                                                                   
tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCgaagaa                                                                                                                                                 >  1:62917/1‑149 (MQ=255)
  cGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGgaaa                                                                                                                                               >  1:652635/1‑149 (MQ=255)
  cGGTCGTTCCTTAATCACCGTACGTCCCTACCTGCGTCCGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCCTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGaaaa                                                                                                                                               <  2:222399/149‑1 (MQ=255)
    gTCGTTCGGTAATCACCGCAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATg                                                                                                                                             <  1:358635/149‑1 (MQ=255)
     tCGCTCTGTAATCACCTTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATgg                                                                                                                                            <  2:197234/149‑1 (MQ=255)
      cGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGt                                                                                                                                           <  2:20838/149‑1 (MQ=255)
       gTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCAGTCGGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAAATGCGTGGTCTTGCTACGCACC‑TTAAAGCTGCGACGaaaaaagtt                                                                                                                                          >  2:855621/1‑144 (MQ=255)
           tGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGacc                                                                                                                                      >  2:203022/1‑147 (MQ=255)
             tAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAAAAAATGGTTGAgcgc                                                                                                                                    >  1:383916/1‑149 (MQ=255)
              caTCACCGTATGTCCATACCTGTGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAAACGTCCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTTAGcgcg                                                                                                                                   <  2:467933/148‑1 (MQ=255)
                tCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCgaa                                                                                                                                 >  1:301769/1‑149 (MQ=255)
                   ccGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCgaagaa                                                                                                                              <  1:218679/149‑1 (MQ=255)
                    cGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAACCTGCGGAGAAAATGATTGAGCGCaaagagg                                                                                                                             >  1:796881/1‑147 (MQ=255)
                        ggTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGt                                                                                                                         <  1:179720/149‑1 (MQ=255)
                         gTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCCCCCCGGTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTc                                                                                                                        <  1:222739/149‑1 (MQ=255)
                         gTCAATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGACAATGGTTGAGCGCAAAGAAGCTGTc                                                                                                                        >  1:594244/1‑149 (MQ=255)
                              tACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTg                                                                                                                   <  2:578078/149‑1 (MQ=255)
                                 cTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGCGCTCTTCGGCC‑CACCAGTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                                                                                >  1:221149/1‑149 (MQ=255)
                                 cTGCGGCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCCCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTATAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                                                                                <  2:173894/149‑1 (MQ=255)
                                       cTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCt                                                                                                          <  2:855545/149‑1 (MQ=255)
                                        tGCATCACTGCGGGCGGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCAAC‑AACAATAAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTg                                                                                                         <  1:184408/149‑1 (MQ=255)
                                                          cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGaa                                                                                       >  1:850513/1‑149 (MQ=255)
                                                            aagaaAATGGCACTTGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAaca                                                                                     >  1:561866/1‑149 (MQ=255)
                                                              gaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                                                   <  1:870909/149‑1 (MQ=255)
                                                                 aaTGGCACTGGGGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATGCGCGAACACCCgg                                                                                <  1:631860/149‑1 (MQ=255)
                                                                 aaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                <  2:675495/149‑1 (MQ=255)
                                                                 aaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGATCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                                >  2:538708/1‑149 (MQ=255)
                                                                   tGGCCCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTa                                                                              <  2:537710/149‑1 (MQ=255)
                                                                       aCTGTAGCTGTTCAAACCGTTAATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGAActgc                                                                          >  2:349344/1‑149 (MQ=255)
                                                                                 ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGc                                                                >  2:660556/1‑149 (MQ=255)
                                                                                   cAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                                              <  1:660556/149‑1 (MQ=255)
                                                                                    aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                                             >  1:845917/1‑149 (MQ=255)
                                                                                    aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                                             >  1:211944/1‑149 (MQ=255)
                                                                                    aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACTAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                                             <  2:628546/149‑1 (MQ=255)
                                                                                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCg                                                            >  1:841387/1‑149 (MQ=255)
                                                                                             acgtACGGCAAGTGGGAACTGCGTGATACTTCTAC‑CATCATTAACGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGCGATATCCTGCACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                                                    <  2:847210/146‑1 (MQ=255)
                                                                                                tACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCg                                                 >  1:656980/1‑149 (MQ=255)
                                                                                                   ggCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt                                              >  1:707648/1‑149 (MQ=255)
                                                                                                     cagtcTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                            <  2:44792/145‑1 (MQ=255)
                                                                                                     cAAGTTGGAACTGCGTGGTCTTGCTAC‑CACGTTTAAAGCTTCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                            <  1:871321/149‑1 (MQ=255)
                                                                                                     cAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                            <  2:924149/149‑1 (MQ=255)
                                                                                                     cAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                            >  1:292631/1‑149 (MQ=255)
                                                                                                      aaGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTggg                                           <  2:794977/149‑1 (MQ=255)
                                                                                                          tGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATc                                       >  2:604231/1‑149 (MQ=255)
                                                                                                                tGCGTGGTCTTGCTAC‑TACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGTCTCTGCACCGTCTGGGTATCCAGGCa                                 <  1:535336/149‑1 (MQ=255)
                                                                                                                    tGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGACGCTGTCGTTTGGGATAGCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg                             <  1:774399/149‑1 (MQ=255)
                                                                                                                         ttGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTGCATTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGCCTGGGTATCCAGGCATTAGAACCg                        >  1:645683/1‑149 (MQ=255)
                                                                                                                         ttGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                            >  2:59129/1‑129 (MQ=255)
                                                                                                                         ttGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                            <  1:59129/129‑1 (MQ=255)
                                                                                                                         ttGCTAC‑CACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCg                        >  2:734588/1‑149 (MQ=255)
                                                                                                                            cTAC‑CACCATTAAAGCTGAGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGTAGTTATCCGCGAACACGCGGTACTGCTGAGCCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                     <  2:901093/149‑1 (MQ=255)
                                                                                                                                       tAAAGTTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGCCGTGTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg           <  2:128056/149‑1 (MQ=255)
                                                                                                                                            cTGCGGAGTAAATGGCTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGTACGAATTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCCCCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaaa      <  2:106283/149‑1 (MQ=255)
                                                                                                                                            cTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaaa      <  1:862836/149‑1 (MQ=255)
                                                                                                                                             tGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAg     <  2:483310/149‑1 (MQ=255)
                                                                                                                                              gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTAGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCCTTTGAACCGGTACTGCTCGAAGGTATAGc    >  2:534851/1‑149 (MQ=255)
                                                                                                                                                gaagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACGGATCGAAGGTAAAGCTa  >  2:447118/1‑149 (MQ=255)
                                                                                                                                                gaagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTGATTTTCTAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTa  >  1:271989/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTAC‑CACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTA  >  NC_000913_3_pae_tpiA/4186313‑4186604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A