Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F130 I1 R1	38	76.4	2725422	95.3%	2597327	137.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,158,984	G→T	M193I (ATG→ATT)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,158,984	0	G	T	98.8%	287.0 / ‑3.5	81	M193I (ATG→ATT)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (39/41); total (39/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTA  >  NC_000913_3_pae_tpiA/4158837‑4159122

gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCt                                                                                                                                          <  1:658082/150‑1 (MQ=255)

gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCt                                                                                                                                          <  1:658052/150‑1 (MQ=255)

gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCt                                                                                                                                          <  1:471911/150‑1 (MQ=255)

gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCt                                                                                                                                          >  3:202381/1‑150 (MQ=255)

 aTCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTg                                                                                                                                         >  2:338605/1‑150 (MQ=255)

  tCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTgg                                                                                                                                        >  1:761006/1‑150 (MQ=255)

     tCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCCATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAg                                                                                                                                     >  2:183742/1‑150 (MQ=255)

        cgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATg                                                                                                                                  <  3:376981/150‑1 (MQ=255)

          cTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGt                                                                                                                                >  4:202020/1‑150 (MQ=255)

          cTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCACGAGCGAACCTCGAATGCGTACCGATGGCCGAAAAAAAAAAAAAAAAACTGCTGATTCTGGAAGATGGt                                                                                                                                <  2:376707/150‑1 (MQ=255)

          cTGGTAAAGGAAAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATAACTCCATATTGCTAGCTTTTGATAAATATCACCCGTCCGCGAACCGCGAATGCGTAACGATGCCCGATCTGTCAGCGTCAAAACTTCTAATTCTGGAAGATGGt                                                                                                                                <  4:262249/150‑1 (MQ=255)

           tGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTc                                                                                                                               >  4:177551/1‑150 (MQ=255)

            ggTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCa                                                                                                                              <  1:299845/150‑1 (MQ=255)

              tGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACt                                                                                                                            <  4:315828/150‑1 (MQ=255)

               gAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTg                                                                                                                           >  1:89067/1‑150 (MQ=255)

                  agagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGttt                                                                                                                        <  3:266819/150‑1 (MQ=255)

                   gagagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTg                                                                                                                       >  1:214940/1‑150 (MQ=255)

                    agagCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTgc                                                                                                                      >  2:184701/1‑150 (MQ=255)

                      agCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTgcgc                                                                                                                    >  4:474428/1‑150 (MQ=255)

                      agCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCCATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTgcgc                                                                                                                    <  1:183742/150‑1 (MQ=255)

                                tcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                                          >  2:713680/1‑150 (MQ=255)

                                tcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                                          <  2:118511/150‑1 (MQ=255)

                                       aGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                                   <  2:764189/150‑1 (MQ=255)

                                       aGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                                   <  2:710594/150‑1 (MQ=255)

                                           ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                                                <  4:525672/133‑1 (MQ=255)

                                           ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                                                >  3:525672/1‑133 (MQ=255)

                                            cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt                                                                                              <  3:177551/150‑1 (MQ=255)

                                             gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCttctg                                                                                             >  1:385568/1‑146 (MQ=255)

                                             gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGttt                                                                                             <  1:338605/150‑1 (MQ=255)

                                             gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGttt                                                                                             <  1:498633/150‑1 (MQ=255)

                                              ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                            <  1:91102/150‑1 (MQ=255)

                                              ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                            <  2:89067/150‑1 (MQ=255)

                                              ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                            <  2:761006/150‑1 (MQ=255)

                                              ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                            <  1:352804/150‑1 (MQ=255)

                                                 tttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGaa                                                                                         <  3:129995/150‑1 (MQ=255)

                                                     atgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                                     >  2:16752/1‑150 (MQ=255)

                                                     atgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCg                                                                                     <  2:322792/150‑1 (MQ=255)

                                                          ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                                   <  1:731284/131‑1 (MQ=255)

                                                          ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                                   >  2:731284/1‑131 (MQ=255)

                                                            aaTGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                                              >  4:440353/1‑150 (MQ=255)

                                                              tGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                                   >  3:518692/1‑143 (MQ=255)

                                                              tGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                                   <  4:518692/143‑1 (MQ=255)

                                                              tGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCTGGGCGGATg                                                                            <  3:278378/150‑1 (MQ=255)

                                                                    tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                      <  3:73824/150‑1 (MQ=255)

                                                                    tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                      <  1:782600/150‑1 (MQ=255)

                                                                        tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGACGCCGGGGCGGATGAAGATacaca                                                                  >  2:375231/1‑150 (MQ=255)

                                                                         atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                 <  2:698672/150‑1 (MQ=255)

                                                                         atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                 <  1:28657/150‑1 (MQ=255)

                                                                         atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                 <  1:28651/150‑1 (MQ=255)

                                                                         atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                 >  1:452518/1‑150 (MQ=255)

                                                                             atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTg                                                                                           <  2:444732/120‑1 (MQ=255)

                                                                             atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTg                                                                                           >  1:444732/1‑120 (MQ=255)

                                                                             atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc                                                             >  2:678680/1‑150 (MQ=255)

                                                                              tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                            >  3:474022/1‑150 (MQ=255)

                                                                              tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                            >  2:150657/1‑150 (MQ=255)

                                                                                 aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcgc                                                         >  3:26742/1‑150 (MQ=255)

                                                                                   aTCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTGCTGTTTTGAGGCCGGGGGGGCTGAAGATACAGACTTCCCGGCGa                                                       >  2:274092/1‑150 (MQ=255)

                                                                                      aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCa                                                    >  3:123542/1‑150 (MQ=255)

                                                                                      aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCa                                                    >  2:26389/1‑150 (MQ=255)

                                                                                        ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa                                                       >  1:190040/1‑145 (MQ=255)

                                                                                        ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGa                                                       <  2:190040/145‑1 (MQ=255)

                                                                                         cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                 <  3:228387/134‑1 (MQ=255)

                                                                                         cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                 >  4:228387/1‑134 (MQ=255)

                                                                                             ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGa                                             <  2:452518/150‑1 (MQ=255)

                                                                                              gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaa                                            <  1:812549/150‑1 (MQ=255)

                                                                                                   ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct                                       >  4:119980/1‑150 (MQ=255)

                                                                                                      cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                                 <  4:289452/73‑1 (MQ=255)

                                                                                                      cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                                 >  3:289452/1‑73 (MQ=255)

                                                                                                          tGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAAc                                <  4:193262/150‑1 (MQ=255)

                                                                                                                  gATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggc                        >  3:485326/1‑150 (MQ=255)

                                                                                                                  gATGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggc                        >  2:104705/1‑150 (MQ=255)

                                                                                                                    tGGCCGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                      <  1:123345/150‑1 (MQ=255)

                                                                                                                       ccGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                   <  4:123542/150‑1 (MQ=255)

                                                                                                                        cGATCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAgg                  >  4:309552/1‑150 (MQ=255)

                                                                                                                           tCTGGCAGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAg               >  4:435606/1‑150 (MQ=255)

                                                                                                                                 aGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt         >  2:392550/1‑150 (MQ=255)

                                                                                                                                 aGGGGAAAAACTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGAACAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt         >  4:47001/1‑150 (MQ=255)

                                                                                                                                     gAAAGACTGCTGATTGTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt     >  4:237451/1‑150 (MQ=255)

                                                                                                                                        aaaCTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt     >  4:114623/1‑147 (MQ=255)

                                                                                                                                        aaaCTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt     <  3:114623/147‑1 (MQ=255)

                                                                                                                                        aaaCTGCTGATTCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTa  <  4:202381/150‑1 (MQ=255)

GATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTA  >  NC_000913_3_pae_tpiA/4158837‑4159122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A