Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F151 I1 R1 22 137.2 5304824 94.1% 4991839 128.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,186,457 A→G K398E (AAG→GAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,186,4570AG100.0% 465.9 / NA 131K398E (AAG→GAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (68/63);  total (68/63)

Only 100 of 132 total aligned reads displayed.

ACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCT  >  NC_000913_3_pae_tpiA/4186311‑4186603
                                                                                                                                                  |                                                                                                                                                  
actCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCggaga                                                                                                                                                 >  3:940638/1‑150 (MQ=255)
  tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACCGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGaaa                                                                                                                                               >  2:50207/1‑150 (MQ=255)
    cGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAAt                                                                                                                                             <  1:159866/150‑1 (MQ=255)
      gTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATgg                                                                                                                                           <  4:125874/150‑1 (MQ=255)
       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGt                                                                                                                                          >  4:278726/1‑150 (MQ=255)
       tCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGt                                                                                                                                          <  4:574511/150‑1 (MQ=255)
        cGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         >  3:777073/1‑150 (MQ=255)
        cGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCCTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         <  1:795156/150‑1 (MQ=255)
        cGTTCTGTAATCACCGTAGGCCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGTGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         <  1:226949/150‑1 (MQ=255)
          ttCTTTAATCACCGTAGGGCCATACCGGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATGAAAGCTGCGGAGAAAATGGTTGa                                                                                                                                       <  2:1062354/150‑1 (MQ=255)
            cTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAgc                                                                                                                                     >  2:1531297/1‑150 (MQ=255)
             tGTAATCACCGTAGGTCCATACCTGCGTCTGCGTCAGTGCGGTCGTCCGTACTAAATGGCCCTGGAGCTGTTCAAACCGTTCATCTCCGGCAACCCCCAACTGCGTGGTCTTGCTACCACCATTAAAGCCCCGGACACAATGGTTGAccg                                                                                                                                    >  2:897579/1‑150 (MQ=255)
               tAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGcgcg                                                                                                                                  >  1:1439571/1‑150 (MQ=255)
               tAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGcgcg                                                                                                                                  >  3:152671/1‑150 (MQ=255)
                  tCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCgaag                                                                                                                               <  4:777073/150‑1 (MQ=255)
                    aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCgaagaa                                                                                                                             >  1:101491/1‑150 (MQ=255)
                    aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCgaagaa                                                                                                                             >  1:454787/1‑150 (MQ=255)
                     ccGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGaagaag                                                                                                                            >  3:875467/1‑150 (MQ=255)
                      cGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                                           >  2:301403/1‑150 (MQ=255)
                        tAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTg                                                                                                                         >  1:1089233/1‑150 (MQ=255)
                          ggcgcATACCTGCGCCTGCACCAGCGCCGTCTGACGAAGAAAATGGCACTGGAGCTGTTCAAGCCGGTCAGCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACTATGAAGGATGCGGAGAAAATGGTTGAGCGCGAAGCAGCTGTc                                                                                                                       <  2:121822/146‑1 (MQ=255)
                          ggTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAAGGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTc                                                                                                                       <  1:15333/150‑1 (MQ=255)
                           gTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCTTCGAAAATGTCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCg                                                                                                                      >  1:794821/1‑150 (MQ=255)
                           gTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCg                                                                                                                      >  1:1332017/1‑150 (MQ=255)
                             ccATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGtt                                                                                                                    >  1:726789/1‑150 (MQ=255)
                              cATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                                                   <  2:794821/150‑1 (MQ=255)
                              cATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                                                   <  3:10020/150‑1 (MQ=255)
                                   cTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                                                   <  1:248266/145‑1 (MQ=255)
                                   cTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGttt                                                                                                                   >  2:248266/1‑145 (MQ=255)
                                   cTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                                 <  2:887805/147‑1 (MQ=255)
                                   cTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                                 >  1:887805/1‑147 (MQ=255)
                                            cATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGAc                                                                                                     >  1:1093312/1‑150 (MQ=255)
                                            cATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGAc                                                                                                     >  1:1470925/1‑150 (MQ=255)
                                             aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATgg                                                                                                                                           >  2:862625/1‑111 (MQ=255)
                                             aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATgg                                                                                                                                           <  1:862625/111‑1 (MQ=255)
                                             aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         >  2:479144/1‑113 (MQ=255)
                                               cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                  <  4:370731/150‑1 (MQ=255)
                                               cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                  <  2:1349766/150‑1 (MQ=255)
                                               cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                  <  3:47781/150‑1 (MQ=255)
                                                      gTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATcc                                                                                           <  1:301403/150‑1 (MQ=255)
                                                         tGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgcg                                                                                        >  3:434698/1‑150 (MQ=255)
                                                           ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGaa                                                                                      >  2:535794/1‑150 (MQ=255)
                                                           ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGaa                                                                                      <  4:875467/150‑1 (MQ=255)
                                                            cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGAAATCCTGGACGAAGTTATCCGCGAac                                                                                     >  1:1216178/1‑150 (MQ=255)
                                                                  aaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTCAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                               <  2:1216178/150‑1 (MQ=255)
                                                                             gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                                >  4:566207/1‑106 (MQ=255)
                                                                             gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                                 <  2:112397/105‑1 (MQ=255)
                                                                             gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                                 >  1:112397/1‑105 (MQ=255)
                                                                                cTGTTCAAACCGTTCATCTACGGCAAGCTGGGACTGCGTGGTCTTGCTACCACCATTCAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGt                                                                 >  4:1010818/1‑150 (MQ=255)
                                                                                cTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGa                                                                                                   <  1:575507/116‑1 (MQ=255)
                                                                                  gTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         <  3:660992/76‑1 (MQ=255)
                                                                                  gTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         >  4:660992/1‑76 (MQ=255)
                                                                                  gTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCgg                                                                               >  1:840170/1‑134 (MQ=255)
                                                                                  gTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTGATCCGCGAACACCCgg                                                                               <  2:840170/134‑1 (MQ=255)
                                                                                   ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCTAACACCCGGTACTGCTGAACCGTGCa                                                              >  2:1468147/1‑150 (MQ=255)
                                                                                     cAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                                            >  1:1194196/1‑150 (MQ=255)
                                                                                      aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCg                                                           >  3:299939/1‑150 (MQ=255)
                                                                                       aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGa                                                                                                               >  2:794805/1‑97 (MQ=255)
                                                                                        aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGAc                                                         >  4:932642/1‑150 (MQ=255)
                                                                                               aTCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                  >  2:379717/1‑150 (MQ=255)
                                                                                                  tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGt                                               >  4:39964/1‑150 (MQ=255)
                                                                                                     ggCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTg                                            <  2:1470925/150‑1 (MQ=255)
                                                                                                      gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGCAGTTATCGGCGGACCCCCGGGACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                           <  2:1093312/150‑1 (MQ=255)
                                                                                                      gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                           <  4:929903/150‑1 (MQ=255)
                                                                                                      gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                           >  3:30252/1‑150 (MQ=255)
                                                                                                            tGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                     >  1:359636/1‑150 (MQ=255)
                                                                                                             ggAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCa                                    >  2:1337391/1‑150 (MQ=255)
                                                                                                             ggAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCa                                    <  2:1194196/150‑1 (MQ=255)
                                                                                                             ggAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCa                                    <  4:434698/150‑1 (MQ=255)
                                                                                                                aCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgc                                                                         <  3:207037/110‑1 (MQ=255)
                                                                                                                aCTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgc                                                                         >  4:207037/1‑110 (MQ=255)
                                                                                                                  tGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt                               <  4:506833/150‑1 (MQ=255)
                                                                                                                  tGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt                               <  4:299939/150‑1 (MQ=255)
                                                                                                                    cGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                                                  <  1:205596/97‑1 (MQ=255)
                                                                                                                    cGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                                                  >  2:205596/1‑97 (MQ=255)
                                                                                                                     gTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         <  4:306905/41‑1 (MQ=255)
                                                                                                                     gTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                         >  3:306905/1‑41 (MQ=255)
                                                                                                                       ggTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgc                                                                                         >  2:578958/1‑87 (MQ=255)
                                                                                                                       ggTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgc                                                                                         <  1:578958/87‑1 (MQ=255)
                                                                                                                        gTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                                                   <  1:645982/124‑1 (MQ=255)
                                                                                                                        gTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa                                                   >  2:645982/1‑124 (MQ=255)
                                                                                                                         tCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTACTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATCTGAAcc                        >  1:877605/1‑150 (MQ=255)
                                                                                                                             gCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATcc                                                                                           >  2:638691/1‑79 (MQ=255)
                                                                                                                             gCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATcc                                                                                           <  1:638691/79‑1 (MQ=255)
                                                                                                                             gCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                    <  1:1531297/150‑1 (MQ=255)
                                                                                                                              cTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc                   <  1:50207/150‑1 (MQ=255)
                                                                                                                                  caccaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgc                                                                                         >  1:1146345/1‑76 (MQ=255)
                                                                                                                                  caccaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgc                                                                                         <  2:1146345/76‑1 (MQ=255)
                                                                                                                                   accaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                                            <  1:1173825/104‑1 (MQ=255)
                                                                                                                                   accaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc                                                            >  2:1173825/1‑104 (MQ=255)
                                                                                                                                    ccaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                              >  1:1096133/1‑101 (MQ=255)
                                                                                                                                    ccaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                              <  2:1096133/101‑1 (MQ=255)
                                                                                                                                       ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATTTCCTGGACGAAGTTATCCGCGAAAACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg          <  2:1332017/150‑1 (MQ=255)
                                                                                                                                       ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg          >  1:1389138/1‑150 (MQ=255)
                                                                                                                                       ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg          <  4:324506/150‑1 (MQ=255)
                                                                                                                                       ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg          >  1:633530/1‑150 (MQ=255)
                                                                                                                                         aaaGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCCAGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGt        <  3:492457/150‑1 (MQ=255)
                                                                                                                                           aGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaa      >  3:829474/1‑150 (MQ=255)
                                                                                                                                            gCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaaa     <  4:829474/150‑1 (MQ=255)
                                                                                                                                               gCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCt  >  4:282640/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCT  >  NC_000913_3_pae_tpiA/4186311‑4186603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A