Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F55 I0 R1 2523 96.7 4820918 92.8% 4473811 133.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,655,687 C→A 23.9% intergenic (‑91/+61) epd ← / ← tktA D‑erythrose 4‑phosphate dehydrogenase/transketolase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,655,6870CA23.9% 214.1 / 56.1 100intergenic (‑91/+61)epd/tktAD‑erythrose 4‑phosphate dehydrogenase/transketolase A
Reads supporting (aligned to +/- strand):  ref base C (43/33);  new base A (12/12);  total (55/45)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

CACAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGA  >  NC_002947/5655539‑5655834
                                                                                                                                                         |                                                                                                                                                    
cacaGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAATTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCa                                                                                                                                                     >  1:1369802/1‑150 (MQ=255)
      ggCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccct                                                                                                                                                <  2:776264/150‑3 (MQ=255)
      ggCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACctct                                                                                                                                               >  2:2023469/1‑150 (MQ=255)
       gCCGATACGGCCATAACCGTTGAGTGCAAATTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtg                                                                                                                                              <  2:660627/150‑1 (MQ=255)
        ccGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccctgt                                                                                                                                              <  2:255234/150‑5 (MQ=38)
         cGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑Ctgtg                                                                                                                                            <  2:1920663/150‑1 (MQ=38)
         cGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGG‑‑‑‑‑GCGGTGTCGCCTTCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTgg                                                                                                                                           <  2:622745/150‑1 (MQ=12)
          gATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg                                                                                                                                           >  2:212986/1‑150 (MQ=255)
          gATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGATCCCACAG‑GGTTTCATGCAACCTCTGTgg                                                                                                                                           <  1:445537/150‑1 (MQ=255)
           aTACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTggg                                                                                                                                          <  2:2235560/150‑1 (MQ=38)
           aTACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTggg                                                                                                                                          <  2:212734/150‑1 (MQ=38)
              cGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTNGCGGGCNAGCCCGCTCCCANAG‑GGTTTCATGCAACCTCTGTGGGAGc                                                                                                                                       <  1:1751516/150‑1 (MQ=255)
              cGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGc                                                                                                                                       <  2:844912/150‑1 (MQ=38)
              cGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGc                                                                                                                                       <  1:1921733/150‑1 (MQ=255)
                  cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt                                                                                                                                             >  1:40797/1‑140 (MQ=255)
                  cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt                                                                                                                                             <  2:40797/140‑1 (MQ=255)
                  cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGc                                                                                                                                   >  1:1334896/1‑150 (MQ=255)
                  cATAACCGTTGAGTACAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt                                                                                                                                             <  1:1258852/140‑1 (MQ=255)
                  cATAACCGTTGAGTACAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt                                                                                                                                             >  2:1258852/1‑140 (MQ=255)
                    tacccGTTGAGTGCAACTTTGTAGGGACGGTGGGGCTTAGTTATCTCGTGAAACATTTCAATTGTCGTCAGCATCCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCtt                                                                                                                                 <  1:2340989/147‑1 (MQ=37)
                        cGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGc‑cc                                                                                                                            >  2:1657276/1‑150 (MQ=255)
                          ttGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑Ccgc                                                                                                                          >  2:77435/1‑150 (MQ=255)
                               tGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCggg                                                                                                                                    <  1:1680347/136‑1 (MQ=255)
                               tGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCggg                                                                                                                                    >  2:1680347/1‑136 (MQ=255)
                                gCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTCGCCTTCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑Ctgtg                                                                                                                                            <  2:2120468/131‑1 (MQ=18)
                                gCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTCGCCTTCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑Ctgtg                                                                                                                                            >  1:2120468/1‑131 (MQ=18)
                                 cAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGaa                                                                                                                   <  1:2023469/150‑1 (MQ=255)
                                  aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAg                                                                                                                  <  1:2252942/150‑1 (MQ=255)
                                  aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCAAGC‑Ccgctcccaca                                                                                                                   >  2:2232156/1‑142 (MQ=39)
                                  aaCTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCAAGC‑Ccgctcccaca                                                                                                                   <  1:2232156/149‑8 (MQ=39)
                                    cTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGc                                                                                                                <  1:1773769/150‑1 (MQ=255)
                                     tttGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGc                                                                                                                <  1:1743802/149‑1 (MQ=255)
                                     tttGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGc                                                                                                                >  2:1743802/1‑149 (MQ=255)
                                         tAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGacac                                                                                                           >  1:509536/1‑150 (MQ=255)
                                         tAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTGCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGacac                                                                                                           >  1:147104/1‑150 (MQ=255)
                                                  tGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAg                                                                                                  >  1:112073/1‑150 (MQ=255)
                                                      gCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGc                                                                                                                <  2:1428887/132‑1 (MQ=255)
                                                      gCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGc                                                                                                                >  1:1428887/1‑132 (MQ=255)
                                                          tCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTaa                                                                                          <  1:1657276/150‑1 (MQ=255)
                                                             gggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATc                                                                                       >  2:330460/1‑150 (MQ=255)
                                                              ggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCa                                                                                      <  1:330460/150‑1 (MQ=255)
                                                                cTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCAACGCCAA‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGt                                                                                    >  1:126335/1‑150 (MQ=39)
                                                                 tCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTc                                                                                   >  2:2334596/1‑150 (MQ=255)
                                                                   aCGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGTATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCtt                                                                                 >  2:1442240/1‑150 (MQ=255)
                                                                   aCGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCtt                                                                                 >  1:2043524/1‑150 (MQ=255)
                                                                      agaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg                                                                                                                                           <  1:506475/90‑1 (MQ=255)
                                                                      agaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg                                                                                                                                           >  2:506475/1‑90 (MQ=255)
                                                                      agaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGa                                                                                                              >  1:1218188/1‑118 (MQ=255)
                                                                      agaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGa                                                                                                              <  2:1218188/118‑1 (MQ=255)
                                                                         cAGTTCAATTGGCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCcagca                                                                           <  1:1093970/150‑1 (MQ=255)
                                                                            ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTgg                                                                                                                                           >  1:400642/1‑84 (MQ=17)
                                                                            ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTgg                                                                                                                                           <  2:400642/84‑1 (MQ=17)
                                                                                aTTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTc                                                                    >  2:821375/1‑150 (MQ=255)
                                                                                 ttGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACAcc                                                                                                          >  1:1070474/1‑111 (MQ=255)
                                                                                 ttGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACAcc                                                                                                          <  2:1070474/111‑1 (MQ=255)
                                                                                            cTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                        <  2:112073/150‑1 (MQ=255)
                                                                                            cTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                        <  2:1334896/150‑1 (MQ=255)
                                                                                                tgAATGTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGAGCTGTAAATCAGTCTTCCATCAGGTCTTCGGCGGTGCCGGGGTTg                                                    >  1:953405/2‑150 (MQ=37)
                                                                                                        gtgtgtGG‑‑‑‑TATCGGCGGATAAGCCCGCGCCCACAG‑GGTTTCTTGCAAGATCTGTGGGAGCGGGCTTGC‑CGGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAg                                            <  2:386822/148‑1 (MQ=255)
                                                                                                               g‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAg                                     >  1:2011693/1‑150 (MQ=255)
                                                                                                                     aTTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGcc                                   >  1:750185/1‑150 (MQ=255)
                                                                                                                       tCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCaa                                 >  2:2010686/1‑150 (MQ=255)
                                                                                                                            ggCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCGGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCACCAGCTCTCCGGCACTACCGGGGATGTTCTCCAGGGCGACGCCAAActct                            >  2:1911092/1‑150 (MQ=38)
                                                                                                                             gCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTc                                                                                   >  2:1881790/1‑94 (MQ=255)
                                                                                                                             gCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTc                                                                                   <  1:1881790/94‑1 (MQ=255)
                                                                                                                             gCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCtt                           <  2:509536/150‑1 (MQ=255)
                                                                                                                              cAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc                          <  2:1369802/150‑1 (MQ=255)
                                                                                                                               aaGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCg                         >  2:1593990/1‑150 (MQ=255)
                                                                                                                                 gCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAgg                                                                                                                 >  2:2233566/1‑60 (MQ=255)
                                                                                                                                 gCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAgg                                                                                                                 <  1:2233566/60‑1 (MQ=255)
                                                                                                                                   ccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATTGGTAGATCAGTCTTCCAGCCGCTCTAACAGATCACTTGTGATGGTCTCCAGGGTGAAGCTAAACTCTTCGAACa                     >  1:1739306/1‑150 (MQ=12)
                                                                                                                                   ccGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCTAACa                     >  2:160006/1‑150 (MQ=255)
                                                                                                                                   ccGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACa                     >  2:1420437/1‑150 (MQ=255)
                                                                                                                                       tCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc                          >  2:988344/1‑141 (MQ=255)
                                                                                                                                       tCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc                          <  1:988344/141‑1 (MQ=255)
                                                                                                                                        cccACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGcc                >  1:153814/1‑150 (MQ=255)
                                                                                                                                         ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCttt                                                                                            <  1:1947414/73‑1 (MQ=14)
                                                                                                                                         ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCttt                                                                                            >  2:1947414/1‑73 (MQ=14)
                                                                                                                                         ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTaaa                                                                                         <  1:44024/76‑1 (MQ=14)
                                                                                                                                         ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTaaa                                                                                         >  2:44024/1‑76 (MQ=14)
                                                                                                                                         ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCGAACTCTTCGAACAATGCCg               >  2:1587301/1‑150 (MQ=38)
                                                                                                                                         ccACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCg               >  1:2138817/1‑150 (MQ=38)
                                                                                                                                          cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGa              >  1:198810/7‑150 (MQ=38)
                                                                                                                                          cacagaggTTGCATGAAACC‑CTGTGAGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTCCGAACAATGCCGa              >  1:2199618/7‑150 (MQ=38)
                                                                                                                                          cacaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGa                                                      <  2:1895043/110‑1 (MQ=255)
                                                                                                                                          cacaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGa                                                      >  1:1895043/1‑110 (MQ=255)
                                                                                                                                           acagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTtctc                                               <  2:1900557/111‑1 (MQ=25)
                                                                                                                                           acagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTtctc                                               >  1:1900557/6‑116 (MQ=25)
                                                                                                                                           acagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGaa             >  2:2019248/6‑150 (MQ=38)
                                                                                                                                            cagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAg            >  1:1712909/5‑150 (MQ=38)
                                                                                                                                            caG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAgg                                           <  2:710316/119‑1 (MQ=255)
                                                                                                                                            caG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAgg                                           >  1:710316/1‑119 (MQ=255)
                                                                                                                                            caG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAg            >  2:2191220/1‑150 (MQ=255)
                                                                                                                                                  tttCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGa                                                         <  2:2207013/100‑1 (MQ=255)
                                                                                                                                                  tttCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGa                                                         >  1:2207013/1‑100 (MQ=255)
                                                                                                                                                  tttCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGgc       >  2:1678559/1‑150 (MQ=255)
                                                                                                                                                  ttgcATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCGTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAACCTCCTCGAACAATGCCGAAGCCGgc       >  1:85252/4‑150 (MQ=255)
                                                                                                                                                    tCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGgcgc     <  1:212986/150‑1 (MQ=255)
                                                                                                                                                       tGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGa  <  1:1678559/150‑1 (MQ=255)
                                                                                                                                                       tGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGa  >  2:917550/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                                    
CACAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGG‑‑‑‑CATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGC‑CCGCGAATGAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCGA  >  NC_002947/5655539‑5655834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A