Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F55 I0 R1 2523 96.7 4820918 92.8% 4473811 133.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,701,285 G→C 5.5% intergenic (+27/‑36) phaA → / → phaB poly(3‑hydroxyalkanoate) polymerase/poly(3‑hydroxyalkanoate) depolymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,701,2850GC 5.5% 345.5 / 8.3 109intergenic (+27/‑36)phaA/phaBpoly(3‑hydroxyalkanoate) polymerase/poly(3‑hydroxyalkanoate) depolymerase
Reads supporting (aligned to +/- strand):  ref base G (47/56);  new base C (3/3);  total (50/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 114 total aligned reads displayed.

TTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCATCGGC  >  NC_002947/5701136‑5701434
                                                                                                                                                     |                                                                                                                                                      
ttGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACgg                                                                                                                                                        >  2:2175314/1‑150 (MQ=255)
    aCTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGcc                                                                                                                                                    >  2:491501/1‑150 (MQ=255)
      tGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCTACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCAc                                                                                                                                                  <  1:1235455/150‑1 (MQ=255)
      tGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCAc                                                                                                                                                  <  2:494367/150‑1 (MQ=255)
      tGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCAc                                                                                                                                                  >  1:470773/1‑150 (MQ=255)
        gCAGGGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACgg                                                                                                                                                >  1:1627516/1‑150 (MQ=255)
        gCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCCGGCCCCTACGTTCACGAGCCTCGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCAccg                                                                                                                                                >  2:741771/1‑148 (MQ=255)
          agagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgtg                                                                                                                                              >  2:1194939/1‑150 (MQ=255)
           gagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgtgt                                                                                                                                             <  2:2179166/150‑1 (MQ=255)
           gagTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGgtgt                                                                                                                                             >  1:1648082/1‑150 (MQ=255)
            agTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGtt                                                                                                                                            <  2:671311/150‑1 (MQ=255)
              ttGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTa                                                                                                                                          <  1:491501/150‑1 (MQ=255)
              ttGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTa                                                                                                                                          <  1:1086051/150‑1 (MQ=255)
                ggCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACt                                                                                                                                        <  1:386026/150‑1 (MQ=255)
                    gggCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAc                                                                                                                                    <  1:1083296/150‑1 (MQ=255)
                        gAGCGTGCCGGCGCGCTGACAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGGGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa                                                                                                                                <  2:163636/150‑1 (MQ=255)
                        gAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa                                                                                                                                >  2:2355462/1‑150 (MQ=255)
                         aGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAag                                                                                                                               <  1:1358019/150‑1 (MQ=255)
                          gCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAag‑a                                                                                                                             <  1:1194939/150‑1 (MQ=255)
                              gCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCa                                                                                                                         >  1:1256500/1‑150 (MQ=255)
                                    gcgcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCa                                                                                                                   >  1:764489/1‑150 (MQ=255)
                                     cgcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCAt                                                                                                                  <  2:470773/150‑1 (MQ=255)
                                      gcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGgcgct                                                                                                                                                    >  2:1011674/1‑115 (MQ=255)
                                      gcTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGgcgct                                                                                                                                                    <  1:1011674/116‑2 (MQ=255)
                                       cTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGc                                                                                                                <  1:1085807/150‑1 (MQ=255)
                                        tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGcc                                                                                                               >  1:249653/1‑150 (MQ=255)
                                        tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCAc                                                                                                                                                  >  1:1646113/1‑116 (MQ=255)
                                        tGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCAc                                                                                                                                                  <  2:1646113/116‑1 (MQ=255)
                                              aGGCACCGACTCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAcc                                                                                                         >  2:228453/1‑150 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGttt                                                                                                                                           <  2:1594412/113‑1 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACtt                                                                                                                                       <  1:1598833/117‑1 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACtt                                                                                                                                       >  2:1598833/1‑117 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAc                                                                                                                                    <  2:888526/120‑1 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAc                                                                                                                                    >  1:888526/1‑120 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCtat                                                                                                     >  1:499300/1‑150 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCa                                                                                                                                                   <  1:1878835/105‑1 (MQ=255)
                                                  aCCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGCCGTGCAGGCCGCCa                                                                                                                                                   >  2:1878835/1‑105 (MQ=255)
                                                        ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAATCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTc                                                                                               >  1:268489/1‑150 (MQ=255)
                                                        ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAc                                                                                                          <  2:1052020/139‑1 (MQ=255)
                                                        ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAc                                                                                                          >  1:1052020/1‑139 (MQ=255)
                                                        ccGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTc                                                                                               >  2:1129012/1‑150 (MQ=255)
                                                         cGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCa                                                                                              <  2:89763/150‑1 (MQ=255)
                                                         cGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAACCTGGCCAGT‑CATGCCGCAACCCTATATTTTCa                                                                                              <  1:1871759/150‑1 (MQ=255)
                                                              gggCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCCGGCACCTACGTTCACGAGCGTTGAGTCACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGAcc                                                                                         <  1:2309161/150‑1 (MQ=255)
                                                                gCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGt                                                                                       >  1:1785729/1‑150 (MQ=255)
                                                                   aCCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGa                                                                                    <  1:1735753/150‑1 (MQ=255)
                                                                      gTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCt                                                                                 <  1:741771/150‑1 (MQ=255)
                                                                       tACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg                                                                                <  2:764489/150‑1 (MQ=255)
                                                                       tACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg                                                                                <  1:1780869/150‑1 (MQ=255)
                                                                       tACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTg                                                                                <  2:499300/150‑1 (MQ=255)
                                                                       tACCTATGCCGCCGGCGAAGCCTCCCCAGGCACATACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGTAACCCTATATTTTCAGGACCGTCGAGCTg                                                                                <  2:1215171/150‑1 (MQ=255)
                                                                         ccTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGa                                                                              <  1:2175314/150‑1 (MQ=255)
                                                                           tATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGATCACGAGCGTTGAGTTACAGCGCCGTGGCAGCCTGCACGGCGCCACGGTGTTTACTTCCCCCAAG‑AGTCACGTGCATCCCGCAACCCTATATTCTCAGGACCGTCGAGCTGGACa                                                                            >  1:497434/1‑150 (MQ=255)
                                                                            aTGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCAt                                                                                                                  <  2:1220893/111‑1 (MQ=255)
                                                                            aTGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCAt                                                                                                                  >  1:1220893/1‑111 (MQ=255)
                                                                               ccgccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCa                                                                        <  1:2322316/150‑1 (MQ=255)
                                                                               ccgccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCa                                                                        <  2:464920/150‑1 (MQ=255)
                                                                                cgccgccgcAGCCTACCCCGGCACCTACGTTCACCAGCGTTGAGCTACAGCGAAGCGGGGTCCTGCACGGCGCCACGGTGTTTACTTTACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAg                                                                       <  2:572053/150‑1 (MQ=255)
                                                                                  ccgGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCaa                                                                                                                                <  1:2179058/92‑1 (MQ=255)
                                                                                      cGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATc                                                                 <  1:2355462/150‑1 (MQ=255)
                                                                                       gAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATcc                                                                >  2:1997372/1‑150 (MQ=255)
                                                                                       gAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATcc                                                                >  1:1788742/1‑150 (MQ=255)
                                                                                        aaGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCg                                                               >  1:1856468/1‑150 (MQ=255)
                                                                                            cTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCAc                                                                                                                        >  2:69948/1‑89 (MQ=255)
                                                                                            cTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCAc                                                                                                                        <  1:69948/89‑1 (MQ=255)
                                                                                              ccccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGGCAACCAGTCCATCCGCACCGc                                                         <  1:2075689/150‑1 (MQ=255)
                                                                                              ccccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGc                                                         >  1:2016525/1‑150 (MQ=255)
                                                                                               cccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCt                                                        <  2:1856468/150‑1 (MQ=255)
                                                                                                ccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGt                                                                      <  2:1585180/135‑1 (MQ=255)
                                                                                                ccAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGt                                                                      >  1:1585180/1‑135 (MQ=255)
                                                                                                   ggCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTc                                                    <  2:1648082/150‑1 (MQ=255)
                                                                                                   ggCACCTACGTTCACGACCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTc                                                    <  1:1002319/150‑1 (MQ=255)
                                                                                                      aCCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCg                                                                                        >  1:974078/1‑111 (MQ=255)
                                                                                                        cTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTg                                                       <  2:554845/142‑1 (MQ=255)
                                                                                                         tACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGa                                                                                           >  2:249345/1‑105 (MQ=255)
                                                                                                         tACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCATCGGTGTTCGTCCCg                                              <  2:1793431/150‑1 (MQ=255)
                                                                                                            gTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCa                                           >  2:533549/1‑150 (MQ=255)
                                                                                                             ttCACGAGCGTTGAGTTACAGCGCTGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCaa                                          <  1:120806/150‑1 (MQ=255)
                                                                                                                aCGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGcc                                       >  1:1287069/1‑150 (MQ=255)
                                                                                                                  gAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGc                                     >  1:625636/1‑150 (MQ=255)
                                                                                                                  gAGCGTTGAGTTACAACGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCAACCAAC‑AGTCACGTGCACCACGCAACCCCATACTTTCAGGACAGTCGCGCTGGCAAACCAGTCCATCCGCACCGCTGTTCGCACCGGCAAGCCGc                                     >  2:109731/1‑150 (MQ=255)
                                                                                                                    gCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCAc                                   <  1:1606725/150‑1 (MQ=255)
                                                                                                                      gTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCt                                 <  1:1129012/150‑1 (MQ=255)
                                                                                                                          aGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAcc                                                                                                         <  2:1918638/74‑1 (MQ=255)
                                                                                                                          aGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAAcc                                                                                                         >  1:1918638/1‑74 (MQ=255)
                                                                                                                            ttACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATc                                                                 >  2:1196187/1‑112 (MQ=255)
                                                                                                                            ttACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGcc                           >  1:2007132/1‑150 (MQ=255)
                                                                                                                                 gcgcCGTGGCGGCCTGCGCGGCGCCACGGTGTTGACGTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCGCCTGACGCCGTTGc                      <  2:497434/150‑1 (MQ=255)
                                                                                                                                   gcCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTg                    >  1:2169539/1‑150 (MQ=255)
                                                                                                                                   accGTGGCGCCGTGCAGGCCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTg                    >  1:2181763/2‑150 (MQ=255)
                                                                                                                                      gTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATc                 >  1:1620188/1‑150 (MQ=255)
                                                                                                                                       tGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCCCTGGCCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAAACGCAACTCACGCCGTTGCTGATCt                >  1:1955862/1‑150 (MQ=255)
                                                                                                                                         gcggcCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCttt              >  1:2003585/1‑150 (MQ=255)
                                                                                                                                           ggcCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTaa            >  2:2220714/1‑150 (MQ=255)
                                                                                                                                           ggcCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTaa            <  2:191750/150‑1 (MQ=255)
                                                                                                                                               tGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCGACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAGGCCGCAc                                   <  2:96518/123‑1 (MQ=255)
                                                                                                                                               tGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCGACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAGGCCGCAc                                   >  1:96518/1‑123 (MQ=255)
                                                                                                                                                 cACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCAt      <  2:2003585/150‑1 (MQ=255)
                                                                                                                                                     gcgcCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCATCGgc  >  1:1661921/1‑150 (MQ=255)
                                                                                                                                                     gcgcCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCATCGgc  <  1:1806360/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTGCACTGGCAGAGTTGGCTGGGCGAGCGTGCCGGCGCGCTGAAAAAGGCACCGACCCGCCTGGGCAACCGTACCTATGCCGCCGGCGAAGCCTCCCCAGGCACCTACGTTCACGAGCGTTGAGTTACAGCGCCGTGGCGGCCTGCACGGCGCCACGGTGTTTACTTCACCCAAG‑AGTCACGTGCATGCCGCAACCCTATATTTTCAGGACCGTCGAGCTGGACAACCAGTCCATCCGCACCGCTGTTCGCCCCGGCAAGCCGCACCTGACGCCGTTGCTGATCTTTAACGGCATCGGC  >  NC_002947/5701136‑5701434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A