Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F60 I0 R1	1078	79.7	3885236	94.2%	3659892	134.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,655,687	C→A	21.6%	intergenic (‑91/+61)	epd ← / ← tktA	D‑erythrose 4‑phosphate dehydrogenase/transketolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,655,687	0	C	A	21.6%	225.6 / 49.8	102	intergenic (‑91/+61)	epd/tktA	D‑erythrose 4‑phosphate dehydrogenase/transketolase A
Reads supporting (aligned to +/- strand): ref base C (41/39); new base A (11/11); total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 102 total aligned reads displayed.

ACAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCG  >  NC_002947/5655540‑5655833

acaGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCaa                                                                                                                                                   >  2:84783/1‑150 (MQ=255)

 caGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAAc                                                                                                                                                  <  2:1099025/150‑1 (MQ=255)

 caGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAAc                                                                                                                                                  <  2:517383/150‑1 (MQ=255)

   gCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACct                                                                                                                                                >  1:858878/1‑150 (MQ=255)

      gCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccctg                                                                                                                                              <  1:1739877/150‑4 (MQ=38)

       ccGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTGGGGGCTCACGAGACCGTTCAAGTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt                                                                                                                                            <  2:942748/150‑1 (MQ=255)

       ccGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccctgt                                                                                                                                             <  1:374457/150‑5 (MQ=38)

       ccGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCtgt                                                                                                                                            <  2:858878/150‑1 (MQ=255)

         taTACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg                                                                                                                                          <  1:1250406/149‑1 (MQ=255)

         gATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTgg                                                                                                                                          <  1:871031/150‑1 (MQ=255)

            aCGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTAGTGAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAg                                                                                                                                       >  1:1099041/1‑150 (MQ=255)

             cGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGc                                                                                                                                      <  1:1521517/150‑1 (MQ=255)

              ggCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccctgt                                                                                                                                             >  2:371305/1‑139 (MQ=38)

              ggCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccctgt                                                                                                                                             <  1:371305/143‑5 (MQ=38)

              ggCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCg                                                                                                                                     <  2:1759763/150‑1 (MQ=255)

                 cATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGTGGGCGAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGc                                                                                                                                  <  2:274241/150‑1 (MQ=255)

                    aaCCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCaa                                                                                                                                                   >  1:648551/1‑130 (MQ=255)

                    aaCCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCaa                                                                                                                                                   <  2:648551/130‑1 (MQ=255)

                         ttGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCcgc                                                                                                                          <  2:1591760/150‑1 (MQ=255)

                              tGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑g                                                                                                                    >  2:1400852/1‑150 (MQ=255)

                                    tttGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCAATCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCg                                                                                                              >  1:1773634/1‑150 (MQ=255)

                                       gTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTgg                                                                                                                                          >  2:72787/1‑120 (MQ=25)

                                       gTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTgg                                                                                                                                          <  1:72787/120‑1 (MQ=25)

                                        tAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GCAGGCGacac                                                                                                          >  2:611937/1‑150 (MQ=255)

                                             aCGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCcgcg                                                                                                                         <  2:638542/131‑1 (MQ=255)

                                             aCGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCcgcg                                                                                                                         >  1:638542/1‑131 (MQ=255)

                                                gTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGAGGGAACCCACAG‑GGTTTCATGCAACCTGTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCa                                                                                                  >  1:1491753/1‑150 (MQ=255)

                                                       aTTCGGGGCTCACGAGACAGTTCGATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCg                                                                                                                                     >  2:844138/1‑109 (MQ=255)

                                                       aTTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCg                                                                                                                                     <  1:844138/109‑1 (MQ=255)

                                                        ttCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAg                                                                                                                                       >  2:225636/1‑106 (MQ=21)

                                                        ttCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAg                                                                                                                                       <  1:225636/106‑1 (MQ=21)

                                                            gggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGCATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGGCCGAAGATCTCTAAAta                                                                                      >  2:700203/1‑149 (MQ=255)

                                                             ggCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCa                                                                                     >  1:1465323/1‑150 (MQ=255)

                                                                tCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccctgt                                                                                                                                             >  2:510235/1‑89 (MQ=21)

                                                                tCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAAccctgt                                                                                                                                             <  1:510235/93‑5 (MQ=21)

                                                                tCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTc                                                                                  >  1:1097220/1‑150 (MQ=255)

                                                                  aCGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATc                                                                                              >  2:1904752/1‑136 (MQ=255)

                                                                  aCGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATc                                                                                              <  1:1904752/136‑1 (MQ=255)

                                                                   cGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCgg                                                                                                                                    >  1:496917/1‑98 (MQ=255)

                                                                   cGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCgg                                                                                                                                    <  2:496917/98‑1 (MQ=255)

                                                                    gagaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTggg                                                                                                                                         >  2:745886/1‑92 (MQ=18)

                                                                    gagaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTggg                                                                                                                                         <  1:745886/92‑1 (MQ=18)

                                                                     agaCAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCca                                                                             <  1:955546/150‑1 (MQ=255)

                                                                         aGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCcagcag                                                                         >  2:625454/1‑150 (MQ=255)

                                                                         aGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGACCGCAGATCTTTAAATCAGTCTTCcagcag                                                                         >  2:686627/1‑150 (MQ=255)

                                                                           ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGa                                                                                                                                        >  2:561823/1‑86 (MQ=17)

                                                                           ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGa                                                                                                                                        <  1:561823/86‑1 (MQ=17)

                                                                           ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCttt                                                                                           <  2:953196/130‑1 (MQ=255)

                                                                           ttCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGct                                                                       >  1:1898291/1‑150 (MQ=255)

                                                                              aaTTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCtt                                                                    >  1:1895367/1‑150 (MQ=255)

                                                                               aTTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTc                                                                   >  1:501788/1‑150 (MQ=255)

                                                                                ttGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCg                                                                  <  2:1305005/150‑1 (MQ=255)

                                                                                   tcgtcAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGCGCTAGCGGGCTTGCCAGCGAATGGGCGGCG‑GAAGGCCTGGATATCGTTAAATCAGTCTTCCAGCCGCTCTTCGGCa                                                               >  1:1886208/1‑150 (MQ=25)

                                                                                    cgtcAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAg                                                              <  2:1491753/150‑1 (MQ=255)

                                                                                      tcAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGCCTTCCAGCAGCTCTTCGGCAGTa                                                            >  1:111995/1‑150 (MQ=255)

                                                                                         gCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTAGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCg                                                         <  1:386730/150‑1 (MQ=255)

                                                                                            tGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGGAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAgg                                                      >  2:1594552/1‑150 (MQ=255)

                                                                                             gCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGa                                                     <  2:1898291/150‑1 (MQ=255)

                                                                                             gCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGa                                                     <  2:1605696/150‑1 (MQ=255)

                                                                                                        ggTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAgg                                          <  2:1726341/150‑1 (MQ=255)

                                                                                                        ggTGAGGCATTCTCGGGCAAGCCCGCTCCCACAG‑GGTTTTATGCAAGCGCTGTGGGAGCGGGCTAGCCCGCGAAT‑AAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCGAGCAGCTCTTCGGCAATACCGAGAATGTTCTCCAgg                                          >  2:483126/1‑150 (MQ=38)

                                                                                                             ggCATTCGCGGGCAAGCCCGCTGCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGaa                                     <  1:700203/150‑1 (MQ=255)

                                                                                                               gatgcgcgGGCAAGCCCGCTCCCCTAG‑GGTTTCATACAACATTTTTGTGAGCTGGCTTGCGCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGc                                   <  2:1773634/146‑1 (MQ=255)

                                                                                                                 ttCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTGAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCa                                 >  1:732926/1‑150 (MQ=255)

                                                                                                                 ttCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                       <  2:1160508/128‑1 (MQ=255)

                                                                                                                 ttCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                       >  1:1160508/1‑128 (MQ=255)

                                                                                                                    gcgGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCtt                                                                                                                                <  2:1586570/53‑1 (MQ=255)

                                                                                                                    gcgGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCtt                                                                                                                                >  1:1586570/1‑53 (MQ=255)

                                                                                                                    gcgGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                       <  2:1073474/125‑1 (MQ=255)

                                                                                                                    gcgGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                       >  1:1073474/1‑125 (MQ=255)

                                                                                                                       tgCAAGCCCCCTCCCACAT‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAActct                           <  2:501788/149‑1 (MQ=255)

                                                                                                                         cAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTc                         <  1:84783/150‑1 (MQ=255)

                                                                                                                            gCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCa                                                                                     <  1:329725/87‑1 (MQ=255)

                                                                                                                            gCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCa                                                                                     >  2:329725/1‑87 (MQ=255)

                                                                                                                            gCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGaa                      >  2:1824772/1‑150 (MQ=255)

                                                                                                                             cccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAActct                           >  2:833148/1‑144 (MQ=37)

                                                                                                                             cccGCTCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAActct                           <  1:833148/144‑1 (MQ=37)

                                                                                                                              ccGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTa                                                            <  2:169719/110‑1 (MQ=255)

                                                                                                                              ccGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTa                                                            >  1:169719/1‑110 (MQ=255)

                                                                                                                                gaccccACAG‑CGTTTCTTTCGATCTCGGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACAGACCGCGGGTCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAAt                  <  1:611937/147‑1 (MQ=255)

                                                                                                                                gCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAAt                  >  1:1645337/1‑150 (MQ=255)

                                                                                                                                gCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAAt                  <  2:1097220/150‑1 (MQ=255)

                                                                                                                                gCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAAt                  >  1:1099604/1‑150 (MQ=255)

                                                                                                                                gCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAAt                  <  1:1870296/150‑1 (MQ=255)

                                                                                                                                  tCCCACAGAGGTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGc                >  1:1309673/1‑150 (MQ=38)

                                                                                                                                    ccACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGaa                                     <  1:252190/127‑1 (MQ=255)

                                                                                                                                    ccACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGaa                                     >  2:252190/1‑127 (MQ=255)

                                                                                                                                     cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGa             >  2:482027/7‑150 (MQ=38)

                                                                                                                                     cacagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGa             >  1:965269/7‑150 (MQ=38)

                                                                                                                                      acagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGAt                                                    >  1:671660/6‑110 (MQ=25)

                                                                                                                                      acagaggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGAt                                                    <  2:671660/105‑1 (MQ=25)

                                                                                                                                      acaG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGaa            >  1:113197/1‑150 (MQ=255)

                                                                                                                                         g‑ggTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                       <  1:1707267/104‑1 (MQ=255)

                                                                                                                                         g‑ggTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAg                                                       >  2:1707267/1‑104 (MQ=255)

                                                                                                                                           ggTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGAt                                                    <  1:1439409/106‑1 (MQ=255)

                                                                                                                                           ggTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGAt                                                    >  2:1439409/1‑106 (MQ=255)

                                                                                                                                           ggTTGCATGAAACC‑CTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCgg       <  1:482027/150‑1 (MQ=255)

                                                                                                                                                cATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGACCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGcc   <  1:686627/150‑1 (MQ=255)

                                                                                                                                                 aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑TAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTATTCGGCCGTACAGAGGCTGTTCTCCATGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCg  >  2:1876490/1‑150 (MQ=255)

                                                                                                                                                 aTGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCg  >  1:1679674/1‑150 (MQ=255)

ACAGCGGCCGATACGGCCATAACCGTTGAGTGCAACTTTGTAGGGACGGTGGGGCATTCGGGGCTCACGAGACAGTTCAATTGTCGTCAGCCTGCGGAATTTGCGGTGTGGCATTCGCGGGCAAGCCCGCTCCCACAG‑GGTTTCATGCAACCTCTGTGGGAGCGGGCTTGCCCGCGAAT‑GAAGGCGACACCGCCCGCAGATCTTTAAATCAGTCTTCCAGCAGCTCTTCGGCAGTACCGAGGATGTTCTCCAGGGTGAAGCCAAACTCTTCGAACAATGCCGAAGCCGGCGCCG  >  NC_002947/5655540‑5655833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A