Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,744,958 G→A G82E (GGA→GAA)  lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,744,9580GA99.6% 1819.3 / NA 500G82E (GGA→GAA) lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (255/242);  minor base C (2/0);  total (258/242)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 521 total aligned reads displayed.

GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC  >  NC_000913/3744868‑3745047
                                                                                          |                                                                                         
ggCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaa                                                                                         <  1:4121851/93‑1 (MQ=255)
 gCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaaa                                                                                        >  2:729875/1‑93 (MQ=255)
 gCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaaa                                                                                        <  2:5220775/93‑1 (MQ=255)
   aaTGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAgg                                                                                      >  1:530102/1‑93 (MQ=255)
      gctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTGTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCt                                                                                   <  2:10046809/93‑1 (MQ=255)
      gctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCt                                                                                   <  1:6145448/93‑1 (MQ=255)
      gcggcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCt                                                                                   <  2:657426/90‑1 (MQ=255)
       ctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCtt                                                                                  <  2:5895725/93‑1 (MQ=255)
         gcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGt                                                                                <  2:9801599/93‑1 (MQ=255)
          cATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtt                                                                               <  2:3229973/93‑1 (MQ=255)
           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              >  2:9044030/1‑93 (MQ=255)
           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              >  1:6602531/1‑93 (MQ=255)
           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              <  2:563599/93‑1 (MQ=255)
            tGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtttt                                                                             <  1:7689064/93‑1 (MQ=255)
            tGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtttt                                                                             >  1:1365248/1‑93 (MQ=255)
            tGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtttt                                                                             >  2:4094733/1‑93 (MQ=255)
             ggCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttttt                                                                            <  1:6384138/93‑1 (MQ=255)
             ggCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttttt                                                                            <  1:2040872/93‑1 (MQ=255)
              gCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTg                                                                           >  1:1831736/1‑93 (MQ=255)
              gCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTg                                                                           >  2:6145672/1‑93 (MQ=255)
               cTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGc                                                                          >  2:2810500/1‑93 (MQ=255)
                tGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCt                                                                         >  1:8527631/1‑93 (MQ=255)
                 gTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTg                                                                        <  2:7865886/93‑1 (MQ=255)
                  tCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTgg                                                                       >  1:3038009/1‑93 (MQ=255)
                   cATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGa                                                                      <  1:8361795/93‑1 (MQ=255)
                   cATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGa                                                                      <  2:6927585/93‑1 (MQ=255)
                    aTTCGCGCCCTGCTTACTCATTCTGGTGTTTGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAt                                                                     >  2:3547501/1‑93 (MQ=255)
                    aTTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAt                                                                     <  1:1917387/93‑1 (MQ=255)
                     ttCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATa                                                                    >  2:4785983/1‑93 (MQ=255)
                      tCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATaa                                                                   <  2:1039856/93‑1 (MQ=255)
                         cgcCCTGCTTGCTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATaaaaa                                                                <  2:7970358/93‑1 (MQ=255)
                          gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               >  2:4298168/1‑93 (MQ=255)
                           cccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACg                                                              >  1:4611417/1‑93 (MQ=255)
                            ccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGACAAGGGCTTGTTTTTGCTGGATAAAAACGa                                                             >  1:10003839/1‑93 (MQ=255)
                             cTGCTTTCTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAc                                                            <  2:626376/93‑1 (MQ=255)
                             cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAc                                                            >  2:3695990/1‑93 (MQ=255)
                             cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAc                                                            >  2:1283542/1‑93 (MQ=255)
                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           <  2:2520408/93‑1 (MQ=255)
                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           <  2:1383609/93‑1 (MQ=255)
                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           >  2:7037385/1‑93 (MQ=255)
                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           <  2:1528971/93‑1 (MQ=255)
                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACa                                                           >  1:704590/1‑93 (MQ=255)
                               gCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACaa                                                          >  2:8897098/1‑93 (MQ=255)
                                cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGTTGTATAAAAATGACaac                                                         >  1:5071442/1‑92 (MQ=255)
                                cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACaaa                                                         <  2:6059690/93‑1 (MQ=255)
                                cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACaaa                                                         <  1:7440141/93‑1 (MQ=255)
                                 ttACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAc                                                        >  1:7894126/1‑93 (MQ=255)
                                  tACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAcc                                                       >  1:5168199/1‑93 (MQ=255)
                                  tACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAcc                                                       >  2:4465321/1‑93 (MQ=255)
                                   aCTCATTCTGGTGTTAGCGGGGTACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCg                                                      <  1:9166916/93‑1 (MQ=255)
                                      taTTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   <  1:3174097/92‑1 (MQ=255)
                                      cATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   <  1:871978/93‑1 (MQ=255)
                                      cATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   <  1:10046056/93‑1 (MQ=255)
                                        ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCgg                                                 >  1:8838869/1‑93 (MQ=255)
                                         tCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAACGCTCggg                                                >  1:4030979/1‑93 (MQ=255)
                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              <  1:8146953/93‑1 (MQ=255)
                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              >  2:8464533/1‑93 (MQ=255)
                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              <  2:1881254/93‑1 (MQ=255)
                                            ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAt                                             >  1:2763260/1‑93 (MQ=255)
                                              tgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGc                                           >  2:7515038/1‑93 (MQ=255)
                                              tgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGc                                           <  2:5489143/93‑1 (MQ=255)
                                               gtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCt                                          >  2:5018510/1‑93 (MQ=255)
                                                ttAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTa                                         >  2:9296981/1‑93 (MQ=255)
                                                ttAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTa                                         >  1:1480301/1‑93 (MQ=255)
                                                  aGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAtt                                       <  1:5732207/93‑1 (MQ=255)
                                                   gCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAttt                                      >  1:5446247/1‑93 (MQ=255)
                                                   gCGGGGAACAAATTGTAGGTATCGGCATCTCCGCACAGGAACAGGGCTTGTTTTTGCTGGATAAAAACGACAATCCGCTCGGGAATGCTAttt                                      <  1:8985288/93‑1 (MQ=255)
                                                     ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAttttt                                    >  2:8553367/1‑92 (MQ=255)
                                                     ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTg                                    <  2:6818072/93‑1 (MQ=255)
                                                      gggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGt                                   >  2:8104125/1‑93 (MQ=255)
                                                       ggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTc                                  <  2:2337635/93‑1 (MQ=255)
                                                         aaCAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCt                                <  1:9110004/93‑1 (MQ=255)
                                                           cAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCg                              <  1:7332242/93‑1 (MQ=255)
                                                            aaaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCgg                             >  1:358419/1‑93 (MQ=255)
                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                            <  2:161094/93‑1 (MQ=255)
                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                            >  2:3928581/1‑93 (MQ=255)
                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG‑TTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAc                           >  1:3809677/1‑93 (MQ=255)
                                                               ttGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAcc                          <  1:9758641/93‑1 (MQ=255)
                                                                 gTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                        <  1:1692278/93‑1 (MQ=255)
                                                                 gTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                        <  2:2097544/93‑1 (MQ=255)
                                                                 gTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                        <  1:9557219/93‑1 (MQ=255)
                                                                      tATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGc                   >  1:2221552/1‑93 (MQ=255)
                                                                      tATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGc                   <  2:5326122/93‑1 (MQ=255)
                                                                      tATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGc                   >  1:5938009/1‑93 (MQ=255)
                                                                       aTCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCg                  >  2:9540316/1‑93 (MQ=255)
                                                                        tCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGa                 >  1:6437953/1‑93 (MQ=255)
                                                                         cGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGAt                <  1:4951110/93‑1 (MQ=255)
                                                                          ggCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATg               <  2:7129422/93‑1 (MQ=255)
                                                                           gCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATgg              >  1:4221022/1‑93 (MQ=255)
                                                                             aTCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaa            >  1:8450427/1‑93 (MQ=255)
                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACGAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGGTGGaaa           >  2:8248224/1‑93 (MQ=255)
                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaca           >  1:4347674/1‑91 (MQ=255)
                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           >  1:5841986/1‑93 (MQ=255)
                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           >  1:6705944/1‑93 (MQ=255)
                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           >  2:618703/1‑93 (MQ=255)
                                                                               ctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAt          <  1:7545057/93‑1 (MQ=255)
                                                                               ctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAt          >  2:3175233/1‑93 (MQ=255)
                                                                                 ccGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcg        >  2:8162283/1‑93 (MQ=255)
                                                                                   gCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgtt      <  1:2529776/93‑1 (MQ=255)
                                                                                      caGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttcgt   <  2:4597513/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC  >  NC_000913/3744868‑3745047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 281 total aligned reads displayed.
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  >  NC_000913/3744866‑3745051
                                                                                            |                                                                                             
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAA                                                                                              >  SRR4302540.9958248/1‑94 (MQ=60)
 TGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAA                                                                                             <  SRR4302540.5984969/94‑1 (MQ=60)
 TGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAA                                                                                             >  SRR4302540.3170096/1‑94 (MQ=60)
  GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAA                                                                                            <  SRR4302540.4166362/94‑1 (MQ=60)
  GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAA                                                                                            >  SRR4302540.4383989/1‑94 (MQ=60)
   GCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAG                                                                                           <  SRR4302540.8919054/94‑1 (MQ=60)
   GCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAG                                                                                           <  SRR4302540.6778180/94‑1 (MQ=60)
    CAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGG                                                                                          >  SRR4302540.7476873/1‑94 (MQ=60)
    CAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGG                                                                                          >  SRR4302540.535829/1‑94 (MQ=60)
     AATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGG                                                                                         <  SRR4302540.8240802/94‑1 (MQ=60)
     AATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGG                                                                                         >  SRR4302540.6044736/1‑94 (MQ=60)
      ATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGC                                                                                        <  SRR4302540.4084722/94‑1 (MQ=60)
      ATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGC                                                                                        >  SRR4302540.1740106/1‑94 (MQ=60)
       TGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCT                                                                                       <  SRR4302540.1425835/94‑1 (MQ=60)
        GCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGTGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTT                                                                                      >  SRR4302540.9094775/1‑94 (MQ=60)
        GCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTT                                                                                      <  SRR4302540.2183386/94‑1 (MQ=60)
        GCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTT                                                                                      <  SRR4302540.6213899/94‑1 (MQ=60)
         CTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG                                                                                     >  SRR4302540.7535106/1‑94 (MQ=60)
         CTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG                                                                                     >  SRR4302540.4206442/1‑94 (MQ=60)
         CTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG                                                                                     >  SRR4302540.6746547/1‑94 (MQ=60)
         CTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG                                                                                     >  SRR4302540.5341012/1‑94 (MQ=60)
           GCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATATCCGCACAGGAAAACGGCTTGCT                                                                                   >  SRR4302540.9195912/1‑94 (MQ=60)
            CATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTT                                                                                  >  SRR4302540.6675647/1‑94 (MQ=60)
            CATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTT                                                                                  <  SRR4302540.421847/94‑1 (MQ=60)
             ATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTT                                                                                 >  SRR4302540.8616991/1‑94 (MQ=60)
             ATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTT                                                                                 >  SRR4302540.2039621/1‑94 (MQ=60)
             ATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTT                                                                                 >  SRR4302540.2620954/1‑94 (MQ=60)
             ATGGCTGTCATTCGCACCCTGCTTACTCATTCTGGTGTTAGCGGGAAACAAATTGTCGGTATCAGCATCTCCGCACAGGAAAAGGGCTTGTTTT                                                                                 <  SRR4302540.9039783/94‑1 (MQ=60)
              TGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTT                                                                                <  SRR4302540.179329/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               >  SRR4302540.1851777/1‑94 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302540.6455208/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302540.2062861/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302540.6455207/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302540.324704/94‑1 (MQ=60)
                 CTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCT                                                                             >  SRR4302540.8626716/1‑94 (MQ=60)
                  TGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTG                                                                            >  SRR4302540.319576/1‑94 (MQ=60)
                   GTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGG                                                                           >  SRR4302540.6499419/1‑94 (MQ=60)
                   GTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGG                                                                           >  SRR4302540.10158322/1‑94 (MQ=60)
                   GTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGG                                                                           >  SRR4302540.3071354/1‑94 (MQ=60)
                   GTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGG                                                                           >  SRR4302540.1930536/1‑94 (MQ=60)
                    TCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGA                                                                          <  SRR4302540.7186576/94‑1 (MQ=60)
                     CATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAT                                                                         >  SRR4302540.5039544/1‑94 (MQ=60)
                     CATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAT                                                                         <  SRR4302540.8458831/94‑1 (MQ=60)
                      ATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATA                                                                        >  SRR4302540.3665313/1‑94 (MQ=60)
                      ATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATA                                                                        >  SRR4302540.5997596/1‑94 (MQ=60)
                      ATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATA                                                                        >  SRR4302540.8720640/1‑94 (MQ=60)
                      ATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATA                                                                        >  SRR4302540.2008494/1‑94 (MQ=60)
                       TTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAA                                                                       <  SRR4302540.4564538/94‑1 (MQ=60)
                        TCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAA                                                                      <  SRR4302540.6899558/94‑1 (MQ=60)
                         CGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAA                                                                     <  SRR4302540.2305330/94‑1 (MQ=60)
                          GCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAA                                                                    <  SRR4302540.7486862/94‑1 (MQ=60)
                          GCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAA                                                                    >  SRR4302540.7857236/1‑94 (MQ=60)
                           CGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAC                                                                   <  SRR4302540.7621743/94‑1 (MQ=60)
                           CGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAC                                                                   >  SRR4302540.2931431/1‑94 (MQ=60)
                           CGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAC                                                                   <  SRR4302540.7677615/94‑1 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAcaaca                                                                  >  SRR4302540.8844889/1‑89 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  >  SRR4302540.4660909/1‑94 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  >  SRR4302540.3703148/1‑94 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  >  SRR4302540.5895004/1‑94 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  >  SRR4302540.5895003/1‑94 (MQ=60)
                             CCCTGCTTACTCATTCTGGTGTTAGCGGGGAACACATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGA                                                                 <  SRR4302540.6282725/94‑1 (MQ=60)
                             CCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGACAAGGGCTTGTTTTTGCTGGATAAAAACGA                                                                 >  SRR4302540.10120363/1‑94 (MQ=60)
                              CCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAC                                                                <  SRR4302540.1900132/94‑1 (MQ=60)
                              CCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAC                                                                >  SRR4302540.895520/1‑94 (MQ=60)
                               CTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACA                                                               >  SRR4302540.2058744/1‑94 (MQ=60)
                               CTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACA                                                               >  SRR4302540.9707846/1‑94 (MQ=60)
                               CTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACA                                                               >  SRR4302540.7746204/1‑94 (MQ=60)
                                TGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAA                                                              <  SRR4302540.5947391/94‑1 (MQ=60)
                                TGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAA                                                              >  SRR4302540.3801756/1‑94 (MQ=60)
                                TGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAA                                                              >  SRR4302540.8518272/1‑94 (MQ=60)
                                 GCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGTTGTATAAAAATGACAAC                                                             >  SRR4302540.5126270/1‑94 (MQ=60)
                                 GCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAA                                                             >  SRR4302540.5370387/1‑94 (MQ=60)
                                 GCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAA                                                             >  SRR4302540.5126271/1‑94 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            <  SRR4302540.6653245/94‑1 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            <  SRR4302540.7523042/94‑1 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            <  SRR4302540.7264779/94‑1 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            >  SRR4302540.7986240/1‑94 (MQ=60)
                                   TTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACC                                                           <  SRR4302540.9756342/94‑1 (MQ=60)
                                    TACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCG                                                          >  SRR4302540.8912466/1‑94 (MQ=60)
                                     ACTCATTCTGGTGTTAGCGGGGTACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGC                                                         <  SRR4302540.9276902/94‑1 (MQ=60)
                                      CTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCT                                                        >  SRR4302540.7746692/1‑94 (MQ=60)
                                      CTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCT                                                        <  SRR4302540.838384/94‑1 (MQ=60)
                                       TCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTC                                                       >  SRR4302540.8945222/1‑94 (MQ=60)
                                       TCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTC                                                       <  SRR4302540.166017/94‑1 (MQ=60)
                                        TATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCG                                                      <  SRR4302540.3208602/94‑1 (MQ=60)
                                        CATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCG                                                      <  SRR4302540.881231/94‑1 (MQ=60)
                                        CATGCTGGTGTTAGCGGGGAACAAATTCTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCG                                                      <  SRR4302540.10162840/94‑1 (MQ=60)
                                          TTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGG                                                    <  SRR4302540.3912237/94‑1 (MQ=60)
                                          TTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAACGCTCGGG                                                    >  SRR4302540.4074445/1‑94 (MQ=60)
                                          TTCTGGTGTTAGCGAGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGG                                                    <  SRR4302540.6520407/94‑1 (MQ=60)
                                           TCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGA                                                   >  SRR4302540.1429316/1‑94 (MQ=60)
                                           TCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGA                                                   <  SRR4302540.5386884/94‑1 (MQ=60)
                                            CTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAA                                                  >  SRR4302540.85509/1‑94 (MQ=60)
                                            CTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAA                                                  >  SRR4302540.796994/1‑94 (MQ=60)
                                            CTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAA                                                  >  SRR4302540.6138232/1‑94 (MQ=60)
                                             TGGTGTTAGCGGGGAAGAAATTGTCGTTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGCCAAACCGCTCGGGAAT                                                 <  SRR4302540.1306973/94‑1 (MQ=60)
                                             TGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAT                                                 <  SRR4302540.9507524/94‑1 (MQ=60)
                                             TGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAT                                                 >  SRR4302540.2793108/1‑94 (MQ=60)
                                             TGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAT                                                 <  SRR4302540.8611513/94‑1 (MQ=60)
                                             TGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAT                                                 <  SRR4302540.8241529/94‑1 (MQ=60)
                                             TGGTGTGAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAT                                                 <  SRR4302540.924316/94‑1 (MQ=60)
                                              GGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATG                                                <  SRR4302540.2286803/94‑1 (MQ=60)
                                              GGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATG                                                <  SRR4302540.3921704/94‑1 (MQ=60)
                                               GTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGC                                               >  SRR4302540.5577544/1‑94 (MQ=60)
                                                TGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCT                                              >  SRR4302540.4082565/1‑94 (MQ=60)
                                                 GTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTA                                             >  SRR4302540.1496517/1‑94 (MQ=60)
                                                 GTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTA                                             >  SRR4302540.420008/1‑94 (MQ=60)
                                                  TTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAT                                            >  SRR4302540.1875133/1‑94 (MQ=60)
                                                  TTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAT                                            >  SRR4302540.3523138/1‑94 (MQ=60)
                                                   TAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATT                                           >  SRR4302540.6741042/1‑94 (MQ=60)
                                                   TAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATT                                           >  SRR4302540.8017021/1‑94 (MQ=60)
                                                    AGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTT                                          <  SRR4302540.9974272/94‑1 (MQ=60)
                                                    AGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTT                                          <  SRR4302540.5795343/94‑1 (MQ=60)
                                                    AGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTT                                          <  SRR4302540.5772047/94‑1 (MQ=60)
                                                    AGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTT                                          >  SRR4302540.5506179/1‑94 (MQ=60)
                                                     GCGGGGAACAAATTGTAGGTATCGGCATCTCCGCACAGGAACAGGGCTTGTTTTTGCTGGATAAAAACGACAATCCGCTCGGGAATGCTATTTT                                         <  SRR4302540.9092603/94‑1 (MQ=60)
                                                      CGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTG                                        <  SRR4302540.10134667/94‑1 (MQ=60)
                                                      CGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTG                                        <  SRR4302540.3069278/94‑1 (MQ=60)
                                                         GGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCC                                     <  SRR4302540.4436409/94‑1 (MQ=60)
                                                          GAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCT                                    >  SRR4302540.7039180/1‑94 (MQ=60)
                                                           AACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTC                                   <  SRR4302540.9219487/94‑1 (MQ=60)
                                                           AACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTC                                   <  SRR4302540.1376406/94‑1 (MQ=60)
                                                            ACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCG                                  <  SRR4302540.5937507/94‑1 (MQ=60)
                                                             CAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGG                                 >  SRR4302540.362106/1‑94 (MQ=60)
                                                             CAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGG                                 <  SRR4302540.7414091/94‑1 (MQ=60)
                                                              AAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGA                                <  SRR4302540.5125843/94‑1 (MQ=60)
                                                              AAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG‑TTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAC                               >  SRR4302540.3851169/1‑94 (MQ=60)
                                                               AATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAC                               >  SRR4302540.1695903/1‑94 (MQ=60)
                                                                ATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACC                              <  SRR4302540.4771825/94‑1 (MQ=60)
                                                                 TTGTCGTGATCGGCATCTCCGCACAGGAAAAGGTCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCG                             <  SRR4302540.9872933/94‑1 (MQ=60)
                                                                 TTGTCGGTATCGGCATCTCCGCACAGGAAAAGTGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCG                             <  SRR4302540.4320940/94‑1 (MQ=60)
                                                                 TTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCG                             <  SRR4302540.9873111/94‑1 (MQ=60)
                                                                 TTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCG                             >  SRR4302540.6533271/1‑94 (MQ=60)
                                                                   GTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCC                           <  SRR4302540.9670232/94‑1 (MQ=60)
                                                                   GTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCC                           >  SRR4302540.8345902/1‑94 (MQ=60)
                                                                   GTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCC                           <  SRR4302540.1710629/94‑1 (MQ=60)
                                                                     CGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGG                         <  SRR4302540.6170579/94‑1 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAACAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGCATGCTATTTTGTCCTCGGACCCGCGGG                        >  SRR4302540.8488612/1‑94 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGTTATTTTGTCCTCGGACCGCCGGG                        >  SRR4302540.8819591/1‑94 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGG                        >  SRR4302540.9156930/1‑94 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGG                        <  SRR4302540.9517188/94‑1 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGG                        <  SRR4302540.10082875/94‑1 (MQ=60)
                                                                       GTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGC                       >  SRR4302540.2245502/1‑94 (MQ=60)
                                                                       GTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGC                       >  SRR4302540.1807173/1‑94 (MQ=60)
                                                                        TATCGGCATCTCCGCACAGGAAAAGGTCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCG                      <  SRR4302540.925521/94‑1 (MQ=60)
                                                                        TATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCG                      >  SRR4302540.143006/1‑94 (MQ=60)
                                                                        TATCGGCATCTCCGAACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCG                      >  SRR4302540.6790944/1‑94 (MQ=60)
                                                                         ATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGA                     >  SRR4302540.6230950/1‑94 (MQ=60)
                                                                         ATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGA                     >  SRR4302540.6509605/1‑94 (MQ=60)
                                                                          TCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGAT                    <  SRR4302540.5208365/94‑1 (MQ=60)
                                                                           CGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATG                   >  SRR4302540.4152044/1‑94 (MQ=60)
                                                                             GCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGA                 <  SRR4302540.3409761/94‑1 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                <  SRR4302540.7398040/94‑1 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                >  SRR4302540.8548534/1‑94 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                <  SRR4302540.867867/94‑1 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                >  SRR4302540.2092802/1‑94 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                >  SRR4302540.5728134/1‑94 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                >  SRR4302540.4595398/1‑94 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGACA               >  SRR4302540.4394976/1‑94 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAA               >  SRR4302540.4683039/1‑94 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAA               >  SRR4302540.5268573/1‑94 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAA               <  SRR4302540.521377/94‑1 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAA               >  SRR4302540.7028825/1‑94 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAA               >  SRR4302540.5906588/1‑94 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAA               >  SRR4302540.2210257/1‑94 (MQ=60)
                                                                               ATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAA               >  SRR4302540.5055309/1‑94 (MQ=60)
                                                                                TCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAT              >  SRR4302540.3024102/1‑94 (MQ=60)
                                                                                 CTCCGCACAGGAACAGGGCTTGTTTTTGCTGGATAAAAACGACAAAACGCTCGGGAATGCTATTTTGACCTCTGACCGCCGGcggctgaaaatc             >  SRR4302540.6485374/1‑82 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGTCCGCCGGGCGACGGCAATC             >  SRR4302540.5361427/1‑94 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATC             <  SRR4302540.7629613/94‑1 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATC             >  SRR4302540.5234356/1‑94 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATC             <  SRR4302540.7361010/94‑1 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCAATTTTGTCCTCGGACGCCCGGGCGATGGAActg             >  SRR4302540.7974006/1‑91 (MQ=60)
                                                                                  TCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCG            >  SRR4302540.1222003/1‑94 (MQ=60)
                                                                                    CGCGCAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAGCCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTT          <  SRR4302540.4303622/94‑1 (MQ=60)
                                                                                    CGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTT          >  SRR4302540.9301815/1‑94 (MQ=60)
                                                                                    CGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTT          >  SRR4302540.8054666/1‑94 (MQ=60)
                                                                                     GCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTC         <  SRR4302540.1606233/94‑1 (MQ=60)
                                                                                     GCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTC         >  SRR4302540.1362786/1‑94 (MQ=60)
                                                                                     GCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTC         <  SRR4302540.6221027/94‑1 (MQ=60)
                                                                                     GCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTC         <  SRR4302540.2557073/94‑1 (MQ=60)
                                                                                      CACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCT        >  SRR4302540.3956085/1‑94 (MQ=60)
                                                                                       ACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGT       >  SRR4302540.4872887/1‑94 (MQ=60)
                                                                                       ACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGT       >  SRR4302540.4872886/1‑94 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATACAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCCTCG     >  SRR4302540.3924031/1‑94 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCG     >  SRR4302540.6226886/1‑94 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCG     <  SRR4302540.2458152/94‑1 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCG     <  SRR4302540.7549873/94‑1 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCG     >  SRR4302540.7806561/1‑94 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCG     >  SRR4302540.9453291/1‑94 (MQ=60)
                                                                                          GGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAATCCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGC    <  SRR4302540.8203715/94‑1 (MQ=60)
                                                                                          GGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGC    >  SRR4302540.5389157/1‑94 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   <  SRR4302540.3492555/94‑1 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   <  SRR4302540.8519315/94‑1 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   <  SRR4302540.1194352/94‑1 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   >  SRR4302540.3055810/1‑94 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   >  SRR4302540.7046736/1‑94 (MQ=60)
                                                                                            AAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  <  SRR4302540.3863833/94‑1 (MQ=60)
                                                                                            AAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  >  SRR4302540.8481370/1‑94 (MQ=60)
                                                                                            AAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  <  SRR4302540.2571843/94‑1 (MQ=60)
                                                                                            |                                                                                             
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  >  NC_000913/3744866‑3745051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑