Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,744,796	G→A	G28D (GGC→GAC)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,744,796	0	G	A	100.0%	814.8 / NA	229	G28D (GGC→GAC)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (101/128); total (101/128)

Only 100 of 253 total aligned reads displayed.

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTG  >  NC_000913/3744706‑3744885

aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACg                                                                                         <  1:2224279/93‑1 (MQ=255)

  tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTg                                                                                       >  2:2231327/1‑93 (MQ=255)

   aaaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGc                                                                                      <  1:6247818/93‑1 (MQ=255)

   aaaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGc                                                                                      <  1:128695/93‑1 (MQ=255)

        aTGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcgc                                                                                 >  1:546515/1‑93 (MQ=255)

        aTGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcgc                                                                                 >  2:3227903/1‑93 (MQ=255)

        aTGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcgc                                                                                 >  1:3169869/1‑93 (MQ=255)

         tGATGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                >  1:1123843/1‑93 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                >  2:491402/1‑93 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                <  1:5672307/93‑1 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                <  2:1896570/93‑1 (MQ=255)

         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                >  1:1531038/1‑93 (MQ=255)

          gACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCt                                                                               >  2:3797937/1‑93 (MQ=255)

           aCGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTg                                                                              <  2:4211074/93‑1 (MQ=255)

               aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                          >  1:3097227/1‑93 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTTAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                        >  1:4630163/1‑93 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                        >  1:6139782/1‑93 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                        <  1:262459/93‑1 (MQ=255)

                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                        >  1:1588200/1‑93 (MQ=255)

                   ctggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtgtg                                                                      >  2:4701069/1‑93 (MQ=255)

                     ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcg                                                                    <  1:1834268/93‑1 (MQ=255)

                      gctggGGTTAGATTGGGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcgc                                                                   <  2:2589217/93‑1 (MQ=255)

                        tggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCTGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAt                                                                 >  2:2032720/1‑93 (MQ=255)

                        tggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAt                                                                 >  1:3360281/1‑93 (MQ=255)

                         gggtTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                <  2:2272930/93‑1 (MQ=255)

                         ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                >  1:2176085/1‑93 (MQ=255)

                          gggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTa                                                               >  1:2428617/1‑93 (MQ=255)

                           ggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTaa                                                              <  2:597051/93‑1 (MQ=255)

                            gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                             >  2:616411/1‑93 (MQ=255)

                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                           <  2:2564388/93‑1 (MQ=255)

                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                           <  1:4603324/93‑1 (MQ=255)

                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCATACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                          <  1:4346026/93‑1 (MQ=255)

                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                          >  2:106767/1‑93 (MQ=255)

                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                          <  2:2172476/93‑1 (MQ=255)

                                gATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCg                                                         <  2:5549972/93‑1 (MQ=255)

                                gATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCg                                                         <  2:1178913/93‑1 (MQ=255)

                                gATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCg                                                         <  2:3980948/93‑1 (MQ=255)

                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGCCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                        <  1:5315309/93‑1 (MQ=255)

                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                        <  2:3437783/93‑1 (MQ=255)

                                  ttGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                       >  1:1023770/1‑93 (MQ=255)

                                  ttGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                       <  2:2865839/93‑1 (MQ=255)

                                    gtgGCGTTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                     <  2:794429/93‑1 (MQ=255)

                                    gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                     >  2:3554025/1‑93 (MQ=255)

                                    gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                     >  2:4839747/1‑93 (MQ=255)

                                    gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                     <  1:2894660/93‑1 (MQ=255)

                                    gtgGCGGTAGCTGGCTGAAAGCCGGGCTGGATGACCGCCAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGGCCGCAGc                                                     >  1:2583687/1‑93 (MQ=255)

                                    ggggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGc                                                     <  2:3063862/91‑1 (MQ=255)

                                       gCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAg                                                  <  2:5367230/93‑1 (MQ=255)

                                         ggTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGc                                                <  1:939649/93‑1 (MQ=255)

                                           tAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTg                                              >  2:1601119/1‑93 (MQ=255)

                                            aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                             >  1:5978186/1‑93 (MQ=255)

                                            aGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTgg                                             <  2:1995914/93‑1 (MQ=255)

                                             gctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                            <  1:1620691/93‑1 (MQ=255)

                                              ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                           <  1:6241062/93‑1 (MQ=255)

                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCGCGCAGCCAGGCTGCGCa                                          >  2:141690/1‑93 (MQ=255)

                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCa                                          >  2:5104605/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  2:1762479/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  2:6057954/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  1:3744330/1‑93 (MQ=255)

                                                ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCag                                         >  2:4502476/1‑93 (MQ=255)

                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                        <  2:2966331/93‑1 (MQ=255)

                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                       <  1:2049853/93‑1 (MQ=255)

                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                       >  1:1904882/1‑93 (MQ=255)

                                                  ctgAAACCCGGGCTGTATGACCGCGAAGCCCGGGAGGCACACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                       <  2:2379335/93‑1 (MQ=255)

                                                   tgAAAGCCGGGCTGTATGACCGAGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgc                                      <  1:195397/93‑1 (MQ=255)

                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                     >  2:5770741/1‑93 (MQ=255)

                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                     >  1:3547340/1‑93 (MQ=255)

                                                     aaaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcgc                                    <  1:5450775/93‑1 (MQ=255)

                                                     aaaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcgc                                    <  2:2677280/93‑1 (MQ=255)

                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                   >  2:3022819/1‑93 (MQ=255)

                                                        gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                 <  1:5862130/93‑1 (MQ=255)

                                                        gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                 >  1:5176102/1‑93 (MQ=255)

                                                         cggggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                <  2:5323917/91‑1 (MQ=255)

                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                >  1:3971504/1‑93 (MQ=255)

                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                <  2:1654640/93‑1 (MQ=255)

                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                               <  2:1304563/93‑1 (MQ=255)

                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                               >  2:5419596/1‑93 (MQ=255)

                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                               <  2:5414501/93‑1 (MQ=255)

                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                               <  2:1469695/93‑1 (MQ=255)

                                                           gggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                              <  1:3087943/93‑1 (MQ=255)

                                                           gggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATg                              <  1:4722534/93‑1 (MQ=255)

                                                              cTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCATGCTGGGCAGAGCGCGATATGGCa                           <  2:5075462/93‑1 (MQ=255)

                                                                  aTGACCGCGAGGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAc                       <  2:3297773/93‑1 (MQ=255)

                                                                    gACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtg                     <  2:2823519/93‑1 (MQ=255)

                                                                     gccGCGAAGGCCGGGAGGCAGACGTGCAGCTCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                    <  2:2929569/92‑1 (MQ=255)

                                                                     aCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgt                    >  1:5061424/1‑93 (MQ=255)

                                                                      ccGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtgtg                   <  2:6095929/93‑1 (MQ=255)

                                                                        gcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGc                 <  1:4474521/93‑1 (MQ=255)

                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt              <  2:4487797/93‑1 (MQ=255)

                                                                            aGGCCGGGAGGGAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtg             <  1:3807216/93‑1 (MQ=255)

                                                                            aGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCCGAGCGCGATATGGCAGAACTGTGGCAAtg             >  1:2804677/1‑93 (MQ=255)

                                                                            aGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtg             >  1:40374/1‑93 (MQ=255)

                                                                              gcggggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct           <  2:2474169/90‑1 (MQ=255)

                                                                              gCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgct           <  1:1472252/93‑1 (MQ=255)

                                                                                cGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGCGCTATGGCAGAACTGTGGCACtgctgc         >  1:1221723/1‑93 (MQ=255)

                                                                                cGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgctgc         <  1:798880/93‑1 (MQ=255)

                                                                                 gggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCa        <  1:3504309/93‑1 (MQ=255)

                                                                                    aggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg     <  1:4999422/93‑1 (MQ=255)

                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGGTATGGCAGAACTGTGGCAATGCTGCATGGc    >  2:4291317/1‑93 (MQ=255)

                                                                                       cagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTg  >  2:1837926/1‑93 (MQ=255)

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTG  >  NC_000913/3744706‑3744885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  >  NC_000913/3744703‑3744889

TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGA                                                                                               >  SRR4302533.1665341/1‑94 (MQ=60)

TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGA                                                                                               >  SRR4302533.4507943/1‑94 (MQ=60)

TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGA                                                                                               >  SRR4302533.2132553/1‑94 (MQ=60)

 GCAATAAACCATGACGCAATACTGGCTGGGTTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGAC                                                                                              <  SRR4302533.4517042/94‑1 (MQ=60)

 GCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGAC                                                                                              <  SRR4302533.3837525/94‑1 (MQ=60)

  CAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACG                                                                                             >  SRR4302533.2617889/1‑94 (MQ=60)

   AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGT                                                                                            <  SRR4302533.2238015/94‑1 (MQ=60)

    ATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTG                                                                                           <  SRR4302533.5940836/94‑1 (MQ=60)

      AAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCA                                                                                         <  SRR4302533.6285206/94‑1 (MQ=60)

      AAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCA                                                                                         <  SRR4302533.129502/94‑1 (MQ=60)

      AAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCA                                                                                         <  SRR4302533.3812446/94‑1 (MQ=60)

       AACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAG                                                                                        <  SRR4302533.2066172/94‑1 (MQ=60)

          CATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGC                                                                                     >  SRR4302533.3189379/1‑94 (MQ=60)

          CATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGC                                                                                     >  SRR4302533.549762/1‑94 (MQ=60)

           ATGATGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCC                                                                                    >  SRR4302533.1130512/1‑94 (MQ=60)

           ATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCC                                                                                    >  SRR4302533.1540240/1‑94 (MQ=60)

            TGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCT                                                                                   <  SRR4302533.5706565/94‑1 (MQ=60)

            TGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCG                                                                                   >  SRR4302533.4156776/1‑94 (MQ=60)

            TGACGCAATACTGGCTGGGGGTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCCGCGCCT                                                                                   >  SRR4302533.1922197/1‑94 (MQ=60)

             GACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTG                                                                                  >  SRR4302533.3473094/1‑94 (MQ=60)

             GACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTG                                                                                  >  SRR4302533.5208946/1‑94 (MQ=60)

               CGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCC                                                                                >  SRR4302533.4590063/1‑94 (MQ=60)

                GCAATACTGCGTGGGGTTAAATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGCCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCG                                                                               <  SRR4302533.4187351/94‑1 (MQ=60)

                 CAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGC                                                                              >  SRR4302533.3116269/1‑94 (MQ=60)

                   ATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTTAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTG                                                                            >  SRR4302533.4658227/1‑94 (MQ=60)

                   ATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTG                                                                            >  SRR4302533.1597800/1‑94 (MQ=60)

                   ATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTG                                                                            >  SRR4302533.6176762/1‑94 (MQ=60)

                    TACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGT                                                                           <  SRR4302533.264050/94‑1 (MQ=60)

                     ACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTG                                                                          <  SRR4302533.294534/94‑1 (MQ=60)

                      CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGC                                                                         <  SRR4302533.4417901/94‑1 (MQ=60)

                       TGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCG                                                                        >  SRR4302533.2428217/1‑94 (MQ=60)

                        GGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGC                                                                       <  SRR4302533.1845463/94‑1 (MQ=60)

                          CTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAT                                                                     >  SRR4302533.3381042/1‑94 (MQ=60)

                           TGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATT                                                                    >  SRR4302533.2189498/1‑94 (MQ=60)

                            GGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTA                                                                   >  SRR4302533.2443631/1‑94 (MQ=60)

                            GGGGTGAGATTGTGGCGGTAGCTGCCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTA                                                                   <  SRR4302533.2604172/94‑1 (MQ=60)

                             GGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAA                                                                  <  SRR4302533.3158708/94‑1 (MQ=60)

                                TTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCC                                                               <  SRR4302533.2396645/94‑1 (MQ=60)

                                 TAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCC                                                              >  SRR4302533.4635236/1‑94 (MQ=60)

                                 TAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCC                                                              <  SRR4302533.4631212/94‑1 (MQ=60)

                                  AGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCATACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCG                                                             <  SRR4302533.4372222/94‑1 (MQ=60)

                                  AGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCG                                                             <  SRR4302533.5459883/94‑1 (MQ=60)

                                   GATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGC                                                            >  SRR4302533.4052247/1‑94 (MQ=60)

                                   GATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGC                                                            <  SRR4302533.75476/94‑1 (MQ=60)

                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGCCCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           <  SRR4302533.5347162/94‑1 (MQ=60)

                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           >  SRR4302533.3485702/1‑94 (MQ=60)

                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           >  SRR4302533.1029787/1‑94 (MQ=60)

                                    ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           >  SRR4302533.1508447/1‑94 (MQ=60)

                                      TGTGGCGGTAGCTGGCTGAAAGCCGGGCTGGATGACCGCCAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGGCCGCAGC                                                         >  SRR4302533.2599576/1‑94 (MQ=60)

                                       GTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCC                                                        <  SRR4302533.2912446/94‑1 (MQ=60)

                                        GGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCA                                                       <  SRR4302533.1398871/94‑1 (MQ=60)

                                            GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCT                                                   <  SRR4302533.945170/94‑1 (MQ=60)

                                            GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCT                                                   >  SRR4302533.296788/1‑94 (MQ=60)

                                            GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGAT                                                   >  SRR4302533.1184360/1‑94 (MQ=60)

                                             GTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCGGGCTG                                                  <  SRR4302533.5487802/94‑1 (MQ=60)

                                             GTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTG                                                  >  SRR4302533.1697974/1‑94 (MQ=60)

                                              TAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGG                                                 >  SRR4302533.6014396/1‑94 (MQ=60)

                                               AGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                <  SRR4302533.3108252/94‑1 (MQ=60)

                                                GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               <  SRR4302533.1630510/94‑1 (MQ=60)

                                                GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               <  SRR4302533.2080673/94‑1 (MQ=60)

                                                 CTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCA                                              <  SRR4302533.6278430/94‑1 (MQ=60)

                                                 CTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCA                                              >  SRR4302533.1553654/1‑94 (MQ=60)

                                                  TGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAG                                             >  SRR4302533.2788492/1‑94 (MQ=60)

                                                  TGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAG                                             >  SRR4302533.3767293/1‑94 (MQ=60)

                                                    GCTGAAAGCGGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAG                                           <  SRR4302533.162279/94‑1 (MQ=60)

                                                    GCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAG                                           <  SRR4302533.871326/94‑1 (MQ=60)

                                                    GCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAG                                           >  SRR4302533.1916504/1‑94 (MQ=60)

                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302533.2062499/94‑1 (MQ=60)

                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302533.4808468/94‑1 (MQ=60)

                                                     CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302533.3277971/94‑1 (MQ=60)

                                                      TGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCG                                         >  SRR4302533.3569205/1‑94 (MQ=60)

                                                      TGAAAGCCGGGCTGTATGACCGAGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCG                                         <  SRR4302533.196628/94‑1 (MQ=60)

                                                        AAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCG                                       <  SRR4302533.2047804/94‑1 (MQ=60)

                                                        AAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCG                                       <  SRR4302533.5483612/94‑1 (MQ=60)

                                                          AGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGAT                                     <  SRR4302533.4674976/94‑1 (MQ=60)

                                                          AGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGAT                                     >  SRR4302533.5207366/1‑94 (MQ=60)

                                                           GCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATA                                    <  SRR4302533.5897574/94‑1 (MQ=60)

                                                           GCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATA                                    >  SRR4302533.3995693/1‑94 (MQ=60)

                                                             CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  <  SRR4302533.1108677/94‑1 (MQ=60)

                                                             CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  >  SRR4302533.1575237/1‑94 (MQ=60)

                                                             CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  <  SRR4302533.1962138/94‑1 (MQ=60)

                                                              GGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGG                                 <  SRR4302533.4751165/94‑1 (MQ=60)

                                                              GGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGG                                 <  SRR4302533.3106926/94‑1 (MQ=60)

                                                              GGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGG                                 >  SRR4302533.126282/1‑94 (MQ=60)

                                                                GCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               <  SRR4302533.2448694/94‑1 (MQ=60)

                                                                GCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               <  SRR4302533.1637319/94‑1 (MQ=60)

                                                                GCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               >  SRR4302533.6092224/1‑94 (MQ=60)

                                                                GCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               >  SRR4302533.5352888/1‑94 (MQ=60)

                                                                 CTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAG                              >  SRR4302533.370910/1‑94 (MQ=60)

                                                                  TGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGA                             >  SRR4302533.4557421/1‑94 (MQ=60)

                                                                  TGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGA                             >  SRR4302533.2903152/1‑94 (MQ=60)

                                                                   GTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAA                            <  SRR4302533.745039/94‑1 (MQ=60)

                                                                   GTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATCGCAGAA                            >  SRR4302533.2234462/1‑94 (MQ=60)

                                                                    TATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAC                           <  SRR4302533.3872605/94‑1 (MQ=60)

                                                                     ATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACT                          <  SRR4302533.190524/94‑1 (MQ=60)

                                                                      TGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTG                         <  SRR4302533.3965046/94‑1 (MQ=60)

                                                                       GACCGGGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGT                        <  SRR4302533.5846208/94‑1 (MQ=60)

                                                                       GACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGT                        >  SRR4302533.5092009/1‑94 (MQ=60)

                                                                       GACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGT                        >  SRR4302533.5092010/1‑94 (MQ=60)

                                                                       GACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGT                        >  SRR4302533.6194246/1‑94 (MQ=60)

                                                                        ACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTG                       <  SRR4302533.5124748/94‑1 (MQ=60)

                                                                         CCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGG                      <  SRR4302533.3128214/94‑1 (MQ=60)

                                                                          CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGC                     <  SRR4302533.2642297/94‑1 (MQ=60)

                                                                          CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGC                     <  SRR4302533.3537674/94‑1 (MQ=60)

                                                                          CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGC                     >  SRR4302533.6209682/1‑94 (MQ=60)

                                                                           GCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCA                    <  SRR4302533.4501579/94‑1 (MQ=60)

                                                                           GCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCA                    <  SRR4302533.5600856/94‑1 (MQ=60)

                                                                            CGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAA                   >  SRR4302533.5666916/1‑94 (MQ=60)

                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  <  SRR4302533.5899772/94‑1 (MQ=60)

                                                                             GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  >  SRR4302533.6283199/1‑94 (MQ=60)

                                                                              AAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCCGAGCGCGATATGGCAGAACTGTGGCAATG                 >  SRR4302533.2821938/1‑94 (MQ=60)

                                                                              AAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATG                 >  SRR4302533.40648/1‑94 (MQ=60)

                                                                              AAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACAGTGGGAATG                 >  SRR4302533.4954880/1‑94 (MQ=60)

                                                                               AGGCCGGGAGGGAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGC                <  SRR4302533.3830508/94‑1 (MQ=60)

                                                                               AGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGC                <  SRR4302533.3660734/94‑1 (MQ=60)

                                                                                 GCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTG              <  SRR4302533.1481150/94‑1 (MQ=60)

                                                                                  CCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGCGCTATGGCAGAACTGTGGCACTGCTGC             >  SRR4302533.1229051/1‑94 (MQ=60)

                                                                                   CGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGatggcatgccgca            >  SRR4302533.3653346/1‑81 (MQ=60)

                                                                                   CGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCA            <  SRR4302533.803583/94‑1 (MQ=60)

                                                                                    GGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAT           <  SRR4302533.3525911/94‑1 (MQ=60)

                                                                                      GAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGG         <  SRR4302533.1223349/94‑1 (MQ=60)

                                                                                       AGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGC        <  SRR4302533.5029726/94‑1 (MQ=60)

                                                                                        GGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCT       <  SRR4302533.1463917/94‑1 (MQ=60)

                                                                                         GCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTG      <  SRR4302533.4698109/94‑1 (MQ=60)

                                                                                           AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAGAGCGCGATATGGCAGAACTGAGGCAATGGCGCATGGCTGGC    >  SRR4302533.3878441/1‑94 (MQ=60)

                                                                                           AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTC    >  SRR4302533.3207870/1‑94 (MQ=60)

                                                                                           AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTC    <  SRR4302533.1181264/94‑1 (MQ=60)

                                                                                            GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA   >  SRR4302533.138707/1‑94 (MQ=60)

                                                                                            GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA   >  SRR4302533.3113001/1‑94 (MQ=60)

                                                                                             ACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  <  SRR4302533.5944412/94‑1 (MQ=60)

                                                                                             ACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  <  SRR4302533.1904972/94‑1 (MQ=60)

TGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  >  NC_000913/3744703‑3744889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`