Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,649,014 C→A 37.4% D129Y (GAT→TAT)  rspA ← bifunctional D‑altronate/D‑mannonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,649,0140CA37.4% 366.9 / 258.7 251D129Y (GAT→TAT) rspAbifunctional D‑altronate/D‑mannonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (75/82);  new base A (38/55);  total (113/138)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 263 total aligned reads displayed.

CGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAACCGGAC  >  CP009273/1648865‑1649163
                                                                                                                                                     |                                                                                                                                                     
cGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTtcatc                                                                                                                                                       >  1:1943311/1‑150 (MQ=255)
   ccACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAAt                                                                                                                                                    <  2:3662634/150‑1 (MQ=255)
   ccACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAAt                                                                                                                                                    <  1:3382078/150‑1 (MQ=255)
    cACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATa                                                                                                                                                   <  2:3622133/150‑1 (MQ=255)
    cACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATa                                                                                                                                                   <  1:2059944/150‑1 (MQ=255)
      cTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACt                                                                                                                                                 <  2:591398/150‑1 (MQ=255)
       tGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTTTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACtg                                                                                                                                                <  2:1929162/150‑1 (MQ=255)
        gTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACtgt                                                                                                                                               >  2:3199859/1‑150 (MQ=255)
          ccTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGa                                                                                                                                             <  2:2252278/150‑1 (MQ=255)
           cTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGAc                                                                                                                                            >  1:4435938/1‑150 (MQ=255)
           cTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGAc                                                                                                                                            >  1:2140281/1‑150 (MQ=255)
                  tGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCggtggt                                                                                                                                     >  1:3065786/1‑150 (MQ=255)
                  tGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCggtggt                                                                                                                                     <  1:2276083/150‑1 (MQ=255)
                    cGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTAt                                                                                                                                   <  2:1740745/150‑1 (MQ=255)
                    cGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTAt                                                                                                                                   <  2:4070109/150‑1 (MQ=255)
                    cGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATAATCCAGAGCTTCATCAATACTGTGACCGGTGGTAt                                                                                                                                   <  2:489555/150‑1 (MQ=255)
                     gggTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATg                                                                                                                                  <  2:1482157/150‑1 (MQ=255)
                     gggTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATg                                                                                                                                  <  2:1482166/150‑1 (MQ=255)
                          cATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAAt                                                                                                                             <  1:2804534/150‑1 (MQ=255)
                                ccAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCa                                                                                                                       >  2:3176494/1‑150 (MQ=255)
                                 cAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCAt                                                                                                                      >  1:797025/1‑150 (MQ=255)
                                   gACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCa                                                                                                                    >  2:2153267/1‑150 (MQ=255)
                                    acctttacctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCAc                                                                                                                   <  1:927055/150‑1 (MQ=255)
                                    acctttacctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCAc                                                                                                                   >  1:2837303/1‑150 (MQ=255)
                                      ctttacctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCAccc                                                                                                                 <  2:3144822/150‑1 (MQ=255)
                                      ctttacctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCAccc                                                                                                                 <  2:3984849/150‑1 (MQ=255)
                                      ctttacctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCAccc                                                                                                                 <  2:556997/150‑1 (MQ=255)
                                         tacctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCtt                                                                                                              >  2:2812386/1‑150 (MQ=255)
                                          acctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTc                                                                                                             <  2:1832107/150‑1 (MQ=255)
                                          acctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTc                                                                                                             <  1:3353493/150‑1 (MQ=255)
                                          acctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTc                                                                                                             >  1:4053661/1‑150 (MQ=255)
                                           cctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCa                                                                                                            >  1:4027097/1‑150 (MQ=255)
                                            ctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCAc                                                                                                           >  1:1394180/1‑150 (MQ=255)
                                            ctttCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTTTTATGGCAATAAACCATAACCCCTTCAc                                                                                                           <  2:160862/150‑1 (MQ=255)
                                                cGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACgaga                                                                                                       >  2:3280286/1‑150 (MQ=255)
                                                 gACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGAc                                                                                                      <  2:4481138/150‑1 (MQ=255)
                                                 gACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGAc                                                                                                      <  2:1922678/150‑1 (MQ=255)
                                                  aCATGCCGAAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGAcg                                                                                                     >  2:3248636/1‑150 (MQ=255)
                                                      gCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGcgcc                                                                                                 <  1:457998/150‑1 (MQ=255)
                                                      gCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGcgcc                                                                                                 <  1:1776948/150‑1 (MQ=255)
                                                       ccGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGcgccg                                                                                                >  1:2100721/1‑150 (MQ=255)
                                                       ccGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGcgccg                                                                                                <  1:3943612/150‑1 (MQ=255)
                                                         gTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCgccgcc                                                                                              >  2:2897517/1‑150 (MQ=255)
                                                          tAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGccgccg                                                                                             >  1:1747798/1‑150 (MQ=255)
                                                          tAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGccgccg                                                                                             >  2:4202651/1‑150 (MQ=255)
                                                          tAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGccgccg                                                                                             >  1:4617889/1‑150 (MQ=255)
                                                           aGGTGGTTTTCATACCAGGGATTCCGCACTNCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGa                                                                                            >  1:4284689/1‑150 (MQ=255)
                                                            ggtggtTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAg                                                                                           >  2:3769730/1‑150 (MQ=255)
                                                             gtggtTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGt                                                                                          <  2:2420929/150‑1 (MQ=255)
                                                               ggtTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTaa                                                                                        >  2:3019013/1‑150 (MQ=255)
                                                                   ttCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTgg                                                                                    <  1:2929785/150‑1 (MQ=255)
                                                                       tACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTaaa                                                                                <  2:3329253/150‑1 (MQ=255)
                                                                       tACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTaaa                                                                                >  1:4154234/1‑150 (MQ=255)
                                                                         ccAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGc                                                                              <  1:1337183/150‑1 (MQ=255)
                                                                          cAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCg                                                                             >  2:275582/1‑150 (MQ=255)
                                                                          cAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCg                                                                             <  2:829362/150‑1 (MQ=255)
                                                                           aGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCgg                                                                            >  1:2774154/1‑150 (MQ=255)
                                                                            gggATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATTAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGc                                                                           >  2:3559868/1‑150 (MQ=255)
                                                                            gggATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGc                                                                           <  1:6942/150‑1 (MQ=255)
                                                                                ttCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGt                                                                       >  1:3004689/1‑150 (MQ=255)
                                                                                 tCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGtt                                                                      <  2:823807/150‑1 (MQ=255)
                                                                                    gCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCATTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGc                                                                   >  1:2025149/1‑150 (MQ=255)
                                                                                     cACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCa                                                                  <  1:683931/150‑1 (MQ=255)
                                                                                               ggATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCtt                                                        <  2:329791/150‑1 (MQ=255)
                                                                                                gATGGCTTTGAATCCAAGCTCTTGATGAAGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCttt                                                       <  1:1825346/150‑1 (MQ=255)
                                                                                                 aTGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGCTAAAGCGGCATGTTGGCAGCTTTGGCTTTa                                                      <  1:3892262/150‑1 (MQ=255)
                                                                                                  tGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTaa                                                     >  1:77046/1‑150 (MQ=255)
                                                                                                   ggCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAat                                                    <  1:1551905/150‑1 (MQ=255)
                                                                                                   ggCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAat                                                    <  1:475687/150‑1 (MQ=255)
                                                                                                   ggCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAat                                                    <  1:1079989/150‑1 (MQ=255)
                                                                                                   ggCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAat                                                    <  1:1080039/150‑1 (MQ=255)
                                                                                                     cTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAatat                                                  <  2:4154234/150‑1 (MQ=255)
                                                                                                       ttGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATcc                                                <  1:3252102/150‑1 (MQ=255)
                                                                                                       ttGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATcc                                                >  2:3480941/1‑150 (MQ=255)
                                                                                                           aTCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCcaca                                            <  1:2523339/150‑1 (MQ=255)
                                                                                                            tCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAg                                           <  2:931493/150‑1 (MQ=255)
                                                                                                             ccAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAgc                                          >  1:3968474/1‑150 (MQ=255)
                                                                                                             ccAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAgc                                          <  1:825596/150‑1 (MQ=255)
                                                                                                                 gCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCa                                      >  2:1311589/1‑150 (MQ=255)
                                                                                                                  ctctTGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCat                                     >  2:4511161/1‑150 (MQ=255)
                                                                                                                    ctTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCatat                                   <  1:3744901/150‑1 (MQ=255)
                                                                                                                    ctTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCatat                                   >  2:4372842/1‑150 (MQ=255)
                                                                                                                     ttGATGACGGGCATAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATc                                  >  2:432610/1‑150 (MQ=255)
                                                                                                                             gggCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTg                          >  2:158716/1‑150 (MQ=255)
                                                                                                                                  tAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATg                     >  1:199769/1‑150 (MQ=255)
                                                                                                                                  tAATCATCCAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATg                     >  1:4020797/1‑150 (MQ=255)
                                                                                                                                  tAATCATCCAGAGCTTAATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATg                     <  1:2603129/150‑1 (MQ=255)
                                                                                                                                         ccAGAGCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACa              >  1:3763356/1‑150 (MQ=255)
                                                                                                                                           agagCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATc            <  1:920987/150‑1 (MQ=255)
                                                                                                                                            gagCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCg           >  1:597492/1‑150 (MQ=255)
                                                                                                                                            gagCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCg           <  2:15631/150‑1 (MQ=255)
                                                                                                                                            gagCTTCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCg           <  2:1702117/150‑1 (MQ=255)
                                                                                                                                             agCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGt          >  2:2834949/1‑150 (MQ=255)
                                                                                                                                                ttCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGATTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAAc       <  2:4366467/150‑1 (MQ=255)
                                                                                                                                                ttCATAAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAAc       >  2:3519140/1‑150 (MQ=255)
                                                                                                                                                 tcatcaATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAAcc      >  2:4276609/1‑150 (MQ=255)
                                                                                                                                                 tcatcaATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAAcc      <  2:2644198/150‑1 (MQ=255)
                                                                                                                                                 tcatcaATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAAcc      <  1:3540545/150‑1 (MQ=255)
                                                                                                                                                  catcaATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAACCg     >  1:4269747/1‑150 (MQ=255)
                                                                                                                                                     caATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAACCGGAc  >  2:648324/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCCACTGTCCTTTGGTTGCGGGTTCATAAGCCAGACCTTTACCTTTCGACATGCCGTAGGTGGTTTTCATACCAGGGATTCCGCACTGCACGCGGATGGCTTTGAATCCAAGCTCTTGATGACGGGCATAATCATCCAGAGCTTCATCAATACTGTGACCGGTGGTATGGCAATAAACCATCACCCCTTCACGAGACGCGCCGCCGAGTAACTGGTAAAGCGGCATGTTGGCAGCTTTGGCTTTAATATCCCACAGCGCCATATCAACCGCTGAAATGGCCGACATCGTAACCGGAC  >  CP009273/1648865‑1649163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A