Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,675,777 C→A 16.0% S182* (TCG→TAG)  folM → dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,675,7770CA16.0% 867.8 / 93.6 271S182* (TCG→TAG) folMdihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme
Reads supporting (aligned to +/- strand):  ref base C (108/119);  new base A (18/26);  total (126/145)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

CGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTC  >  CP009273/1675628‑1675925
                                                                                                                                                     |                                                                                                                                                    
cGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATc                                                                                                                                                      <  1:2920543/150‑1 (MQ=255)
cGAGATCATTCACTTTACCGATTCTGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCACAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATc                                                                                                                                                      <  2:2857003/150‑1 (MQ=255)
 gATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCg                                                                                                                                                     >  2:3982774/1‑150 (MQ=255)
   tatCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCt                                                                                                                                                   <  2:1772920/150‑1 (MQ=255)
   tatCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCt                                                                                                                                                   >  2:3282109/1‑150 (MQ=255)
     tCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAANTGAAAGTGAATTCTATTGCGCCATAGCTGa                                                                                                                                                 <  1:4280011/150‑1 (MQ=255)
      cATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGAt                                                                                                                                                <  2:4304674/150‑1 (MQ=255)
       aTTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATc                                                                                                                                               >  1:1002935/1‑150 (MQ=255)
        ttCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATcc                                                                                                                                              <  1:366872/150‑1 (MQ=255)
        ttCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATcc                                                                                                                                              <  1:2719230/150‑1 (MQ=255)
          cACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTg                                                                                                                                            <  1:2818404/150‑1 (MQ=255)
              ttACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTa                                                                                                                                        >  2:726300/1‑150 (MQ=255)
                 ccGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATg                                                                                                                                     >  2:294958/1‑150 (MQ=255)
                  cGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGa                                                                                                                                    >  1:868971/1‑150 (MQ=255)
                    aTTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAAc                                                                                                                                  >  2:1641245/1‑150 (MQ=255)
                       aTGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatg                                                                                                                               >  1:1672602/1‑150 (MQ=255)
                       aTGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatg                                                                                                                               >  2:2954696/1‑150 (MQ=255)
                       aTGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACatg                                                                                                                               <  1:95793/150‑1 (MQ=255)
                           ggtggAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatga                                                                                                                           >  2:1102672/1‑150 (MQ=255)
                            gtggAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACatgatgat                                                                                                                          <  1:3135408/150‑1 (MQ=255)
                              ggAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGc                                                                                                                        >  2:61540/1‑150 (MQ=255)
                               gAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGcc                                                                                                                       <  2:978320/150‑1 (MQ=255)
                                  cgcgGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGaa                                                                                                                    <  2:2083613/150‑1 (MQ=255)
                                  cgcgGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGaa                                                                                                                    <  2:4413989/150‑1 (MQ=255)
                                   gcgGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAat                                                                                                                   <  1:377892/150‑1 (MQ=255)
                                     ggTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAatat                                                                                                                 >  1:4343747/1‑150 (MQ=255)
                                       tAGCGACAAACATATTGCGTATGCTGCACGCAAAGCGGCACTGGATTATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCg                                                                                                               <  1:427078/150‑1 (MQ=255)
                                       tAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCg                                                                                                               <  1:427453/150‑1 (MQ=255)
                                         gCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGac                                                                                                             <  2:4481438/150‑1 (MQ=255)
                                          cGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGaca                                                                                                            >  1:4453917/1‑150 (MQ=255)
                                            aCAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGacaac                                                                                                          <  1:1026213/150‑1 (MQ=255)
                                             cAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGacaaca                                                                                                         <  2:550175/150‑1 (MQ=255)
                                                 cATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGgcg                                                                                                     >  2:3924328/1‑150 (MQ=255)
                                                    atTGCGTATGCTGCAAGCAAAGCGGCACGGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTg                                                                                                  <  1:1351948/150‑1 (MQ=255)
                                                      tGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGaa                                                                                                <  1:3883120/150‑1 (MQ=255)
                                                       gCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAt                                                                                               >  2:1613481/1‑150 (MQ=255)
                                                        cGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATa                                                                                              <  1:4220947/150‑1 (MQ=255)
                                                            tgctgcAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATc                                                                                          <  1:2305455/150‑1 (MQ=255)
                                                                  aagcaaAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACtgat                                                                                    <  1:741321/150‑1 (MQ=255)
                                                                    gcaaAGCGGCTCTGGATTATTTGACCCGATCGTTTGCCCGCAAGATGGCACCGGAAGTGTAAGTGAATTCTATTGCGACATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCTCtgatga                                                                                  <  1:1069884/150‑1 (MQ=255)
                                                                     caaAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa                                                                                 <  2:2272680/150‑1 (MQ=255)
                                                                     caaAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaa                                                                                 <  2:2456346/150‑1 (MQ=255)
                                                                       aaGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaaa                                                                               >  1:626987/1‑150 (MQ=255)
                                                                       aaGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGaaaa                                                                               >  2:3581081/1‑150 (MQ=255)
                                                                         gCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAAcc                                                                             <  2:2742334/150‑1 (MQ=255)
                                                                          cGGCTCTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCTGAAGTGATAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACcg                                                                            <  1:1423573/150‑1 (MQ=255)
                                                                             cACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCgcgc                                                                         >  2:2013778/1‑150 (MQ=255)
                                                                               cTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCt                                                                       <  1:3540229/150‑1 (MQ=255)
                                                                                 ggtttttatGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTTTTGCGGCATCGCTGTTCCTGTTTAATGAAGTGGATGATGGGGTAGATCGACAACAGGCGCTGTATTTATCACTGATGAATTCCGCGCCTgg                                                                     <  2:382901/144‑1 (MQ=255)
                                                                                    taataTGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCga                                                                  >  1:2366524/1‑150 (MQ=255)
                                                                                     aataTGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgag                                                                 >  2:960196/1‑150 (MQ=255)
                                                                                     aataTGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgag                                                                 >  1:4595055/1‑150 (MQ=255)
                                                                                      atatGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgaga                                                                >  1:4198608/1‑150 (MQ=255)
                                                                                      atatGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCgaga                                                                <  1:292094/150‑1 (MQ=255)
                                                                                             ccGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGt                                                         <  2:1343243/150‑1 (MQ=255)
                                                                                              cGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTg                                                        <  1:4118352/150‑1 (MQ=255)
                                                                                              cGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTg                                                        <  1:2395394/150‑1 (MQ=255)
                                                                                               gCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGa                                                       >  2:487486/1‑150 (MQ=255)
                                                                                               gCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGa                                                       >  1:3852591/1‑150 (MQ=255)
                                                                                                   gTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGa                                                   >  1:4480931/1‑150 (MQ=255)
                                                                                                     ttGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGAcc                                                 <  1:3043190/150‑1 (MQ=255)
                                                                                                        cccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg                                              <  1:3429064/150‑1 (MQ=255)
                                                                                                        cccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg                                              <  1:3380938/150‑1 (MQ=255)
                                                                                                         ccGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGt                                             <  1:1571872/150‑1 (MQ=255)
                                                                                                          cGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc                                            >  2:1799050/1‑150 (MQ=255)
                                                                                                          cGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc                                            <  2:3141498/150‑1 (MQ=255)
                                                                                                          cGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc                                            >  2:262812/1‑150 (MQ=255)
                                                                                                          cGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc                                            <  2:1961556/150‑1 (MQ=255)
                                                                                                             aaGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAt                                         >  2:2810359/1‑150 (MQ=255)
                                                                                                             aaGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAt                                         >  2:2127922/1‑150 (MQ=255)
                                                                                                               gCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAtta                                       <  2:3054980/150‑1 (MQ=255)
                                                                                                                 tGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttact                                     >  2:1122104/1‑150 (MQ=255)
                                                                                                                 tGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttact                                     >  2:1959448/1‑150 (MQ=255)
                                                                                                                  ggCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttactt                                    >  2:3511579/1‑150 (MQ=255)
                                                                                                                    cACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttac                                  <  1:3982774/150‑1 (MQ=255)
                                                                                                                    cACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttac                                  <  2:1238626/150‑1 (MQ=255)
                                                                                                                     aCCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttact                                 >  1:9970/1‑150 (MQ=255)
                                                                                                                     aCCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACACGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttact                                 >  2:341773/1‑150 (MQ=255)
                                                                                                                          aagtgaaagtgaATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCa                            <  1:16628/150‑1 (MQ=255)
                                                                                                                           agtgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAg                           <  1:1838936/150‑1 (MQ=255)
                                                                                                                            gtgaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGt                          <  1:1757556/150‑1 (MQ=255)
                                                                                                                              gaaagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTg                        >  2:2031621/1‑150 (MQ=255)
                                                                                                                                aagtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCt                      <  1:1991220/150‑1 (MQ=255)
                                                                                                                                 agtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCtt                     >  2:433927/1‑150 (MQ=255)
                                                                                                                                 agtgaaTTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCtt                     <  2:1078186/150‑1 (MQ=255)
                                                                                                                                 agtgaaTTCTATTGCGCCATAGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCtt                     <  2:1512651/150‑1 (MQ=255)
                                                                                                                                  gtgaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCttt                    <  1:4140048/150‑1 (MQ=255)
                                                                                                                                    gaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGt                  >  2:4435185/1‑150 (MQ=255)
                                                                                                                                    gaaTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGt                  <  1:3709811/150‑1 (MQ=255)
                                                                                                                                      aTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCa                >  2:3713935/1‑150 (MQ=255)
                                                                                                                                      aTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCa                <  2:4345236/150‑1 (MQ=255)
                                                                                                                                       ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCAc               <  1:3077504/150‑1 (MQ=255)
                                                                                                                                         cTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCg             <  2:4174948/150‑1 (MQ=255)
                                                                                                                                           aTTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGa           >  2:4236245/1‑150 (MQ=255)
                                                                                                                                           aTTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGa           >  2:1904800/1‑150 (MQ=255)
                                                                                                                                                gcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCTATTACATACATACCAGTTGCTTTGTCACCGGACGCaa      >  1:1498746/1‑149 (MQ=255)
                                                                                                                                                gcCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAg      <  1:1102672/150‑1 (MQ=255)
                                                                                                                                                  cATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGtt    <  2:3896099/150‑1 (MQ=255)
                                                                                                                                                  cATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGtt    >  2:837725/1‑150 (MQ=255)
                                                                                                                                                    tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTc  >  2:3021515/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGCTTTGTCACCGGACGCAGTTTC  >  CP009273/1675628‑1675925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A