Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,832,413 C→A 100% L315L (CTC→CTA)  ynjC → inner membrane putative ABC superfamily transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,832,4130CA96.5% 823.1 / 19.5 256L315L (CTC→CTA) ynjCinner membrane putative ABC superfamily transporter permease
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base A (144/103);  total (150/106)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 269 total aligned reads displayed.

TATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTA  >  CP009273/1832266‑1832559
                                                                                                                                                   |                                                                                                                                                  
tATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaat                                                                                                                                                  <  1:3177662/150‑1 (MQ=255)
tATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaat                                                                                                                                                  >  1:2887701/1‑150 (MQ=255)
 aTTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaatc                                                                                                                                                 <  1:3435168/150‑1 (MQ=255)
 aTTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaatc                                                                                                                                                 <  1:176098/150‑1 (MQ=255)
  ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGTCGATCAAGAGTGAAGCGCtaatca                                                                                                                                                >  2:2349267/1‑150 (MQ=255)
  ttCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCtaatca                                                                                                                                                <  2:4411246/150‑1 (MQ=255)
   tCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCaa                                                                                                                                               >  2:978080/1‑150 (MQ=255)
    ccccGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAAc                                                                                                                                              <  1:2894149/150‑1 (MQ=255)
       cgcgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGc                                                                                                                                           >  1:4494383/1‑150 (MQ=255)
         cgTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCt                                                                                                                                         >  2:3366107/1‑150 (MQ=255)
          gTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTg                                                                                                                                        >  1:3732999/1‑150 (MQ=255)
           tAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCGGGGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGACGCGCTAATCAACAGCCTGa                                                                                                                                       >  2:2034972/1‑150 (MQ=255)
                ggCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATg                                                                                                                                  >  2:1517697/1‑150 (MQ=255)
                 gCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATgg                                                                                                                                 <  1:3632166/150‑1 (MQ=255)
                 gCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATgg                                                                                                                                 <  1:1308084/150‑1 (MQ=255)
                   gTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATgggg                                                                                                                               >  2:614627/1‑150 (MQ=255)
                      cGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCtg                                                                                                                            <  1:4412340/150‑1 (MQ=255)
                        cAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTTCCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGTCGATCCTCGCGGTTCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCtggt                                                                                                                          <  1:443237/150‑1 (MQ=255)
                        cAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAAAGGGGCtggt                                                                                                                          >  1:2748765/1‑150 (MQ=255)
                         aaGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCgggtg                                                                                                                         >  2:4265510/1‑150 (MQ=255)
                          aGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGGGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCGAATCAACAGCCTGACAATGGGGCtggtgg                                                                                                                        >  2:486172/1‑150 (MQ=255)
                                 cACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGGGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGAGCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCGGGTGGCGACAtt                                                                                                                 >  2:679742/1‑150 (MQ=255)
                                  aCGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTc                                                                                                                >  1:4096570/1‑150 (MQ=255)
                                  aCGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTc                                                                                                                >  1:1454038/1‑150 (MQ=255)
                                    gCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCAt                                                                                                              <  1:1706422/150‑1 (MQ=255)
                                    gCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGGGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCAt                                                                                                              >  2:1155411/1‑150 (MQ=255)
                                       tttATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGc                                                                                                           <  2:1675231/150‑1 (MQ=255)
                                          aTTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCttt                                                                                                        >  1:3512603/1‑150 (MQ=255)
                                           ttGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAAACGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTTAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTg                                                                                                       >  1:2573615/1‑150 (MQ=255)
                                                 ggCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTg                                                                                                 <  2:4583456/150‑1 (MQ=255)
                                                  gCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGt                                                                                                <  1:4441422/150‑1 (MQ=255)
                                                  gCAATACGCTGGCGA‑TTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGtt                                                                                               >  1:626365/1‑150 (MQ=255)
                                                     aTACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTAc                                                                                             <  1:1213262/150‑1 (MQ=255)
                                                      tACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGTGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTAct                                                                                            <  1:1652389/150‑1 (MQ=255)
                                                        cGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTActgc                                                                                          >  2:4220008/1‑150 (MQ=255)
                                                           tGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTAGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCtgt                                                                                       >  2:4179779/1‑150 (MQ=255)
                                                               gATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCt                                                                                   >  1:3493987/1‑150 (MQ=255)
                                                               gATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCt                                                                                   >  1:805883/1‑150 (MQ=255)
                                                               gATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCt                                                                                   >  1:2609848/1‑150 (MQ=255)
                                                                aTTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTg                                                                                  >  2:2665293/1‑150 (MQ=255)
                                                                 tttttttACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTgg                                                                                 <  1:1133744/150‑1 (MQ=255)
                                                                        aCCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCACCAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATgggg                                                                          <  2:1277753/150‑1 (MQ=255)
                                                                         cccTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATggggg                                                                         >  2:3224256/1‑150 (MQ=255)
                                                                         cccTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATggggg                                                                         >  1:988856/1‑150 (MQ=255)
                                                                          ccTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGc                                                                        <  2:2619348/150‑1 (MQ=255)
                                                                          ccTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGc                                                                        <  2:301464/150‑1 (MQ=255)
                                                                           cTTACCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGACGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGcc                                                                       <  2:2615044/150‑1 (MQ=255)
                                                                             tAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCcac                                                                     >  2:669959/1‑150 (MQ=255)
                                                                             tAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCcac                                                                     >  2:3420951/1‑150 (MQ=255)
                                                                               aCCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAg                                                                   <  2:4494383/150‑1 (MQ=255)
                                                                                ccGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGc                                                                  >  1:2343837/1‑150 (MQ=255)
                                                                                  ggTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGt                                                                >  2:717341/1‑150 (MQ=255)
                                                                                       gCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCa                                                           <  2:1113787/150‑1 (MQ=255)
                                                                                       gCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCa                                                           <  1:2680260/150‑1 (MQ=255)
                                                                                        cTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAg                                                          >  2:2313493/1‑150 (MQ=255)
                                                                                           tgtgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTtg                                                       <  2:463779/150‑1 (MQ=255)
                                                                                            gtgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTtgt                                                      <  1:416917/150‑1 (MQ=255)
                                                                                             tgtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTtgtg                                                     <  2:4070443/150‑1 (MQ=255)
                                                                                              gtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTgg                                                    >  2:1348946/1‑150 (MQ=255)
                                                                                              gtgGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTgg                                                    >  2:3525206/1‑150 (MQ=255)
                                                                                                  ttCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTAt                                                >  2:2164606/1‑150 (MQ=255)
                                                                                                  ttCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTAt                                                >  1:668264/1‑150 (MQ=255)
                                                                                                     tgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGt                                             <  1:2632546/150‑1 (MQ=255)
                                                                                                     tgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGt                                             <  1:4620860/150‑1 (MQ=255)
                                                                                                     tgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGt                                             >  2:1764284/1‑150 (MQ=255)
                                                                                                     tgctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGt                                             >  1:3308138/1‑150 (MQ=255)
                                                                                                      gctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGtt                                            <  2:2544938/150‑1 (MQ=255)
                                                                                                      gctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGtt                                            <  1:1182560/150‑1 (MQ=255)
                                                                                                       ctgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCACTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTg                                           <  2:3740492/150‑1 (MQ=255)
                                                                                                        tgGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGc                                          <  2:2893257/150‑1 (MQ=255)
                                                                                                           cGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCa                                       <  1:1529652/150‑1 (MQ=255)
                                                                                                               ccTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCAtttt                                   >  2:3938841/1‑150 (MQ=255)
                                                                                                               ccTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCAtttt                                   >  1:4215497/1‑150 (MQ=255)
                                                                                                                cTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTtta                                  >  2:4111219/1‑150 (MQ=255)
                                                                                                                cTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTtta                                  >  1:1905455/1‑150 (MQ=255)
                                                                                                                 tCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTttat                                 <  2:1408118/150‑1 (MQ=255)
                                                                                                                 tCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTttat                                 <  2:3732999/150‑1 (MQ=255)
                                                                                                                  cgcgGATAAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTttatt                                >  2:2732455/1‑150 (MQ=255)
                                                                                                                    cgGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAc                              >  2:3479775/1‑150 (MQ=255)
                                                                                                                    cgGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAc                              >  1:524599/1‑150 (MQ=255)
                                                                                                                    cgGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAc                              >  2:4081287/1‑150 (MQ=255)
                                                                                                                    cgGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAc                              >  1:1397270/1‑150 (MQ=255)
                                                                                                                      gATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCt                            <  1:102353/150‑1 (MQ=255)
                                                                                                                      gATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCt                            <  1:380787/150‑1 (MQ=255)
                                                                                                                           gTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGctct                       <  2:1300822/150‑1 (MQ=255)
                                                                                                                           gTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGctct                       <  1:2014206/150‑1 (MQ=255)
                                                                                                                                 gaTCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACt                 >  1:2469628/1‑150 (MQ=255)
                                                                                                                                 gaTCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACt                 <  1:2665293/150‑1 (MQ=255)
                                                                                                                                 gaTCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACt                 >  2:2188085/1‑150 (MQ=255)
                                                                                                                                   tCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtgg               <  1:2096745/150‑1 (MQ=255)
                                                                                                                                     aaTAGTGAAGCGCTAATCAACAGCCTGACCATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggtg             >  2:2455440/1‑150 (MQ=255)
                                                                                                                                     aaTAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACtggtg             <  1:3936839/150‑1 (MQ=255)
                                                                                                                                        aGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCg          >  2:2798079/1‑150 (MQ=255)
                                                                                                                                        aGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCg          >  1:3046848/1‑150 (MQ=255)
                                                                                                                                         gTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCgg         <  2:2096309/150‑1 (MQ=255)
                                                                                                                                         gTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCgg         <  2:3768436/150‑1 (MQ=255)
                                                                                                                                           gAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGc       >  2:4629322/1‑150 (MQ=255)
                                                                                                                                             aGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCa     >  2:3625335/1‑150 (MQ=255)
                                                                                                                                               cgcTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGt   >  2:2479865/1‑150 (MQ=255)
                                                                                                                                                gcTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTa  <  1:3366107/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTA  >  CP009273/1832266‑1832559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTA  >  CP009273/1832264‑1832559
                                                                                                                                                     |                                                                                                                                                  
ACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGCTCAGTCGACGATCAATAGTGAAGCGCTA                                                                                                                                                    >  SRR19973072.2767391/1‑150 (MQ=60)
ACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTA                                                                                                                                                    >  SRR19973072.2767407/1‑150 (MQ=60)
ACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTA                                                                                                                                                    >  SRR19973072.2879862/1‑150 (MQ=60)
  TATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAAT                                                                                                                                                  <  SRR19973072.3184362/150‑1 (MQ=60)
  TATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAAT                                                                                                                                                  >  SRR19973072.2893891/1‑150 (MQ=60)
  TATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAAT                                                                                                                                                  >  SRR19973072.2893907/1‑150 (MQ=60)
   ATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATC                                                                                                                                                 <  SRR19973072.3442319/150‑1 (MQ=60)
   ATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATC                                                                                                                                                 >  SRR19973072.560078/1‑150 (MQ=60)
   ATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATC                                                                                                                                                 >  SRR19973072.560035/1‑150 (MQ=60)
   ATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATC                                                                                                                                                 <  SRR19973072.176546/150‑1 (MQ=60)
   ATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATC                                                                                                                                                 <  SRR19973072.176540/150‑1 (MQ=60)
   ATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATC                                                                                                                                                 <  SRR19973072.3442383/150‑1 (MQ=60)
      CCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAAC                                                                                                                                              <  SRR19973072.2900349/150‑1 (MQ=60)
      CCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAAC                                                                                                                                              <  SRR19973072.3923345/150‑1 (MQ=60)
       CCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACA                                                                                                                                             <  SRR19973072.45358/150‑1 (MQ=60)
         CGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGC                                                                                                                                           >  SRR19973072.4503877/1‑150 (MQ=60)
           CGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCT                                                                                                                                         >  SRR19973072.7787/1‑150 (MQ=60)
            GTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTG                                                                                                                                        >  SRR19973072.3740853/1‑150 (MQ=60)
            GTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTG                                                                                                                                        >  SRR19973072.1411183/1‑150 (MQ=60)
              AGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGAC                                                                                                                                      <  SRR19973072.3232870/150‑1 (MQ=60)
                 TGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAAT                                                                                                                                   <  SRR19973072.4213945/150‑1 (MQ=60)
                   GCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGG                                                                                                                                 <  SRR19973072.1310929/150‑1 (MQ=60)
                   GCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGG                                                                                                                                 <  SRR19973072.3639817/150‑1 (MQ=60)
                     GTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGG                                                                                                                               >  SRR19973072.3430897/1‑150 (MQ=60)
                        CGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTG                                                                                                                            <  SRR19973072.4421626/150‑1 (MQ=60)
                          CAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTTCCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGTCGATCCTCGCGGTTCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGT                                                                                                                          <  SRR19973072.444265/150‑1 (MQ=60)
                          CAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGT                                                                                                                          <  SRR19973072.444272/150‑1 (MQ=60)
                          CAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGT                                                                                                                          >  SRR19973072.2754631/1‑150 (MQ=60)
                          CAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAAAGGGGCTGGT                                                                                                                          >  SRR19973072.2754642/1‑150 (MQ=60)
                          CAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTGTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGT                                                                                                                          <  SRR19973072.444195/150‑1 (MQ=60)
                             GCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGC                                                                                                                       <  SRR19973072.2830042/150‑1 (MQ=60)
                                 GCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACA                                                                                                                   >  SRR19973072.3484442/1‑150 (MQ=60)
                                   CACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATT                                                                                                                 >  SRR19973072.974383/1‑150 (MQ=60)
                                    ACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTC                                                                                                                <  SRR19973072.3485908/150‑1 (MQ=60)
                                    ACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTC                                                                                                                >  SRR19973072.4105175/1‑150 (MQ=60)
                                    ACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTC                                                                                                                >  SRR19973072.1457164/1‑150 (MQ=60)
                                      GCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCAT                                                                                                              <  SRR19973072.1710161/150‑1 (MQ=60)
                                      GCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCAT                                                                                                              <  SRR19973072.1710137/150‑1 (MQ=60)
                                          TTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCT                                                                                                          >  SRR19973072.1685635/1‑150 (MQ=60)
                                            ATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTT                                                                                                        >  SRR19973072.3519953/1‑150 (MQ=60)
                                            ATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTT                                                                                                        >  SRR19973072.3519971/1‑150 (MQ=60)
                                            ATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTT                                                                                                        <  SRR19973072.3567643/150‑1 (MQ=60)
                                            ATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTT                                                                                                        >  SRR19973072.2562054/1‑150 (MQ=60)
                                            ATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAAACGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTT                                                                                                        >  SRR19973072.2562076/1‑150 (MQ=60)
                                             TTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAAACGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTTAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTG                                                                                                       >  SRR19973072.2579180/1‑150 (MQ=60)
                                                    GCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGT                                                                                                <  SRR19973072.4450787/150‑1 (MQ=60)
                                                    GCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGT                                                                                                <  SRR19973072.4450793/150‑1 (MQ=60)
                                                    GCAATACGCTGGCGA‑TTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTT                                                                                               >  SRR19973072.627798/1‑150 (MQ=60)
                                                     CAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTT                                                                                               <  SRR19973072.3936003/150‑1 (MQ=60)
                                                      AATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTA                                                                                              >  SRR19973072.2686066/1‑150 (MQ=60)
                                                      AATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAAACAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTA                                                                                              >  SRR19973072.4363011/1‑150 (MQ=60)
                                                       ATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTAC                                                                                             <  SRR19973072.1215854/150‑1 (MQ=60)
                                                        TACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGTGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACT                                                                                            <  SRR19973072.1656001/150‑1 (MQ=60)
                                                            CTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTG                                                                                        >  SRR19973072.469385/1‑150 (MQ=60)
                                                             TGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGT                                                                                       <  SRR19973072.2369701/150‑1 (MQ=60)
                                                             TGGCGATTTTTTTACCCTTAACCGGTGTGCNGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGT                                                                                       >  SRR19973072.4289463/1‑150 (MQ=60)
                                                              GGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTG                                                                                      <  SRR19973072.1890670/150‑1 (MQ=60)
                                                               GCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGG                                                                                     <  SRR19973072.3977266/150‑1 (MQ=60)
                                                                 GATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCT                                                                                   >  SRR19973072.807612/1‑150 (MQ=60)
                                                                 GATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCT                                                                                   >  SRR19973072.3501295/1‑150 (MQ=60)
                                                                 GATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCT                                                                                   >  SRR19973072.2615471/1‑150 (MQ=60)
                                                                 GATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCT                                                                                   >  SRR19973072.4463207/1‑150 (MQ=60)
                                                                 GATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCT                                                                                   <  SRR19973072.2264817/150‑1 (MQ=60)
                                                                   TTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGG                                                                                 <  SRR19973072.1136116/150‑1 (MQ=60)
                                                                   TTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGG                                                                                 <  SRR19973072.1136048/150‑1 (MQ=60)
                                                                   TTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGG                                                                                 <  SRR19973072.1766257/150‑1 (MQ=60)
                                                                       TTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATG                                                                             <  SRR19973072.2645375/150‑1 (MQ=60)
                                                                       TTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATG                                                                             >  SRR19973072.3151072/1‑150 (MQ=60)
                                                                          ACCCTTAACCGGTGTGCTGTGTGTGGTTCTACTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGG                                                                          >  SRR19973072.478081/1‑150 (MQ=60)
                                                                           CCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGG                                                                         >  SRR19973072.991017/1‑150 (MQ=60)
                                                                            CCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGC                                                                        >  SRR19973072.4040936/1‑150 (MQ=60)
                                                                             CTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCC                                                                       >  SRR19973072.1169122/1‑150 (MQ=60)
                                                                               TAACCGGTGTTCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCAC                                                                     >  SRR19973072.1323061/1‑150 (MQ=60)
                                                                                 ACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAG                                                                   >  SRR19973072.1177235/1‑150 (MQ=60)
                                                                                 ACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAG                                                                   >  SRR19973072.2251916/1‑150 (MQ=60)
                                                                                  CCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGC                                                                  >  SRR19973072.2348867/1‑150 (MQ=60)
                                                                                    GGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGT                                                                >  SRR19973072.2829820/1‑150 (MQ=60)
                                                                                     GTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTC                                                               <  SRR19973072.2654016/150‑1 (MQ=60)
                                                                                         GCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCA                                                           <  SRR19973072.1370832/150‑1 (MQ=60)
                                                                                         GCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCA                                                           <  SRR19973072.2685976/150‑1 (MQ=60)
                                                                                           TGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGT                                                         >  SRR19973072.4127771/1‑150 (MQ=60)
                                                                                            GTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTT                                                        <  SRR19973072.258177/150‑1 (MQ=60)
                                                                                              GTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGT                                                      <  SRR19973072.417852/150‑1 (MQ=60)
                                                                                              GTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGT                                                      >  SRR19973072.247517/1‑150 (MQ=60)
                                                                                                  GGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCT                                                  >  SRR19973072.1264575/1‑150 (MQ=60)
                                                                                                    TTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTAT                                                >  SRR19973072.669758/1‑150 (MQ=60)
                                                                                                    TTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTAT                                                <  SRR19973072.3156636/150‑1 (MQ=60)
                                                                                                       TGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGT                                             <  SRR19973072.2637849/150‑1 (MQ=60)
                                                                                                       TGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGT                                             >  SRR19973072.3315165/1‑150 (MQ=60)
                                                                                                       TGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGT                                             >  SRR19973072.3315174/1‑150 (MQ=60)
                                                                                                       TGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGT                                             <  SRR19973072.2637853/150‑1 (MQ=60)
                                                                                                       TGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGT                                             <  SRR19973072.4630542/150‑1 (MQ=60)
                                                                                                       TGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGT                                             <  SRR19973072.2638200/150‑1 (MQ=60)
                                                                                                        GCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTT                                            <  SRR19973072.1185052/150‑1 (MQ=60)
                                                                                                        GCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTT                                            <  SRR19973072.206891/150‑1 (MQ=60)
                                                                                                          TGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGC                                          >  SRR19973072.3521042/1‑150 (MQ=60)
                                                                                                           GGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCC                                         >  SRR19973072.1570784/1‑150 (MQ=60)
                                                                                                           GGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCC                                         >  SRR19973072.1570840/1‑150 (MQ=60)
                                                                                                             CGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCA                                       <  SRR19973072.1532892/150‑1 (MQ=60)
                                                                                                                 CCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTT                                   >  SRR19973072.4224297/1‑150 (MQ=60)
                                                                                                                  CTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACATTTGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTA                                  <  SRR19973072.1503800/150‑1 (MQ=60)
                                                                                                                  CTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTA                                  >  SRR19973072.1909522/1‑150 (MQ=60)
                                                                                                                  CTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTA                                  >  SRR19973072.1909519/1‑150 (MQ=60)
                                                                                                                     GCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTA                               >  SRR19973072.647289/1‑150 (MQ=60)
                                                                                                                     GCGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTA                               >  SRR19973072.647192/1‑150 (MQ=60)
                                                                                                                      CGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAC                              >  SRR19973072.525847/1‑150 (MQ=60)
                                                                                                                      CGGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTAC                              >  SRR19973072.1400315/1‑150 (MQ=60)
                                                                                                                       GGATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACC                             >  SRR19973072.3669205/1‑150 (MQ=60)
                                                                                                                        GATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCT                            <  SRR19973072.102612/150‑1 (MQ=60)
                                                                                                                        GATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCT                            <  SRR19973072.381612/150‑1 (MQ=60)
                                                                                                                         ATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTG                           >  SRR19973072.1435643/1‑150 (MQ=60)
                                                                                                                         ATCAGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTG                           >  SRR19973072.3367141/1‑150 (MQ=60)
                                                                                                                            AGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTC                        >  SRR19973072.1188020/1‑150 (MQ=60)
                                                                                                                            AGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTC                        >  SRR19973072.1188128/1‑150 (MQ=60)
                                                                                                                            AGTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTC                        <  SRR19973072.3654970/150‑1 (MQ=60)
                                                                                                                             GTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCT                       <  SRR19973072.2994167/150‑1 (MQ=60)
                                                                                                                             GTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCT                       <  SRR19973072.2018553/150‑1 (MQ=60)
                                                                                                                             GTCGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCT                       <  SRR19973072.1919565/150‑1 (MQ=60)
                                                                                                                               CGACGATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGC                     >  SRR19973072.267811/1‑150 (MQ=60)
                                                                                                                                   GATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACT                 >  SRR19973072.2474993/1‑150 (MQ=60)
                                                                                                                                   GATCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACT                 <  SRR19973072.2670996/150‑1 (MQ=60)
                                                                                                                                     TCAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGG               <  SRR19973072.2101318/150‑1 (MQ=60)
                                                                                                                                      CAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGT              >  SRR19973072.834319/1‑150 (MQ=60)
                                                                                                                                      CAATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGT              <  SRR19973072.4494281/150‑1 (MQ=60)
                                                                                                                                       AATAGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTG             <  SRR19973072.3945061/150‑1 (MQ=60)
                                                                                                                                          AGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCG          >  SRR19973072.3053293/1‑150 (MQ=60)
                                                                                                                                          AGTGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCG          >  SRR19973072.3053292/1‑150 (MQ=60)
                                                                                                                                            TGAAGCGCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGG        >  SRR19973072.3803915/1‑150 (MQ=60)
                                                                                                                                                  GCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTA  <  SRR19973072.4182240/150‑1 (MQ=60)
                                                                                                                                                  GCTAATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTA  <  SRR19973072.3373239/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
ACTATTCCCCGCGTAGATGGCGTTCGCAAGCCTGCCACGCCTTTATTGCCGGGCAATACGCTGGCGATTTTTTTACCCTTAACCGGTGTGCTGTGTGTGGTTCTGCTGGCGATCCTCGCGGATCAGTCGACGATCAATAGTGAAGCGCTCATCAACAGCCTGACAATGGGGCTGGTGGCGACATTCATCGCTTTGCTCCTGTTACTGCTGTGGCTGGAATGGGGGCCACAGCGTCGCCAGTTGTGGCTATGGTTGCCCATTTTATTACCTGCTCTGCCACTGGTGGCGGGCCAGTA  >  CP009273/1832264‑1832559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑