Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,849,550 C→A 13.3% G227C (GGC→TGC)  ydjG ← alpha‑Keto reductase, NADH‑dependent; can use methylglyoxal as substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,849,5500CA13.3% 985.2 / 81.9 284G227C (GGC→TGC) ydjGalpha‑Keto reductase, NADH‑dependent; can use methylglyoxal as substrate
Reads supporting (aligned to +/- strand):  ref base C (119/127);  new base A (10/28);  total (129/155)
Fisher's exact test for biased strand distribution p-value = 1.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

AGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGC  >  CP009273/1849402‑1849699
                                                                                                                                                    |                                                                                                                                                     
aGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCNCCCGGAACGTAATCACGAGTGATGGTGCCg                                                                                                                                                      <  1:4283755/150‑1 (MQ=255)
aGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCg                                                                                                                                                      <  1:514664/150‑1 (MQ=255)
aGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCg                                                                                                                                                      <  1:3341694/150‑1 (MQ=255)
    ccAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTca                                                                                                                                                  >  1:640972/1‑150 (MQ=255)
      agagTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTcaac                                                                                                                                                >  1:4175107/1‑150 (MQ=255)
        agTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTcaacaa                                                                                                                                              >  2:3554119/1‑150 (MQ=255)
         gTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAAt                                                                                                                                             <  1:622568/150‑1 (MQ=255)
          tGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATc                                                                                                                                            >  1:4341357/1‑150 (MQ=255)
            ggAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATccc                                                                                                                                          >  2:1102757/1‑150 (MQ=255)
             gAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCt                                                                                                                                         <  1:4416892/150‑1 (MQ=255)
             gAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCt                                                                                                                                         <  1:1978894/150‑1 (MQ=255)
              aaTTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTg                                                                                                                                        <  2:4293010/150‑1 (MQ=255)
              aaTTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTg                                                                                                                                        <  2:2590161/150‑1 (MQ=255)
                ttGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGct                                                                                                                                      <  1:125676/150‑1 (MQ=255)
                   tgCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTa                                                                                                                                   <  1:2258826/150‑1 (MQ=255)
                    gCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAg                                                                                                                                  <  1:985083/150‑1 (MQ=255)
                    gCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACGTTATTTGCCCGAGCGCCGCCCGGAACGAACTCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCGAg                                                                                                                                  >  1:1650604/1‑150 (MQ=255)
                     cACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGc                                                                                                                                 >  1:323087/1‑150 (MQ=255)
                      aCTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCg                                                                                                                                >  2:1540653/1‑150 (MQ=255)
                       cTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCgg                                                                                                                               >  2:3458827/1‑150 (MQ=255)
                           tAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGaa                                                                                                                           <  2:179388/150‑1 (MQ=255)
                           tAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGaa                                                                                                                           <  1:3588363/150‑1 (MQ=255)
                            aaCGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAAt                                                                                                                          <  1:938670/150‑1 (MQ=255)
                              cGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATaa                                                                                                                        <  1:2629950/150‑1 (MQ=255)
                                aGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAAc                                                                                                                      <  2:1325488/150‑1 (MQ=255)
                                    caAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGa                                                                                                                  >  2:2051818/1‑150 (MQ=255)
                                    caAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGa                                                                                                                  >  2:492752/1‑150 (MQ=255)
                                     aaaGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGaa                                                                                                                 >  2:4587330/1‑150 (MQ=255)
                                      aaGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAAc                                                                                                                >  2:3288474/1‑150 (MQ=255)
                                          ggCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACcaca                                                                                                            >  2:4492460/1‑150 (MQ=255)
                                          ggCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACcaca                                                                                                            >  2:2160981/1‑150 (MQ=255)
                                                cACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCa                                                                                                      >  2:2509529/1‑150 (MQ=255)
                                                cACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCa                                                                                                      >  2:1408788/1‑150 (MQ=255)
                                                  cTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACACTGCCAtt                                                                                                    <  2:2261465/150‑1 (MQ=255)
                                                  cTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCAtt                                                                                                    >  1:2895798/1‑150 (MQ=255)
                                                    gTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTAt                                                                                                  <  2:874465/150‑1 (MQ=255)
                                                    gTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTAt                                                                                                  >  1:393539/1‑150 (MQ=255)
                                                    gTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTAt                                                                                                  >  1:2924935/1‑150 (MQ=255)
                                                       cAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCAc                                                                                               >  2:510743/1‑150 (MQ=255)
                                                        aaGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACg                                                                                              >  2:4002040/1‑150 (MQ=255)
                                                          gCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGAc                                                                                            <  2:2358470/150‑1 (MQ=255)
                                                              atcaatcaCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAg                                                                                        >  2:3362260/1‑150 (MQ=255)
                                                              atcaatcaCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAg                                                                                        <  2:3641697/150‑1 (MQ=255)
                                                                    caCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGgcag                                                                                  >  1:2763417/1‑150 (MQ=255)
                                                                     aCTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGgcagc                                                                                 <  1:115891/150‑1 (MQ=255)
                                                                           aGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCg                                                                           >  1:143623/1‑150 (MQ=255)
                                                                           aGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCg                                                                           <  2:3742166/150‑1 (MQ=255)
                                                                            gCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGt                                                                          <  1:4553311/150‑1 (MQ=255)
                                                                             cATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtt                                                                         <  1:2193670/150‑1 (MQ=255)
                                                                             cATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtt                                                                         <  1:2648768/150‑1 (MQ=255)
                                                                              aTGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACCTAGTGGCAGCAGTTCGttt                                                                        >  2:3212814/1‑150 (MQ=255)
                                                                               tGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtttt                                                                       >  1:848266/1‑150 (MQ=255)
                                                                               tGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGtttt                                                                       >  2:4121426/1‑150 (MQ=255)
                                                                                 ttttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTcc                                                                     <  2:3989555/150‑1 (MQ=255)
                                                                                  tttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCa                                                                    <  2:1700974/150‑1 (MQ=255)
                                                                                   ttCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCAt                                                                   <  1:1013681/150‑1 (MQ=255)
                                                                                    tCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCAtt                                                                  <  2:3822915/150‑1 (MQ=255)
                                                                                        gCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGACCGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGccc                                                              >  2:3944384/1‑150 (MQ=255)
                                                                                           ggAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGt                                                           <  1:2997150/150‑1 (MQ=255)
                                                                                              aCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGa                                                        <  2:3498546/150‑1 (MQ=255)
                                                                                              aCCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGa                                                        >  2:748442/1‑150 (MQ=255)
                                                                                               ccAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAg                                                       <  1:2462946/150‑1 (MQ=255)
                                                                                                cAGACTTTTTTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAgg                                                      <  1:3782604/150‑1 (MQ=255)
                                                                                                cAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACACTCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAgg                                                      <  2:3783463/150‑1 (MQ=255)
                                                                                                 aGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGa                                                     >  2:4312056/1‑150 (MQ=255)
                                                                                                  gACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTTTCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGAt                                                    <  1:768668/150‑1 (MQ=255)
                                                                                                  gACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGAt                                                    >  1:2431632/1‑150 (MQ=255)
                                                                                                   aCTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGCTa                                                   >  2:4516488/1‑150 (MQ=255)
                                                                                                     tttATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACt                                                 <  1:1190908/150‑1 (MQ=255)
                                                                                                        aTTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTa                                              >  1:2366009/1‑150 (MQ=255)
                                                                                                        aTTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTa                                              <  2:3772032/150‑1 (MQ=255)
                                                                                                           tGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTAttt                                           <  1:4001928/150‑1 (MQ=255)
                                                                                                           tGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTAttt                                           <  1:3433025/150‑1 (MQ=255)
                                                                                                            gCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTc                                          <  2:3341673/150‑1 (MQ=255)
                                                                                                                gAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCt                                      <  1:985278/150‑1 (MQ=255)
                                                                                                                 aGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTg                                     <  2:2823944/150‑1 (MQ=255)
                                                                                                                    gccgccCGGAACGTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGaat                                  <  1:4006564/150‑1 (MQ=255)
                                                                                                                      cgccCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGaataa                                >  2:3512098/1‑150 (MQ=255)
                                                                                                                         ccGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAatat                             >  2:305202/1‑150 (MQ=255)
                                                                                                                         ccGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAatat                             <  1:1945877/150‑1 (MQ=255)
                                                                                                                          cGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATc                            <  1:3288474/150‑1 (MQ=255)
                                                                                                                          cGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATc                            >  1:4456096/1‑150 (MQ=255)
                                                                                                                            gAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCa                          <  2:1199892/150‑1 (MQ=255)
                                                                                                                            gAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCa                          <  1:3387696/150‑1 (MQ=255)
                                                                                                                             aaCGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAg                         >  2:3961458/1‑150 (MQ=255)
                                                                                                                             aaCGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAg                         <  2:243576/150‑1 (MQ=255)
                                                                                                                                gTAATCACGAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTc                      <  1:1101020/150‑1 (MQ=255)
                                                                                                                                 tAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCa                     <  2:2487080/150‑1 (MQ=255)
                                                                                                                                  aaTCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCAc                    >  2:3137931/1‑150 (MQ=255)
                                                                                                                                   aTCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCAcc                   <  1:2513956/150‑1 (MQ=255)
                                                                                                                                      aCGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCata                >  2:77232/1‑150 (MQ=255)
                                                                                                                                        gAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATAtt              <  2:19755/150‑1 (MQ=255)
                                                                                                                                        gAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATAtt              <  2:19643/150‑1 (MQ=255)
                                                                                                                                        gAGTGATGGTGCAGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATAtt              <  1:2038982/150‑1 (MQ=255)
                                                                                                                                           tGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCa           >  1:131427/1‑150 (MQ=255)
                                                                                                                                            gATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAg          <  2:648724/150‑1 (MQ=255)
                                                                                                                                                 tGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACt     <  1:1254335/150‑1 (MQ=255)
                                                                                                                                                  gCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTc    >  2:2650565/1‑150 (MQ=255)
                                                                                                                                                  gCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTc    <  2:3789699/150‑1 (MQ=255)
                                                                                                                                                   ccGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTcg   >  1:2546964/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTGCCAGAGTGGGAATTGTGCACTGATAACGAGCACAAAGTGGCTGCCACTGTTCAAGCATATCAATCACTTTCAGCATGTTTTCACGCTGGAACCAGACTTTATTTGCCCGAGCGCCGCCCGGAACGTAATCACGAGTGATGGTGCCGGTCAACAATCCCTGCTCTAGCGGGGAATAAACCTGAACCACAATGCCATTATCACGACATAGTGGCAGCAGTTCGTTTTCCATTGCCCGGTCGAGGATACTGTATTTCGCCTGAATAATATCCAGTTCACCATATTGCAGATACTCGC  >  CP009273/1849402‑1849699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A