Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,865,422 C→A 94.1% R261R (CGA→AGA)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,865,4220CA94.1% 755.3 / 35.3 237R261R (CGA→AGA) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (3/11);  new base A (91/132);  total (94/143)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 237 total aligned reads displayed.

CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTT  >  CP009273/1865275‑1865565
                                                                                                                                                   |                                                                                                                                               
 acaATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGAt                                                                                                                                              >  1:1388351/1‑150 (MQ=255)
   aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                            <  2:4743692/150‑1 (MQ=255)
   aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                            >  1:4308106/1‑150 (MQ=255)
    aTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAg                                                                                                                                           >  2:1979043/1‑150 (MQ=255)
    aTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAg                                                                                                                                           >  2:1979030/1‑150 (MQ=255)
     tCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGc                                                                                                                                          <  2:1662880/150‑1 (MQ=255)
         aGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTTTTTATTATCATGCGCACAAGATTAGCCAGc                                                                                                                                      <  1:1035376/150‑1 (MQ=255)
         aGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGc                                                                                                                                      <  1:1374684/150‑1 (MQ=255)
         aGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGc                                                                                                                                      <  1:2497182/150‑1 (MQ=255)
            aGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGACTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGaa                                                                                                                                   >  2:4131109/1‑150 (MQ=255)
             gTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAAc                                                                                                                                  >  2:4191238/1‑150 (MQ=255)
             gTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAAc                                                                                                                                  >  2:4573290/1‑150 (MQ=255)
                ataACGCTGACTAGTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTAt                                                                                                                               <  2:3729547/150‑1 (MQ=255)
                  aaCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATgg                                                                                                                             <  1:4464834/150‑1 (MQ=255)
                   aCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGc                                                                                                                            <  2:3579921/150‑1 (MQ=255)
                    cGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGcc                                                                                                                           <  1:973045/150‑1 (MQ=255)
                    cGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGcc                                                                                                                           >  2:4243494/1‑150 (MQ=255)
                    cGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGcc                                                                                                                           >  2:820995/1‑150 (MQ=255)
                      cTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGt                                                                                                                         <  2:1845896/150‑1 (MQ=255)
                       tGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGtt                                                                                                                        <  1:4663959/150‑1 (MQ=255)
                       tGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGtt                                                                                                                        <  1:3157500/150‑1 (MQ=255)
                       tGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGtt                                                                                                                        <  1:571281/150‑1 (MQ=255)
                         aCTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTaa                                                                                                                      <  1:4199538/150‑1 (MQ=255)
                          cTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAAt                                                                                                                     <  2:2879510/150‑1 (MQ=255)
                          cTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTAGTATCATGCGCACAAGATTAGCCCGCGACCTATGTCCGTTaaa                                                                                                                     >  2:307702/1‑149 (MQ=255)
                           tATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATa                                                                                                                    <  2:4866285/150‑1 (MQ=255)
                              tCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCa                                                                                                                 >  2:2627826/1‑150 (MQ=255)
                               cTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCat                                                                                                                <  2:3607221/150‑1 (MQ=255)
                                    tATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCAttatta                                                                                                           >  2:2518612/1‑150 (MQ=255)
                                     aTGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATtattat                                                                                                          >  2:703972/1‑150 (MQ=255)
                                      tGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATg                                                                                                         <  2:5094618/150‑1 (MQ=255)
                                         cgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCt                                                                                                      >  1:403187/1‑150 (MQ=255)
                                          gtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTg                                                                                                     >  1:2575142/1‑150 (MQ=255)
                                          gtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTg                                                                                                     >  1:1869002/1‑150 (MQ=255)
                                          gtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTg                                                                                                     <  2:1057627/150‑1 (MQ=255)
                                          gtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTg                                                                                                     <  1:1110250/150‑1 (MQ=255)
                                           tcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTgg                                                                                                    >  2:1771631/1‑150 (MQ=255)
                                            cgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGc                                                                                                   <  2:1512586/150‑1 (MQ=255)
                                            cgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGc                                                                                                   <  1:2105685/150‑1 (MQ=255)
                                              aTTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCAt                                                                                                 >  1:4975592/1‑150 (MQ=255)
                                                  cTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCt                                                                                             <  1:5055255/150‑1 (MQ=255)
                                                  cTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCt                                                                                             <  2:2322771/150‑1 (MQ=255)
                                                   tGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTa                                                                                            <  2:1866566/150‑1 (MQ=255)
                                                     tCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAtg                                                                                          >  2:966149/1‑150 (MQ=255)
                                                      ccGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAtgc                                                                                         >  1:148202/1‑150 (MQ=255)
                                                           cAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCaa                                                                                    >  2:1509676/1‑150 (MQ=255)
                                                           cAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCaa                                                                                    >  2:3794086/1‑150 (MQ=255)
                                                            aaCCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAac                                                                                   >  1:355592/1‑150 (MQ=255)
                                                               cATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAactta                                                                                <  2:4271002/150‑1 (MQ=255)
                                                                  aCTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAacttactt                                                                             >  2:3981083/1‑150 (MQ=255)
                                                                    tGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCt                                                                           <  1:1429824/150‑1 (MQ=255)
                                                                      cGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                         <  1:1629636/150‑1 (MQ=255)
                                                                       gACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACt                                                                        >  1:706270/1‑150 (MQ=255)
                                                                            ggggAATCAACTATACAAAAATATTGGTTTGTCTGTGTGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGActc                                                                   <  2:2968840/150‑1 (MQ=255)
                                                                               gAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATGTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTgg                                                                <  1:5122077/150‑1 (MQ=255)
                                                                                aaTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGa                                                               >  1:481853/1‑150 (MQ=255)
                                                                                 aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAt                                                              <  1:4112666/150‑1 (MQ=255)
                                                                                 aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAt                                                              <  2:4853396/150‑1 (MQ=255)
                                                                                   cAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGa                                                            <  2:232049/150‑1 (MQ=255)
                                                                                    aaCTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAg                                                           >  1:1495439/1‑150 (MQ=255)
                                                                                       tataCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGtat                                                        <  2:371330/150‑1 (MQ=255)
                                                                                        ataCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGtata                                                       <  2:2464236/150‑1 (MQ=255)
                                                                                         taCAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATaa                                                      >  2:2010598/1‑150 (MQ=255)
                                                                                           cAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAAtt                                                    <  1:906504/150‑1 (MQ=255)
                                                                                           cAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAAtt                                                    >  1:3672997/1‑150 (MQ=255)
                                                                                              aaaTATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATtata                                                 <  2:3851683/150‑1 (MQ=255)
                                                                                                    tGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCt                                           >  2:1081977/1‑150 (MQ=255)
                                                                                                    tGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCt                                           <  1:1418366/150‑1 (MQ=255)
                                                                                                      gTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTgg                                         <  2:3257428/150‑1 (MQ=255)
                                                                                                        ttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                       <  1:840845/150‑1 (MQ=255)
                                                                                                        ttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                       <  1:2462468/150‑1 (MQ=255)
                                                                                                        ttGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                       <  2:2605481/150‑1 (MQ=255)
                                                                                                         tGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtat                                      >  2:4492925/1‑150 (MQ=255)
                                                                                                          gTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtata                                     >  1:2227648/1‑150 (MQ=255)
                                                                                                             tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCa                                  >  2:4659273/1‑150 (MQ=255)
                                                                                                             tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCa                                  >  1:3418163/1‑150 (MQ=255)
                                                                                                             tgtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCa                                  <  1:2899031/150‑1 (MQ=255)
                                                                                                              gtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAg                                 >  2:772691/1‑150 (MQ=255)
                                                                                                              gtgGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACGTACTTCTCCTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAg                                 <  1:5132468/150‑1 (MQ=255)
                                                                                                                gggCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTc                               <  2:2168976/150‑1 (MQ=255)
                                                                                                                gggCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTc                               <  2:156273/150‑1 (MQ=255)
                                                                                                                 ggCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcg                              >  1:2522868/1‑150 (MQ=255)
                                                                                                                  gCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcgc                             <  1:3352885/150‑1 (MQ=255)
                                                                                                                     tttttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCggg                          >  1:3123359/1‑150 (MQ=255)
                                                                                                                        ttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAtt                       <  2:3457675/150‑1 (MQ=255)
                                                                                                                        ttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAtt                       <  2:3179923/150‑1 (MQ=255)
                                                                                                                         tACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTg                      >  1:3923229/1‑150 (MQ=255)
                                                                                                                          aCTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGa                     <  1:2825975/150‑1 (MQ=255)
                                                                                                                          aCTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGa                     >  1:1294019/1‑150 (MQ=255)
                                                                                                                            tGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAg                   <  1:4243494/150‑1 (MQ=255)
                                                                                                                               tCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGttt                <  2:564508/150‑1 (MQ=255)
                                                                                                                                cTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTc               >  2:3342974/1‑150 (MQ=255)
                                                                                                                                    attatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGt           <  2:2405143/150‑1 (MQ=255)
                                                                                                                                      tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTCTAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa         >  2:1658573/1‑150 (MQ=255)
                                                                                                                                      tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa         >  2:1469808/1‑150 (MQ=255)
                                                                                                                                       atCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaaa        >  1:3012391/1‑150 (MQ=255)
                                                                                                                                        tCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAAc       >  2:2767188/1‑150 (MQ=255)
                                                                                                                                         cATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACt      <  1:4131109/150‑1 (MQ=255)
                                                                                                                                            gcgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtt   >  1:4566003/1‑150 (MQ=255)
                                                                                                                                             cgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgttt  <  2:3946874/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTT  >  CP009273/1865275‑1865565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTG  >  CP009273/1865275‑1865571
                                                                                                                                                   |                                                                                                                                                     
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGA                                                                                                                                                     >  SRR19973077.2172721/1‑150 (MQ=60)
 ACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGAT                                                                                                                                                    >  SRR19973077.1390782/1‑150 (MQ=60)
   AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTA                                                                                                                                                  >  SRR19973077.4315668/1‑150 (MQ=60)
   AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTA                                                                                                                                                  >  SRR19973077.4231377/1‑150 (MQ=60)
       TGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCA                                                                                                                                              >  SRR19973077.2480611/1‑150 (MQ=60)
        GAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAG                                                                                                                                             >  SRR19973077.1495135/1‑150 (MQ=60)
         AGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTTTTTATTATCATGCGCACAAGATTAGCCAGC                                                                                                                                            <  SRR19973077.1037206/150‑1 (MQ=60)
         AGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGC                                                                                                                                            <  SRR19973077.1377646/150‑1 (MQ=60)
         AGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGC                                                                                                                                            <  SRR19973077.2501600/150‑1 (MQ=60)
         AGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGC                                                                                                                                            <  SRR19973077.1377097/150‑1 (MQ=60)
          GCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCG                                                                                                                                           >  SRR19973077.4813095/1‑150 (MQ=60)
            AGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAA                                                                                                                                         <  SRR19973077.2388652/150‑1 (MQ=60)
                ATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTAT                                                                                                                                     <  SRR19973077.1582430/150‑1 (MQ=60)
                  AACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATCCTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGG                                                                                                                                   <  SRR19973077.4472749/150‑1 (MQ=60)
                  AACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGG                                                                                                                                   <  SRR19973077.4472740/150‑1 (MQ=60)
                   ACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGC                                                                                                                                  <  SRR19973077.5055777/150‑1 (MQ=60)
                    CGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCC                                                                                                                                 <  SRR19973077.974765/150‑1 (MQ=60)
                    CGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCC                                                                                                                                 <  SRR19973077.1920655/150‑1 (MQ=60)
                       TGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTT                                                                                                                              <  SRR19973077.3163103/150‑1 (MQ=60)
                       TGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTT                                                                                                                              <  SRR19973077.4672166/150‑1 (MQ=60)
                       TGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTT                                                                                                                              <  SRR19973077.572306/150‑1 (MQ=60)
                        GACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTA                                                                                                                             <  SRR19973077.4095733/150‑1 (MQ=60)
                         ACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAA                                                                                                                            <  SRR19973077.4206927/150‑1 (MQ=60)
                          CTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAAT                                                                                                                           <  SRR19973077.3113445/150‑1 (MQ=60)
                              TCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCA                                                                                                                       >  SRR19973077.4138079/1‑150 (MQ=60)
                                   GTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATT                                                                                                                  <  SRR19973077.144703/150‑1 (MQ=60)
                                      TGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATG                                                                                                               >  SRR19973077.1575449/1‑150 (MQ=60)
                                         CGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCT                                                                                                            >  SRR19973077.403870/1‑150 (MQ=60)
                                          GTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTG                                                                                                           >  SRR19973077.882890/1‑150 (MQ=60)
                                          GTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTG                                                                                                           >  SRR19973077.2579694/1‑150 (MQ=60)
                                          GTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTG                                                                                                           <  SRR19973077.2751827/150‑1 (MQ=60)
                                          GTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTG                                                                                                           <  SRR19973077.1112192/150‑1 (MQ=60)
                                          GTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTG                                                                                                           >  SRR19973077.1872278/1‑150 (MQ=60)
                                            CGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGC                                                                                                         <  SRR19973077.2109333/150‑1 (MQ=60)
                                            CGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGC                                                                                                         <  SRR19973077.2109302/150‑1 (MQ=60)
                                              ATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCAT                                                                                                       >  SRR19973077.4984438/1‑150 (MQ=60)
                                                  CTGTCCGGACAACCCTACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCT                                                                                                   <  SRR19973077.671213/150‑1 (MQ=60)
                                                  CTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCT                                                                                                   <  SRR19973077.5064221/150‑1 (MQ=60)
                                                  CTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCT                                                                                                   <  SRR19973077.5064227/150‑1 (MQ=60)
                                                  CTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCT                                                                                                   <  SRR19973077.3253983/150‑1 (MQ=60)
                                                      CCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGC                                                                                               >  SRR19973077.148491/1‑150 (MQ=60)
                                                            AACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAAC                                                                                         >  SRR19973077.356215/1‑150 (MQ=60)
                                                                 TACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACT                                                                                    <  SRR19973077.3354630/150‑1 (MQ=60)
                                                                    TGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCT                                                                                 <  SRR19973077.1432321/150‑1 (MQ=60)
                                                                    TGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCT                                                                                 <  SRR19973077.1432208/150‑1 (MQ=60)
                                                                    TGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCT                                                                                 <  SRR19973077.1432179/150‑1 (MQ=60)
                                                                      CGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAC                                                                               <  SRR19973077.740534/150‑1 (MQ=60)
                                                                      CGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAC                                                                               <  SRR19973077.1632463/150‑1 (MQ=60)
                                                                      CGACCTGGGGAATCAACTAGACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAC                                                                               <  SRR19973077.3967217/150‑1 (MQ=60)
                                                                       GACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACT                                                                              >  SRR19973077.707517/1‑150 (MQ=60)
                                                                       GACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACT                                                                              >  SRR19973077.4886892/1‑150 (MQ=60)
                                                                         CCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGA                                                                            >  SRR19973077.1806727/1‑150 (MQ=60)
                                                                              GGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTG                                                                       <  SRR19973077.4656918/150‑1 (MQ=60)
                                                                               GAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGG                                                                      <  SRR19973077.2853011/150‑1 (MQ=60)
                                                                               GAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGG                                                                      <  SRR19973077.5131193/150‑1 (MQ=60)
                                                                               GAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATGTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGG                                                                      <  SRR19973077.5131146/150‑1 (MQ=60)
                                                                                AATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGA                                                                     >  SRR19973077.482702/1‑150 (MQ=60)
                                                                                 ATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAT                                                                    <  SRR19973077.4119915/150‑1 (MQ=60)
                                                                                 ATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAT                                                                    <  SRR19973077.4119788/150‑1 (MQ=60)
                                                                                  TCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATG                                                                   <  SRR19973077.2571317/150‑1 (MQ=60)
                                                                                   CAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGA                                                                  <  SRR19973077.5150245/150‑1 (MQ=60)
                                                                                    AACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAG                                                                 >  SRR19973077.1498049/1‑150 (MQ=60)
                                                                                           CAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATT                                                          >  SRR19973077.3679474/1‑150 (MQ=60)
                                                                                           CAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATT                                                          <  SRR19973077.908117/150‑1 (MQ=60)
                                                                                           CAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATT                                                          <  SRR19973077.127696/150‑1 (MQ=60)
                                                                                                ATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACT                                                     <  SRR19973077.945647/150‑1 (MQ=60)
                                                                                                 TATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTA                                                    <  SRR19973077.5057712/150‑1 (MQ=60)
                                                                                                    TGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCT                                                 <  SRR19973077.1420841/150‑1 (MQ=60)
                                                                                                     GGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTG                                                <  SRR19973077.3584165/150‑1 (MQ=60)
                                                                                                        TTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTA                                             <  SRR19973077.842319/150‑1 (MQ=60)
                                                                                                        TTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTA                                             <  SRR19973077.2466823/150‑1 (MQ=60)
                                                                                                        TTGTCTGTGGGCATTTTTACTGTTCTTGATTCTCATGCGCTCAAGATTAGCCAGCGATCTATGGCCGTTAATAGCATTATTATGTCTGGCATCGATATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTA                                             <  SRR19973077.842339/150‑1 (MQ=60)
                                                                                                         TGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTAT                                            >  SRR19973077.3617707/1‑150 (MQ=60)
                                                                                                          GTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATA                                           <  SRR19973077.991344/150‑1 (MQ=60)
                                                                                                          GTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATA                                           >  SRR19973077.2231547/1‑150 (MQ=60)
                                                                                                          GTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATA                                           >  SRR19973077.4403685/1‑150 (MQ=60)
                                                                                                          GTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATA                                           >  SRR19973077.4403703/1‑150 (MQ=60)
                                                                                                             TGTGGGCATTTTTACTTTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTATGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGTATGATTATAATTATACTATCTGGTATATCA                                        <  SRR19973077.2960292/150‑1 (MQ=60)
                                                                                                             TGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCA                                        <  SRR19973077.2904156/150‑1 (MQ=60)
                                                                                                             TGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCA                                        >  SRR19973077.3424169/1‑150 (MQ=60)
                                                                                                              GTGGTCATTTTTACTGTTCTTTATTATCATGCTCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTTTTATGTCTGGCATATCTATGATGAAACTTACTTCTACTGACTATGGATGAGTATAATTATACTATCTGGTATATCAG                                       <  SRR19973077.13711/150‑1 (MQ=60)
                                                                                                              GTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAG                                       <  SRR19973077.14113/150‑1 (MQ=60)
                                                                                                              GTGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACGTACTTCTCCTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAG                                       <  SRR19973077.5141554/150‑1 (MQ=60)
                                                                                                               TGGGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCCGGTATATCAGT                                      <  SRR19973077.1083623/150‑1 (MQ=60)
                                                                                                                 GGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCG                                    >  SRR19973077.2527329/1‑150 (MQ=60)
                                                                                                                 GGCATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCG                                    >  SRR19973077.2527337/1‑150 (MQ=60)
                                                                                                                  GCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGC                                   <  SRR19973077.3359067/150‑1 (MQ=60)
                                                                                                                  GCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGC                                   <  SRR19973077.3358776/150‑1 (MQ=60)
                                                                                                                    ATTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGG                                 <  SRR19973077.4712839/150‑1 (MQ=60)
                                                                                                                     TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGG                                >  SRR19973077.3128892/1‑150 (MQ=60)
                                                                                                                     TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGG                                >  SRR19973077.3128893/1‑150 (MQ=60)
                                                                                                                      TTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGA                               <  SRR19973077.4266153/150‑1 (MQ=60)
                                                                                                                        TTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATT                             >  SRR19973077.4139934/1‑150 (MQ=60)
                                                                                                                         TACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTG                            >  SRR19973077.3930153/1‑150 (MQ=60)
                                                                                                                         TACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTG                            >  SRR19973077.3930139/1‑150 (MQ=60)
                                                                                                                          ACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGA                           >  SRR19973077.1296274/1‑150 (MQ=60)
                                                                                                                          ACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGA                           >  SRR19973077.1296328/1‑150 (MQ=60)
                                                                                                                          ACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGA                           <  SRR19973077.2831004/150‑1 (MQ=60)
                                                                                                                            TGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAG                         <  SRR19973077.4250951/150‑1 (MQ=60)
                                                                                                                                 TTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCC                    <  SRR19973077.979800/150‑1 (MQ=60)
                                                                                                                                   TATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAG                  >  SRR19973077.560994/1‑150 (MQ=60)
                                                                                                                                       ATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAA              >  SRR19973077.418368/1‑150 (MQ=60)
                                                                                                                                       ATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAA              >  SRR19973077.3017698/1‑150 (MQ=60)
                                                                                                                                        TCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAAC             <  SRR19973077.24939/150‑1 (MQ=60)
                                                                                                                                        TCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAAC             <  SRR19973077.2632488/150‑1 (MQ=60)
                                                                                                                                         CATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACT            <  SRR19973077.4138390/150‑1 (MQ=60)
                                                                                                                                            GCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTT         >  SRR19973077.4574055/1‑150 (MQ=60)
                                                                                                                                             CGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTCCTGACTCTGGATGAGTATAATTATACTATCTGGTATATCATTCGCGGGATTGAAGTTTCCAGTAAACTGTTT        <  SRR19973077.2951874/150‑1 (MQ=60)
                                                                                                                                             CGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTT        <  SRR19973077.346882/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    >  SRR19973077.5048163/1‑150 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    <  SRR19973077.2618480/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    <  SRR19973077.3207703/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    <  SRR19973077.3207731/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    <  SRR19973077.967858/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    <  SRR19973077.4587427/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    <  SRR19973077.3207821/150‑1 (MQ=60)
                                                                                                                                                 CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG    >  SRR19973077.3281813/1‑150 (MQ=60)
                                                                                                                                                  AAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGT   <  SRR19973077.5062965/150‑1 (MQ=60)
                                                                                                                                                   AGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTG  <  SRR19973077.3395044/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCATTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTG  >  CP009273/1865275‑1865571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑