Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,690,981 G→T 5.1% A110E (GCG→GAG)  uidR ← transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,690,9810GT 5.1% 1001.4 / 19.7 263A110E (GCG→GAG) uidRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (138/111);  new base T (9/5);  total (147/116)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GCAGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690832‑1691130
                                                                                                                                                     |                                                                                                                                                     
gcagTAAACGTGAAGTCATTGCCGTGCTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCg                                                                                                                                                       <  2:536326/150‑1 (MQ=255)
  agTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGcc                                                                                                                                                     >  1:408581/1‑150 (MQ=255)
   gTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCa                                                                                                                                                    >  2:406371/1‑150 (MQ=255)
       aCGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAt                                                                                                                                                <  2:2119157/150‑1 (MQ=255)
        cGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAtt                                                                                                                                               <  2:1170030/150‑1 (MQ=255)
         gTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAttt                                                                                                                                              >  2:803004/1‑150 (MQ=255)
         gTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAttt                                                                                                                                              >  2:1428667/1‑150 (MQ=255)
         gTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAAttt                                                                                                                                              >  2:3083258/1‑150 (MQ=255)
           gAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCa                                                                                                                                            <  1:951642/150‑1 (MQ=255)
            aaGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCaa                                                                                                                                           >  1:1482998/1‑150 (MQ=255)
                cATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAaccac                                                                                                                                       >  1:3863789/1‑150 (MQ=255)
                cATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAaccac                                                                                                                                       >  1:4542725/1‑150 (MQ=255)
                   tGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAg                                                                                                                                    <  1:3467751/150‑1 (MQ=255)
                      cGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAgcgc                                                                                                                                 >  1:2502784/1‑150 (MQ=255)
                       gtgtTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGcc                                                                                                                                <  1:583465/150‑1 (MQ=255)
                           tGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGtt                                                                                                                            <  2:4079557/150‑1 (MQ=255)
                                  tGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCaaa                                                                                                                     >  2:1658912/1‑150 (MQ=255)
                                  tGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCaaa                                                                                                                     >  1:1651746/1‑150 (MQ=255)
                                  tGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCaaa                                                                                                                     >  1:1403093/1‑150 (MQ=255)
                                     gCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGc                                                                                                                  <  1:2910534/150‑1 (MQ=255)
                                     gCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGc                                                                                                                  <  1:1804961/150‑1 (MQ=255)
                                        tATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTc                                                                                                               >  2:2992971/1‑150 (MQ=255)
                                         aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              <  2:605156/150‑1 (MQ=255)
                                         aTCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCa                                                                                                              <  2:2239580/150‑1 (MQ=255)
                                          tctcGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCAt                                                                                                             <  2:2919695/150‑1 (MQ=255)
                                              gCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGt                                                                                                         >  1:2786345/1‑150 (MQ=255)
                                               ccTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTg                                                                                                        <  2:1119760/150‑1 (MQ=255)
                                                 tttttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgag                                                                                                      >  2:3029912/1‑150 (MQ=255)
                                                 tttttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgag                                                                                                      <  1:4489135/150‑1 (MQ=255)
                                                  ttttGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTgaga                                                                                                     <  1:982962/150‑1 (MQ=255)
                                                       cTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGAc                                                                                                <  2:1305137/150‑1 (MQ=255)
                                                       cTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGAc                                                                                                <  2:362319/150‑1 (MQ=255)
                                                       cTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGAc                                                                                                <  1:1503519/150‑1 (MQ=255)
                                                       cTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGAc                                                                                                <  1:267055/150‑1 (MQ=255)
                                                        tGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACa                                                                                               <  1:3250957/150‑1 (MQ=255)
                                                        tGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACa                                                                                               >  2:3640103/1‑150 (MQ=255)
                                                          ggCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAAt                                                                                             <  1:4326326/150‑1 (MQ=255)
                                                           gCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATg                                                                                            <  2:3008883/150‑1 (MQ=255)
                                                                aCGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTc                                                                                       >  2:1765081/1‑150 (MQ=255)
                                                                  gCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGa                                                                                     <  1:399176/150‑1 (MQ=255)
                                                                  gCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGa                                                                                     >  1:854664/1‑150 (MQ=255)
                                                                    aTCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGAcc                                                                                   >  2:1948767/1‑150 (MQ=255)
                                                                    aTCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGAcc                                                                                   >  1:427872/1‑150 (MQ=255)
                                                                         cTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCAtata                                                                              <  1:218060/150‑1 (MQ=255)
                                                                          tGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAg                                                                             >  2:1832975/1‑150 (MQ=255)
                                                                            ggCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTc                                                                           >  1:3876843/1‑150 (MQ=255)
                                                                            ggCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTc                                                                           <  1:3610690/150‑1 (MQ=255)
                                                                             gCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCa                                                                          >  2:625718/1‑150 (MQ=255)
                                                                                acaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACg                                                                       <  2:3590892/150‑1 (MQ=255)
                                                                                acaaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACg                                                                       <  2:1322498/150‑1 (MQ=255)
                                                                                 caaATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGa                                                                      >  2:3993985/1‑150 (MQ=255)
                                                                                  aaaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaa                                                                     >  2:49540/1‑150 (MQ=255)
                                                                                  aaaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaa                                                                     >  1:1552473/1‑150 (MQ=255)
                                                                                  aaaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaa                                                                     >  2:2264575/1‑150 (MQ=255)
                                                                                   aaTTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGaaa                                                                    >  1:2317773/1‑150 (MQ=255)
                                                                                        cGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAAt                                                               <  2:2072594/150‑1 (MQ=255)
                                                                                          tGATCGTCATTTGCTTATTTTTAAGCATGGCGGCGACCTGTGTGTTACGCATCCCTTCCGCCATAATTTCAAACACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATcc                                                             <  1:883442/150‑1 (MQ=255)
                                                                                          tGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATcc                                                             <  2:1693124/150‑1 (MQ=255)
                                                                                          tGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATcc                                                             >  2:3435238/1‑150 (MQ=255)
                                                                                           gATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATccc                                                            >  1:3093486/1‑150 (MQ=255)
                                                                                           gATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATccc                                                            >  2:2532863/1‑150 (MQ=255)
                                                                                               gTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCa                                                        >  2:3423451/1‑150 (MQ=255)
                                                                                                tCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCaa                                                       >  1:2331872/1‑150 (MQ=255)
                                                                                                 cATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAAt                                                      <  1:1882237/150‑1 (MQ=255)
                                                                                                 cATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAAt                                                      >  2:280483/1‑150 (MQ=255)
                                                                                                 cATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAAt                                                      <  1:738085/150‑1 (MQ=255)
                                                                                                     tGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGt                                                  <  1:3816591/150‑1 (MQ=255)
                                                                                                     tGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGt                                                  >  2:168652/1‑150 (MQ=255)
                                                                                                         tATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTccc                                              >  1:1820895/1‑150 (MQ=255)
                                                                                                         tATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTccc                                              <  1:1952848/150‑1 (MQ=255)
                                                                                                         tATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTccc                                              <  1:4354464/150‑1 (MQ=255)
                                                                                                          aTTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCg                                             >  2:3067015/1‑150 (MQ=255)
                                                                                                          aTTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCg                                             >  2:893380/1‑150 (MQ=255)
                                                                                                              ttAAGCATGGCGGCGACCGGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGaaa                                         >  2:4385007/1‑150 (MQ=255)
                                                                                                               tAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCTCCATAATTTCAACCACCAGCTCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAAc                                        <  2:4260850/150‑1 (MQ=255)
                                                                                                               tAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAAc                                        >  2:1993012/1‑150 (MQ=255)
                                                                                                                 aGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACgg                                      >  2:799969/1‑150 (MQ=255)
                                                                                                                 aGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACgg                                      >  1:995622/1‑150 (MQ=255)
                                                                                                                 aGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACgg                                      >  2:4215053/1‑150 (MQ=255)
                                                                                                                 aGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACgg                                      >  2:4214928/1‑150 (MQ=255)
                                                                                                                      ggcggcGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAg                                 >  1:1689377/1‑150 (MQ=255)
                                                                                                                       gcggcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTTAATCGGTTCCCGGAAACGGGCCAgc                                <  2:3359944/150‑1 (MQ=255)
                                                                                                                        cggcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAgcg                               >  2:3925889/1‑150 (MQ=255)
                                                                                                                          gcgACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGcc                             <  2:484219/150‑1 (MQ=255)
                                                                                                                            gACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCct                           >  2:2142205/1‑150 (MQ=255)
                                                                                                                             aCCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCctc                          >  1:3189825/1‑150 (MQ=255)
                                                                                                                             aCCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCctc                          >  1:1082598/1‑150 (MQ=255)
                                                                                                                              ccTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCctct                         >  1:2824154/1‑150 (MQ=255)
                                                                                                                                     gTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTc                  <  2:4416769/150‑1 (MQ=255)
                                                                                                                                      ttACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTcc                 <  1:2634208/150‑1 (MQ=255)
                                                                                                                                          gCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTa             <  2:3861799/150‑1 (MQ=255)
                                                                                                                                           cATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTaa            <  1:1000315/150‑1 (MQ=255)
                                                                                                                                            aTCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAg           >  1:2386732/1‑150 (MQ=255)
                                                                                                                                              cccTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGAt         >  1:2739507/1‑150 (MQ=255)
                                                                                                                                              cccTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGAt         >  1:1861864/1‑150 (MQ=255)
                                                                                                                                                cTTCCGCAATAAGTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATaa       <  2:2663740/150‑1 (MQ=255)
                                                                                                                                                 ttCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAAt      >  1:3822563/1‑150 (MQ=255)
                                                                                                                                                 ttCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAAt      <  2:120106/150‑1 (MQ=255)
                                                                                                                                                 ttCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAAt      >  2:2643997/1‑150 (MQ=255)
                                                                                                                                                   ccGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTACGATAATCg    >  2:4506401/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGTAAACGTGAAGTCATTGCCGTGTTGATGTCTGGGCTTATCTCGCCTTTTTGCTGGGCATCACGCATCCGCTGGGCAACAAATTCCGTGATCGTCATATGCTTATTTTTAAGCATGGCGGCGACCTGTGGGTTACGCATCCCTTCCGCCATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCC  >  CP009273/1690832‑1691130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A