Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,825,763 C→A 91.0% A159A (GCG→GCT)  astC ← succinylornithine transaminase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,825,7630CA91.0% 659.4 / 57.8 233A159A (GCG→GCT) astCsuccinylornithine transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (16/5);  new base A (110/102);  total (126/107)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 239 total aligned reads displayed.

ACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCG  >  CP009273/1825616‑1825908
                                                                                                                                                   |                                                                                                                                                 
aCCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGgagc                                                                                                                                                 >  2:877759/1‑150 (MQ=255)
aCCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGgagc                                                                                                                                                 >  1:1996584/1‑150 (MQ=255)
    tGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCaaaa                                                                                                                                             >  2:620663/1‑150 (MQ=255)
    tGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCaaaa                                                                                                                                             >  2:2383206/1‑150 (MQ=255)
      tAAAAACGCGGTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATc                                                                                                                                           <  2:3994306/150‑1 (MQ=255)
       aaaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATcc                                                                                                                                          <  1:887277/150‑1 (MQ=255)
       aaaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATcc                                                                                                                                          <  1:2973768/150‑1 (MQ=255)
        aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCt                                                                                                                                         >  1:1517637/1‑150 (MQ=255)
        aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCt                                                                                                                                         >  1:1518453/1‑150 (MQ=255)
         aaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCtg                                                                                                                                        >  1:678109/1‑150 (MQ=255)
            cgcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGa                                                                                                                                     <  1:1582759/150‑1 (MQ=255)
            cgcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGa                                                                                                                                     <  1:1582789/150‑1 (MQ=255)
            cgcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGa                                                                                                                                     >  2:1321035/1‑150 (MQ=255)
            cgcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGa                                                                                                                                     <  2:4129290/150‑1 (MQ=255)
              cgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAAt                                                                                                                                   <  1:2648835/150‑1 (MQ=255)
                    tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctg                                                                                                                             >  2:2268755/1‑150 (MQ=255)
                                 acCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGc                                                                                                                >  1:2067698/1‑150 (MQ=255)
                                 acCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATAGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGc                                                                                                                >  1:4312563/1‑150 (MQ=255)
                                  ccGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCa                                                                                                               <  1:2547245/150‑1 (MQ=255)
                                  ccGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCa                                                                                                               <  2:3647344/150‑1 (MQ=255)
                                    gCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACt                                                                                                             >  1:11505/1‑150 (MQ=255)
                                      cTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGa                                                                                                           <  2:562924/150‑1 (MQ=255)
                                       ttCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGAc                                                                                                          <  2:3548430/150‑1 (MQ=255)
                                       ttCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGAc                                                                                                          <  2:1277346/150‑1 (MQ=255)
                                           cccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTa                                                                                                      >  1:1053305/1‑150 (MQ=255)
                                            ccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCGGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTaa                                                                                                     <  2:11725/150‑1 (MQ=255)
                                             cTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGGAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTaaa                                                                                                    <  1:4476098/150‑1 (MQ=255)
                                               ggATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACa                                                                                                  <  1:1810244/150‑1 (MQ=255)
                                                gATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAg                                                                                                 >  2:3349736/1‑150 (MQ=255)
                                                 aTGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGc                                                                                                <  2:2981730/150‑1 (MQ=255)
                                                     gTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGc                                                                                            <  2:2991204/150‑1 (MQ=255)
                                                        cGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                                                         >  2:1914238/1‑150 (MQ=255)
                                                        cGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                                                         >  2:3850626/1‑150 (MQ=255)
                                                        cGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                                                         >  1:3648308/1‑150 (MQ=255)
                                                         gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAcc                                                                                        >  1:238198/1‑150 (MQ=255)
                                                          aCAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCa                                                                                       <  2:3138020/150‑1 (MQ=255)
                                                           cAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCAt                                                                                      >  2:3333636/1‑150 (MQ=255)
                                                            aaTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATg                                                                                     <  2:2543956/150‑1 (MQ=255)
                                                            aaTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATg                                                                                     <  2:3980487/150‑1 (MQ=255)
                                                                aCCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTTTATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAc                                                                                 <  2:4326196/150‑1 (MQ=255)
                                                                aCCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAc                                                                                 <  1:4260147/150‑1 (MQ=255)
                                                                  cGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGc                                                                               >  2:1363895/1‑150 (MQ=255)
                                                                    cacaGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAt                                                                             >  2:3799867/1‑150 (MQ=255)
                                                                    cacaGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAt                                                                             <  2:4357305/150‑1 (MQ=255)
                                                                        ggTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttttt                                                                         <  2:1672131/150‑1 (MQ=255)
                                                                         gTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTg                                                                        >  1:3612790/1‑150 (MQ=255)
                                                                          tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                       >  1:245528/1‑150 (MQ=255)
                                                                          tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                       >  1:3356401/1‑150 (MQ=255)
                                                                          tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                       >  1:3356315/1‑150 (MQ=255)
                                                                                cgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCa                                                                 >  2:222320/1‑150 (MQ=255)
                                                                                 gtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAACCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAc                                                                >  2:1877893/1‑150 (MQ=255)
                                                                                    aaTCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAt                                                             >  2:2161344/1‑150 (MQ=255)
                                                                                      tCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGc                                                           <  2:3718253/150‑1 (MQ=255)
                                                                                        aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCg                                                         >  1:2705744/1‑150 (MQ=255)
                                                                                        aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCg                                                         >  1:2705759/1‑150 (MQ=255)
                                                                                        aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCg                                                         >  1:1216935/1‑150 (MQ=255)
                                                                                        aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCg                                                         >  1:2705816/1‑150 (MQ=255)
                                                                                            cgcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctct                                                     <  1:2167254/150‑1 (MQ=255)
                                                                                              cTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTa                                                   >  1:2132368/1‑150 (MQ=255)
                                                                                               tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                  >  2:225410/1‑150 (MQ=255)
                                                                                               tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTGAt                                                  >  2:3823373/1‑150 (MQ=255)
                                                                                                ggCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCCTGAAACGCATTTGTGAACGCCACGATGCCGCTCTTATg                                                 >  2:2412374/1‑150 (MQ=255)
                                                                                                ggCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATg                                                 <  1:2296821/150‑1 (MQ=255)
                                                                                                  cAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGc                                               >  2:2832191/1‑150 (MQ=255)
                                                                                                   agagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCt                                              <  2:197674/150‑1 (MQ=255)
                                                                                                    gagTTTATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATACTGTGACTAGGCTTGCTGCCCACCCTCACTGACAGTACACCTCGGGCGACCATGCACCGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTg                                             <  2:2877258/150‑1 (MQ=255)
                                                                                                    gagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTg                                             <  2:1518453/150‑1 (MQ=255)
                                                                                                    gagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTg                                             <  2:1517637/150‑1 (MQ=255)
                                                                                                    gagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTg                                             >  2:4092238/1‑150 (MQ=255)
                                                                                                    gagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTg                                             <  2:589509/150‑1 (MQ=255)
                                                                                                      gTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGcc                                           <  2:1088568/150‑1 (MQ=255)
                                                                                                      gTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGcc                                           <  1:2053869/150‑1 (MQ=255)
                                                                                                        tAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGt                                         >  2:1350826/1‑150 (MQ=255)
                                                                                                        tAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGt                                         >  1:3280358/1‑150 (MQ=255)
                                                                                                        tAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGt                                         >  1:3800150/1‑150 (MQ=255)
                                                                                                        tAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGt                                         >  1:3801615/1‑150 (MQ=255)
                                                                                                         aaTATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                        >  2:2044488/1‑150 (MQ=255)
                                                                                                         aaTATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                        >  2:3031609/1‑150 (MQ=255)
                                                                                                          atatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAg                                       >  2:1101256/1‑150 (MQ=255)
                                                                                                           tatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGc                                      >  1:4323276/1‑150 (MQ=255)
                                                                                                            atCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCg                                     <  2:3119833/150‑1 (MQ=255)
                                                                                                            atCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCg                                     >  1:2228432/1‑150 (MQ=255)
                                                                                                             tCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCgg                                    >  2:4201365/1‑150 (MQ=255)
                                                                                                             tCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCgg                                    >  1:3944113/1‑150 (MQ=255)
                                                                                                               gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTc                                  <  1:859360/150‑1 (MQ=255)
                                                                                                                       cagcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGa                          >  2:2156532/1‑150 (MQ=255)
                                                                                                                        agcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGaa                         >  1:107665/1‑150 (MQ=255)
                                                                                                                           aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAttt                      <  2:77454/150‑1 (MQ=255)
                                                                                                                             gACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAc                    <  2:3650386/150‑1 (MQ=255)
                                                                                                                             gACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAc                    <  2:1247452/150‑1 (MQ=255)
                                                                                                                              aCGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcg                   <  2:1236223/150‑1 (MQ=255)
                                                                                                                               cGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcgc                  <  2:119464/150‑1 (MQ=255)
                                                                                                                                 aaTATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACgcgc                >  1:1969747/1‑150 (MQ=255)
                                                                                                                                     tCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGt            <  2:3259667/150‑1 (MQ=255)
                                                                                                                                       cgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGttt          <  1:4170129/150‑1 (MQ=255)
                                                                                                                                        gccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGtttt         <  2:3874756/150‑1 (MQ=255)
                                                                                                                                         ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTa        >  2:2328616/1‑150 (MQ=255)
                                                                                                                                         ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTa        <  1:293197/150‑1 (MQ=255)
                                                                                                                                         ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTa        >  2:2327313/1‑150 (MQ=255)
                                                                                                                                             cAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAgcgc    >  2:1751027/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCG  >  CP009273/1825616‑1825908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTC  >  CP009273/1825615‑1825912
                                                                                                                                                    |                                                                                                                                                     
GACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAG                                                                                                                                                      >  SRR19973076.1090189/1‑150 (MQ=60)
GACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAG                                                                                                                                                      >  SRR19973076.1090202/1‑150 (MQ=60)
 ACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGC                                                                                                                                                     >  SRR19973076.1999487/1‑150 (MQ=60)
 ACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGC                                                                                                                                                     <  SRR19973076.951270/150‑1 (MQ=60)
  CCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCA                                                                                                                                                    <  SRR19973076.1075581/150‑1 (MQ=60)
  CCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCA                                                                                                                                                    >  SRR19973076.2044313/1‑150 (MQ=60)
  CCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCA                                                                                                                                                    >  SRR19973076.2044275/1‑150 (MQ=60)
      GTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAT                                                                                                                                                <  SRR19973076.791926/150‑1 (MQ=60)
        AAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCC                                                                                                                                              <  SRR19973076.2978094/150‑1 (MQ=60)
        AAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCC                                                                                                                                              <  SRR19973076.888591/150‑1 (MQ=60)
         AAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCT                                                                                                                                             >  SRR19973076.1519869/1‑150 (MQ=60)
         AAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCT                                                                                                                                             >  SRR19973076.1519908/1‑150 (MQ=60)
         AAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCT                                                                                                                                             >  SRR19973076.1520686/1‑150 (MQ=60)
         AAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCT                                                                                                                                             >  SRR19973076.1520689/1‑150 (MQ=60)
          AAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTG                                                                                                                                            >  SRR19973076.679124/1‑150 (MQ=60)
          AAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTG                                                                                                                                            >  SRR19973076.2271519/1‑150 (MQ=60)
             CGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGA                                                                                                                                         <  SRR19973076.2850002/150‑1 (MQ=60)
             CGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGA                                                                                                                                         <  SRR19973076.1585113/150‑1 (MQ=60)
             CGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGA                                                                                                                                         <  SRR19973076.1585085/150‑1 (MQ=60)
             CGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGA                                                                                                                                         <  SRR19973076.1585083/150‑1 (MQ=60)
               CGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAAT                                                                                                                                       <  SRR19973076.2652688/150‑1 (MQ=60)
                GTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATA                                                                                                                                      <  SRR19973076.1632286/150‑1 (MQ=60)
                GTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATA                                                                                                                                      <  SRR19973076.1632467/150‑1 (MQ=60)
                  TGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGG                                                                                                                                    <  SRR19973076.3801770/150‑1 (MQ=60)
                     TGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTG                                                                                                                                 <  SRR19973076.2133754/150‑1 (MQ=60)
                     TGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTG                                                                                                                                 <  SRR19973076.2133769/150‑1 (MQ=60)
                                  ACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGC                                                                                                                    >  SRR19973076.2070707/1‑150 (MQ=60)
                                  ACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATAGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGC                                                                                                                    >  SRR19973076.4318929/1‑150 (MQ=60)
                                   CCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCTGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCA                                                                                                                   <  SRR19973076.251683/150‑1 (MQ=60)
                                   CCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCA                                                                                                                   <  SRR19973076.2550950/150‑1 (MQ=60)
                                     GCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACT                                                                                                                 >  SRR19973076.11528/1‑150 (MQ=60)
                                           CCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGT                                                                                                           >  SRR19973076.4007192/1‑150 (MQ=60)
                                            CCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTA                                                                                                          >  SRR19973076.1054867/1‑150 (MQ=60)
                                             CCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAA                                                                                                         <  SRR19973076.1403355/150‑1 (MQ=60)
                                              CTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGGAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAA                                                                                                        <  SRR19973076.4482761/150‑1 (MQ=60)
                                               TGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAC                                                                                                       <  SRR19973076.1136062/150‑1 (MQ=60)
                                                GGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACA                                                                                                      <  SRR19973076.1812851/150‑1 (MQ=60)
                                                         CGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAC                                                                                             >  SRR19973076.3654790/1‑150 (MQ=60)
                                                         CGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAC                                                                                             >  SRR19973076.3653613/1‑150 (MQ=60)
                                                         CGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAC                                                                                             >  SRR19973076.3653611/1‑150 (MQ=60)
                                                         CGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAC                                                                                             >  SRR19973076.2635384/1‑150 (MQ=60)
                                                          GACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACC                                                                                            >  SRR19973076.119681/1‑150 (MQ=60)
                                                          GACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACC                                                                                            >  SRR19973076.241162/1‑150 (MQ=60)
                                                          GACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACC                                                                                            >  SRR19973076.238603/1‑150 (MQ=60)
                                                           ACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCA                                                                                           <  SRR19973076.3396612/150‑1 (MQ=60)
                                                                CACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCACCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAA                                                                                      >  SRR19973076.985032/1‑150 (MQ=60)
                                                                 ACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAC                                                                                     <  SRR19973076.2912672/150‑1 (MQ=60)
                                                                 ACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAC                                                                                     <  SRR19973076.582878/150‑1 (MQ=60)
                                                                 ACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAC                                                                                     <  SRR19973076.4266441/150‑1 (MQ=60)
                                                                  CCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACG                                                                                    <  SRR19973076.1543430/150‑1 (MQ=60)
                                                                      ACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATT                                                                                >  SRR19973076.223065/1‑150 (MQ=60)
                                                                      ACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATT                                                                                >  SRR19973076.223311/1‑150 (MQ=60)
                                                                      ACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATT                                                                                >  SRR19973076.3077332/1‑150 (MQ=60)
                                                                      ACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGGAATCAGCGTGCGACCATGAAACGCATT                                                                                >  SRR19973076.3076447/1‑150 (MQ=60)
                                                                          GTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTG                                                                            >  SRR19973076.3618040/1‑150 (MQ=60)
                                                                           TAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGA                                                                           >  SRR19973076.3361187/1‑150 (MQ=60)
                                                                           TAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGA                                                                           >  SRR19973076.245944/1‑150 (MQ=60)
                                                                           TAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGA                                                                           >  SRR19973076.3361132/1‑150 (MQ=60)
                                                                           TAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGA                                                                           >  SRR19973076.3361227/1‑150 (MQ=60)
                                                                           TAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGA                                                                           >  SRR19973076.3361273/1‑150 (MQ=60)
                                                                                     AATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAT                                                                 >  SRR19973076.3897285/1‑150 (MQ=60)
                                                                                     AATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAT                                                                 >  SRR19973076.2448273/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.2709751/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.2709699/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.2709694/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.2709687/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.2709679/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.2709505/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.1218741/1‑150 (MQ=60)
                                                                                         AGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCG                                                             >  SRR19973076.1217393/1‑150 (MQ=60)
                                                                                             CGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCT                                                         <  SRR19973076.2170384/150‑1 (MQ=60)
                                                                                               CTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTA                                                       >  SRR19973076.2135630/1‑150 (MQ=60)
                                                                                               CTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTA                                                       >  SRR19973076.2135460/1‑150 (MQ=60)
                                                                                                 GGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATG                                                     <  SRR19973076.2300137/150‑1 (MQ=60)
                                                                                                 GGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATG                                                     <  SRR19973076.2300111/150‑1 (MQ=60)
                                                                                                       GTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCC                                               <  SRR19973076.2056884/150‑1 (MQ=60)
                                                                                                       GTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCC                                               <  SRR19973076.4305491/150‑1 (MQ=60)
                                                                                                       GTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCC                                               <  SRR19973076.2056865/150‑1 (MQ=60)
                                                                                                       GTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCC                                               <  SRR19973076.4305341/150‑1 (MQ=60)
                                                                                                         TAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGT                                             >  SRR19973076.3285118/1‑150 (MQ=60)
                                                                                                         TAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGT                                             >  SRR19973076.3805727/1‑150 (MQ=60)
                                                                                                         TAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGT                                             >  SRR19973076.3807185/1‑150 (MQ=60)
                                                                                                         TAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGT                                             >  SRR19973076.3807192/1‑150 (MQ=60)
                                                                                                           ATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAG                                           >  SRR19973076.540066/1‑150 (MQ=60)
                                                                                                            TATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGC                                          >  SRR19973076.4329564/1‑150 (MQ=60)
                                                                                                            TATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGC                                          >  SRR19973076.4329664/1‑150 (MQ=60)
                                                                                                             ATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCG                                         >  SRR19973076.2231637/1‑150 (MQ=60)
                                                                                                              TCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGG                                        >  SRR19973076.4035828/1‑150 (MQ=60)
                                                                                                              TCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGG                                        >  SRR19973076.3949931/1‑150 (MQ=60)
                                                                                                               CGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGT                                       >  SRR19973076.4386455/1‑150 (MQ=60)
                                                                                                                GTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTC                                      <  SRR19973076.860625/150‑1 (MQ=60)
                                                                                                                GTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTC                                      <  SRR19973076.860629/150‑1 (MQ=60)
                                                                                                                         AGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAA                             >  SRR19973076.107869/1‑150 (MQ=60)
                                                                                                                          GCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAT                            <  SRR19973076.3533508/150‑1 (MQ=60)
                                                                                                                           CATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATT                           >  SRR19973076.1931991/1‑150 (MQ=60)
                                                                                                                           CATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATT                           <  SRR19973076.1985097/150‑1 (MQ=60)
                                                                                                                              GACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAC                        <  SRR19973076.4242949/150‑1 (MQ=60)
                                                                                                                                  AATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGC                    >  SRR19973076.1972614/1‑150 (MQ=60)
                                                                                                                                        CGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTT              <  SRR19973076.4176289/150‑1 (MQ=60)
                                                                                                                                          CCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTA            <  SRR19973076.293670/150‑1 (MQ=60)
                                                                                                                                          CCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTA            <  SRR19973076.293810/150‑1 (MQ=60)
                                                                                                                                               AGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCC       <  SRR19973076.2678355/150‑1 (MQ=60)
                                                                                                                                                  GGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCT    <  SRR19973076.1424884/150‑1 (MQ=60)
                                                                                                                                                  GGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAAGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCT    <  SRR19973076.3697915/150‑1 (MQ=60)
                                                                                                                                                    AGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTC  <  SRR19973076.2677148/150‑1 (MQ=60)
                                                                                                                                                    AGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTC  <  SRR19973076.2677132/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GACCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCTTC  >  CP009273/1825615‑1825912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑