Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,865,422 C→A 88.4% R261R (CGA→AGA)  yeaI → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,865,4220CA88.4% 664.8 / 80.4 241R261R (CGA→AGA) yeaIputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (10/18);  new base A (115/98);  total (125/116)
Fisher's exact test for biased strand distribution p-value = 7.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 241 total aligned reads displayed.

CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGT  >  CP009273/1865275‑1865567
                                                                                                                                                    |                                                                                                                                                 
  caATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGAtt                                                                                                                                               <  1:3156641/150‑1 (MQ=255)
   aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTTCTGTTGTTTATTATAATGCGCACAAGATTa                                                                                                                                              >  1:3075186/1‑150 (MQ=255)
   aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                              <  1:4246401/150‑1 (MQ=255)
   aaTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTa                                                                                                                                              >  2:3515687/1‑150 (MQ=255)
    aTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAg                                                                                                                                             <  1:2739293/150‑1 (MQ=255)
    aTCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAg                                                                                                                                             >  2:1646470/1‑150 (MQ=255)
       tGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCa                                                                                                                                          >  2:1854160/1‑150 (MQ=255)
            aGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGaa                                                                                                                                     >  1:3128434/1‑150 (MQ=255)
               tataACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTa                                                                                                                                  <  2:1921501/150‑1 (MQ=255)
               tataACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTa                                                                                                                                  <  2:2165350/150‑1 (MQ=255)
                ataACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTAt                                                                                                                                 >  1:4166273/1‑150 (MQ=255)
                 taACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATg                                                                                                                                <  2:4466530/150‑1 (MQ=255)
                 taACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATg                                                                                                                                <  2:4466506/150‑1 (MQ=255)
                  aaCGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATgg                                                                                                                               <  1:302212/150‑1 (MQ=255)
                    cGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGcc                                                                                                                             >  2:3763354/1‑150 (MQ=255)
                        gACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTa                                                                                                                         >  2:1652965/1‑150 (MQ=255)
                        gACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTa                                                                                                                         >  1:2801377/1‑150 (MQ=255)
                          cTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCCGCGAACTATGGCCGTTAAt                                                                                                                       >  1:2028739/1‑150 (MQ=255)
                           tATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATa                                                                                                                      <  2:4447469/150‑1 (MQ=255)
                              tCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCa                                                                                                                   >  1:352502/1‑150 (MQ=255)
                               cTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATATCat                                                                                                                  >  2:3152309/1‑150 (MQ=255)
                                tttGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCatt                                                                                                                 <  1:1832120/150‑1 (MQ=255)
                                  tGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTCTGGCCGTTAATAGCattat                                                                                                               >  2:2609261/1‑150 (MQ=255)
                                  tGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCattat                                                                                                               <  1:1658659/150‑1 (MQ=255)
                                   gTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCattatt                                                                                                              <  2:1043135/150‑1 (MQ=255)
                                     aTGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATtattat                                                                                                            <  2:3904732/150‑1 (MQ=255)
                                       gtcgtcGATTACTGTCCGGACAACCATACTGCGACTTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGt                                                                                                          <  1:3999561/150‑1 (MQ=255)
                                         cgtcgATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCt                                                                                                        >  2:3924921/1‑150 (MQ=255)
                                                tACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCg                                                                                                 >  1:3264119/1‑150 (MQ=255)
                                                tACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCg                                                                                                 <  2:1897900/150‑1 (MQ=255)
                                                tACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCg                                                                                                 >  2:1184433/1‑150 (MQ=255)
                                                  cTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCt                                                                                               <  1:1025500/150‑1 (MQ=255)
                                                   tGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTa                                                                                              >  2:2846099/1‑150 (MQ=255)
                                                   tGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTa                                                                                              >  1:2774071/1‑150 (MQ=255)
                                                   tGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTa                                                                                              >  1:3419769/1‑150 (MQ=255)
                                                   tGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTa                                                                                              >  1:2391644/1‑150 (MQ=255)
                                                    gTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAt                                                                                             <  1:485379/150‑1 (MQ=255)
                                                     ttcGGTCAAACCTACTGCGAACTGGGGTATCAAATATACAAAAATTTTGGTTTGTATGTGGGCATTTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAt                                                                                             <  1:376175/148‑1 (MQ=255)
                                                             aCCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAact                                                                                    >  2:3310577/1‑150 (MQ=255)
                                                               cATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAactta                                                                                  <  2:363905/150‑1 (MQ=255)
                                                                aTACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAacttac                                                                                 >  2:1317002/1‑150 (MQ=255)
                                                                 tACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAacttact                                                                                <  1:2277302/150‑1 (MQ=255)
                                                                  aCTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAacttactt                                                                               >  2:251898/1‑150 (MQ=255)
                                                                    tGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCt                                                                             <  2:91204/150‑1 (MQ=255)
                                                                    tGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATAGCTATGCTGCAACTTACTTCt                                                                             >  1:2694952/1‑150 (MQ=255)
                                                                      cGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                           >  2:1562808/1‑150 (MQ=255)
                                                                      cGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTAc                                                                           >  2:3450887/1‑150 (MQ=255)
                                                                       gACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACt                                                                          <  1:3797396/150‑1 (MQ=255)
                                                                             gggAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGActct                                                                    >  1:1032066/1‑150 (MQ=255)
                                                                              ggAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTg                                                                   >  2:1348892/1‑150 (MQ=255)
                                                                               gAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTgg                                                                  <  2:2676206/150‑1 (MQ=255)
                                                                                 aTCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAt                                                                >  1:3942293/1‑150 (MQ=255)
                                                                                  tCAACTATACAAAAATATTGGTTTGTCTGTGGTCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATg                                                               <  1:1563329/150‑1 (MQ=255)
                                                                                     aCTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTCATAGCATTATTATGTCTGGCATCGCGATGCTGCAACTTACTTCTACTGACTCGGGAGGAGt                                                            >  2:4194112/1‑150 (MQ=255)
                                                                                     aCTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATTCTGCAACTTACTTCTACTGACTCTGGATTAGt                                                            >  2:2078076/1‑150 (MQ=255)
                                                                                        ataCAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGtata                                                         <  1:2652592/150‑1 (MQ=255)
                                                                                        ataCAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGtata                                                         >  1:145394/1‑150 (MQ=255)
                                                                                            aaaaaTATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATta                                                     >  1:718935/1‑150 (MQ=255)
                                                                                            aaaaaTATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATta                                                     >  1:2983201/1‑150 (MQ=255)
                                                                                            aaaaaTATTGGTTTGTCTGTGGGCA‑TTTCTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATta                                                     <  1:1149932/150‑1 (MQ=255)
                                                                                                   ttGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATc                                              <  1:1591618/150‑1 (MQ=255)
                                                                                                    tGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATATCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCt                                             >  1:3457628/1‑150 (MQ=255)
                                                                                                        ttGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                         >  1:4509507/1‑150 (MQ=255)
                                                                                                        ttGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta                                         >  1:4509275/1‑150 (MQ=255)
                                                                                                         tGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtat                                        >  1:2533904/1‑150 (MQ=255)
                                                                                                             tgtgGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCa                                    <  1:3914894/150‑1 (MQ=255)
                                                                                                              gtgGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAg                                   >  2:3298643/1‑150 (MQ=255)
                                                                                                                gggCC‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTc                                 >  1:4501240/1‑150 (MQ=255)
                                                                                                                gggCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATATGTTATATCAGTc                                 >  2:3226255/1‑150 (MQ=255)
                                                                                                                 ggCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcg                                <  1:1434031/150‑1 (MQ=255)
                                                                                                                   cA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcgcg                              >  2:2387281/1‑150 (MQ=255)
                                                                                                                   cA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcgcg                              <  2:405457/150‑1 (MQ=255)
                                                                                                                   cA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcgcg                              <  1:1331132/150‑1 (MQ=255)
                                                                                                                   cA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcgag                              >  2:2663074/1‑148 (MQ=255)
                                                                                                                    a‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGGATAATTATAATATCTGGTATATCAGTCTCgg                             >  2:3295148/1‑150 (MQ=255)
                                                                                                                        tttACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAt                          <  2:2810168/150‑1 (MQ=255)
                                                                                                                        tttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAt                          >  2:1560685/1‑150 (MQ=255)
                                                                                                                        tttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAt                          >  2:926887/1‑150 (MQ=255)
                                                                                                                         ttACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAtt                         <  1:873547/150‑1 (MQ=255)
                                                                                                                         ttACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAtt                         <  1:3565283/150‑1 (MQ=255)
                                                                                                                          tccTGTTCTTTAGTAGCCGGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCGGGCATCGCGATGCTGCAACTTACTGCTACTGACTCTGGATGAGTATAATTATACTATCTGGTAGATCAGTCGCGGGATTg                        <  2:548392/148‑1 (MQ=255)
                                                                                                                            cTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGaa                      >  2:739809/1‑150 (MQ=255)
                                                                                                                            cTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGaa                      <  2:5069/150‑1 (MQ=255)
                                                                                                                             tGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAg                     <  1:4050296/150‑1 (MQ=255)
                                                                                                                                 cTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTc                 >  1:1100671/1‑150 (MQ=255)
                                                                                                                                 cTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTc                 >  1:350108/1‑150 (MQ=255)
                                                                                                                                  tttATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTcc                <  1:3779860/150‑1 (MQ=255)
                                                                                                                                     attatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGt             >  1:3886666/1‑150 (MQ=255)
                                                                                                                                     attatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGt             >  1:1222829/1‑150 (MQ=255)
                                                                                                                                      ttatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTa            >  1:708490/1‑150 (MQ=255)
                                                                                                                                      ttatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTa            <  2:301682/150‑1 (MQ=255)
                                                                                                                                       tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa           >  1:1298409/1‑150 (MQ=255)
                                                                                                                                       tatCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa           <  2:4113124/150‑1 (MQ=255)
                                                                                                                                         tCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAAc         <  1:700451/150‑1 (MQ=255)
                                                                                                                                          cATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACt        <  2:757368/150‑1 (MQ=255)
                                                                                                                                          cATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACt        <  2:4173158/150‑1 (MQ=255)
                                                                                                                                              cgcACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgttt    <  2:65223/150‑1 (MQ=255)
                                                                                                                                              cgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgttt    <  1:3417110/150‑1 (MQ=255)
                                                                                                                                              cgcACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgttt    <  2:1384647/150‑1 (MQ=255)
                                                                                                                                                cacaAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttgt  <  2:3790773/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGT  >  CP009273/1865275‑1865567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGT  >  CP009273/1865273‑1865570
                                                                                                                                                      |                                                                                                                                                    
CACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAA                                                                                                                                                      >  SRR19973080.530763/1‑150 (MQ=60)
 ACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAG                                                                                                                                                     <  SRR19973080.2570234/150‑1 (MQ=60)
 ACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAG                                                                                                                                                     <  SRR19973080.1206276/150‑1 (MQ=60)
    CAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATT                                                                                                                                                  <  SRR19973080.3174029/150‑1 (MQ=60)
     AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTTCTGTTGTTTATTATAATGCGCACAAGATTA                                                                                                                                                 >  SRR19973080.3091948/1‑150 (MQ=60)
     AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTA                                                                                                                                                 <  SRR19973080.1127626/150‑1 (MQ=60)
     AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTA                                                                                                                                                 <  SRR19973080.4269570/150‑1 (MQ=60)
     AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTA                                                                                                                                                 <  SRR19973080.2687786/150‑1 (MQ=60)
     AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTA                                                                                                                                                 <  SRR19973080.2687755/150‑1 (MQ=60)
     AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTA                                                                                                                                                 <  SRR19973080.469790/150‑1 (MQ=60)
     AATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCAAACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTAATGTTATTTATTATCATTCGCACAATATTA                                                                                                                                                 >  SRR19973080.3091355/1‑150 (MQ=60)
      ATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAG                                                                                                                                                <  SRR19973080.2754366/150‑1 (MQ=60)
      ATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAG                                                                                                                                                <  SRR19973080.3523726/150‑1 (MQ=60)
      ATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAG                                                                                                                                                <  SRR19973080.4086527/150‑1 (MQ=60)
      ATCTGAGCAGTTATAACGCTGACTAGTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAG                                                                                                                                                <  SRR19973080.2754231/150‑1 (MQ=60)
           AGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGC                                                                                                                                           >  SRR19973080.1266123/1‑150 (MQ=60)
            GCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCG                                                                                                                                          <  SRR19973080.3862628/150‑1 (MQ=60)
             CAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGA                                                                                                                                         >  SRR19973080.761631/1‑150 (MQ=60)
              AGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAA                                                                                                                                        >  SRR19973080.3145655/1‑150 (MQ=60)
                  ATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTAT                                                                                                                                    >  SRR19973080.4188778/1‑150 (MQ=60)
                    AACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGG                                                                                                                                  <  SRR19973080.304136/150‑1 (MQ=60)
                    AACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTGTATTATCATGCGCACACGATTAGCCAGCGAACTATGG                                                                                                                                  >  SRR19973080.1875720/1‑150 (MQ=60)
                       GCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCG                                                                                                                               >  SRR19973080.2999696/1‑150 (MQ=60)
                          GACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTA                                                                                                                            <  SRR19973080.4112945/150‑1 (MQ=60)
                          GACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTA                                                                                                                            >  SRR19973080.2816819/1‑150 (MQ=60)
                            CTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCCGCGAACTATGGCCGTTAAT                                                                                                                          >  SRR19973080.2040264/1‑150 (MQ=60)
                                TCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCA                                                                                                                      >  SRR19973080.354148/1‑150 (MQ=60)
                                TCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCA                                                                                                                      >  SRR19973080.354561/1‑150 (MQ=60)
                                  TTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATT                                                                                                                    <  SRR19973080.1842454/150‑1 (MQ=60)
                                  TTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATT                                                                                                                    <  SRR19973080.1842426/150‑1 (MQ=60)
                                  TTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATT                                                                                                                    <  SRR19973080.1311420/150‑1 (MQ=60)
                                   TTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTA                                                                                                                   <  SRR19973080.3926821/150‑1 (MQ=60)
                                    TGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTAT                                                                                                                  <  SRR19973080.1668353/150‑1 (MQ=60)
                                     GTATGTCGTCGATTACTGTCCGGACAACCATAATGCGACCTGGGGAATAAAATATACAAAAATATTTGTTTGTCTGTTGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTATCCAGCGAAATATTGCCGTTAATAGCATTATT                                                                                                                 >  SRR19973080.3462249/1‑150 (MQ=60)
                                         GTCGTCGATTACTGTCCGGACAACCATACTGCGACTTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGT                                                                                                             <  SRR19973080.4021530/150‑1 (MQ=60)
                                            GTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTG                                                                                                          >  SRR19973080.539178/1‑150 (MQ=60)
                                            GTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTG                                                                                                          >  SRR19973080.2419877/1‑150 (MQ=60)
                                                  TACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCG                                                                                                    <  SRR19973080.157479/150‑1 (MQ=60)
                                                  TACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCG                                                                                                    >  SRR19973080.3282060/1‑150 (MQ=60)
                                                  TACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCG                                                                                                    <  SRR19973080.158589/150‑1 (MQ=60)
                                                    CTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCT                                                                                                  <  SRR19973080.1031596/150‑1 (MQ=60)
                                                     TGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTA                                                                                                 >  SRR19973080.2404930/1‑150 (MQ=60)
                                                     TGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTA                                                                                                 >  SRR19973080.2789374/1‑150 (MQ=60)
                                                     TGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTA                                                                                                 >  SRR19973080.3438346/1‑150 (MQ=60)
                                                      GTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAT                                                                                                <  SRR19973080.2258843/150‑1 (MQ=60)
                                                      GTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAT                                                                                                <  SRR19973080.488469/150‑1 (MQ=60)
                                                       ttcggtcaaaccTACTGCGAACTGGGGTATCAAATATACAAAAATTTTGGTTTGTATGTGGGCATTTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTAT                                                                                                <  SRR19973080.378304/138‑1 (MQ=60)
                                                        CCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGatcagtgtact                                                                                              >  SRR19973080.4385635/1‑139 (MQ=60)
                                                        CCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGatcagtgtact                                                                                              >  SRR19973080.4385638/1‑139 (MQ=60)
                                                            ACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCA                                                                                          <  SRR19973080.3470751/150‑1 (MQ=60)
                                                              AACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAAC                                                                                        <  SRR19973080.3305413/150‑1 (MQ=60)
                                                                   TACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACT                                                                                   <  SRR19973080.2289777/150‑1 (MQ=60)
                                                                      TGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATAGCTATGCTGCAACTTACTTCT                                                                                >  SRR19973080.2709691/1‑150 (MQ=60)
                                                                       GCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTA                                                                               <  SRR19973080.232616/150‑1 (MQ=60)
                                                                         GACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACT                                                                             <  SRR19973080.3817498/150‑1 (MQ=60)
                                                                         GACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACT                                                                             <  SRR19973080.3818109/150‑1 (MQ=60)
                                                                         GACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACT                                                                             <  SRR19973080.3756606/150‑1 (MQ=60)
                                                                           CCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGA                                                                           >  SRR19973080.3554886/1‑150 (MQ=60)
                                                                               GGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCT                                                                       >  SRR19973080.1037588/1‑150 (MQ=60)
                                                                               GGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCT                                                                       >  SRR19973080.1038184/1‑150 (MQ=60)
                                                                                 GAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGG                                                                     <  SRR19973080.1243321/150‑1 (MQ=60)
                                                                                  AATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGA                                                                    <  SRR19973080.2893165/150‑1 (MQ=60)
                                                                                  AATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGA                                                                    >  SRR19973080.3166811/1‑150 (MQ=60)
                                                                                   ATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGAT                                                                   >  SRR19973080.3964025/1‑150 (MQ=60)
                                                                                    TCAACTATACAAAAATATTGGTTTGTCTGTGGTCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATG                                                                  <  SRR19973080.1572423/150‑1 (MQ=60)
                                                                                    TCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATG                                                                  <  SRR19973080.3864212/150‑1 (MQ=60)
                                                                                       ACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGT                                                               >  SRR19973080.3789932/1‑150 (MQ=60)
                                                                                         TATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTAT                                                             <  SRR19973080.327563/150‑1 (MQ=60)
                                                                                          ATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATA                                                            <  SRR19973080.3103009/150‑1 (MQ=60)
                                                                                          ATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATA                                                            >  SRR19973080.146581/1‑150 (MQ=60)
                                                                                          ATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATA                                                            <  SRR19973080.2666928/150‑1 (MQ=60)
                                                                                            ACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAAT                                                          >  SRR19973080.3136027/1‑150 (MQ=60)
                                                                                              AAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTA                                                        <  SRR19973080.3937060/150‑1 (MQ=60)
                                                                                              AAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTA                                                        >  SRR19973080.722992/1‑150 (MQ=60)
                                                                                              AAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTA                                                        >  SRR19973080.723077/1‑150 (MQ=60)
                                                                                              AAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTA                                                        >  SRR19973080.2999298/1‑150 (MQ=60)
                                                                                              AAAAATATTGGTTTGTCTGTGGGCA‑TTTCTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTA                                                        <  SRR19973080.1156708/150‑1 (MQ=60)
                                                                                                     TTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATC                                                 <  SRR19973080.1601396/150‑1 (MQ=60)
                                                                                                     TTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATC                                                 <  SRR19973080.1600924/150‑1 (MQ=60)
                                                                                                     TTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATC                                                 <  SRR19973080.1600916/150‑1 (MQ=60)
                                                                                                      TGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATATCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCT                                                >  SRR19973080.3476568/1‑150 (MQ=60)
                                                                                                          TTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTA                                            >  SRR19973080.4533614/1‑150 (MQ=60)
                                                                                                          TTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTA                                            >  SRR19973080.4533702/1‑150 (MQ=60)
                                                                                                          TTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTA                                            >  SRR19973080.4533846/1‑150 (MQ=60)
                                                                                                          TTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTA                                            >  SRR19973080.4534084/1‑150 (MQ=60)
                                                                                                           TGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTAT                                           >  SRR19973080.2547818/1‑150 (MQ=60)
                                                                                                            GTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATA                                          <  SRR19973080.978292/150‑1 (MQ=60)
                                                                                                              CTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATC                                        >  SRR19973080.1230002/1‑150 (MQ=60)
                                                                                                               TGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCA                                       <  SRR19973080.3936504/150‑1 (MQ=60)
                                                                                                                GTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAG                                      >  SRR19973080.3993460/1‑150 (MQ=60)
                                                                                                                  GGGCC‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTC                                    >  SRR19973080.4525553/1‑150 (MQ=60)
                                                                                                                  GGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATAATATCTGTTATATAAGTC                                    >  SRR19973080.4299822/1‑150 (MQ=60)
                                                                                                                  GGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTC                                    >  SRR19973080.4525529/1‑150 (MQ=60)
                                                                                                                  GGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTC                                    >  SRR19973080.4525576/1‑150 (MQ=60)
                                                                                                                   GGCA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCG                                   <  SRR19973080.1442414/150‑1 (MQ=60)
                                                                                                                     CA‑TTTTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCG                                 <  SRR19973080.1339158/150‑1 (MQ=60)
                                                                                                                          TTTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAT                             <  SRR19973080.3783856/150‑1 (MQ=60)
                                                                                                                           TTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATT                            <  SRR19973080.878878/150‑1 (MQ=60)
                                                                                                                           TTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATT                            <  SRR19973080.1113227/150‑1 (MQ=60)
                                                                                                                           TTACTGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATT                            <  SRR19973080.3585011/150‑1 (MQ=60)
                                                                                                                               TGTTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAG                        <  SRR19973080.4072449/150‑1 (MQ=60)
                                                                                                                                 TTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTT                      >  SRR19973080.2777445/1‑150 (MQ=60)
                                                                                                                                 TTCTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTT                      >  SRR19973080.4198267/1‑150 (MQ=60)
                                                                                                                                   CTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTC                    >  SRR19973080.352161/1‑150 (MQ=60)
                                                                                                                                   CTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTC                    >  SRR19973080.351382/1‑150 (MQ=60)
                                                                                                                                   CTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTC                    >  SRR19973080.1107001/1‑150 (MQ=60)
                                                                                                                                   CTTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTC                    >  SRR19973080.1106427/1‑150 (MQ=60)
                                                                                                                                    TTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCC                   <  SRR19973080.4010802/150‑1 (MQ=60)
                                                                                                                                    TTTATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCC                   <  SRR19973080.3800397/150‑1 (MQ=60)
                                                                                                                                      TATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAG                 <  SRR19973080.1452029/150‑1 (MQ=60)
                                                                                                                                       ATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGT                <  SRR19973080.2569077/150‑1 (MQ=60)
                                                                                                                                       ATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGT                >  SRR19973080.3908090/1‑150 (MQ=60)
                                                                                                                                       ATTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATAATATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGT                >  SRR19973080.1230225/1‑150 (MQ=60)
                                                                                                                                        TTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTA               >  SRR19973080.712609/1‑150 (MQ=60)
                                                                                                                                        TTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTA               >  SRR19973080.711561/1‑150 (MQ=60)
                                                                                                                                        TTATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTA               >  SRR19973080.711476/1‑150 (MQ=60)
                                                                                                                                         TATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAA              >  SRR19973080.1306263/1‑150 (MQ=60)
                                                                                                                                          ATCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAA             >  SRR19973080.1888409/1‑150 (MQ=60)
                                                                                                                                           TCATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAAC            <  SRR19973080.704548/150‑1 (MQ=60)
                                                                                                                                            CATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACT           <  SRR19973080.2857873/150‑1 (MQ=60)
                                                                                                                                            CATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACT           <  SRR19973080.1451969/150‑1 (MQ=60)
                                                                                                                                            CATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACT           >  SRR19973080.2804258/1‑150 (MQ=60)
                                                                                                                                             ATGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTG          >  SRR19973080.4057785/1‑150 (MQ=60)
                                                                                                                                              TGCGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGT         <  SRR19973080.1397522/150‑1 (MQ=60)
                                                                                                                                                CGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTT       <  SRR19973080.3435677/150‑1 (MQ=60)
                                                                                                                                                CGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTT       <  SRR19973080.3435659/150‑1 (MQ=60)
                                                                                                                                                CGCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTAATATGTATTGCCACGCTATTCTGCAACTTAAATATACATACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATAGAAGTTAACAGTAAACTGTTT       >  SRR19973080.2396609/1‑150 (MQ=60)
                                                                                                                                                 GCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTG      <  SRR19973080.3254498/150‑1 (MQ=60)
                                                                                                                                                 GCACAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTG      <  SRR19973080.278613/150‑1 (MQ=60)
                                                                                                                                                    CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG   >  SRR19973080.2009381/1‑150 (MQ=60)
                                                                                                                                                    CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG   >  SRR19973080.4003646/1‑150 (MQ=60)
                                                                                                                                                    CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG   >  SRR19973080.4004788/1‑150 (MQ=60)
                                                                                                                                                    CAAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTG   >  SRR19973080.3026600/1‑150 (MQ=60)
                                                                                                                                                     AAGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGGTTGTTGT  >  SRR19973080.2806968/1‑150 (MQ=60)
                                                                                                                                                      |                                                                                                                                                    
CACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTACTGTCCGGACAACCATACTGCGACCTGGGGAATCAACTATACAAAAATATTGGTTTGTCTGTGGGCA‑TTTTTACTGTTCTTTATTATCATGCGCACACGATTAGCCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGT  >  CP009273/1865273‑1865570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑