Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 463,179 C→A 87.3% I104I (ATC→ATA)  cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273463,1790CA87.3% 527.0 / 71.5 219I104I (ATC→ATA) cofthiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (10/18);  new base A (84/107);  total (94/125)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCA  >  CP009273/463033‑463322
                                                                                                                                                  |                                                                                                                                               
   tCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCa                                                                                                                                             >  2:2070715/1‑148 (MQ=255)
   tCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCa                                                                                                                                             <  2:2557924/148‑1 (MQ=255)
    cGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCaa                                                                                                                                            <  1:2694325/148‑1 (MQ=255)
    cGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCaa                                                                                                                                            <  1:919692/148‑1 (MQ=255)
      cTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATg                                                                                                                                          >  2:909056/1‑148 (MQ=255)
      cTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATg                                                                                                                                          >  2:361385/1‑148 (MQ=255)
      cTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATg                                                                                                                                          <  2:2733877/148‑1 (MQ=255)
       tGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATga                                                                                                                                         <  2:1103602/148‑1 (MQ=255)
        ggATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgac                                                                                                                                        >  1:2961755/1‑148 (MQ=255)
         gATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacg                                                                                                                                       <  2:830041/148‑1 (MQ=255)
         gATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacg                                                                                                                                       >  1:1195751/1‑148 (MQ=255)
            gCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGacgacg                                                                                                                                    >  1:2031995/1‑148 (MQ=255)
             cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACgg                                                                                                                                   <  2:394682/148‑1 (MQ=255)
              gTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggt                                                                                                                                  <  1:1446375/148‑1 (MQ=255)
               tATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggtt                                                                                                                                 <  1:717587/148‑1 (MQ=255)
                aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                >  1:1723517/1‑148 (MQ=255)
                aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                <  1:3198986/148‑1 (MQ=255)
                aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                <  2:86906/148‑1 (MQ=255)
                aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                >  2:2825891/1‑148 (MQ=255)
                       tACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTAcc                                                                                                                         >  2:1401306/1‑148 (MQ=255)
                              aaCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAag                                                                                                                  <  2:1444753/148‑1 (MQ=255)
                               aCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAaga                                                                                                                 >  2:2810964/1‑148 (MQ=255)
                                  gAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGAt                                                                                                              >  1:533288/1‑148 (MQ=255)
                                  gAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGAt                                                                                                              >  2:2563609/1‑148 (MQ=255)
                                   aaCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATc                                                                                                             >  1:890514/1‑148 (MQ=255)
                                    tcgcgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATcc                                                                                                            <  1:347410/147‑1 (MQ=255)
                                    aCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATcc                                                                                                            <  2:1228134/148‑1 (MQ=255)
                                         cgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCg                                                                                                       <  1:404500/148‑1 (MQ=255)
                                         cgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCg                                                                                                       <  2:2031995/148‑1 (MQ=255)
                                             cATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgt                                                                                                   <  1:2652610/148‑1 (MQ=255)
                                             cATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATACAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgt                                                                                                   >  1:2041702/1‑148 (MQ=255)
                                              aTTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgtt                                                                                                  >  2:3003809/1‑148 (MQ=255)
                                                tctcTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGc                                                                                                >  2:1992112/1‑148 (MQ=255)
                                                tctcTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGc                                                                                                <  1:3147696/148‑1 (MQ=255)
                                                 ctctGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCa                                                                                               <  1:1396619/148‑1 (MQ=255)
                                                 ctctGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCa                                                                                               <  1:766762/148‑1 (MQ=255)
                                                  tctGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTAAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAg                                                                                              >  2:172849/1‑148 (MQ=255)
                                                     ggAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCa                                                                                           >  1:2771568/1‑148 (MQ=255)
                                                     ggAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCa                                                                                           >  2:2104095/1‑148 (MQ=255)
                                                     ggAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCa                                                                                           >  1:511231/1‑148 (MQ=255)
                                                       aaGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAtt                                                                                         <  1:1897457/148‑1 (MQ=255)
                                                         ggTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTg                                                                                       >  2:1500035/1‑148 (MQ=255)
                                                          gTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGt                                                                                      >  2:2382707/1‑148 (MQ=255)
                                                          gTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGt                                                                                      <  1:2430977/148‑1 (MQ=255)
                                                            gAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCt                                                                                    >  1:2183675/1‑148 (MQ=255)
                                                              aCTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCtat                                                                                  <  1:122374/148‑1 (MQ=255)
                                                              aCTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCtat                                                                                  <  1:122564/148‑1 (MQ=255)
                                                                ttttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAg                                                                                >  2:1782280/1‑148 (MQ=255)
                                                                ttttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAg                                                                                >  2:1780991/1‑148 (MQ=255)
                                                                    aCATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTTTATCAGCTATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGt                                                                            <  2:555825/148‑1 (MQ=255)
                                                                    aCATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGt                                                                            <  2:2306851/148‑1 (MQ=255)
                                                                       tCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGtttt                                                                         <  1:2628686/148‑1 (MQ=255)
                                                                          tgatgaTTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                      <  1:949837/148‑1 (MQ=255)
                                                                          tgatgaTTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                      <  1:2825891/148‑1 (MQ=255)
                                                                          tgatgaTTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGt                                                                      <  2:3020653/148‑1 (MQ=255)
                                                                            atgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTa                                                                    >  1:1280462/1‑148 (MQ=255)
                                                                                tttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAg                                                                <  2:2547268/148‑1 (MQ=255)
                                                                                 ttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGa                                                               >  2:1575329/1‑148 (MQ=255)
                                                                                 ttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGa                                                               >  2:1320224/1‑148 (MQ=255)
                                                                                   aCCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATa                                                             <  1:1666845/148‑1 (MQ=255)
                                                                                    ccTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                            >  1:1817426/1‑148 (MQ=255)
                                                                                    ccTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                            <  1:2196627/148‑1 (MQ=255)
                                                                                       gCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCg                                                         <  2:1234214/148‑1 (MQ=255)
                                                                                       gCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCg                                                         <  2:1467563/148‑1 (MQ=255)
                                                                                          gATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATg                                                      <  1:3254072/148‑1 (MQ=255)
                                                                                           aTGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGt                                                     >  1:2102739/1‑148 (MQ=255)
                                                                                                   gAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTaaaaaaaa                                             >  2:1427217/1‑148 (MQ=255)
                                                                                                   gAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaaaaaa                                             <  1:1059272/148‑1 (MQ=255)
                                                                                                    aGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAt                                            <  1:2563609/148‑1 (MQ=255)
                                                                                                        ggTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCa                                        <  2:1729517/148‑1 (MQ=255)
                                                                                                          tGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACt                                      <  2:3304027/148‑1 (MQ=255)
                                                                                                             tGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCgg                                   <  2:2902703/148‑1 (MQ=255)
                                                                                                             tGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCgg                                   <  1:961921/148‑1 (MQ=255)
                                                                                                             tGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCgg                                   <  2:1035933/148‑1 (MQ=255)
                                                                                                              gTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGc                                  >  2:1257753/1‑148 (MQ=255)
                                                                                                                          ggATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAg                      >  1:2519560/1‑148 (MQ=255)
                                                                                                                          ggATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAg                      >  1:2335894/1‑148 (MQ=255)
                                                                                                                          ggATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAg                      <  2:3183050/148‑1 (MQ=255)
                                                                                                                            aTACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGAt                    >  1:2550299/1‑148 (MQ=255)
                                                                                                                              aCCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCt                  >  2:1313042/1‑148 (MQ=255)
                                                                                                                                ccGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGTGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                <  1:1611405/148‑1 (MQ=255)
                                                                                                                                ccGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                >  2:2685284/1‑148 (MQ=255)
                                                                                                                                 cGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCt               >  2:2335658/1‑148 (MQ=255)
                                                                                                                                   aGCCAGCATGCATATCGTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTc             <  2:1890612/148‑1 (MQ=255)
                                                                                                                                   aGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTc             <  2:1723091/148‑1 (MQ=255)
                                                                                                                                    gCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCt            <  1:153024/148‑1 (MQ=255)
                                                                                                                                     ccAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtg           <  1:196459/148‑1 (MQ=255)
                                                                                                                                       aGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgtg         <  2:1197186/148‑1 (MQ=255)
                                                                                                                                       aGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgtg         >  2:3253994/1‑148 (MQ=255)
                                                                                                                                         catgcatATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGc       >  2:1953016/1‑148 (MQ=255)
                                                                                                                                          atgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCg      <  2:246111/148‑1 (MQ=255)
                                                                                                                                           tgcatATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGa     >  1:2355290/1‑148 (MQ=255)
                                                                                                                                           tgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGa     <  1:2899893/148‑1 (MQ=255)
                                                                                                                                            gcatATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATTTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAt    <  2:164100/148‑1 (MQ=255)
                                                                                                                                             catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc   <  2:234673/148‑1 (MQ=255)
                                                                                                                                             catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc   <  2:1668023/148‑1 (MQ=255)
                                                                                                                                             catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc   >  2:718417/1‑148 (MQ=255)
                                                                                                                                             catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc   >  1:230075/1‑148 (MQ=255)
                                                                                                                                             catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc   >  1:1165348/1‑148 (MQ=255)
                                                                                                                                              atatatTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCa  >  1:2892835/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCA  >  CP009273/463033‑463322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  >  CP009273/463032‑463327
                                                                                                                                                   |                                                                                                                                                    
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATT                                                                                                                                                    <  SRR19973070.2474733/150‑1 (MQ=60)
 CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTC                                                                                                                                                   <  SRR19973070.1909981/150‑1 (MQ=60)
 CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTC                                                                                                                                                   <  SRR19973070.2579161/150‑1 (MQ=60)
  TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCA                                                                                                                                                  <  SRR19973070.3084087/150‑1 (MQ=60)
     CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATG                                                                                                                                               <  SRR19973070.926244/150‑1 (MQ=60)
     CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATG                                                                                                                                               <  SRR19973070.926075/150‑1 (MQ=60)
     CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATG                                                                                                                                               <  SRR19973070.926022/150‑1 (MQ=60)
     CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATG                                                                                                                                               <  SRR19973070.2711777/150‑1 (MQ=60)
       CTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGAC                                                                                                                                             >  SRR19973070.496992/1‑150 (MQ=60)
       CTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGAC                                                                                                                                             >  SRR19973070.2980800/1‑150 (MQ=60)
        TGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACG                                                                                                                                            >  SRR19973070.1204160/1‑150 (MQ=60)
           ANGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACG                                                                                                                                         >  SRR19973070.2045744/1‑150 (MQ=60)
              CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                      <  SRR19973070.1453751/150‑1 (MQ=60)
               GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTG                                                                                                                                     >  SRR19973070.1735023/1‑150 (MQ=60)
               GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTG                                                                                                                                     >  SRR19973070.1735450/1‑150 (MQ=60)
               GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTG                                                                                                                                     <  SRR19973070.1456424/150‑1 (MQ=60)
               GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTG                                                                                                                                     <  SRR19973070.1824009/150‑1 (MQ=60)
                TATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGG                                                                                                                                    <  SRR19973070.722704/150‑1 (MQ=60)
                TATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGG                                                                                                                                    >  SRR19973070.3203646/1‑150 (MQ=60)
                 ATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGT                                                                                                                                   <  SRR19973070.3219828/150‑1 (MQ=60)
                 ATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGT                                                                                                                                   <  SRR19973070.3219689/150‑1 (MQ=60)
                      ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC                                                                                                                              <  SRR19973070.739485/150‑1 (MQ=60)
                      ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                              >  SRR19973070.2588312/1‑150 (MQ=60)
                         ACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGG                                                                                                                           >  SRR19973070.958037/1‑150 (MQ=60)
                               AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                     >  SRR19973070.693523/1‑150 (MQ=60)
                                 CGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGAT                                                                                                                   >  SRR19973070.536877/1‑150 (MQ=60)
                                  GGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATC                                                                                                                  >  SRR19973070.896802/1‑150 (MQ=60)
                                     TCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCT                                                                                                               <  SRR19973070.349936/150‑1 (MQ=60)
                                          CGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTT                                                                                                          <  SRR19973070.407968/150‑1 (MQ=60)
                                          CGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTT                                                                                                          <  SRR19973070.407472/150‑1 (MQ=60)
                                          CGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTT                                                                                                          <  SRR19973070.3071067/150‑1 (MQ=60)
                                           GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                         <  SRR19973070.781062/150‑1 (MQ=60)
                                           GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                         <  SRR19973070.2201752/150‑1 (MQ=60)
                                            TGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGT                                                                                                        <  SRR19973070.1479535/150‑1 (MQ=60)
                                            TGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATACAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGAGTTGT                                                                                                        >  SRR19973070.2055512/1‑150 (MQ=60)
                                              CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                      <  SRR19973070.2669869/150‑1 (MQ=60)
                                              CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                      <  SRR19973070.2670894/150‑1 (MQ=60)
                                                 TCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAG                                                                                                   <  SRR19973070.3168036/150‑1 (MQ=60)
                                                  CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGG                                                                                                  <  SRR19973070.1406027/150‑1 (MQ=60)
                                                  CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGG                                                                                                  <  SRR19973070.772145/150‑1 (MQ=60)
                                                  CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGG                                                                                                  >  SRR19973070.247801/1‑150 (MQ=60)
                                                    CTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCA                                                                                                >  SRR19973070.2789312/1‑150 (MQ=60)
                                                    CTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCA                                                                                                >  SRR19973070.514747/1‑150 (MQ=60)
                                                     TGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAT                                                                                               >  SRR19973070.2921476/1‑150 (MQ=60)
                                                       GAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTT                                                                                             <  SRR19973070.2565275/150‑1 (MQ=60)
                                                        AAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTG                                                                                            <  SRR19973070.1910310/150‑1 (MQ=60)
                                                         AGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGT                                                                                           <  SRR19973070.2179904/150‑1 (MQ=60)
                                                           GTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                         >  SRR19973070.1242854/1‑150 (MQ=60)
                                                           GTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                         >  SRR19973070.1242926/1‑150 (MQ=60)
                                                           GTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                         >  SRR19973070.2198117/1‑150 (MQ=60)
                                                           GTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                         <  SRR19973070.2446934/150‑1 (MQ=60)
                                                           GTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCT                                                                                         <  SRR19973070.2446935/150‑1 (MQ=60)
                                                              AACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATA                                                                                      <  SRR19973070.1371138/150‑1 (MQ=60)
                                                               ACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAG                                                                                     <  SRR19973070.123654/150‑1 (MQ=60)
                                                               ACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAG                                                                                     <  SRR19973070.123638/150‑1 (MQ=60)
                                                               ACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAG                                                                                     <  SRR19973070.123464/150‑1 (MQ=60)
                                                                CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGC                                                                                    <  SRR19973070.1918433/150‑1 (MQ=60)
                                                                 TTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCG                                                                                   <  SRR19973070.2557080/150‑1 (MQ=60)
                                                                      CATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTT                                                                              <  SRR19973070.2307442/150‑1 (MQ=60)
                                                                        TCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCG                                                                            <  SRR19973070.200748/150‑1 (MQ=60)
                                                                        TCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCG                                                                            <  SRR19973070.2645833/150‑1 (MQ=60)
                                                                        TCGTGATGATTGACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCG                                                                            <  SRR19973070.2645988/150‑1 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                         <  SRR19973070.956604/150‑1 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                         >  SRR19973070.1289407/1‑150 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                         <  SRR19973070.2844172/150‑1 (MQ=60)
                                                                           TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                         <  SRR19973070.2844178/150‑1 (MQ=60)
                                                                            GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTAT                                                                        <  SRR19973070.954084/150‑1 (MQ=60)
                                                                                   TACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAA                                                                 >  SRR19973070.1829951/1‑150 (MQ=60)
                                                                                    ACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAAT                                                                <  SRR19973070.1678293/150‑1 (MQ=60)
                                                                                     CCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATC                                                               <  SRR19973070.2353816/150‑1 (MQ=60)
                                                                                     CCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATC                                                               <  SRR19973070.2211122/150‑1 (MQ=60)
                                                                                      CTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCG                                                              >  SRR19973070.1662051/1‑150 (MQ=60)
                                                                                          GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                          >  SRR19973070.1215042/1‑150 (MQ=60)
                                                                                          GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                          >  SRR19973070.2116849/1‑150 (MQ=60)
                                                                                           GATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC                                                         <  SRR19973070.3275134/150‑1 (MQ=60)
                                                                                           GATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC                                                         <  SRR19973070.3275032/150‑1 (MQ=60)
                                                                                                    GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG                                                <  SRR19973070.1066706/150‑1 (MQ=60)
                                                                                                    GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG                                                <  SRR19973070.2729847/150‑1 (MQ=60)
                                                                                                     AGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGC                                               <  SRR19973070.2580350/150‑1 (MQ=60)
                                                                                                     AGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGC                                               <  SRR19973070.2580360/150‑1 (MQ=60)
                                                                                                       CTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCA                                             >  SRR19973070.205062/1‑150 (MQ=60)
                                                                                                           TGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG                                         >  SRR19973070.2014719/1‑150 (MQ=60)
                                                                                                              TGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCA                                      <  SRR19973070.968784/150‑1 (MQ=60)
                                                                                                                      CAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                              >  SRR19973070.1655332/1‑150 (MQ=60)
                                                                                                                         TGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAG                           >  SRR19973070.2535982/1‑150 (MQ=60)
                                                                                                                         TGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAG                           >  SRR19973070.2351397/1‑150 (MQ=60)
                                                                                                                           GGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGAT                         >  SRR19973070.2566979/1‑150 (MQ=60)
                                                                                                                           GGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGAT                         >  SRR19973070.2322377/1‑150 (MQ=60)
                                                                                                                                CCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT                    <  SRR19973070.2897915/150‑1 (MQ=60)
                                                                                                                                 CCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGTGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTT                   <  SRR19973070.1622634/150‑1 (MQ=60)
                                                                                                                                 CCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTT                   <  SRR19973070.1621931/150‑1 (MQ=60)
                                                                                                                                 CCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTT                   <  SRR19973070.2292496/150‑1 (MQ=60)
                                                                                                                                    AGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTG                <  SRR19973070.403274/150‑1 (MQ=60)
                                                                                                                                     GCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGT               <  SRR19973070.154306/150‑1 (MQ=60)
                                                                                                                                      CCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTG              <  SRR19973070.197960/150‑1 (MQ=60)
                                                                                                                                       CAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGG             <  SRR19973070.1856506/150‑1 (MQ=60)
                                                                                                                                        AGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGC            >  SRR19973070.52748/1‑150 (MQ=60)
                                                                                                                                         GCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCG           <  SRR19973070.2084662/150‑1 (MQ=60)
                                                                                                                                          CATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGA          >  SRR19973070.2370888/1‑150 (MQ=60)
                                                                                                                                           ATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAT         >  SRR19973070.278687/1‑150 (MQ=60)
                                                                                                                                            TGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATC        <  SRR19973070.2918654/150‑1 (MQ=60)
                                                                                                                                            TGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATC        >  SRR19973070.1173519/1‑150 (MQ=60)
                                                                                                                                            TGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATC        >  SRR19973070.1173510/1‑150 (MQ=60)
                                                                                                                                            TGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATC        >  SRR19973070.231701/1‑150 (MQ=60)
                                                                                                                                            TGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATC        <  SRR19973070.2918607/150‑1 (MQ=60)
                                                                                                                                             GCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCA       >  SRR19973070.2911555/1‑150 (MQ=60)
                                                                                                                                              CATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC      <  SRR19973070.1088528/150‑1 (MQ=60)
                                                                                                                                                  TATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  <  SRR19973070.3023011/150‑1 (MQ=60)
                                                                                                                                                  TATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGAAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  <  SRR19973070.915512/150‑1 (MQ=60)
                                                                                                                                                  TATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGAAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  <  SRR19973070.364031/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  >  CP009273/463032‑463327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑