Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,669,592 G→T 89.7% F342L (TTC→TTA)  pntB ← pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,669,5920GT89.7% 403.1 / 38.0 162F342L (TTC→TTA) pntBpyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (9/8);  new base T (82/63);  total (91/71)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGG  >  CP009273/1669447‑1669734
                                                                                                                                                 |                                                                                                                                              
tAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaa                                                                                                                                              <  2:56886/148‑1 (MQ=255)
 aGCACCAATCCCCAGTACGTTATCGTTATCACAAAAGACATATTTGATCTCGTCCATTACCAGCACGTTGTCATACGTTATTTTTGCTTCACCCAGAATTCCGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTaaa                                                                                                                                             <  1:902536/148‑1 (MQ=255)
  gCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAAc                                                                                                                                            >  1:1889680/1‑148 (MQ=255)
   cACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACg                                                                                                                                           >  2:2155216/1‑148 (MQ=255)
       aaTCACCAGTACGGTATCGGTATCAGAAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGcaca                                                                                                                                       <  1:1333671/148‑1 (MQ=255)
        aTCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAt                                                                                                                                      >  1:1933834/1‑148 (MQ=255)
         tCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAtt                                                                                                                                     >  2:3274608/1‑148 (MQ=255)
          cACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTa                                                                                                                                    >  1:2529574/1‑148 (MQ=255)
          cACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTa                                                                                                                                    <  2:3013759/148‑1 (MQ=255)
            ccAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAt                                                                                                                                  >  2:3205481/1‑148 (MQ=255)
            ccAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAt                                                                                                                                  >  2:3205554/1‑148 (MQ=255)
             cAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATa                                                                                                                                 >  1:2771880/1‑148 (MQ=255)
               gTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATAcc                                                                                                                               <  2:20677/148‑1 (MQ=255)
                 aCGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCAc                                                                                                                             >  1:1562177/1‑148 (MQ=255)
                 aCGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCAc                                                                                                                             >  1:1455304/1‑148 (MQ=255)
                 aCGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCAc                                                                                                                             >  1:1454617/1‑148 (MQ=255)
                          gtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCa                                                                                                                    <  1:1346823/148‑1 (MQ=255)
                           tatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCaa                                                                                                                   <  2:2941359/148‑1 (MQ=255)
                           tatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCaa                                                                                                                   >  1:3156524/1‑148 (MQ=255)
                           tatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCaa                                                                                                                   >  1:2765210/1‑148 (MQ=255)
                            atcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAt                                                                                                                  >  2:1948905/1‑148 (MQ=255)
                              cAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAAttt                                                                                                                >  1:3256453/1‑148 (MQ=255)
                                gCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtct                                                                                                              >  1:1063584/1‑148 (MQ=255)
                                     gTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                         <  2:1236803/148‑1 (MQ=255)
                                     gTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                         <  2:1236842/148‑1 (MQ=255)
                                     gTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTa                                                                                                         <  2:2742832/148‑1 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                        <  2:2155285/148‑1 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                        <  2:2669530/148‑1 (MQ=255)
                                          catTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTc                                                                                                    >  1:2542230/1‑148 (MQ=255)
                                            ttGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                  >  1:2973805/1‑148 (MQ=255)
                                             tGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGc                                                                                                 >  1:1973319/1‑148 (MQ=255)
                                             tGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGc                                                                                                 >  1:1973292/1‑148 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                               >  1:3308821/1‑148 (MQ=255)
                                                tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                              >  1:2131009/1‑148 (MQ=255)
                                                 ctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                             >  2:717339/1‑148 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                           >  1:2342295/1‑148 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                           >  2:428623/1‑148 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                           >  2:428827/1‑148 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTAATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                           <  2:2047972/148‑1 (MQ=255)
                                                   cGTCCATTGCCAGCACTATGTCAAACGGTACTTTTGCTCCAGCCAGCAATCCGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTGCAGCGACAgg                                                                                           <  2:1970106/148‑1 (MQ=255)
                                                     tCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGat                                                                                         >  1:44219/1‑148 (MQ=255)
                                                      ccttttCCAGCACGTTGTCATACGTTACTTTTGCTTCAGCAAGAAATAGGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGata                                                                                        <  1:2403505/145‑1 (MQ=255)
                                                      ccATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGata                                                                                        >  2:2327482/1‑148 (MQ=255)
                                                      ccATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGata                                                                                        >  1:3083570/1‑148 (MQ=255)
                                                      caaTTTCAAGAACGATTTAATACGTTACTTTTGATTCAGCCTGCAATACGTTATTTTGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGAACATTAATACCACGAGCGCGCATTTTCTCAGTAATTTCAGCGACAGGata                                                                                        <  1:2402526/146‑1 (MQ=255)
                                                                cACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAGTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgc                                                                              >  2:2598432/1‑148 (MQ=255)
                                                                 aCGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcg                                                                             >  2:1584489/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                            >  1:2784751/1‑148 (MQ=255)
                                                                      gTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                        >  1:1090235/1‑148 (MQ=255)
                                                                      gTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACt                                                                        >  2:574759/1‑148 (MQ=255)
                                                                       tCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTg                                                                       <  1:2491781/148‑1 (MQ=255)
                                                                         aTACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGcc                                                                     <  2:3034837/148‑1 (MQ=255)
                                                                          tACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCa                                                                    <  2:2577996/148‑1 (MQ=255)
                                                                          tACGGTACTTTTGCTTCAGCCAGCAATACGTTCATAGGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCa                                                                    <  2:1512463/148‑1 (MQ=255)
                                                                            ggttACTTTTGTTTCAGCCACAATTAGTTTCATTTGACCAGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                  <  1:403232/145‑1 (MQ=255)
                                                                            cGGTATTTTTGCTTCCGCCAGAAATCGGTTCTTCTGTCCAGTCAAACGCCCCGCGACCGGGTGGATACCTAACCGCACATTAATCCCACGAGCGCGCATTTTCTCAGTATTTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                  <  1:2703452/148‑1 (MQ=255)
                                                                            cGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                  <  2:69909/148‑1 (MQ=255)
                                                                                  ttttGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAc                                                            >  2:2183989/1‑148 (MQ=255)
                                                                                  ttttGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAc                                                            >  2:2182882/1‑148 (MQ=255)
                                                                                   tttGATTCCCCCACCAATACGTTCATAGGTCCAGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAcc                                                           <  1:47080/148‑1 (MQ=255)
                                                                                    ttGCTCCACCCACAAATACTTTCATATGTCCCGCCAAACGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGCGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                          <  1:54113/148‑1 (MQ=255)
                                                                                         tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                     >  2:2897429/1‑148 (MQ=255)
                                                                                           acccagaAAGACTTTCATCGGACCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTa                                                   <  1:2557236/146‑1 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                  <  1:3265463/148‑1 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                  >  2:1399665/1‑148 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                  >  2:852786/1‑148 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                  >  2:2364128/1‑148 (MQ=255)
                                                                                                gcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACATAAACGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAt                                              <  2:263435/148‑1 (MQ=255)
                                                                                                 cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTCCCCCGGAGTAATGATc                                             >  2:2970164/1‑148 (MQ=255)
                                                                                                 cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTCATGATc                                             >  1:298989/1‑148 (MQ=255)
                                                                                                 cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTACTGATc                                             >  2:2725149/1‑148 (MQ=255)
                                                                                                 cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATc                                             >  1:3039578/1‑148 (MQ=255)
                                                                                                 cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATc                                             >  2:1073714/1‑148 (MQ=255)
                                                                                                   aTACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                           >  1:1654435/1‑148 (MQ=255)
                                                                                                      gtttaatatGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGa                                        <  1:1251740/143‑1 (MQ=255)
                                                                                                      gtttCATAGGGCCTGGCAAACGCCCCGCGCCCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGa                                        <  1:1729817/146‑1 (MQ=255)
                                                                                                       gTTCTTATGTCCAGCAAAACTCCCCGCGACCGGGTGGATACCTAAAGGGACATTAATACCACGAGCGCGCAATTTATCAGTAATTTCAGCGACAGGATATTGCGCCTTCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGaa                                       <  2:367852/148‑1 (MQ=255)
                                                                                                       gTTAATATGGCCAGGCAAACGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGGGCGCATTTTCTCATTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGaa                                       <  2:367787/148‑1 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                     <  2:2748853/148‑1 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                     >  2:2619420/1‑148 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                     >  2:2959929/1‑148 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                     <  2:379877/148‑1 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                     >  2:3084077/1‑148 (MQ=255)
                                                                                                          cATATGTCCAGGCAAACGCCCCGCTACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAGTGCGCCTGCGCGACTGCCAGGCCGTACCCCGGAGTAATGATCACTGAATgg                                    >  1:2315643/1‑148 (MQ=255)
                                                                                                              tGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGt                                <  2:1294998/148‑1 (MQ=255)
                                                                                                               gTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                               <  2:1063584/148‑1 (MQ=255)
                                                                                                               gTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                               >  1:2267897/1‑148 (MQ=255)
                                                                                                               gTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                               >  2:1850370/1‑148 (MQ=255)
                                                                                                               gTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                               >  2:1379317/1‑148 (MQ=255)
                                                                                                                tCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                              <  1:683371/148‑1 (MQ=255)
                                                                                                                accAGCCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTGCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                              <  1:683213/147‑1 (MQ=255)
                                                                                                                     gCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcag                         >  1:239320/1‑148 (MQ=255)
                                                                                                                         aCGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                     <  1:42227/148‑1 (MQ=255)
                                                                                                                             cccGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCg                 <  2:1541635/148‑1 (MQ=255)
                                                                                                                               cgcgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCt               >  1:1071857/1‑148 (MQ=255)
                                                                                                                                 cgACCGGGTGGATACCTAAACGCACATTAATCCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGt             <  2:857092/148‑1 (MQ=255)
                                                                                                                                 cgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGt             <  1:2151924/148‑1 (MQ=255)
                                                                                                                                       ggTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtcttc       <  1:1235844/148‑1 (MQ=255)
                                                                                                                                          ggATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGc    >  1:2804462/1‑148 (MQ=255)
                                                                                                                                            aTACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCgg  >  1:1946596/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGG  >  CP009273/1669447‑1669734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  CP009273/1669444‑1669741
                                                                                                                                                    |                                                                                                                                                     
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTA                                                                                                                                                      >  SRR19973070.2766515/1‑150 (MQ=60)
 GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAA                                                                                                                                                     >  SRR19973070.2920966/1‑150 (MQ=60)
 GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAA                                                                                                                                                     >  SRR19973070.1253010/1‑150 (MQ=60)
   TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAAC                                                                                                                                                   >  SRR19973070.1902500/1‑150 (MQ=60)
   TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAAC                                                                                                                                                   >  SRR19973070.1902603/1‑150 (MQ=60)
    AGCACCAATCCCCAGTACGTTATCGTTATCACAAAAGACATATTTGATCTCGTCCATTACCAGCACGTTGTCATACGTTATTTTTGCTTCACCCAGAATTCCGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACG                                                                                                                                                  <  SRR19973070.908937/150‑1 (MQ=53)
         CAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAT                                                                                                                                             >  SRR19973070.1946843/1‑150 (MQ=60)
          AATCACCAGTACGGTATCGGTATCAGAAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATT                                                                                                                                            <  SRR19973070.1342857/150‑1 (MQ=60)
           ATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTA                                                                                                                                           >  SRR19973070.2546099/1‑150 (MQ=60)
              acctgtcggttatcgttatcagaaacttaatatttgatctcgtcaattcccagcacgttgtattccgttatttttgattcagccagaaatccgttaattgggcaagcaAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATA                                                                                                                                        <  SRR19973070.284912/42‑1 (MQ=60)
              ACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATA                                                                                                                                        >  SRR19973070.2789594/1‑150 (MQ=60)
              ACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATA                                                                                                                                        >  SRR19973070.2063239/1‑150 (MQ=60)
              ACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATA                                                                                                                                        >  SRR19973070.2789627/1‑150 (MQ=60)
                  GTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCAC                                                                                                                                    >  SRR19973070.1464752/1‑150 (MQ=60)
                  GTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCAC                                                                                                                                    >  SRR19973070.1465446/1‑150 (MQ=60)
                  GTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCAC                                                                                                                                    >  SRR19973070.1573169/1‑150 (MQ=60)
                       TTATCGTTATCAGAAAAGTAATCATTGATCTCGTCCATTTCCAGCACGTTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATTTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCG                                                                                                                               <  SRR19973070.1555502/150‑1 (MQ=60)
                            GGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAA                                                                                                                          >  SRR19973070.2782922/1‑150 (MQ=60)
                            GGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAA                                                                                                                          >  SRR19973070.3176928/1‑150 (MQ=60)
                             GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGTTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAT                                                                                                                         <  SRR19973070.1096311/150‑1 (MQ=60)
                             GTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAT                                                                                                                         <  SRR19973070.1356065/150‑1 (MQ=60)
                               ATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTT                                                                                                                       >  SRR19973070.3277427/1‑150 (MQ=60)
                                TCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTC                                                                                                                      >  SRR19973070.234442/1‑150 (MQ=60)
                                 CAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCT                                                                                                                     >  SRR19973070.1071042/1‑150 (MQ=60)
                                 CAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCT                                                                                                                     >  SRR19973070.198481/1‑150 (MQ=60)
                                 CAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCT                                                                                                                     >  SRR19973070.2733352/1‑150 (MQ=60)
                                     AAAGTCATCTTTGATCTCGTCCTTTTCCAGCACGTTGTCATACGGTACTTTTGCTTCAGCCAGAAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGT                                                                                                                 <  SRR19973070.1290489/150‑1 (MQ=60)
                                      AAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTA                                                                                                                >  SRR19973070.1656611/1‑150 (MQ=60)
                                        GTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAATCGCTCAGTAATACCACGAGCGCGCgcgtgctcagtagt                                                                                                              >  SRR19973070.1899374/1‑136 (MQ=60)
                                        GTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAT                                                                                                              >  SRR19973070.2305626/1‑150 (MQ=60)
                                          CATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTT                                                                                                            >  SRR19973070.2149495/1‑150 (MQ=60)
                                           ATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTC                                                                                                           >  SRR19973070.2558854/1‑150 (MQ=60)
                                             CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                         >  SRR19973070.2992901/1‑150 (MQ=60)
                                              ATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGC                                                                                                        >  SRR19973070.1986633/1‑150 (MQ=60)
                                              ATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGC                                                                                                        >  SRR19973070.1986661/1‑150 (MQ=60)
                                                TGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGA                                                                                                      >  SRR19973070.3329994/1‑150 (MQ=60)
                                                 GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                     >  SRR19973070.361575/1‑150 (MQ=60)
                                                 GATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAC                                                                                                     >  SRR19973070.2145257/1‑150 (MQ=60)
                                                  ATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACA                                                                                                    >  SRR19973070.3232189/1‑150 (MQ=60)
                                                  ATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACA                                                                                                    >  SRR19973070.2535358/1‑150 (MQ=60)
                                                   tctcgtcatttaccagcactttgaataacgttatttttgttacagcaataattccgttaatttgtcctggaAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAG                                                                                                   <  SRR19973070.878037/79‑1 (MQ=60)
                                                    CTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGG                                                                                                  >  SRR19973070.2357834/1‑150 (MQ=60)
                                                      CGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGAT                                                                                                >  SRR19973070.44781/1‑150 (MQ=60)
                                                       GTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATA                                                                                               >  SRR19973070.3103397/1‑150 (MQ=60)
                                                         caatttcaagaacgatttaATACGTTACTTTTGATTCAGCCTGCAATACGTTATTTTGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGAACATTAATACCACGAGCGCGCATTTTCTCAGTAATTTCAGCGACAGGATATT                                                                                             <  SRR19973070.2418371/131‑1 (MQ=60)
                                                         CCTTTTCCAGCACGTTGTCATACGTTACTTTTGCTTCAGCAAGAAATAGGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                             <  SRR19973070.2419355/150‑1 (MQ=60)
                                                               cacccagtttgtaatcgggtattttggcttcagccccaaataggttaatatgccctggaaaaccCCCCGCGACCGGGTGGATACCTAAACCCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCT                                                                                       <  SRR19973070.2028526/86‑1 (MQ=60)
                                                                cagaactttgtaatacgttaattttgattcagccacaaatagtttaatttgccaaggaaacccCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTG                                                                                      <  SRR19973070.350099/87‑1 (MQ=60)
                                                                   CACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGC                                                                                   >  SRR19973070.2805440/1‑150 (MQ=60)
                                                                   CACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGC                                                                                   >  SRR19973070.2802666/1‑150 (MQ=60)
                                                                       ATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACT                                                                               >  SRR19973070.1097769/1‑150 (MQ=60)
                                                                         ttcacagtgtactttggcttcccccagcaatactttcttttgccccggaaacccCCCCGCGACCGGGTGTATACCAAAACGCAAATTAATACCACGAGCGCGCAATTTCTCAGTATTTTCAGCGACAGGATATTGCGCCTGCGCGACTGC                                                                             <  SRR19973070.1191910/96‑1 (MQ=60)
                                                                         ttaatagttttcttttgatacccccccaaatacgtacaattgtcccgcaAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCCCGAGCGCGCATTTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGC                                                                             <  SRR19973070.1192897/101‑1 (MQ=60)
                                                                         taaataggttaattttgattcagcaacaaatcggttaattggccccgcaAAACGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCTCAGTATTTTCAGCGACAGGATATTGCGCCTGCGCGACTGC                                                                             <  SRR19973070.1193109/101‑1 (MQ=60)
                                                                          TCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCC                                                                            <  SRR19973070.2507949/150‑1 (MQ=60)
                                                                           aataggttatttttgcttcccccacCAATACGTTCATATGTCCAGCCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCA                                                                           <  SRR19973070.1608054/125‑1 (MQ=60)
                                                                           aaaccggaacttttgattcccccacaaacccttacatttcacctgcaAAACGCCCCGCGACCGGGTGTATACCGAAACGCAAATTAAAACCACGCGCGCGCAATTTCCCTGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCA                                                                           <  SRR19973070.677938/103‑1 (MQ=60)
                                                                             TACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATG                                                                         <  SRR19973070.3045993/150‑1 (MQ=60)
                                                                               ggttacttttgtttcagccacaattagtttcatttgaccagcaAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCC                                                                       <  SRR19973070.406193/107‑1 (MQ=60)
                                                                               CGGTATTTTTGCTTCCGCCAGAAATCGGTTCTTCTGTCCAGTCAAACGCCCCGCGACCGGGTGGATACCTAACCGCACATTAATCCCACGAGCGCGCATTTTCTCAGTATTTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCC                                                                       <  SRR19973070.2720940/150‑1 (MQ=60)
                                                                                   ACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAC                                                                   >  SRR19973070.3154369/1‑150 (MQ=60)
                                                                                      tttgattcccccacCAATACGTTCATAGGTCCAGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCC                                                                <  SRR19973070.47668/136‑1 (MQ=60)
                                                                                       ttgctccacccacaAATACTTTCATATGTCCCGCCAAACGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGCGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCG                                                               <  SRR19973070.54753/136‑1 (MQ=60)
                                                                                       ttccgacacccaccaatccgttcaaaggcCCAGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACATTCATACCACCAGCGCGCAATTTCACAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCG                                                               <  SRR19973070.54754/121‑1 (MQ=60)
                                                                                              acccagaaatactttatttTGTCCAGGAAACCGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        <  SRR19973070.2570216/131‑1 (MQ=60)
                                                                                              acccagaaagactttcatcggaCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        <  SRR19973070.2573944/128‑1 (MQ=60)
                                                                                              AGCCAGCAATAGGTTCATATGTCCAGGAAACCGCCCCGCGACCGGGTGGATACCTAACCGCACATTAATCCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        <  SRR19973070.1753564/150‑1 (MQ=60)
                                                                                               GCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATG                                                       <  SRR19973070.3286478/150‑1 (MQ=60)
                                                                                                  AGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTCATGATC                                                    >  SRR19973070.301071/1‑150 (MQ=60)
                                                                                                  AGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATC                                                    >  SRR19973070.3058903/1‑150 (MQ=60)
                                                                                                    CAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  >  SRR19973070.3312188/1‑150 (MQ=60)
                                                                                                    CAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  >  SRR19973070.1665841/1‑150 (MQ=60)
                                                                                                         gtttcatagggcctGGCAAACGCCCCGCGCCCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAAT                                             <  SRR19973070.1741812/136‑1 (MQ=60)
                                                                                                         gtttaATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAAT                                             <  SRR19973070.1260523/145‑1 (MQ=60)
                                                                                                         CGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAAT                                             >  SRR19973070.2439655/1‑150 (MQ=60)
                                                                                                         CGTTCATATGACCAGGAAAACCCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCCCGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAAT                                             <  SRR19973070.57216/150‑1 (MQ=60)
                                                                                                           TTCATATGTCCAGGCAAACGCCCCGCTACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAGTGCGCCTGCGCGACTGCCAGGCCGTACCCCGGAGTAATGATCACTGAATGG                                           >  SRR19973070.2331030/1‑150 (MQ=60)
                                                                                                            acatagttcatGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACTTTAATACCACGAGCGCGCATTTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGG                                          <  SRR19973070.1405880/139‑1 (MQ=60)
                                                                                                                ATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTT                                      <  SRR19973070.1386236/150‑1 (MQ=60)
                                                                                                                ATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTT                                      >  SRR19973070.2282956/1‑150 (MQ=60)
                                                                                                                   accagccaacCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTGCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTT                                   <  SRR19973070.688082/140‑1 (MQ=60)
                                                                                                                   TCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTT                                   <  SRR19973070.688241/150‑1 (MQ=60)
                                                                                                                     CAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                 >  SRR19973070.521818/1‑150 (MQ=60)
                                                                                                                      AGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAG                                >  SRR19973070.241027/1‑150 (MQ=60)
                                                                                                                      AGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAG                                >  SRR19973070.240978/1‑150 (MQ=60)
                                                                                                                            ACGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTC                          <  SRR19973070.42762/150‑1 (MQ=60)
                                                                                                                            ACCCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTC                          <  SRR19973070.43793/150‑1 (MQ=60)
                                                                                                                                CCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCT                      >  SRR19973070.1079345/1‑150 (MQ=60)
                                                                                                                                    CGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCT                  <  SRR19973070.2166243/150‑1 (MQ=60)
                                                                                                                                          GGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTG            <  SRR19973070.1244492/150‑1 (MQ=60)
                                                                                                                                           GTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGC           >  SRR19973070.2822581/1‑150 (MQ=60)
                                                                                                                                             GGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGG         >  SRR19973070.1959669/1‑150 (MQ=60)
                                                                                                                                                  CCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATT    >  SRR19973070.2410259/1‑150 (MQ=60)
                                                                                                                                                    TAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  SRR19973070.1349324/1‑150 (MQ=60)
                                                                                                                                                    TAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  SRR19973070.1349537/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  CP009273/1669444‑1669741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑