Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,689,180 T→G 92.0% E383D (GAA→GAC)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,689,1800TG92.0% 686.0 / 23.9 187E383D (GAA→GAC) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (6/9);  new base G (78/94);  total (84/103)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 189 total aligned reads displayed.

GTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCT  >  CP009273/1689034‑1689324
                                                                                                                                                  |                                                                                                                                                
gTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGttt                                                                                                                                                 <  2:1119355/148‑1 (MQ=255)
  tGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGGTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTcc                                                                                                                                               <  2:2933452/148‑1 (MQ=255)
     ttCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCg                                                                                                                                            <  1:2777627/148‑1 (MQ=255)
     ttCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCg                                                                                                                                            <  2:374474/148‑1 (MQ=255)
       ccGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGtt                                                                                                                                          <  2:1243746/148‑1 (MQ=255)
        cGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTg                                                                                                                                         <  1:3206618/148‑1 (MQ=255)
        cGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTg                                                                                                                                         <  1:2141310/148‑1 (MQ=255)
         gCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGa                                                                                                                                        <  2:2577687/148‑1 (MQ=255)
          ccAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGAc                                                                                                                                       >  1:2104777/1‑148 (MQ=255)
             gTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGc                                                                                                                                    <  2:91585/148‑1 (MQ=255)
              tGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGcc                                                                                                                                   >  1:1990116/1‑148 (MQ=255)
               ggCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCct                                                                                                                                  <  1:739999/148‑1 (MQ=255)
               ggCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCct                                                                                                                                  <  1:3046577/148‑1 (MQ=255)
                 cgcgAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCctct                                                                                                                                <  2:3282107/148‑1 (MQ=255)
                    gAAATATTCCCGTGCACCGTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCg                                                                                                                             <  2:1567816/148‑1 (MQ=255)
                      aaTATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCt                                                                                                                           <  1:2675649/148‑1 (MQ=255)
                       atatTCCCGTGCACCTTGCGGACGGGTATCCGGGTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTg                                                                                                                          >  2:397465/1‑148 (MQ=255)
                        tatTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGt                                                                                                                         >  1:3247996/1‑148 (MQ=255)
                           tCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACa                                                                                                                      >  1:1900388/1‑148 (MQ=255)
                           tCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACa                                                                                                                      >  1:230368/1‑148 (MQ=255)
                            cccGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAg                                                                                                                     >  2:2853754/1‑148 (MQ=255)
                             ccGTGCACCTTTCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCAGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGt                                                                                                                    >  1:1366240/1‑148 (MQ=255)
                              cGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGtt                                                                                                                   <  1:790127/148‑1 (MQ=255)
                                tGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCt                                                                                                                 <  1:886240/148‑1 (MQ=255)
                                tGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCt                                                                                                                 >  1:653278/1‑148 (MQ=255)
                                tGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCt                                                                                                                 <  1:1590708/148‑1 (MQ=255)
                                 gCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCtt                                                                                                                >  1:298692/1‑148 (MQ=255)
                                   aCCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCTCGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCTCTGTACAGTTCTTTc                                                                                                              <  2:2723119/148‑1 (MQ=255)
                                     cTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCgg                                                                                                            >  2:2896338/1‑148 (MQ=255)
                                      ttGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGc                                                                                                           <  1:450585/148‑1 (MQ=255)
                                      ttGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGc                                                                                                           <  2:1295123/148‑1 (MQ=255)
                                      ttGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGc                                                                                                           <  1:1214532/148‑1 (MQ=255)
                                       tGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCt                                                                                                          <  1:2971355/148‑1 (MQ=255)
                                        gCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCtt                                                                                                         <  2:1381054/148‑1 (MQ=255)
                                        gCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCtt                                                                                                         <  1:1725091/148‑1 (MQ=255)
                                         cGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttg                                                                                                        <  1:1119111/148‑1 (MQ=255)
                                           gACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttgtt                                                                                                      <  1:869073/148‑1 (MQ=255)
                                            aCGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttgttg                                                                                                     <  2:2437079/148‑1 (MQ=255)
                                                   tCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCtt                                                                                              <  1:2846152/148‑1 (MQ=255)
                                                   tCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCtt                                                                                              >  2:2378067/1‑148 (MQ=255)
                                                   tCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCtt                                                                                              >  2:2378065/1‑148 (MQ=255)
                                                        ttCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGaaa                                                                                         >  2:599313/1‑148 (MQ=255)
                                                         tCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAc                                                                                        >  1:2456354/1‑148 (MQ=255)
                                                            ttGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCaa                                                                                     <  2:2619976/148‑1 (MQ=255)
                                                            ttGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCaa                                                                                     >  2:2276653/1‑148 (MQ=255)
                                                             tGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAAt                                                                                    >  2:2886434/1‑148 (MQ=255)
                                                              ggCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATg                                                                                   <  1:370896/148‑1 (MQ=255)
                                                                cAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGcc                                                                                 <  2:1215903/148‑1 (MQ=255)
                                                                   tACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaa                                                                              >  1:1269042/1‑148 (MQ=255)
                                                                   tACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaa                                                                              <  1:1917022/148‑1 (MQ=255)
                                                                       ccACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagag                                                                          <  1:2464491/148‑1 (MQ=255)
                                                                       ccACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagag                                                                          <  2:1448689/148‑1 (MQ=255)
                                                                        cacaTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                         <  2:2120043/148‑1 (MQ=255)
                                                                        cacaTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                         >  2:3095832/1‑148 (MQ=255)
                                                                        cacaTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                         <  2:644894/148‑1 (MQ=255)
                                                                          cctCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAgg                                                                       <  2:500929/146‑1 (MQ=255)
                                                                           aTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGt                                                                      >  2:731186/1‑148 (MQ=255)
                                                                            tCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGtt                                                                     >  2:1670093/1‑148 (MQ=255)
                                                                               ccacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTaaa                                                                  >  2:2054351/1‑148 (MQ=255)
                                                                               ccacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTaaa                                                                  >  1:949775/1‑148 (MQ=255)
                                                                                 acGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGc                                                                <  1:2876769/148‑1 (MQ=255)
                                                                                  cGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGcc                                                               >  1:2808423/1‑148 (MQ=255)
                                                                                    cTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGa                                                             <  1:2121592/148‑1 (MQ=255)
                                                                                     ttGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAc                                                            <  1:720233/148‑1 (MQ=255)
                                                                                        ggtggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagc                                                         >  1:270031/1‑148 (MQ=255)
                                                                                        ggtggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagc                                                         >  2:707057/1‑148 (MQ=255)
                                                                                        ggtggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagc                                                         <  1:2559387/148‑1 (MQ=255)
                                                                                          tggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcag                                                       >  2:3199819/1‑148 (MQ=255)
                                                                                          tggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcag                                                       >  1:2249710/1‑148 (MQ=255)
                                                                                           ggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagc                                                      <  2:1225617/148‑1 (MQ=255)
                                                                                            gtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagca                                                     <  1:887729/148‑1 (MQ=255)
                                                                                            gtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagca                                                     <  1:3098672/148‑1 (MQ=255)
                                                                                            gtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagca                                                     <  1:3011234/148‑1 (MQ=255)
                                                                                             tttttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagcagcag                                                    <  1:29553/148‑1 (MQ=255)
                                                                                              ttttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGt                                                   >  2:1153888/1‑148 (MQ=255)
                                                                                                     aCGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtcatca                                            >  2:1703854/1‑148 (MQ=255)
                                                                                                       gcgcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCatcaat                                          <  1:2523434/148‑1 (MQ=255)
                                                                                                        cgcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCatcaatc                                         <  2:2146789/148‑1 (MQ=255)
                                                                                                        cgcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCatcaatc                                         <  2:2506454/148‑1 (MQ=255)
                                                                                                         gcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCatcaatca                                        <  2:2802011/148‑1 (MQ=255)
                                                                                                         gcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCatcaatca                                        <  2:3263854/148‑1 (MQ=255)
                                                                                                          cTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcac                                       >  1:1121001/1‑148 (MQ=255)
                                                                                                             tCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                    >  2:379173/1‑148 (MQ=255)
                                                                                                              cAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACg                                   <  1:2647815/148‑1 (MQ=255)
                                                                                                               aGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGa                                  <  1:519774/148‑1 (MQ=255)
                                                                                                                 ctctTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATg                                >  2:1701413/1‑148 (MQ=255)
                                                                                                                   ctTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGcc                              >  2:1821068/1‑148 (MQ=255)
                                                                                                                   ctTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGcc                              <  1:276149/148‑1 (MQ=255)
                                                                                                                     ttAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCAt                            <  1:2587207/148‑1 (MQ=255)
                                                                                                                     ttAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCAt                            >  2:850188/1‑148 (MQ=255)
                                                                                                                       aaTCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGt                          >  1:1935940/1‑148 (MQ=255)
                                                                                                                         tCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTc                        >  2:2993567/1‑148 (MQ=255)
                                                                                                                           gCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCAt                      <  1:402523/148‑1 (MQ=255)
                                                                                                                              tGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTg                   <  2:3191784/148‑1 (MQ=255)
                                                                                                                               gTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGc                  >  1:644338/1‑148 (MQ=255)
                                                                                                                                tAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGcc                 >  1:3080484/1‑148 (MQ=255)
                                                                                                                                    tGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGt             >  1:906766/1‑148 (MQ=255)
                                                                                                                                    tGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGt             >  2:187714/1‑148 (MQ=255)
                                                                                                                                         ttGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGc        <  1:1486116/148‑1 (MQ=255)
                                                                                                                                             tGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAtct    >  1:1766843/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCT  >  CP009273/1689034‑1689324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCAG  >  CP009273/1689031‑1689328
                                                                                                                                                     |                                                                                                                                                    
CGCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTG                                                                                                                                                      >  SRR19973070.1833027/1‑150 (MQ=60)
   GTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCC                                                                                                                                                   <  SRR19973070.353457/150‑1 (MQ=60)
   GTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCC                                                                                                                                                   <  SRR19973070.2030995/150‑1 (MQ=60)
      GCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCG                                                                                                                                                <  SRR19973070.988726/150‑1 (MQ=60)
        TTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTT                                                                                                                                              <  SRR19973070.2795473/150‑1 (MQ=60)
           CGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGAC                                                                                                                                           >  SRR19973070.2118900/1‑150 (MQ=60)
           CGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGAC                                                                                                                                           >  SRR19973070.443260/1‑150 (MQ=60)
           CGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGAC                                                                                                                                           <  SRR19973070.2155585/150‑1 (MQ=60)
           CGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGAC                                                                                                                                           <  SRR19973070.3227359/150‑1 (MQ=60)
               AGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCC                                                                                                                                       >  SRR19973070.2003592/1‑150 (MQ=60)
               AGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCC                                                                                                                                       >  SRR19973070.2150404/1‑150 (MQ=60)
               AGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCC                                                                                                                                       >  SRR19973070.893190/1‑150 (MQ=60)
               AGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCC                                                                                                                                       >  SRR19973070.2559398/1‑150 (MQ=60)
                GTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCT                                                                                                                                      <  SRR19973070.382968/150‑1 (MQ=60)
                  GGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCT                                                                                                                                    <  SRR19973070.745234/150‑1 (MQ=60)
                  GGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCT                                                                                                                                    <  SRR19973070.3065966/150‑1 (MQ=60)
                  GGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCT                                                                                                                                    <  SRR19973070.3065952/150‑1 (MQ=60)
                      CGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGC                                                                                                                                >  SRR19973070.1259889/1‑150 (MQ=60)
                         AATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGT                                                                                                                             >  SRR19973070.3268926/1‑150 (MQ=60)
                         AATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGT                                                                                                                             <  SRR19973070.2693021/150‑1 (MQ=60)
                            ATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACA                                                                                                                          >  SRR19973070.1747713/1‑150 (MQ=60)
                            ATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACA                                                                                                                          >  SRR19973070.231996/1‑150 (MQ=60)
                            ATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACA                                                                                                                          >  SRR19973070.1913254/1‑150 (MQ=60)
                              TCCCGTGCACCTTTCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCAGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGT                                                                                                                        >  SRR19973070.1375544/1‑150 (MQ=60)
                              TCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGT                                                                                                                        <  SRR19973070.3042187/150‑1 (MQ=60)
                                 CGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCT                                                                                                                     >  SRR19973070.657937/1‑150 (MQ=60)
                                 CGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCT                                                                                                                     <  SRR19973070.795605/150‑1 (MQ=60)
                                 CGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCT                                                                                                                     >  SRR19973070.2332395/1‑150 (MQ=60)
                                  GTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTT                                                                                                                    >  SRR19973070.1462524/1‑150 (MQ=60)
                                  GTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTT                                                                                                                    >  SRR19973070.3221134/1‑150 (MQ=60)
                                  GTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTT                                                                                                                    >  SRR19973070.300772/1‑150 (MQ=60)
                                   TGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTT                                                                                                                   <  SRR19973070.892498/150‑1 (MQ=60)
                                   TGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTT                                                                                                                   <  SRR19973070.1601834/150‑1 (MQ=60)
                                   TGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTT                                                                                                                   <  SRR19973070.3249725/150‑1 (MQ=60)
                                   TGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTT                                                                                                                   <  SRR19973070.892496/150‑1 (MQ=60)
                                         TTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTT                                                                                                             <  SRR19973070.1223053/150‑1 (MQ=60)
                                         TTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTT                                                                                                             <  SRR19973070.2882367/150‑1 (MQ=60)
                                         TTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTT                                                                                                             <  SRR19973070.453832/150‑1 (MQ=60)
                                          TGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTG                                                                                                            <  SRR19973070.2990441/150‑1 (MQ=60)
                                          TGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTG                                                                                                            <  SRR19973070.2990460/150‑1 (MQ=60)
                                          TGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTG                                                                                                            <  SRR19973070.656241/150‑1 (MQ=60)
                                           GCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGT                                                                                                           <  SRR19973070.1737034/150‑1 (MQ=60)
                                            CGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTT                                                                                                          <  SRR19973070.1126756/150‑1 (MQ=60)
                                             GGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTG                                                                                                         >  SRR19973070.794092/1‑150 (MQ=60)
                                              GACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGC                                                                                                        <  SRR19973070.875147/150‑1 (MQ=60)
                                                CGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCC                                                                                                      <  SRR19973070.457464/150‑1 (MQ=60)
                                                      TCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCG                                                                                                <  SRR19973070.2863062/150‑1 (MQ=60)
                                                      TCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCG                                                                                                <  SRR19973070.2864580/150‑1 (MQ=60)
                                                      TCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCG                                                                                                <  SRR19973070.734710/150‑1 (MQ=60)
                                                          GTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAAC                                                                                            >  SRR19973070.2472408/1‑150 (MQ=60)
                                                                 GGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCC                                                                                     <  SRR19973070.373617/150‑1 (MQ=60)
                                                                    AATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAA                                                                                  >  SRR19973070.1277922/1‑150 (MQ=60)
                                                                      TACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAG                                                                                <  SRR19973070.1929955/150‑1 (MQ=60)
                                                                      TACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAG                                                                                <  SRR19973070.1338749/150‑1 (MQ=60)
                                                                        CTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAG                                                                              <  SRR19973070.122011/150‑1 (MQ=60)
                                                                          CCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAG                                                                            <  SRR19973070.2480579/150‑1 (MQ=60)
                                                                          CCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAG                                                                            <  SRR19973070.2480575/150‑1 (MQ=60)
                                                                             CATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTT                                                                         >  SRR19973070.1982998/1‑150 (MQ=60)
                                                                             CATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTT                                                                         >  SRR19973070.2927639/1‑150 (MQ=60)
                                                                                CACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAA                                                                      >  SRR19973070.956542/1‑150 (MQ=60)
                                                                                   CACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCC                                                                   >  SRR19973070.2826579/1‑150 (MQ=60)
                                                                                    ACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCG                                                                  <  SRR19973070.2895402/150‑1 (MQ=60)
                                                                                      GCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAC                                                                >  SRR19973070.1937337/1‑150 (MQ=60)
                                                                                       CTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACA                                                               <  SRR19973070.2135797/150‑1 (MQ=60)
                                                                                        TTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAG                                                              <  SRR19973070.725361/150‑1 (MQ=60)
                                                                                         TGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGC                                                             >  SRR19973070.271805/1‑150 (MQ=60)
                                                                                         TGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGC                                                             >  SRR19973070.272203/1‑150 (MQ=60)
                                                                                         TGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGC                                                             >  SRR19973070.271150/1‑150 (MQ=60)
                                                                                           GGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAG                                                           >  SRR19973070.2264595/1‑150 (MQ=60)
                                                                                           GGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAG                                                           <  SRR19973070.2576106/150‑1 (MQ=60)
                                                                                               GTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGT                                                       <  SRR19973070.3030453/150‑1 (MQ=60)
                                                                                               GTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGT                                                       <  SRR19973070.2061239/150‑1 (MQ=60)
                                                                                               GTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGT                                                       <  SRR19973070.3118643/150‑1 (MQ=60)
                                                                                               GTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGT                                                       <  SRR19973070.893997/150‑1 (MQ=60)
                                                                                                TTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTT                                                      <  SRR19973070.29946/150‑1 (MQ=60)
                                                                                                    TGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCAT                                                  >  SRR19973070.2172600/1‑150 (MQ=60)
                                                                                                    TGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCAT                                                  <  SRR19973070.2291775/150‑1 (MQ=60)
                                                                                                    TGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTCCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCAT                                                  <  SRR19973070.621369/150‑1 (MQ=60)
                                                                                                        ACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAAT                                              <  SRR19973070.896661/150‑1 (MQ=60)
                                                                                                        ACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAAT                                              <  SRR19973070.3022065/150‑1 (MQ=60)
                                                                                                          GCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCA                                            <  SRR19973070.2539895/150‑1 (MQ=60)
                                                                                                           CGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCAC                                           >  SRR19973070.1128674/1‑150 (MQ=60)
                                                                                                             ctttcatctaTTTAATCGCCTGTAAGTGCGCTTGCTGTGTGTCCGCGTTGAATGCATCTTGGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAATAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCA                                         <  SRR19973070.1901170/140‑1 (MQ=60)
                                                                                                              TATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCAC                                        >  SRR19973070.852739/1‑150 (MQ=60)
                                                                                                                 CAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGAT                                     <  SRR19973070.2665064/150‑1 (MQ=60)
                                                                                                                 CAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGAT                                     <  SRR19973070.2665049/150‑1 (MQ=60)
                                                                                                                  AGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATG                                    <  SRR19973070.523316/150‑1 (MQ=60)
                                                                                                                      CTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCAT                                <  SRR19973070.278065/150‑1 (MQ=60)
                                                                                                                      CTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCAT                                <  SRR19973070.277941/150‑1 (MQ=60)
                                                                                                                        TTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGT                              >  SRR19973070.1948958/1‑150 (MQ=60)
                                                                                                                        TTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGT                              <  SRR19973070.2604107/150‑1 (MQ=60)
                                                                                                                           ATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCA                           <  SRR19973070.2656002/150‑1 (MQ=60)
                                                                                                                             CGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATC                         >  SRR19973070.1645098/1‑150 (MQ=60)
                                                                                                                             CGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATC                         >  SRR19973070.3292794/1‑150 (MQ=60)
                                                                                                                              GCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCT                        <  SRR19973070.405479/150‑1 (MQ=60)
                                                                                                                               CCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTG                       >  SRR19973070.2133873/1‑150 (MQ=60)
                                                                                                                                CTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGC                      >  SRR19973070.648936/1‑150 (MQ=60)
                                                                                                                                 TGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCC                     >  SRR19973070.3100280/1‑150 (MQ=60)
                                                                                                                                   TAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCA                   <  SRR19973070.1584637/150‑1 (MQ=60)
                                                                                                                                     AGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGT                 >  SRR19973070.913204/1‑150 (MQ=60)
                                                                                                                                            TTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAT          <  SRR19973070.2986786/150‑1 (MQ=60)
                                                                                                                                            TTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAT          <  SRR19973070.1496516/150‑1 (MQ=60)
                                                                                                                                              GCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCT        >  SRR19973070.1779068/1‑150 (MQ=60)
                                                                                                                                                TGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCTCGATGCCATGTTCATCTGCCCAGTCGAGCATCTCT      <  SRR19973070.949665/150‑1 (MQ=60)
                                                                                                                                                  AGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTC    <  SRR19973070.2580831/150‑1 (MQ=60)
                                                                                                                                                  AGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTC    >  SRR19973070.2272419/1‑150 (MQ=60)
                                                                                                                                                    TGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCAG  <  SRR19973070.2856098/150‑1 (MQ=60)
                                                                                                                                                    TGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCAG  <  SRR19973070.372325/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTTCAG  >  CP009273/1689031‑1689328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑