Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,825,763 C→A 100% A159A (GCG→GCT)  astC ← succinylornithine transaminase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,825,7630CA100.0% 502.7 / NA 150A159A (GCG→GCT) astCsuccinylornithine transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (88/62);  total (88/62)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 150 total aligned reads displayed.

TGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCC  >  CP009273/1825620‑1825907
                                                                                                                                               |                                                                                                                                                
   aaaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAt                                                                                                                                           >  1:257146/1‑148 (MQ=255)
   aaaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAt                                                                                                                                           <  2:628799/148‑1 (MQ=255)
   aaaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAt                                                                                                                                           >  2:1807100/1‑148 (MQ=255)
   aaaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAt                                                                                                                                           <  2:163219/148‑1 (MQ=255)
    aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGTAGCaaaaac                                                                                                                                          >  1:2420608/1‑146 (MQ=255)
    aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATc                                                                                                                                          >  2:1750562/1‑148 (MQ=255)
    aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATc                                                                                                                                          >  2:1750609/1‑148 (MQ=255)
       aCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCtg                                                                                                                                       >  2:3147000/1‑148 (MQ=255)
       aCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCtg                                                                                                                                       >  1:3245893/1‑148 (MQ=255)
        cgcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTCGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCtgt                                                                                                                                      >  1:2832836/1‑148 (MQ=255)
               ctggctggCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAgg                                                                                                                               >  2:361782/1‑148 (MQ=255)
               ctggctggCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAgg                                                                                                                               <  1:730148/148‑1 (MQ=255)
                tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggc                                                                                                                              <  2:1800306/148‑1 (MQ=255)
                tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggc                                                                                                                              <  2:2912377/148‑1 (MQ=255)
                tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggc                                                                                                                              <  2:1306143/148‑1 (MQ=255)
                  gctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTCGAGTCGTCAATCAGCGCGCTGGCAGAGTTAAAATCGTTATATGCAGCATGAAGAATATCCCCCGGCAGTGGAGCAAAATCATGTCAAAAggctg                                                                                                                            >  1:2640685/1‑148 (MQ=255)
                  gctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctg                                                                                                                            >  1:2640928/1‑148 (MQ=255)
                   ctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctgg                                                                                                                           <  2:900871/148‑1 (MQ=255)
                    tggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctggc                                                                                                                          <  1:2323699/148‑1 (MQ=255)
                    tggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctggc                                                                                                                          <  1:310693/148‑1 (MQ=255)
                        accacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGcc                                                                                                                      >  2:1097928/1‑148 (MQ=255)
                        accacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGcc                                                                                                                      >  2:8003/1‑148 (MQ=255)
                        accacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGcc                                                                                                                      >  2:2286770/1‑148 (MQ=255)
                         ccacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGccc                                                                                                                     >  1:2966158/1‑148 (MQ=255)
                              ccGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCg                                                                                                                >  2:2985915/1‑148 (MQ=255)
                                gCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCATGTGAATAGGCTGGCTGCCCACCCGCa                                                                                                              <  1:1932655/148‑1 (MQ=255)
                                  cTTCCCCCTGGCTGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATAGCGTTATATGCAGCATGACGACTATCCGCCGGCAGTGGAGCAAAATCCGGTGACTAGGCTGGCTGCCCACCCGCCCt                                                                                                            >  2:1999127/1‑148 (MQ=255)
                                     cccccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGAc                                                                                                         <  2:1731591/148‑1 (MQ=255)
                                        ccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGt                                                                                                      >  2:1944882/1‑148 (MQ=255)
                                            gATGGGTTCGACAATCACCGCTCAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAc                                                                                                  >  2:1582024/1‑148 (MQ=255)
                                            gATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAc                                                                                                  >  2:2644470/1‑148 (MQ=255)
                                            gATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAc                                                                                                  >  2:1581448/1‑148 (MQ=255)
                                                   tCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGc                                                                                           >  1:1515238/1‑148 (MQ=255)
                                                     gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGa                                                                                         <  1:2194535/148‑1 (MQ=255)
                                                     gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGa                                                                                         <  1:8003/148‑1 (MQ=255)
                                                     gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGa                                                                                         <  1:2286770/148‑1 (MQ=255)
                                                      aCAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAc                                                                                        <  2:2678356/148‑1 (MQ=255)
                                                          tCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATg                                                                                    <  1:231413/148‑1 (MQ=255)
                                                           cACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGa                                                                                   <  2:2901636/148‑1 (MQ=255)
                                                                 acaGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGTGTGCGACCATGAAACGCa                                                                             <  1:3240358/148‑1 (MQ=255)
                                                                    ggTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttt                                                                          <  1:987391/148‑1 (MQ=255)
                                                                     gTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtttt                                                                         >  2:2086221/1‑148 (MQ=255)
                                                                      tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttttt                                                                        >  1:1600635/1‑148 (MQ=255)
                                                                        gagTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                      <  1:1549520/148‑1 (MQ=255)
                                                                        gagTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                      <  1:1828019/148‑1 (MQ=255)
                                                                             gtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGcc                                                                 >  2:3297668/1‑148 (MQ=255)
                                                                             gtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGcc                                                                 <  1:1177553/148‑1 (MQ=255)
                                                                              tcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCa                                                                >  2:2400523/1‑148 (MQ=255)
                                                                               cAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAc                                                               >  1:1859509/1‑148 (MQ=255)
                                                                               cAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAc                                                               >  1:189082/1‑148 (MQ=255)
                                                                                         gcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctc                                                     <  2:1017875/148‑1 (MQ=255)
                                                                                          cTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctct                                                    <  1:580044/148‑1 (MQ=255)
                                                                                              cAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATg                                                >  1:1693405/1‑148 (MQ=255)
                                                                                              cAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATg                                                >  1:17210/1‑148 (MQ=255)
                                                                                               agagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATgg                                               <  2:2741790/148‑1 (MQ=255)
                                                                                                gagTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGc                                              >  1:2152972/1‑148 (MQ=255)
                                                                                                 agTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTGATGGCt                                             >  2:2830403/1‑148 (MQ=255)
                                                                                                   ttAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGc                                           >  1:353925/1‑148 (MQ=255)
                                                                                                   ttAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGc                                           >  2:1165798/1‑148 (MQ=255)
                                                                                                   ttAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGc                                           >  2:432055/1‑148 (MQ=255)
                                                                                                      atatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGt                                        >  2:1447494/1‑148 (MQ=255)
                                                                                                       taccGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                       >  2:2682755/4‑148 (MQ=255)
                                                                                                        atCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAg                                      >  2:2800576/1‑148 (MQ=255)
                                                                                                         tCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGc                                     >  2:2754342/1‑148 (MQ=255)
                                                                                                         tCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGc                                     >  2:2880919/1‑148 (MQ=255)
                                                                                                           gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCgg                                   <  2:2388993/148‑1 (MQ=255)
                                                                                                            ttATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGt                                  <  2:1242104/148‑1 (MQ=255)
                                                                                                                atGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                              >  2:2993579/1‑148 (MQ=255)
                                                                                                                atGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                              >  2:56914/1‑148 (MQ=255)
                                                                                                                atGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                              >  2:56763/1‑148 (MQ=255)
                                                                                                                atGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                              >  1:2527722/1‑148 (MQ=255)
                                                                                                                     gcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGa                         <  2:1859509/148‑1 (MQ=255)
                                                                                                                     gcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGa                         <  2:189082/148‑1 (MQ=255)
                                                                                                                      caTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGaa                        >  1:1319608/1‑148 (MQ=255)
                                                                                                                       aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAt                       <  2:3025829/148‑1 (MQ=255)
                                                                                                                        tGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                      >  1:2710775/1‑148 (MQ=255)
                                                                                                                         gACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAttt                     >  2:614785/1‑148 (MQ=255)
                                                                                                                              atatCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcgcg                >  1:572345/1‑148 (MQ=255)
                                                                                                                              atatCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcgcg                >  1:343427/1‑148 (MQ=255)
                                                                                                                               tatCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACgcgc               >  2:1441548/1‑148 (MQ=255)
                                                                                                                                atCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGcc              <  2:3239086/148‑1 (MQ=255)
                                                                                                                                agccgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGcc              >  1:3109692/3‑148 (MQ=255)
                                                                                                                                 tCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCa             >  2:2277025/1‑148 (MQ=255)
                                                                                                                                  ccgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAg            <  1:448267/148‑1 (MQ=255)
                                                                                                                                  ccgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAg            >  2:549604/1‑148 (MQ=255)
                                                                                                                                  ccgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAg            >  1:265263/1‑148 (MQ=255)
                                                                                                                                  ccgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAg            <  1:448284/148‑1 (MQ=255)
                                                                                                                                  ccgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATGTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGGCGTGAGCGAATTTACGCGCCAg            >  2:2734275/1‑148 (MQ=255)
                                                                                                                                   cgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGt           <  1:1501495/148‑1 (MQ=255)
                                                                                                                                     ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGttt         <  2:3245893/148‑1 (MQ=255)
                                                                                                                                      cgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGtttt        <  1:1000489/148‑1 (MQ=255)
                                                                                                                                      cgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGtttt        <  2:257146/148‑1 (MQ=255)
                                                                                                                                      cgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGGAGCGGTCGTGAGCGAATTTACGCGCCAGtttt        <  1:124168/148‑1 (MQ=255)
                                                                                                                                       ggCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTa       <  1:2553779/148‑1 (MQ=255)
                                                                                                                                        gCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAg      <  2:3115616/148‑1 (MQ=255)
                                                                                                                                        gCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAg      >  2:2488333/1‑148 (MQ=255)
                                                                                                                                        gCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAg      >  2:2231878/1‑148 (MQ=255)
                                                                                                                                           gTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAgcgc   <  1:2254247/148‑1 (MQ=255)
                                                                                                                                            tGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGcgcc  >  1:3168706/1‑148 (MQ=255)
                                                                                                                                            tGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGcgcc  >  1:3168719/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCC  >  CP009273/1825620‑1825907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCT  >  CP009273/1825618‑1825910
                                                                                                                                                 |                                                                                                                                                   
CTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAA                                                                                                                                                 >  SRR19973070.2404770/1‑150 (MQ=60)
   GTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAT                                                                                                                                              >  SRR19973070.258877/1‑150 (MQ=60)
     AAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCC                                                                                                                                            <  SRR19973070.2245931/150‑1 (MQ=60)
      AAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCT                                                                                                                                           <  SRR19973070.1358657/150‑1 (MQ=60)
       AAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTG                                                                                                                                          >  SRR19973070.3266811/1‑150 (MQ=60)
        AACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTCGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGT                                                                                                                                         >  SRR19973070.2851170/1‑150 (MQ=60)
        AACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGTGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGT                                                                                                                                         >  SRR19973070.1223886/1‑150 (MQ=60)
                GCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGC                                                                                                                                 <  SRR19973070.3173631/150‑1 (MQ=60)
                 CTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCT                                                                                                                                <  SRR19973070.735325/150‑1 (MQ=60)
                  TGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTCGAGTCGTCAATCAGCGCGCTGGCAGAGTTAAAATCGTTATATGCAGCATGAAGAATATCCCCCGGCAGTGGAGCAAAATCATGTCAAAAGGCTG                                                                                                                               >  SRR19973070.2657892/1‑150 (MQ=60)
                  TGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTG                                                                                                                               >  SRR19973070.2657748/1‑150 (MQ=60)
                  TGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTG                                                                                                                               >  SRR19973070.2658136/1‑150 (MQ=60)
                   GGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGG                                                                                                                              <  SRR19973070.3274074/150‑1 (MQ=60)
                      TGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTG                                                                                                                           <  SRR19973070.2339142/150‑1 (MQ=60)
                      TGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTG                                                                                                                           <  SRR19973070.312919/150‑1 (MQ=60)
                       GGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCACTCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGC                                                                                                                          >  SRR19973070.3259981/1‑150 (MQ=60)
                         CACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCC                                                                                                                        >  SRR19973070.2985223/1‑150 (MQ=60)
                               ACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGC                                                                                                                  >  SRR19973070.980469/1‑150 (MQ=60)
                                  GCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCATGTGAATAGGCTGGCTGCCCACCCGCACT                                                                                                               <  SRR19973070.1945663/150‑1 (MQ=60)
                                        CCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGT                                                                                                         >  SRR19973070.1547932/1‑150 (MQ=60)
                                                   GTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGC                                                                                              >  SRR19973070.1525853/1‑150 (MQ=60)
                                                       GACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACC                                                                                          <  SRR19973070.8118/150‑1 (MQ=60)
                                                       GACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACC                                                                                          <  SRR19973070.2209024/150‑1 (MQ=60)
                                                       GACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACC                                                                                          <  SRR19973070.2301980/150‑1 (MQ=60)
                                                        ACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCA                                                                                         <  SRR19973070.1819572/150‑1 (MQ=60)
                                                            TCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAA                                                                                     <  SRR19973070.232910/150‑1 (MQ=60)
                                                            TCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAA                                                                                     <  SRR19973070.233046/150‑1 (MQ=60)
                                                                   ACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGTGTGCGACCATGAAACGCATT                                                                              <  SRR19973070.3261258/150‑1 (MQ=60)
                                                                      GGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTT                                                                           <  SRR19973070.994426/150‑1 (MQ=60)
                                                                      GGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTT                                                                           >  SRR19973070.1611813/1‑150 (MQ=60)
                                                                      GGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCAGTGAATATGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTT                                                                           >  SRR19973070.662443/1‑150 (MQ=60)
                                                                       GTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTG                                                                          <  SRR19973070.2780754/150‑1 (MQ=60)
                                                                          GAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAAC                                                                       <  SRR19973070.1840589/150‑1 (MQ=60)
                                                                          GAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAAC                                                                       <  SRR19973070.1560419/150‑1 (MQ=60)
                                                                               GTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAC                                                                  <  SRR19973070.1185812/150‑1 (MQ=60)
                                                                               GTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAC                                                                  >  SRR19973070.1872232/1‑150 (MQ=60)
                                                                               GTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAC                                                                  >  SRR19973070.190545/1‑150 (MQ=60)
                                                                                        CGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCT                                                         >  SRR19973070.1877141/1‑150 (MQ=60)
                                                                                            CTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTA                                                     <  SRR19973070.584037/150‑1 (MQ=60)
                                                                                             TGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAT                                                    >  SRR19973070.1953031/1‑150 (MQ=60)
                                                                                              GGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATG                                                   >  SRR19973070.1705050/1‑150 (MQ=60)
                                                                                              GGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATG                                                   >  SRR19973070.17463/1‑150 (MQ=60)
                                                                                                CAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGC                                                 >  SRR19973070.2167300/1‑150 (MQ=60)
                                                                                                   AGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGC                                              >  SRR19973070.356502/1‑150 (MQ=60)
                                                                                                                ATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTG                                 >  SRR19973070.2544543/1‑150 (MQ=60)
                                                                                                                ATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTG                                 >  SRR19973070.2544228/1‑150 (MQ=60)
                                                                                                                  ATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAG                               <  SRR19973070.364430/150‑1 (MQ=60)
                                                                                                                      AGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAA                           >  SRR19973070.1328736/1‑150 (MQ=60)
                                                                                                                        CATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATT                         >  SRR19973070.2728298/1‑150 (MQ=60)
                                                                                                                        CATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATT                         <  SRR19973070.1650817/150‑1 (MQ=60)
                                                                                                                          TGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTA                       <  SRR19973070.3011910/150‑1 (MQ=60)
                                                                                                                              GAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCG                   >  SRR19973070.576244/1‑150 (MQ=60)
                                                                                                                              GAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCG                   >  SRR19973070.345931/1‑150 (MQ=60)
                                                                                                                              GAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCG                   >  SRR19973070.576226/1‑150 (MQ=60)
                                                                                                                                ATAGCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCC                 >  SRR19973070.3129751/1‑150 (MQ=60)
                                                                                                                                  ATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAG               >  SRR19973070.267023/1‑150 (MQ=60)
                                                                                                                                  ATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAG               >  SRR19973070.266786/1‑150 (MQ=60)
                                                                                                                                    CCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTT             <  SRR19973070.451502/150‑1 (MQ=60)
                                                                                                                                    CCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTT             <  SRR19973070.451519/150‑1 (MQ=60)
                                                                                                                                     CGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTT            <  SRR19973070.1512009/150‑1 (MQ=60)
                                                                                                                                        CGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAG         <  SRR19973070.1007619/150‑1 (MQ=60)
                                                                                                                                        CGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGGAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAG         <  SRR19973070.125269/150‑1 (MQ=60)
                                                                                                                                         GGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGC        <  SRR19973070.2570474/150‑1 (MQ=60)
                                                                                                                                            AGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCC     >  SRR19973070.3189222/1‑150 (MQ=60)
                                                                                                                                            AGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCC     >  SRR19973070.3189209/1‑150 (MQ=60)
                                                                                                                                             GTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCG    <  SRR19973070.2269197/150‑1 (MQ=60)
                                                                                                                                               GGAGCAAAATCCTGTTAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGAATTTTTGAACGCCACGATGCCGCTCTTTTGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCT  <  SRR19973070.1762741/150‑1 (MQ=60)
                                                                                                                                               GGAGCAAAATCCTGTGAATTGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCT  <  SRR19973070.1762694/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
CTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGCT  >  CP009273/1825618‑1825910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑