Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,669,592 G→T 100% F342L (TTC→TTA)  pntB ← pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,669,5920GT96.0% 518.8 / 12.0 175F342L (TTC→TTA) pntBpyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (6/1);  new base T (85/82);  total (91/84)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 175 total aligned reads displayed.

TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTG  >  CP009273/1669447‑1669736
                                                                                                                                                 |                                                                                                                                                
tAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaa                                                                                                                                                >  1:567438/1‑148 (MQ=255)
 aGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaaa                                                                                                                                               <  2:1084449/148‑1 (MQ=255)
  gCACCAATCCCCAGTCCGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCACCACGATGTCATACGGTACTTTTGCTACAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAAc                                                                                                                                              <  1:1877306/148‑1 (MQ=255)
  gCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAAc                                                                                                                                              >  2:818676/1‑148 (MQ=255)
     ccAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATAACTAAACGca                                                                                                                                           >  1:1985109/1‑148 (MQ=255)
       aaTCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGcaca                                                                                                                                         >  2:1315191/1‑148 (MQ=255)
          cACCAGTACGTTATCGGTATCAGCAACTTCATCTTTTATCTCGTCCATTTCCAGCACTTTTTCATAGGGTATTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTa                                                                                                                                      <  1:1022542/148‑1 (MQ=255)
          cACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTa                                                                                                                                      >  1:1332508/1‑148 (MQ=255)
          cACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTa                                                                                                                                      <  2:1787823/148‑1 (MQ=255)
            ccAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAt                                                                                                                                    >  2:2389979/1‑148 (MQ=255)
            ccAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAt                                                                                                                                    <  2:1443997/148‑1 (MQ=255)
            ccAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAt                                                                                                                                    >  2:1230287/1‑148 (MQ=255)
                tACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGTTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCa                                                                                                                                <  1:182733/148‑1 (MQ=255)
                     tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgc                                                                                                                           >  2:2442113/1‑148 (MQ=255)
                      atcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCCGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcg                                                                                                                          <  2:1658482/148‑1 (MQ=255)
                          gtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCa                                                                                                                      >  2:677192/1‑148 (MQ=255)
                            atcAGCAAAGTCATCATTTATCTCTTCATTTTCCACCACGATGTCATAGGGTACTTTTGCTACAGCCAGCAATANGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAt                                                                                                                    <  1:1806709/148‑1 (MQ=255)
                            atcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAt                                                                                                                    >  2:3483040/1‑148 (MQ=255)
                             tcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAtt                                                                                                                   >  1:2959969/1‑148 (MQ=255)
                              cAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTAGCTTCAGACAGCAATACGATCATATGTCCAGTCAAACGACCCGAGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAttt                                                                                                                  >  1:2221275/1‑148 (MQ=255)
                                gCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtct                                                                                                                <  2:1342032/148‑1 (MQ=255)
                                gCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtct                                                                                                                <  2:1342044/148‑1 (MQ=255)
                                 aaaaTTCACCATTTATCTCGTCCATTTCCAGCACTATGTCATCCGGTAATTTTGCTTCAGCCACAAATACGTTCAAAGGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtctc                                                                                                               <  1:615139/147‑1 (MQ=255)
                                   aaGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAg                                                                                                             >  2:917628/1‑148 (MQ=255)
                                    aGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGt                                                                                                            >  1:711813/1‑148 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                          >  2:1046501/1‑148 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                          >  2:3523440/1‑148 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                          <  2:2447655/148‑1 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                          <  2:3103074/148‑1 (MQ=255)
                                       catcatTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAAt                                                                                                         <  1:594295/148‑1 (MQ=255)
                                          catTGATCTCGGCCATTTCCCGCACGATTTCATAGGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTc                                                                                                      <  1:1826340/148‑1 (MQ=255)
                                            ttGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                    <  2:2558047/148‑1 (MQ=255)
                                               aTCTCGTCAATTTCCAGCACGATGTCATAGGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                                 <  1:3091636/148‑1 (MQ=255)
                                                tctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAGTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                                >  2:2624366/1‑148 (MQ=255)
                                                 ctggTCCTTTTCCAGCACTTTGTCATACGTTATTTTTGCTTCAGCCAGCAATAGGTTATTTTGTCCCGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCAATTTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                               <  1:180457/145‑1 (MQ=255)
                                                 ctcGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                               >  1:908736/1‑148 (MQ=255)
                                                 ctcGTCATTTTCCAGCACTATGTCATACGGTACTTTTGCTTCAGCCAGAAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACa                                                                                               <  1:3551105/148‑1 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                             <  2:2194899/148‑1 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                             >  2:1185156/1‑148 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                             <  2:2577299/148‑1 (MQ=255)
                                                      ccATTTCCAGCACTATGTCATACGTTACTTTTGCTTCAGCCAGAAATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGata                                                                                          <  1:3526140/148‑1 (MQ=255)
                                                        aTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAtt                                                                                        >  1:2309828/1‑148 (MQ=255)
                                                         tttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTg                                                                                       >  2:203088/1‑148 (MQ=255)
                                                         tttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTg                                                                                       >  2:204062/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                      >  2:1015378/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                      >  2:1017580/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                      >  1:2868028/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                      >  2:1707136/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                      >  1:2530469/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                              >  1:1348630/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                              >  2:2377272/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                              >  2:652003/1‑148 (MQ=255)
                                                                           aCGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCAt                                                                     >  2:651205/1‑148 (MQ=255)
                                                                           aCGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCAt                                                                     >  2:651280/1‑148 (MQ=255)
                                                                             gttACTTTTGCTTCAGCCAGCAAGACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTACACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGc                                                                   <  2:1780101/146‑1 (MQ=255)
                                                                               tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCg                                                                 >  2:581574/1‑148 (MQ=255)
                                                                                aCTTTTGCTTCAGCCAGCAATCCGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                                <  2:2530528/148‑1 (MQ=255)
                                                                                 cTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTa                                                               >  2:402663/1‑148 (MQ=255)
                                                                                    ttGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTAccc                                                            <  2:1244558/148‑1 (MQ=255)
                                                                                       cTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCgg                                                         >  1:3287646/1‑148 (MQ=255)
                                                                                        ttCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGa                                                        >  2:1775180/1‑148 (MQ=255)
                                                                                         tccgccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                       <  1:2083612/145‑1 (MQ=255)
                                                                                         tCAGCCAGCAATCCGTTCATATGTCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTGCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                       <  2:1546213/148‑1 (MQ=255)
                                                                                         tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                       >  2:1397730/1‑148 (MQ=255)
                                                                                           agccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTa                                                     <  1:1465554/148‑1 (MQ=255)
                                                                                            gccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGCGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTaa                                                    <  1:3482516/148‑1 (MQ=255)
                                                                                             ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                   >  1:204228/1‑148 (MQ=255)
                                                                                             ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                   >  2:1890183/1‑148 (MQ=255)
                                                                                              cagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATg                                                  >  1:1502994/1‑148 (MQ=255)
                                                                                               agcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGa                                                 <  1:520334/148‑1 (MQ=255)
                                                                                                 cAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATc                                               >  1:3224359/1‑148 (MQ=255)
                                                                                                        ttCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAAt                                        <  2:1763168/148‑1 (MQ=255)
                                                                                                        ttCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAAt                                        <  2:452119/148‑1 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                       <  2:649905/148‑1 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                       >  2:2168488/1‑148 (MQ=255)
                                                                                                          cATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATgg                                      <  1:2347512/148‑1 (MQ=255)
                                                                                                           atatGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                     >  1:1932097/1‑148 (MQ=255)
                                                                                                             atGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAg                                   >  2:2767468/1‑148 (MQ=255)
                                                                                                                 ccAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                               <  2:1480986/148‑1 (MQ=255)
                                                                                                                  cAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttttt                              <  1:1164555/148‑1 (MQ=255)
                                                                                                                      cAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagc                          <  2:2959969/148‑1 (MQ=255)
                                                                                                                        aaCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                        <  1:2682816/148‑1 (MQ=255)
                                                                                                                         aCGCCCCGCGACCGGTTGGATAAATAAACGCACATTAATACCACGATCGCGCAATTTCTAAGTATTTTAAGCGACAGTATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                       <  1:1560274/148‑1 (MQ=255)
                                                                                                                         aCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGt                       >  2:212172/1‑148 (MQ=255)
                                                                                                                          ccccccGCGACGGGGTGGATACATAAACGCACATTAATACCCCGAGCGCGCAATTTCACAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGtt                      <  1:1230339/146‑1 (MQ=255)
                                                                                                                           gCCCCGCGACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTc                     <  2:2017952/148‑1 (MQ=255)
                                                                                                                             cccGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCg                   <  2:1395041/148‑1 (MQ=255)
                                                                                                                              ccGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGc                  >  2:2177769/1‑148 (MQ=255)
                                                                                                                               cgcgACCGGGTGGATACCTAAACGCACATTAATACAACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCt                 >  1:2282640/1‑148 (MQ=255)
                                                                                                                                 cgACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGt               <  1:2406082/148‑1 (MQ=255)
                                                                                                                                 cgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGt               >  1:3063363/1‑148 (MQ=255)
                                                                                                                                    ccGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtctc            <  2:1407621/148‑1 (MQ=255)
                                                                                                                                      gggTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtctt          <  1:435276/148‑1 (MQ=255)
                                                                                                                                      gggTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtctt          <  1:435361/148‑1 (MQ=255)
                                                                                                                                        gTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtcttct        >  2:565989/1‑148 (MQ=255)
                                                                                                                                         tGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTg       <  2:2751640/148‑1 (MQ=255)
                                                                                                                                         tGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTg       <  1:3452118/148‑1 (MQ=255)
                                                                                                                                           gATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCATCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCg     >  1:1089239/1‑148 (MQ=255)
                                                                                                                                           gATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCg     <  1:1496319/148‑1 (MQ=255)
                                                                                                                                            aTACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCgg    <  2:2833309/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTG  >  CP009273/1669447‑1669736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  >  CP009273/1669445‑1669740
                                                                                                                                                   |                                                                                                                                                    
GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAA                                                                                                                                                    >  SRR19973067.571131/1‑150 (MQ=60)
    GCACCAATCCCCAGTCCGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCACCACGATGTCATACGGTACTTTTGCTACAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGC                                                                                                                                                <  SRR19973067.1890113/150‑1 (MQ=60)
     CACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATAACTAAACGCA                                                                                                                                               >  SRR19973067.1998543/1‑150 (MQ=60)
          ATCCCCAGTACGTTATCGTTATCAGAAATGTAATCATTGATCTGGTCATTTTCCAGCACTTTGTAATACGGTACTTTTGATTCAGCAAGAAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTA                                                                                                                                          <  SRR19973067.59152/150‑1 (MQ=60)
          ATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTA                                                                                                                                          >  SRR19973067.1341604/1‑150 (MQ=60)
            CACCAGTACGTTATCGGTATCAGCAACTTCATCTTTTATCTCGTCCATTTCCAGCACTTTTTCATAGGGTATTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAT                                                                                                                                        <  SRR19973067.1029569/150‑1 (MQ=60)
            CACCAGTACGGTATCGTTATCAGAAAAGTAATAATTGATCTCGTCCTTTTCCAGCACGATTTAATCCGGTATTTTTGCTTCACCCAGCAATACGTTCATAGGTCCCGCCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAAT                                                                                                                                        <  SRR19973067.1029932/150‑1 (MQ=60)
                  TACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGTTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACG                                                                                                                                  <  SRR19973067.184182/150‑1 (MQ=60)
                      GTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCG                                                                                                                              >  SRR19973067.3465897/1‑150 (MQ=60)
                             TATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATT                                                                                                                       >  SRR19973067.2978668/1‑150 (MQ=60)
                              ATCAGCAAAGTCATCATTTATCTCTTCATTTTCCACCACGATGTCATAGGGTACTTTTGCTACAGCCAGCAATANGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTT                                                                                                                      <  SRR19973067.1818902/150‑1 (MQ=60)
                              ATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTAGCTTCAGACAGCAATACGATCATATGTCCAGTCAAACGACCCGAGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTT                                                                                                                      >  SRR19973067.2236059/1‑150 (MQ=60)
                                   aaaattcaccatttATCTCGTCCATTTCCAGCACTATGTCATCCGGTAATTTTGCTTCAGCCACAAATACGTTCAAAGGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAG                                                                                                                 <  SRR19973067.619150/136‑1 (MQ=60)
                                    AAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGT                                                                                                                >  SRR19973067.716742/1‑150 (MQ=60)
                                    AAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGT                                                                                                                >  SRR19973067.716577/1‑150 (MQ=60)
                                         CATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTT                                                                                                           <  SRR19973067.598071/150‑1 (MQ=60)
                                            CATTGATCTCGGCCATTTCCCGCACGATTTCATAGGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                        <  SRR19973067.1838747/150‑1 (MQ=60)
                                                 ATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACA                                                                                                   >  SRR19973067.914762/1‑150 (MQ=60)
                                                 ATCTCGTCAATTTCCAGCACGATGTCATAGGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACA                                                                                                   <  SRR19973067.3111303/150‑1 (MQ=60)
                                                  TCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAG                                                                                                  <  SRR19973067.3573171/150‑1 (MQ=60)
                                                   CTGGTCCTTTTCCAGCACTTTGTCATACGTTATTTTTGCTTCAGCCAGCAATAGGTTATTTTGTCCCGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCAATTTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGG                                                                                                 <  SRR19973067.181891/150‑1 (MQ=60)
                                                   CTCGTCCATTTCCAGCACTTTGTCATAGGGTTTTTTTGTTTCAGCCAGAAATCCGTTCTTTTGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCATTTTCTCAGTAATTTCAGCGACAGG                                                                                                 <  SRR19973067.182084/150‑1 (MQ=60)
                                                   CTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATAGGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGG                                                                                                 <  SRR19973067.1805073/150‑1 (MQ=60)
                                                   CTCGTCATTTTCCAGCACTATGTCATACGGTACTTTTGCTTCAGCCAGAAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGG                                                                                                 <  SRR19973067.3573501/150‑1 (MQ=60)
                                                     ggtcaatttccagcactttgtcttaggttatTTTTGCTTCAGCCAGAAATAGGTTCATATGTCCAGGAAACCGCCCCGCGACCGGGTGGATACCTAAACGCAAATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGNT                                                                                               <  SRR19973067.833846/119‑1 (MQ=60)
                                                     CGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGAT                                                                                               >  SRR19973067.1334727/1‑150 (MQ=60)
                                                     CGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATATCAGCGACAGGAT                                                                                               >  SRR19973067.208042/1‑150 (MQ=60)
                                                        CCATTTCCAGCACTATGTCATACGTTACTTTTGCTTCAGCCAGAAATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                            <  SRR19973067.3548444/150‑1 (MQ=60)
                                                        CCATTTCCAGCACGATGTCATAGGGTAATTTTGATTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                            <  SRR19973067.3548337/150‑1 (MQ=60)
                                                        CCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                            >  SRR19973067.2325042/1‑150 (MQ=60)
                                                        CCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATT                                                                                            >  SRR19973067.2325226/1‑150 (MQ=60)
                                                          ttttccaccacttttaaatagggtatttttgcttccgccagaaatagttaaatatggcccggaaacccCCCCGCGACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                          <  SRR19973067.2445848/82‑1 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                          >  SRR19973067.3125552/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                          >  SRR19973067.2886393/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                          >  SRR19973067.1228912/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                          >  SRR19973067.2547049/1‑150 (MQ=60)
                                                                  CACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGC                                                                                  >  SRR19973067.1357851/1‑150 (MQ=60)
                                                                  CACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGC                                                                                  >  SRR19973067.2410367/1‑150 (MQ=60)
                                                                         TCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCC                                                                           >  SRR19973067.226760/1‑150 (MQ=60)
                                                                                   ATTTTGCTTCCGCCAGAAATCCTTTCATATGACCAGCAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACC                                                                 <  SRR19973067.1370708/150‑1 (MQ=60)
                                                                                    ttttgctcccgccccaaagccgttcttcggtccaggcaccccCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCC                                                                <  SRR19973067.1256169/108‑1 (MQ=60)
                                                                                       TGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGG                                                             >  SRR19973067.3308298/1‑150 (MQ=60)
                                                                                       TGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGG                                                             >  SRR19973067.3308258/1‑150 (MQ=60)
                                                                                           TCCGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTA                                                         <  SRR19973067.2097413/150‑1 (MQ=60)
                                                                                           TCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTA                                                         >  SRR19973067.182135/1‑150 (MQ=60)
                                                                                            CAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAA                                                        >  SRR19973067.1096958/1‑150 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                       >  SRR19973067.205773/1‑150 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                       <  SRR19973067.1475333/150‑1 (MQ=60)
                                                                                              GCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGCGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATG                                                      <  SRR19973067.3504614/150‑1 (MQ=60)
                                                                                              GCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATG                                                      >  SRR19973067.1512884/1‑150 (MQ=60)
                                                                                              GCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATG                                                      >  SRR19973067.1512887/1‑150 (MQ=60)
                                                                                                aataattaggttattatgaccagcaaacCGCCCCGCGACCGGGTGGATACATAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAT                                                    <  SRR19973067.330510/122‑1 (MQ=60)
                                                                                                 AGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATC                                                   <  SRR19973067.523850/150‑1 (MQ=60)
                                                                                                 AGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATC                                                   >  SRR19973067.3244615/1‑150 (MQ=60)
                                                                                                 AGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATC                                                   <  SRR19973067.523974/150‑1 (MQ=60)
                                                                                                    AATACGTTCATTTGCCCAGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACT                                                <  SRR19973067.1066034/150‑1 (MQ=60)
                                                                                                           ccacagGTCCAGGCAACCGCCCCGCCACCGGGTGGATACCTAAACGCACATTAATACCACGAGCCCGCAATTTCTCAGTAATTTCCGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGcc                                         <  SRR19973067.2480771/144‑3 (MQ=60)
                                                                                                           TCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGG                                         >  SRR19973067.1945277/1‑150 (MQ=60)
                                                                                                            CATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGA                                        <  SRR19973067.2362848/150‑1 (MQ=60)
                                                                                                                   CCAGGCAAACCCCCCGCGACCGGGTGGATACCTAAACTCACATTAATACCACGAGCGCGCAATTTCTCAGTATTTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTC                                 <  SRR19973067.438846/150‑1 (MQ=60)
                                                                                                                    CAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                <  SRR19973067.150078/150‑1 (MQ=60)
                                                                                                                    CAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                <  SRR19973067.1958578/150‑1 (MQ=60)
                                                                                                                    CAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCA                                <  SRR19973067.1172297/150‑1 (MQ=60)
                                                                                                                         AAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGT                           >  SRR19973067.2134293/1‑150 (MQ=60)
                                                                                                                          AACGCCCCGCGACGGGGTGGATACCTAAACGCACATTAATACCCCGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTT                          <  SRR19973067.432725/150‑1 (MQ=60)
                                                                                                                          AACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTT                          <  SRR19973067.2700007/150‑1 (MQ=60)
                                                                                                                           ACGCCCCGCGACCGGTTGGATAAATAAACGCACATTAATACCACGATCGCGCAATTTCTAAGTATTTTAAGCGACAGTATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTC                         <  SRR19973067.1570926/150‑1 (MQ=60)
                                                                                                                            CCCCCCGCGACGGGGTGGATACATAAACGCACATTAATACCCCGAGCGCGCAATTTCACAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCC                        <  SRR19973067.1238569/150‑1 (MQ=60)
                                                                                                                               CCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCT                     <  SRR19973067.2861484/150‑1 (MQ=60)
                                                                                                                               CCCGCGACCGGGTGGATACCTAAACGCACATTAATACAACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCT                     >  SRR19973067.2297735/1‑150 (MQ=60)
                                                                                                                                 CGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGACTGGGAGTTTTTCAGCAGTTCCGCTGT                   >  SRR19973067.3082863/1‑150 (MQ=60)
                                                                                                                                 CGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGT                   <  SRR19973067.3071760/150‑1 (MQ=60)
                                                                                                                                 CGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGT                   <  SRR19973067.2953694/150‑1 (MQ=60)
                                                                                                                                   CGACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCT                 <  SRR19973067.2421799/150‑1 (MQ=60)
                                                                                                                                        GGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCT            <  SRR19973067.438298/150‑1 (MQ=60)
                                                                                                                                        GGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCT            <  SRR19973067.438384/150‑1 (MQ=60)
                                                                                                                                          GTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGC          >  SRR19973067.1903136/1‑150 (MQ=60)
                                                                                                                                           TGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCATCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCG         >  SRR19973067.1096678/1‑150 (MQ=60)
                                                                                                                                           TGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCG         <  SRR19973067.3474080/150‑1 (MQ=60)
                                                                                                                                           TGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCG         >  SRR19973067.186517/1‑150 (MQ=60)
                                                                                                                                             GATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGT       <  SRR19973067.1506188/150‑1 (MQ=60)
                                                                                                                                                 CCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTCATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATT   >  SRR19973067.1001386/1‑150 (MQ=60)
                                                                                                                                                 CCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATT   >  SRR19973067.1001384/1‑150 (MQ=60)
                                                                                                                                                 CCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATT   >  SRR19973067.3534412/1‑150 (MQ=60)
                                                                                                                                                  CTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  <  SRR19973067.824283/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT  >  CP009273/1669445‑1669740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑