Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 463,179 C→A 100% I104I (ATC→ATA)  cof → thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273463,1790CA96.2% 644.1 / 14.8 212I104I (ATC→ATA) cofthiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (3/5);  new base A (86/117);  total (89/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC  >  CP009273/463032‑463323
                                                                                                                                                   |                                                                                                                                                
  tATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATAtt                                                                                                                                                >  1:3116098/1‑148 (MQ=255)
  tATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATAtt                                                                                                                                                >  2:3385320/1‑148 (MQ=255)
    tCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCa                                                                                                                                              >  1:346600/1‑148 (MQ=255)
    tCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCa                                                                                                                                              <  1:2307629/148‑1 (MQ=255)
     cGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCaa                                                                                                                                             <  2:1940542/148‑1 (MQ=255)
          gATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATgacg                                                                                                                                        >  2:3064407/1‑148 (MQ=255)
             gCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGacgacg                                                                                                                                     <  2:1465033/148‑1 (MQ=255)
              cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACgg                                                                                                                                    <  1:808760/148‑1 (MQ=255)
              cGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACgg                                                                                                                                    <  1:2191634/148‑1 (MQ=255)
                 aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCCTGCATATATTCAATGACGACggttg                                                                                                                                 >  1:2016054/1‑148 (MQ=255)
                 aTTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttg                                                                                                                                 <  1:581084/148‑1 (MQ=255)
                  tttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACggttgg                                                                                                                                <  2:1540330/148‑1 (MQ=255)
                  tttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACggttgg                                                                                                                                <  2:1541327/148‑1 (MQ=255)
                   ttGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACggttggt                                                                                                                               <  2:2553147/148‑1 (MQ=255)
                      aTTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTa                                                                                                                            <  2:263964/148‑1 (MQ=255)
                      aTTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTa                                                                                                                            <  1:2476932/148‑1 (MQ=255)
                        tACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTAcc                                                                                                                          >  1:1871257/1‑148 (MQ=255)
                        tACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTAcc                                                                                                                          <  2:2983841/148‑1 (MQ=255)
                        tACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTAcc                                                                                                                          >  2:1770885/1‑148 (MQ=255)
                         aCCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCg                                                                                                                         <  2:2220658/148‑1 (MQ=255)
                         aCCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCg                                                                                                                         <  2:3093453/148‑1 (MQ=255)
                         aCCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCg                                                                                                                         <  1:2539006/148‑1 (MQ=255)
                            ggCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGa                                                                                                                      <  2:1572611/148‑1 (MQ=255)
                             gCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGaa                                                                                                                     >  2:1819432/1‑148 (MQ=255)
                               aaCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAag                                                                                                                   <  1:1241373/148‑1 (MQ=255)
                               aaCGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAag                                                                                                                   <  2:2105155/148‑1 (MQ=255)
                                 cGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAagag                                                                                                                 <  1:60372/148‑1 (MQ=255)
                                    aaCGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATc                                                                                                              >  1:1698376/1‑148 (MQ=255)
                                      cgcgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATccc                                                                                                            <  1:2465063/148‑1 (MQ=255)
                                      cgcgcgTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATccc                                                                                                            >  2:2730133/1‑148 (MQ=255)
                                           gTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgt                                                                                                       <  2:1645613/148‑1 (MQ=255)
                                           gTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgt                                                                                                       <  1:1153359/148‑1 (MQ=255)
                                              cATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgt                                                                                                    <  2:466016/148‑1 (MQ=255)
                                               aTTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCgttgtt                                                                                                   >  1:701378/1‑148 (MQ=255)
                                                ttCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGttgttg                                                                                                  <  1:3007816/148‑1 (MQ=255)
                                                ttCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGttgttg                                                                                                  <  2:2326417/148‑1 (MQ=255)
                                                ttCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGttgttg                                                                                                  >  1:479982/1‑148 (MQ=255)
                                                 tctcTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGc                                                                                                 >  1:146686/1‑148 (MQ=255)
                                                  ctctGGAAGGTGAACTTTTACATCGTGATGATTTACGTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGNGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCa                                                                                                >  1:1807640/1‑148 (MQ=255)
                                                    ctGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAgg                                                                                              <  1:2606965/148‑1 (MQ=255)
                                                    ctGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAgg                                                                                              <  2:2988448/148‑1 (MQ=255)
                                                     tGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGc                                                                                             <  1:2451744/148‑1 (MQ=255)
                                                          ggTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTg                                                                                        >  1:1972572/1‑148 (MQ=255)
                                                          ggTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTg                                                                                        >  2:701423/1‑148 (MQ=255)
                                                            tGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTc                                                                                      <  1:1384547/148‑1 (MQ=255)
                                                             gAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCt                                                                                     >  2:1034401/1‑148 (MQ=255)
                                                                 ttttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAg                                                                                 <  1:3327195/148‑1 (MQ=255)
                                                                   ttACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCg                                                                               <  2:42804/148‑1 (MQ=255)
                                                                    tACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCgg                                                                              <  2:1730075/148‑1 (MQ=255)
                                                                    tACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCgg                                                                              <  1:1695514/148‑1 (MQ=255)
                                                                       aTCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGttt                                                                           <  2:3443883/148‑1 (MQ=255)
                                                                        tCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGtttt                                                                          <  2:1871257/148‑1 (MQ=255)
                                                                          gTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCg                                                                        <  1:3214126/148‑1 (MQ=255)
                                                                          gTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCg                                                                        <  2:2468748/148‑1 (MQ=255)
                                                                          gTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCg                                                                        <  1:2225926/148‑1 (MQ=255)
                                                                            gatgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCAAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGtt                                                                      <  1:3520794/148‑1 (MQ=255)
                                                                             atgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTa                                                                     >  1:1632916/1‑148 (MQ=255)
                                                                             atgatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTa                                                                     <  2:3160387/148‑1 (MQ=255)
                                                                               gatTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGACAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATc                                                                   >  1:2692117/1‑148 (MQ=255)
                                                                                 tttACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAg                                                                 <  2:1310654/148‑1 (MQ=255)
                                                                                    aCCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATa                                                              <  1:49535/148‑1 (MQ=255)
                                                                                    aCCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATa                                                              <  2:3372578/148‑1 (MQ=255)
                                                                                    aCCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATa                                                              <  1:2114529/148‑1 (MQ=255)
                                                                                     ccTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                             <  2:554240/148‑1 (MQ=255)
                                                                                      cTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAAt                                                            <  2:762621/148‑1 (MQ=255)
                                                                                        gCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCg                                                          <  2:968330/148‑1 (MQ=255)
                                                                                         cGGTTGTCGCGGAGCTGGTGCTGTATGAGCAATGGGATACCCGAGCCAGCATGCATATTTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGa                                                         <  1:3561682/148‑1 (MQ=255)
                                                                                         cGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                             <  1:1212437/144‑1 (MQ=255)
                                                                                         cGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATaa                                                             >  2:1212437/1‑144 (MQ=255)
                                                                                         cGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGa                                                         <  2:2162548/148‑1 (MQ=255)
                                                                                            aTGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGt                                                      >  1:1176107/1‑148 (MQ=255)
                                                                                            aTGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGt                                                      >  1:860844/1‑148 (MQ=255)
                                                                                             tGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTc                                                     >  1:1724843/1‑148 (MQ=255)
                                                                                                    gAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCaaaaaaa                                              <  2:2313382/148‑1 (MQ=255)
                                                                                                     aGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAt                                             >  1:3127463/1‑148 (MQ=255)
                                                                                                      gCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATg                                            <  2:2413152/148‑1 (MQ=255)
                                                                                                        tGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGcc                                          <  2:610555/148‑1 (MQ=255)
                                                                                                        tGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGcc                                          >  2:1595883/1‑148 (MQ=255)
                                                                                                         ggTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCa                                         <  2:1233462/148‑1 (MQ=255)
                                                                                                          gTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCAc                                        >  2:2017885/1‑148 (MQ=255)
                                                                                                           tGCTTTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACt                                       <  1:1841897/148‑1 (MQ=255)
                                                                                                           tGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACt                                       <  1:39255/148‑1 (MQ=255)
                                                                                                               gTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGc                                   >  1:2863289/1‑148 (MQ=255)
                                                                                                                   cAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCg                               >  2:1396909/1‑148 (MQ=255)
                                                                                                                    aGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGt                              <  2:1784097/148‑1 (MQ=255)
                                                                                                                     gCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTc                             <  2:28783/148‑1 (MQ=255)
                                                                                                                      cAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCa                            <  1:122579/148‑1 (MQ=255)
                                                                                                                       aaTGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCAc                           <  2:192032/148‑1 (MQ=255)
                                                                                                                           ggATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAg                       >  2:3466772/1‑148 (MQ=255)
                                                                                                                             aTACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGAt                     >  1:2952167/1‑148 (MQ=255)
                                                                                                                              tACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATc                    <  1:1093556/148‑1 (MQ=255)
                                                                                                                                 ccGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGc                 <  2:2642721/148‑1 (MQ=255)
                                                                                                                                     gCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCt             <  1:2460117/148‑1 (MQ=255)
                                                                                                                                         gcatgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTgg         >  2:7011/1‑148 (MQ=255)
                                                                                                                                            tgcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGa      >  2:71025/1‑148 (MQ=255)
                                                                                                                                             gcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAt     >  1:2287578/1‑148 (MQ=255)
                                                                                                                                             gcatATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAt     <  1:1662072/148‑1 (MQ=255)
                                                                                                                                              catATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATCGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATc    >  2:1807474/1‑148 (MQ=255)
                                                                                                                                               atatatTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCa   >  2:2355603/1‑148 (MQ=255)
                                                                                                                                                tataTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCac  >  2:321307/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCAC  >  CP009273/463032‑463323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  >  CP009273/463031‑463327
                                                                                                                                                    |                                                                                                                                                    
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATAT                                                                                                                                                     >  SRR19973066.753003/1‑150 (MQ=60)
 GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATT                                                                                                                                                    >  SRR19973066.3137808/1‑150 (MQ=60)
 GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATT                                                                                                                                                    >  SRR19973066.3136913/1‑150 (MQ=60)
 GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATT                                                                                                                                                    <  SRR19973066.3073682/150‑1 (MQ=60)
 GCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATT                                                                                                                                                    <  SRR19973066.3073644/150‑1 (MQ=60)
  CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTC                                                                                                                                                   >  SRR19973066.1583949/1‑150 (MQ=60)
   TATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCA                                                                                                                                                  >  SRR19973066.349299/1‑150 (MQ=60)
   TATCGCTGGATGCGTATTCGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCA                                                                                                                                                  >  SRR19973066.2485583/1‑150 (MQ=60)
     TCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAAT                                                                                                                                                <  SRR19973066.520493/150‑1 (MQ=60)
     TCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAAT                                                                                                                                                <  SRR19973066.2323432/150‑1 (MQ=60)
      CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATG                                                                                                                                               <  SRR19973066.2024731/150‑1 (MQ=60)
       GCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGA                                                                                                                                              <  SRR19973066.1790555/150‑1 (MQ=60)
        CTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGAC                                                                                                                                             <  SRR19973066.1160202/150‑1 (MQ=60)
        CTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGAC                                                                                                                                             <  SRR19973066.3375964/150‑1 (MQ=60)
               CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                      <  SRR19973066.814696/150‑1 (MQ=60)
               CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                      <  SRR19973066.2206837/150‑1 (MQ=60)
               CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                      <  SRR19973066.3542888/150‑1 (MQ=60)
               CGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTT                                                                                                                                      <  SRR19973066.2206865/150‑1 (MQ=60)
                GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCCTGCATATATTCAATGACGACGGTTG                                                                                                                                     >  SRR19973066.2030174/1‑150 (MQ=60)
                GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTG                                                                                                                                     <  SRR19973066.3557855/150‑1 (MQ=60)
                GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTG                                                                                                                                     >  SRR19973066.2192685/1‑150 (MQ=60)
                  ATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGT                                                                                                                                   <  SRR19973066.585156/150‑1 (MQ=60)
                       ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                              <  SRR19973066.694428/150‑1 (MQ=60)
                       ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                              >  SRR19973066.2071814/1‑150 (MQ=60)
                       ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                              >  SRR19973066.1884619/1‑150 (MQ=60)
                       ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                              >  SRR19973066.3408076/1‑150 (MQ=60)
                       ATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACC                                                                                                                              <  SRR19973066.2493830/150‑1 (MQ=60)
                          ACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGG                                                                                                                           <  SRR19973066.2556266/150‑1 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                     >  SRR19973066.1467793/1‑150 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                     <  SRR19973066.1250190/150‑1 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                     <  SRR19973066.3036287/150‑1 (MQ=60)
                                AACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAG                                                                                                                     <  SRR19973066.1250253/150‑1 (MQ=60)
                                  CGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGAT                                                                                                                   <  SRR19973066.61119/150‑1 (MQ=60)
                                   GGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATC                                                                                                                  <  SRR19973066.1332476/150‑1 (MQ=60)
                                   GGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATC                                                                                                                  >  SRR19973066.1710719/1‑150 (MQ=60)
                                   GGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATC                                                                                                                  <  SRR19973066.1332456/150‑1 (MQ=60)
                                       CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                              <  SRR19973066.2481723/150‑1 (MQ=60)
                                       CGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTG                                                                                                              <  SRR19973066.2481870/150‑1 (MQ=60)
                                            GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                         <  SRR19973066.1161571/150‑1 (MQ=60)
                                            GTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTG                                                                                                         >  SRR19973066.732650/1‑150 (MQ=60)
                                              GCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTT                                                                                                       >  SRR19973066.706557/1‑150 (MQ=60)
                                               CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                      >  SRR19973066.1199014/1‑150 (MQ=60)
                                               CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                      >  SRR19973066.483601/1‑150 (MQ=60)
                                               CATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG                                                                                                      >  SRR19973066.1680690/1‑150 (MQ=60)
                                                ATTCTCTGGAAGGTGAACTTTTACATCTTGATGATTTACCTGCGGATGTCGCTGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC                                                                                                     >  SRR19973066.3014509/1‑150 (MQ=60)
                                                ATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCTGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC                                                                                                     >  SRR19973066.3014922/1‑150 (MQ=60)
                                                ATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC                                                                                                     >  SRR19973066.148072/1‑150 (MQ=60)
                                                 TTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACGTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGNGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCA                                                                                                    >  SRR19973066.1820482/1‑150 (MQ=60)
                                                 TTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCA                                                                                                    <  SRR19973066.3027830/150‑1 (MQ=60)
                                                  TCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAG                                                                                                   >  SRR19973066.2633818/1‑150 (MQ=60)
                                                     CTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCA                                                                                                <  SRR19973066.2624591/150‑1 (MQ=60)
                                                      TGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAT                                                                                               <  SRR19973066.2468507/150‑1 (MQ=60)
                                                      TGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCAT                                                                                               <  SRR19973066.2468456/150‑1 (MQ=60)
                                                       GGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATT                                                                                              >  SRR19973066.386081/1‑150 (MQ=60)
                                                         AAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTG                                                                                            >  SRR19973066.3346183/1‑150 (MQ=60)
                                                         AAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTG                                                                                            >  SRR19973066.1986510/1‑150 (MQ=60)
                                                             TGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTA                                                                                        <  SRR19973066.1394550/150‑1 (MQ=60)
                                                             TGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTA                                                                                        <  SRR19973066.3177843/150‑1 (MQ=60)
                                                                ACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAG                                                                                     >  SRR19973066.600649/1‑150 (MQ=60)
                                                                  TTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCG                                                                                   <  SRR19973066.3349298/150‑1 (MQ=60)
                                                                     TACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTT                                                                                <  SRR19973066.1707839/150‑1 (MQ=60)
                                                                         TCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCG                                                                            <  SRR19973066.40864/150‑1 (MQ=60)
                                                                           GTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTT                                                                          <  SRR19973066.3235412/150‑1 (MQ=60)
                                                                           GTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTT                                                                          <  SRR19973066.2241311/150‑1 (MQ=60)
                                                                            TGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCCGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA                                                                         >  SRR19973066.1644786/1‑150 (MQ=60)
                                                                             GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCAAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTAT                                                                        <  SRR19973066.3544235/150‑1 (MQ=60)
                                                                              ATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGACAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATC                                                                       >  SRR19973066.2710095/1‑150 (MQ=60)
                                                                              ATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATC                                                                       >  SRR19973066.2875488/1‑150 (MQ=60)
                                                                               TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCA                                                                      >  SRR19973066.2520869/1‑150 (MQ=60)
                                                                               TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCA                                                                      >  SRR19973066.2520516/1‑150 (MQ=60)
                                                                                    TACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAA                                                                 <  SRR19973066.497508/150‑1 (MQ=60)
                                                                                     ACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAAT                                                                <  SRR19973066.2129145/150‑1 (MQ=60)
                                                                                     ACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAAT                                                                <  SRR19973066.50172/150‑1 (MQ=60)
                                                                                      CTTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATC                                                               <  SRR19973066.1220943/150‑1 (MQ=60)
                                                                                      CCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATC                                                               <  SRR19973066.1164019/150‑1 (MQ=60)
                                                                                          CGGTTGTCGCGGAGCTGGTGCTGTATGAGCAATGGGATACCCGAGCCAGCATGCATATTTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATG                                                           <  SRR19973066.3585301/150‑1 (MQ=60)
                                                                                           GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                          >  SRR19973066.2316361/1‑150 (MQ=60)
                                                                                           GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                          >  SRR19973066.511527/1‑150 (MQ=60)
                                                                                           GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                          >  SRR19973066.1184391/1‑150 (MQ=60)
                                                                                           GGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGT                                                          >  SRR19973066.867038/1‑150 (MQ=60)
                                                                                            GATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC                                                         >  SRR19973066.1737321/1‑150 (MQ=60)
                                                                                            GATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTC                                                         >  SRR19973066.1737317/1‑150 (MQ=60)
                                                                                                    GGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAT                                                 >  SRR19973066.3148346/1‑150 (MQ=60)
                                                                                                    GGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAAT                                                 >  SRR19973066.2253671/1‑150 (MQ=60)
                                                                                                          GGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT                                           <  SRR19973066.1879138/150‑1 (MQ=60)
                                                                                                            TGCTTTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG                                         <  SRR19973066.1855062/150‑1 (MQ=60)
                                                                                                            TGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG                                         <  SRR19973066.39778/150‑1 (MQ=60)
                                                                                                              CTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGC                                       >  SRR19973066.2882553/1‑150 (MQ=60)
                                                                                                              CTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGC                                       >  SRR19973066.2882511/1‑150 (MQ=60)
                                                                                                                   TCAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGT                                  <  SRR19973066.1920950/150‑1 (MQ=60)
                                                                                                                    CAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTC                                 >  SRR19973066.2306485/1‑150 (MQ=60)
                                                                                                                    CAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTC                                 >  SRR19973066.824673/1‑150 (MQ=60)
                                                                                                                    CAGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTC                                 >  SRR19973066.92208/1‑150 (MQ=60)
                                                                                                                     AGCAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCA                                <  SRR19973066.1832398/150‑1 (MQ=60)
                                                                                                                       CAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                              <  SRR19973066.123804/150‑1 (MQ=60)
                                                                                                                       CAATGGGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                              <  SRR19973066.123813/150‑1 (MQ=60)
                                                                                                                       CAATGGGATACCCGAGCCAGCATGCATATAGTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACC                              <  SRR19973066.1917104/150‑1 (MQ=60)
                                                                                                                            GGATACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGAT                         >  SRR19973066.2971748/1‑150 (MQ=60)
                                                                                                                               TACCCGAGCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTG                      <  SRR19973066.1101517/150‑1 (MQ=60)
                                                                                                                                      GCCAGCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGT               <  SRR19973066.2476884/150‑1 (MQ=60)
                                                                                                                                          GCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCG           >  SRR19973066.1710183/1‑150 (MQ=60)
                                                                                                                                          GCATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCG           >  SRR19973066.1710158/1‑150 (MQ=60)
                                                                                                                                            ATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAATTGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAT         >  SRR19973066.2560170/1‑150 (MQ=60)
                                                                                                                                            ATGCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGAT         >  SRR19973066.2303273/1‑150 (MQ=60)
                                                                                                                                              GCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCA       <  SRR19973066.1674180/150‑1 (MQ=60)
                                                                                                                                              GCATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCA       <  SRR19973066.1674169/150‑1 (MQ=60)
                                                                                                                                                ATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACG     <  SRR19973066.2384505/150‑1 (MQ=60)
                                                                                                                                                ATATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACG     >  SRR19973066.2671472/1‑150 (MQ=60)
                                                                                                                                                 TATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGA    >  SRR19973066.3257643/1‑150 (MQ=60)
                                                                                                                                                  ATATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGAC   >  SRR19973066.3396320/1‑150 (MQ=60)
                                                                                                                                                   TATTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  >  SRR19973066.2663484/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
CGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACG  >  CP009273/463031‑463327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑