Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,669,592 G→T 100% F342L (TTC→TTA)  pntB ← pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,669,5920GT95.9% 652.5 / 17.1 220F342L (TTC→TTA) pntBpyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base T (115/96);  total (120/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 220 total aligned reads displayed.

TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGT  >  CP009273/1669447‑1669735
                                                                                                                                                 |                                                                                                                                               
tAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaa                                                                                                                                               >  1:666964/1‑148 (MQ=255)
 aGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTaaa                                                                                                                                              <  1:541844/148‑1 (MQ=255)
       aaTCCCCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGcaca                                                                                                                                        <  1:1985709/148‑1 (MQ=255)
        aTCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACAt                                                                                                                                       >  2:448267/1‑148 (MQ=255)
                  cgttatcgttatcAGAAAAGTCATAATTTATCTCGTCCATTTCCAGCACGATTTCATACGTTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACg                                                                                                                             <  1:1686987/145‑1 (MQ=255)
                  cggtatcggtatCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACg                                                                                                                             >  2:1877254/1‑148 (MQ=255)
                   ggtatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGa                                                                                                                            <  2:3299948/148‑1 (MQ=255)
                     tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgc                                                                                                                          >  2:726902/1‑148 (MQ=255)
                     tatcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgc                                                                                                                          >  1:1148395/1‑148 (MQ=255)
                      atcggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcg                                                                                                                         <  2:987525/148‑1 (MQ=255)
                        cggtatcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAgcgcg                                                                                                                       >  1:3330097/1‑148 (MQ=255)
                          gtatcAGAAAAGTCATCATTGATCTCGTCCATTTCCAGAACGATGACATCCGGTACTTTTGCTTCAGCCAGCAATACTTTCATAGGCCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCa                                                                                                                     <  1:418263/148‑1 (MQ=255)
                             tcAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAtt                                                                                                                  >  1:421270/1‑148 (MQ=255)
                              cAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAAttt                                                                                                                 <  2:1873221/148‑1 (MQ=255)
                                gCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtct                                                                                                               <  2:2938685/148‑1 (MQ=255)
                                gCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTtct                                                                                                               >  1:2687217/1‑148 (MQ=255)
                                      tcatcaTTGATCTCGTCCATTTCCATCAGGATGTCATTCTGTACTTTTGATTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTaa                                                                                                         <  1:2976979/148‑1 (MQ=255)
                                          catTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTc                                                                                                     <  1:3484784/148‑1 (MQ=255)
                                            ttGATCTCGTCCATTTCCAGCACTATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                   <  1:3396689/148‑1 (MQ=255)
                                            ttGAACTCGTCCATTGCCAGCCCGATGTCATACGGTACTTTTGCTTCAGCCCGCAATCCGTGCATAGGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAg                                                                                                   <  1:1609732/148‑1 (MQ=255)
                                              gATCTCTTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCg                                                                                                 <  2:3330779/148‑1 (MQ=255)
                                              gATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAAGACGTTCATATGTCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCCGTAATTTCAGCg                                                                                                 <  2:3360243/148‑1 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                                <  2:2647615/148‑1 (MQ=255)
                                               aTCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGa                                                                                                <  2:861349/148‑1 (MQ=255)
                                                tctcGTCCTTTTCCAGCAGGTTGTCATAGGGTACTTTTGTTTCAGCCAGCAATACTTTAATATGGCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGAc                                                                                               <  1:80115/148‑1 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                            >  1:164963/1‑148 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                            >  1:624549/1‑148 (MQ=255)
                                                   cGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAgg                                                                                            <  2:1308037/148‑1 (MQ=255)
                                                     tCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGat                                                                                          <  2:460997/148‑1 (MQ=255)
                                                      ccATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGata                                                                                         >  1:692261/1‑148 (MQ=255)
                                                        aTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAtt                                                                                       >  2:411490/1‑148 (MQ=255)
                                                        aTTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATAtt                                                                                       >  2:2573692/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                     >  1:555048/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                     >  2:3059199/1‑148 (MQ=255)
                                                          ttCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTgc                                                                                     >  1:1010002/1‑148 (MQ=255)
                                                                 aCGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcg                                                                              >  2:2684292/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                             >  2:51832/1‑148 (MQ=255)
                                                                  cGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTgcgc                                                                             >  1:74145/1‑148 (MQ=255)
                                                                    aTGTCATCCGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGa                                                                           <  2:1787009/148‑1 (MQ=255)
                                                                    aTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGa                                                                           >  2:1990139/1‑148 (MQ=255)
                                                                    aTGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGa                                                                           >  2:2492281/1‑148 (MQ=255)
                                                                       tCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTg                                                                        >  1:3122268/1‑148 (MQ=255)
                                                                        cATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGc                                                                       >  2:2129034/1‑148 (MQ=255)
                                                                        cATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGc                                                                       <  2:886335/148‑1 (MQ=255)
                                                                         aTACGGTACTTTTGCTTCAGCCACCAATACTTTCATAGGCCCAGGCAACCGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGcc                                                                      <  1:2539996/148‑1 (MQ=255)
                                                                           aCGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCAt                                                                    >  1:2653482/1‑148 (MQ=255)
                                                                            ggttatttttGATTCAGCCACCATTCGTTTCATATGTCCAGGCAAACGCCCCGCGCCGGGGTGTATACCTAAACGAACATTAATACCACGCGCGCGCAATTGCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                   <  1:2779865/142‑1 (MQ=255)
                                                                            cGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATg                                                                   <  2:405546/148‑1 (MQ=255)
                                                                             ggTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGc                                                                  <  2:3439454/148‑1 (MQ=255)
                                                                               tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCg                                                                >  2:2187870/1‑148 (MQ=255)
                                                                               tACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCg                                                                >  1:2815173/1‑148 (MQ=255)
                                                                                aCTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGt                                                               >  2:1774381/1‑148 (MQ=255)
                                                                                 cTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTa                                                              <  1:3524898/148‑1 (MQ=255)
                                                                                 cTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTa                                                              >  2:1392615/1‑148 (MQ=255)
                                                                                       cTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCgg                                                        <  2:2243921/148‑1 (MQ=255)
                                                                                         tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                      >  2:3352066/1‑148 (MQ=255)
                                                                                         tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                      >  2:1622472/1‑148 (MQ=255)
                                                                                         tCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAg                                                      <  2:601388/148‑1 (MQ=255)
                                                                                           agccagcAATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTa                                                    <  1:590434/148‑1 (MQ=255)
                                                                                             ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                  >  1:1771910/1‑148 (MQ=255)
                                                                                             ccagcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCAAATTAATACCCCGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAt                                                  <  1:1124931/148‑1 (MQ=255)
                                                                                               agcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGa                                                >  1:948505/1‑148 (MQ=255)
                                                                                                gcAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGAt                                               >  2:1318932/1‑148 (MQ=255)
                                                                                                 cAATAGTTTCATATGTCCAGCCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATc                                              <  1:3375889/148‑1 (MQ=255)
                                                                                                   aTACGTTCATATGTCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                            <  2:2544266/148‑1 (MQ=255)
                                                                                                   aTACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAc                                            >  1:916078/1‑148 (MQ=255)
                                                                                                    tACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACt                                           >  1:1154231/1‑148 (MQ=255)
                                                                                                       gtaaatttGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGaa                                        <  1:3158660/141‑1 (MQ=255)
                                                                                                         taatatGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                      <  1:1417536/146‑1 (MQ=255)
                                                                                                         tCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATg                                      >  1:3249871/1‑148 (MQ=255)
                                                                                                          cATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATgg                                     <  1:454621/148‑1 (MQ=255)
                                                                                                           atatGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATggg                                    <  2:1688798/148‑1 (MQ=255)
                                                                                                            tatGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                   >  1:1366166/1‑148 (MQ=255)
                                                                                                            tatGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                   >  2:3542130/1‑148 (MQ=255)
                                                                                                            tatGTCCAGGCAAACGCCCCGCGACCGGGTGCATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGa                                   >  2:2436788/1‑148 (MQ=255)
                                                                                                              tGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGt                                 <  1:2308631/148‑1 (MQ=255)
                                                                                                              tGTCCAGCAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGt                                 <  1:2823605/148‑1 (MQ=255)
                                                                                                               gTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                                >  2:391862/1‑148 (MQ=255)
                                                                                                               gTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                                >  2:1619067/1‑148 (MQ=255)
                                                                                                               gTCCAGCAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATCCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtt                                <  1:448267/148‑1 (MQ=255)
                                                                                                                tCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                               >  1:2031820/1‑148 (MQ=255)
                                                                                                                tCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGttt                               <  2:2852088/148‑1 (MQ=255)
                                                                                                                 ccAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                              <  2:164963/148‑1 (MQ=255)
                                                                                                                 ccAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGtttt                              <  2:3122268/148‑1 (MQ=255)
                                                                                                                   aGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGACTGGGAGTTTTTc                            >  1:2391827/1‑148 (MQ=255)
                                                                                                                   aGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTc                            >  1:2393607/1‑148 (MQ=255)
                                                                                                                     gCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcag                          >  2:2180136/1‑148 (MQ=255)
                                                                                                                       aaaCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagca                        >  1:315461/1‑148 (MQ=255)
                                                                                                                        aaCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTcagcag                       >  2:1612532/1‑148 (MQ=255)
                                                                                                                             cccGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCACTTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCg                  >  2:810171/1‑148 (MQ=255)
                                                                                                                             cccGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCg                  >  1:363963/1‑148 (MQ=255)
                                                                                                                                 cgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGt              <  1:1862634/148‑1 (MQ=255)
                                                                                                                                 cgACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGt              <  2:664928/148‑1 (MQ=255)
                                                                                                                                  gACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCGCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtc             <  2:368434/148‑1 (MQ=255)
                                                                                                                                   aCCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtct            >  2:3175611/1‑148 (MQ=255)
                                                                                                                                    ccGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGtctc           >  1:1041889/1‑148 (MQ=255)
                                                                                                                                        gTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCtcttct       <  1:2342558/148‑1 (MQ=255)
                                                                                                                                         tGGATACCTAAACGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTg      <  1:1750565/148‑1 (MQ=255)
                                                                                                                                          agATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGc     >  2:1245503/2‑148 (MQ=255)
                                                                                                                                            aTACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCgg   >  2:1566734/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGT  >  CP009273/1669447‑1669735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  CP009273/1669445‑1669741
                                                                                                                                                   |                                                                                                                                                     
GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAA                                                                                                                                                     >  SRR19973066.671837/1‑150 (MQ=60)
   AGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTAATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACG                                                                                                                                                  <  SRR19973066.545771/150‑1 (MQ=60)
      ACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCAC                                                                                                                                               >  SRR19973066.694057/1‑150 (MQ=60)
         aaacaccagaccgtTATCGTTATCACCAAAGTCATCTTTGATCTCGACCATTTCCACCACGATTTCATACGGTACTTTTGCTTCAGCCAGAAATCCGTTCATATGGCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATT                                                                                                                                            <  SRR19973066.2172826/136‑1 (MQ=60)
         AATCCCCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATT                                                                                                                                            <  SRR19973066.1999708/150‑1 (MQ=60)
          ATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTA                                                                                                                                           >  SRR19973066.923267/1‑150 (MQ=60)
               CAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACC                                                                                                                                      >  SRR19973066.2396692/1‑150 (MQ=60)
               CAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACC                                                                                                                                      >  SRR19973066.2243464/1‑150 (MQ=60)
                    cgttatggtTATCAGAAAAGTCATAATTTATATCGTCCATTTCCAGCACTATGTAATACGGTACTTTTGCTTCAGCCAGAAATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAG                                                                                                                                 <  SRR19973066.1682833/141‑1 (MQ=60)
                    cggtatggttatcagaaaagtaatattttatctcttcaATTTCCAGCACGATGTAATAGGTTATTTTTGCTTCAGCCAGAAATACGTTAATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAG                                                                                                                                 <  SRR19973066.1682866/112‑1 (MQ=60)
                    CGTTATCGTTATCAGAAAATTCATAATTGATCTCGTCCATTTCCAGCACGTTGTCATAGGGTACTTTTGTTTCAGCCAGAAATACGTTCATTTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAG                                                                                                                                 <  SRR19973066.1053093/150‑1 (MQ=60)
                    CGTTATCGTTATCAGAAAAGTCATAATTTATCTCGTCCATTTCCAGCACGATTTCATACGTTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAG                                                                                                                                 <  SRR19973066.1699267/150‑1 (MQ=60)
                     GGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGC                                                                                                                                >  SRR19973066.993436/1‑150 (MQ=60)
                     GGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGC                                                                                                                                >  SRR19973066.993359/1‑150 (MQ=60)
                     GGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGC                                                                                                                                >  SRR19973066.1155930/1‑150 (MQ=60)
                     GGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGC                                                                                                                                >  SRR19973066.1156588/1‑150 (MQ=60)
                      ttatgggtatcagaaaattcatatTTGATCTCGTCATTTTCCAGCACGATGTCATAGGTTATTTTTGCTTCAGCAAGAAATACTTTAATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCG                                                                                                                               <  SRR19973066.1643890/126‑1 (MQ=60)
                      GTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCG                                                                                                                               >  SRR19973066.204097/1‑150 (MQ=60)
                        ATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCG                                                                                                                             >  SRR19973066.3353352/1‑150 (MQ=60)
                        ATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCG                                                                                                                             >  SRR19973066.3352229/1‑150 (MQ=60)
                            GTATCAGAAAAGTCATCATTGATCTCGTCCATTTCCAGAACGATGACATCCGGTACTTTTGCTTCAGCCAGCAATACTTTCATAGGCCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAAT                                                                                                                         <  SRR19973066.421524/150‑1 (MQ=60)
                             TATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATT                                                                                                                        >  SRR19973066.424034/1‑150 (MQ=60)
                             TATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATT                                                                                                                        >  SRR19973066.424546/1‑150 (MQ=60)
                             TATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATAAGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATT                                                                                                                        >  SRR19973066.2534014/1‑150 (MQ=60)
                             TATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATAAGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATT                                                                                                                        >  SRR19973066.734325/1‑150 (MQ=60)
                              ATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTT                                                                                                                       >  SRR19973066.3542562/1‑150 (MQ=60)
                                CAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCT                                                                                                                     >  SRR19973066.2705142/1‑150 (MQ=60)
                                      AGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAA                                                                                                               >  SRR19973066.57573/1‑150 (MQ=60)
                                        taatatTTGATCTCGTCCATTTCCAGCACGATGTAATAGGGTACTTTTGCTTCCGCCACAAATACGTTAATTTGTCCCGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATT                                                                                                             <  SRR19973066.36762/144‑1 (MQ=60)
                                        TCATCATTGATCTCGTCCATTTCCATCAGGATGTCATTCTGTACTTTTGATTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATT                                                                                                             <  SRR19973066.2996652/150‑1 (MQ=60)
                                            CATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAG                                                                                                         <  SRR19973066.3508032/150‑1 (MQ=60)
                                              TTGATCTCGTCCATTTCCAGCACTATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCG                                                                                                       <  SRR19973066.3419350/150‑1 (MQ=60)
                                              TTGAACTCGTCCATTGCCAGCCCGATGTCATACGGTACTTTTGCTTCAGCCCGCAATCCGTGCATAGGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCG                                                                                                       <  SRR19973066.1621410/150‑1 (MQ=60)
                                                  TCTCGTCCTTTTCCAGCAGGTTGTCATAGGGTACTTTTGTTTCAGCCAGCAATACTTTAATATGGCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAG                                                                                                   <  SRR19973066.81032/150‑1 (MQ=60)
                                                  TCTCGTCATTTTCCAGCACTTTGTCATACGGTAGTTTTGATTCAGCCAGCATTAGGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAG                                                                                                   <  SRR19973066.80160/150‑1 (MQ=60)
                                                   CTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGG                                                                                                  >  SRR19973066.166455/1‑150 (MQ=60)
                                                   CTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGG                                                                                                  >  SRR19973066.629006/1‑150 (MQ=60)
                                                     CGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGAT                                                                                                >  SRR19973066.420196/1‑150 (MQ=60)
                                                      ttcattttccagcactttgtaataggttatttttgcttcagccacaaatccttaaatctgccctgcaaacCGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATA                                                                                               <  SRR19973066.1330433/80‑1 (MQ=60)
                                                      GTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATA                                                                                               >  SRR19973066.697363/1‑150 (MQ=60)
                                                      GTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATA                                                                                               >  SRR19973066.697300/1‑150 (MQ=60)
                                                      GTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGACAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATA                                                                                               >  SRR19973066.2621790/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973066.558991/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973066.1017454/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973066.559027/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973066.1017447/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973066.1550326/1‑150 (MQ=60)
                                                          ATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGC                                                                                           >  SRR19973066.2356753/1‑150 (MQ=60)
                                                                  CACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGC                                                                                   >  SRR19973066.2781814/1‑150 (MQ=60)
                                                                  CACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGC                                                                                   >  SRR19973066.75019/1‑150 (MQ=60)
                                                                      ATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACT                                                                               >  SRR19973066.3489213/1‑150 (MQ=60)
                                                                       TGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTG                                                                              >  SRR19973066.3143118/1‑150 (MQ=60)
                                                                           ATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCAT                                                                          >  SRR19973066.2671285/1‑150 (MQ=60)
                                                                           ATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGCCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCGGCGACAGGATATTGCGCCTGCGCGACTGCCAT                                                                          <  SRR19973066.3302163/150‑1 (MQ=60)
                                                                           ATACGGTACTTTTGCTTCAGCCACCAATACTTTCATAGGCCCAGGCAACCGCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCAT                                                                          <  SRR19973066.2557258/150‑1 (MQ=60)
                                                                            tcggttatttttgatacacccaccaatagtttcattggcccagcaaacccCCCCGCGACGGGGTGTATACCTAAACCCAAATTAATACCACGAGCGCGCATTTTCTCATTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATG                                                                         <  SRR19973066.313977/100‑1 (MQ=60)
                                                                              ggttatttttgattcagccaccattcgtTTCATATGTCCAGGCAAACGCCCCGCGCCGGGGTGTATACCTAAACGAACATTAATACCACGCGCGCGCAATTGCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCC                                                                       <  SRR19973066.2798579/122‑1 (MQ=60)
                                                                              CGGTACTTTTGCTTCCCCCAGCAATACTTGCATATGTCCCGCAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATGTCTCCGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCC                                                                       <  SRR19973066.2798357/150‑1 (MQ=60)
                                                                               GGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCG                                                                      >  SRR19973066.2832678/1‑150 (MQ=60)
                                                                               GGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCG                                                                      >  SRR19973066.2834100/1‑150 (MQ=60)
                                                                               GGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCG                                                                      >  SRR19973066.1731054/1‑150 (MQ=60)
                                                                                   CTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACC                                                                  <  SRR19973066.3548358/150‑1 (MQ=60)
                                                                                   CTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACC                                                                  >  SRR19973066.669784/1‑150 (MQ=60)
                                                                                     TTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCC                                                                >  SRR19973066.3261945/1‑150 (MQ=60)
                                                                                             cgccacaaaacCGTTCATTTGGCCAGGAAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGCGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        <  SRR19973066.167035/139‑1 (MQ=60)
                                                                                             AGCCAGCAATACTTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        <  SRR19973066.594540/150‑1 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        >  SRR19973066.1784594/1‑150 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        >  SRR19973066.1174220/1‑150 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        <  SRR19973066.594586/150‑1 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        >  SRR19973066.3557038/1‑150 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        >  SRR19973066.1201063/1‑150 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        >  SRR19973066.2697788/1‑150 (MQ=60)
                                                                                             AGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAAT                                                        >  SRR19973066.2990402/1‑150 (MQ=60)
                                                                                               CCAGCAATCCGTTCATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGA                                                      <  SRR19973066.2922274/150‑1 (MQ=60)
                                                                                               CCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGA                                                      >  SRR19973066.955432/1‑150 (MQ=60)
                                                                                               CCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCAAATTAATACCCCGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGA                                                      <  SRR19973066.1133026/150‑1 (MQ=60)
                                                                                                   aaatagtTTCATTTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  <  SRR19973066.3398593/143‑1 (MQ=60)
                                                                                                   CAATAGTTTCATATGTCCAGCCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  <  SRR19973066.3398400/150‑1 (MQ=60)
                                                                                                   CAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  >  SRR19973066.1410038/1‑150 (MQ=60)
                                                                                                   CAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCAC                                                  >  SRR19973066.922634/1‑150 (MQ=60)
                                                                                                    AATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACT                                                 >  SRR19973066.1162445/1‑150 (MQ=60)
                                                                                                    AATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACT                                                 >  SRR19973066.1162420/1‑150 (MQ=60)
                                                                                                    AATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGAAATGATCACT                                                 >  SRR19973066.3079753/1‑150 (MQ=60)
                                                                                                         gttctttggtcctGGCAAAGGCCCCGGGACGGGGTGTATACCTAAACGAACTTTAATCCCACGAGCGCGAATTTTCTCCGTATTTTCAGCGACAGGATATTGCGCCTGCTCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATG                                            <  SRR19973066.1330637/137‑1 (MQ=60)
                                                                                                         gtaaattTGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATG                                            <  SRR19973066.3179710/143‑1 (MQ=60)
                                                                                                         GTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATG                                            >  SRR19973066.3271283/1‑150 (MQ=60)
                                                                                                           TAATATGTCCAGGAAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGG                                          <  SRR19973066.1427690/150‑1 (MQ=60)
                                                                                                            CATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGA                                         >  SRR19973066.1376045/1‑150 (MQ=60)
                                                                                                            CATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGA                                         <  SRR19973066.458116/150‑1 (MQ=60)
                                                                                                            CATATGGCCAGCAAAACCCCCCGCGACCGGGTGTATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGA                                         <  SRR19973066.458019/150‑1 (MQ=60)
                                                                                                               tggccccggaaacCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTT                                      <  SRR19973066.3320276/137‑1 (MQ=60)
                                                                                                               attaactgcaaacCGCCCCGGGAGCGGGTGGATACATAACCGCACATTAATTCCACGAGCGCGCAATTTATCTGTATTTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTT                                      <  SRR19973066.3320024/137‑1 (MQ=60)
                                                                                                                TGTCCAGGCAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTT                                     <  SRR19973066.2841529/150‑1 (MQ=60)
                                                                                                                TGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTT                                     >  SRR19973066.2046006/1‑150 (MQ=60)
                                                                                                                TGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTT                                     <  SRR19973066.2324439/150‑1 (MQ=60)
                                                                                                                TGTCCAGCAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTT                                     <  SRR19973066.2842578/150‑1 (MQ=60)
                                                                                                                 ggcctGGCAAACCCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTT                                    <  SRR19973066.435320/145‑1 (MQ=60)
                                                                                                                 GTCCAGCAAACCGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATCCCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTT                                    <  SRR19973066.451723/150‑1 (MQ=60)
                                                                                                                   CCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGACTGGGAGTTTTTC                                  >  SRR19973066.2407924/1‑150 (MQ=60)
                                                                                                                   CCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGACTGGGAGTTTTTC                                  >  SRR19973066.2409552/1‑150 (MQ=60)
                                                                                                                   CCAGGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTC                                  >  SRR19973066.2409726/1‑150 (MQ=60)
                                                                                                                      GGCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGC                               <  SRR19973066.509841/150‑1 (MQ=60)
                                                                                                                       GCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCA                              >  SRR19973066.317836/1‑150 (MQ=60)
                                                                                                                       GCAAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCA                              >  SRR19973066.317814/1‑150 (MQ=60)
                                                                                                                         AAACGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGT                            <  SRR19973066.2638790/150‑1 (MQ=60)
                                                                                                                          AACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTT                           >  SRR19973066.892489/1‑150 (MQ=60)
                                                                                                                            CGCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCC                         <  SRR19973066.1972357/150‑1 (MQ=60)
                                                                                                                             GCCCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCG                        >  SRR19973066.366819/1‑150 (MQ=60)
                                                                                                                               CCCGCGACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCT                      <  SRR19973066.1773895/150‑1 (MQ=60)
                                                                                                                                   CGACCGGGTGGATACCTAACCGCACTTTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCT                  <  SRR19973066.1875946/150‑1 (MQ=60)
                                                                                                                                   CGACCGGGTGGATACCTAACCGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCT                  <  SRR19973066.3153976/150‑1 (MQ=60)
                                                                                                                                    GACCGGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTC                 >  SRR19973066.1049557/1‑150 (MQ=60)
                                                                                                                                        GGGTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCAGCTGTCTCTTCT             <  SRR19973066.1805881/150‑1 (MQ=60)
                                                                                                                                          GTGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGC           <  SRR19973066.2358506/150‑1 (MQ=60)
                                                                                                                                           TGGATACCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCG          <  SRR19973066.1763053/150‑1 (MQ=60)
                                                                                                                                           TGGATACCTAAACGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCG          <  SRR19973066.514698/150‑1 (MQ=60)
                                                                                                                                           TGGATACCTAAACGCACATTAATACCACGAGCGCGAAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCG          <  SRR19973066.1763160/150‑1 (MQ=60)
                                                                                                                                                 CCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATT    >  SRR19973066.1322791/1‑150 (MQ=60)
                                                                                                                                                 CCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATT    >  SRR19973066.3110918/1‑150 (MQ=60)
                                                                                                                                                 CCTAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATT    >  SRR19973066.2609337/1‑150 (MQ=60)
                                                                                                                                                  CGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT   >  SRR19973066.2465560/1‑150 (MQ=60)
                                                                                                                                                  CGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTT   >  SRR19973066.2466021/1‑150 (MQ=60)
                                                                                                                                                   TAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  <  SRR19973066.462127/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GTTAGCACCAATCACCAGTACGGTATCGGTATCAGCAAAGTCATCATTGATCTCGTCCATTTCCAGCACGATGTCATACGGTACTTTTGCTTCAGCCAGCAATACGTTCATATGTCCAGGCAAACGCCCCGCGACCGGGTGGATACCGAAACGCACATTAATACCACGAGCGCGCAATTTCTCAGTAATTTCAGCGACAGGATATTGCGCCTGCGCGACTGCCATGCCGTACCCCGGAGTAATGATCACTGAATGGGAGTTTTTCAGCAGTTCCGCTGTCTCTTCTGCGGTGATTTC  >  CP009273/1669445‑1669741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑