Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,836,192 T→A 100% I67I (ATA→ATT)  ynjH ← DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,836,1920TA98.0% 477.0 / ‑0.3 149I67I (ATA→ATT) ynjHDUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base A (66/80);  total (67/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 149 total aligned reads displayed.

TGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGG  >  CP009273/1836048‑1836336
                                                                                                                                                |                                                                                                                                                
tggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAtt                                                                                                                                               <  1:3422932/148‑1 (MQ=255)
       gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCgg                                                                                                                                        <  1:1614534/148‑1 (MQ=255)
          cTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCtt                                                                                                                                     <  2:1191911/148‑1 (MQ=255)
             aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTTTGGGTTTACGCGCCGCCACACCACCGGGTTTGGGGTAAGGGTTTTGTGATAACGCTGGGATTGCAGCAGAATTGCTTCGGGGTTaa                                                                                                                                  >  1:778607/1‑148 (MQ=255)
             aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCGGAATTCCTTCGGCTTTaa                                                                                                                                  <  2:3363917/148‑1 (MQ=255)
             aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                  <  2:1595654/148‑1 (MQ=255)
             aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCGTTACGCGCCGCCAGACCAGCGGTTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAATCCTTCGGCTTTaa                                                                                                                                  >  2:737476/1‑148 (MQ=255)
               cTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAtt                                                                                                                                <  1:62553/148‑1 (MQ=255)
               cTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAtt                                                                                                                                <  1:1945159/148‑1 (MQ=255)
                tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTa                                                                                                                               <  2:1030545/148‑1 (MQ=255)
                  aaGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTTCGCGCCGCCAGACCCGCGGCTTATTGCTAAGGGGTTTGTGATGACGCTTGCATTTCCGCAGAATTACATCGGCTTTTATTAca                                                                                                                             >  2:14746/1‑147 (MQ=255)
                    gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTTCCGc                                                                                                                           >  2:2846051/1‑148 (MQ=255)
                      ggCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGccc                                                                                                                         >  2:2302334/1‑148 (MQ=255)
                           tGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCa                                                                                                                    >  2:120479/1‑148 (MQ=255)
                             cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGa                                                                                                                  <  2:1727385/148‑1 (MQ=255)
                             cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCGTTAATTACCGCCCCTTCAGa                                                                                                                  >  2:3551159/1‑148 (MQ=255)
                                tatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGCGTTTCGCGCCGCCACACCCGCGGGTTAGTGCTAAGGGGTTTGTAATCACGCTGGGAGTGCAGCAGAATTCCGTAGGGTTTAATTAACGCCCCGTCAGgata                                                                                                               >  1:1550373/1‑148 (MQ=255)
                                     gCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtttt                                                                                                          <  2:787787/148‑1 (MQ=255)
                                     gCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAtttt                                                                                                          <  2:2233758/148‑1 (MQ=255)
                                      cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTg                                                                                                         <  2:2142297/148‑1 (MQ=255)
                                      cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTg                                                                                                         <  2:2142188/148‑1 (MQ=255)
                                       tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGa                                                                                                        <  2:2398258/148‑1 (MQ=255)
                                       tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGa                                                                                                        <  1:1244005/148‑1 (MQ=255)
                                         gCCCCTGCTGACATTCCATTATTAGAGGCGTGTATTGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATCACGCTGGCATTGCAGCAGAAATACTTCGGCGTTAATTAACGCCCCTTCAGAATAATTTTGATc                                                                                                      >  1:579132/1‑148 (MQ=255)
                                            ccTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTg                                                                                                   <  2:486756/148‑1 (MQ=255)
                                             ctgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGa                                                                                                  <  2:2603401/148‑1 (MQ=255)
                                              tgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGAt                                                                                                 <  1:943916/148‑1 (MQ=255)
                                                ctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaa                                                                                               <  2:947416/148‑1 (MQ=255)
                                                      ttCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgcag                                                                                         >  2:2032185/1‑148 (MQ=255)
                                                           ttattaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTTATACTGATAAAAGCAGCAATg                                                                                    >  2:3078546/1‑148 (MQ=255)
                                                              ttaGAGGCGTTTATGGGTTTACGCGCCGCCAGAACAGCGGATTATTGCAAAGGGTTTTTTCATCACGCTGGCATTGCAGCAGTATTACTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATACTTATAAAAGCAGCACAGAGt                                                                                 >  2:605761/1‑148 (MQ=255)
                                                              ttaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGt                                                                                 <  1:871586/148‑1 (MQ=255)
                                                                 gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                                                                              <  1:2310083/148‑1 (MQ=255)
                                                                 gagGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACa                                                                              <  1:2309964/148‑1 (MQ=255)
                                                                    gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATACTTTTGATCCTGATAAACGCAGCACTGAGTACAggg                                                                           >  2:591979/1‑148 (MQ=255)
                                                                    gCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAggg                                                                           >  2:651589/1‑148 (MQ=255)
                                                                       tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTg                                                                        <  1:2557413/148‑1 (MQ=255)
                                                                         tATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgcg                                                                      >  2:2258574/1‑148 (MQ=255)
                                                                         tATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTgcg                                                                      <  2:799104/148‑1 (MQ=255)
                                                                            ggCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTg                                                                   >  1:3334129/1‑148 (MQ=255)
                                                                            ggCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTg                                                                   <  2:546274/148‑1 (MQ=255)
                                                                            ggCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTg                                                                   <  1:3091045/148‑1 (MQ=255)
                                                                             gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                  <  1:184538/148‑1 (MQ=255)
                                                                              cTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                                 >  1:156974/1‑148 (MQ=255)
                                                                              cTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                                 >  2:1276679/1‑148 (MQ=255)
                                                                              cTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                                 >  1:427154/1‑148 (MQ=255)
                                                                                     cgccgcCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGc                                                          <  2:484782/148‑1 (MQ=255)
                                                                                       ccgccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCct                                                        <  2:2884160/148‑1 (MQ=255)
                                                                                        cgccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctg                                                       >  2:2130126/1‑148 (MQ=255)
                                                                                        cgccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctg                                                       >  2:1483075/1‑148 (MQ=255)
                                                                                          ccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaa                                                     >  1:930132/1‑148 (MQ=255)
                                                                                           cAGCCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaac                                                    <  1:1385237/148‑1 (MQ=255)
                                                                                           cAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaac                                                    >  1:2257298/1‑148 (MQ=255)
                                                                                           cAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaac                                                    >  1:2257583/1‑148 (MQ=255)
                                                                                           cAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaac                                                    >  1:1154045/1‑148 (MQ=255)
                                                                                             gACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactg                                                  >  1:3314559/1‑148 (MQ=255)
                                                                                                cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                               >  1:1187809/1‑148 (MQ=255)
                                                                                                cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                               >  1:1187829/1‑148 (MQ=255)
                                                                                                   cGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACt                                            >  1:2046481/1‑148 (MQ=255)
                                                                                                    ggATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTCCCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACtt                                           <  1:2049819/148‑1 (MQ=255)
                                                                                                    ggATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACtt                                           <  1:2050711/148‑1 (MQ=255)
                                                                                                     gATTTGTGCTATGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAGCGCCCCTTCAGAATAATTTTGATCCGGATAAACGCAGCACTGAGTACAGGGCTGCGGTGACTGCCCGCCTGAACTGAACACTTc                                          <  2:3064393/148‑1 (MQ=255)
                                                                                                     gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                          <  2:2097148/148‑1 (MQ=255)
                                                                                                     gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                          >  1:707556/1‑148 (MQ=255)
                                                                                                     gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                          <  2:2374157/148‑1 (MQ=255)
                                                                                                        tAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTgg                                       >  2:1457018/1‑148 (MQ=255)
                                                                                                        tAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTgg                                       >  1:1566979/1‑148 (MQ=255)
                                                                                                           tGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                    >  1:3415440/1‑148 (MQ=255)
                                                                                                             cTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTa                                  <  1:1017232/148‑1 (MQ=255)
                                                                                                               aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                >  2:3346243/1‑148 (MQ=255)
                                                                                                               aaGGGTTTTGTCATCACGCGGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTTATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                                <  1:1047348/148‑1 (MQ=255)
                                                                                                                aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                               <  1:3229742/148‑1 (MQ=255)
                                                                                                                aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGATTTAATTACCGCCCCTACAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                               <  1:1853335/148‑1 (MQ=255)
                                                                                                                 gggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGtt                              >  2:1277389/1‑148 (MQ=255)
                                                                                                                  ggTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGttt                             <  2:1413740/148‑1 (MQ=255)
                                                                                                                   gTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTa                            <  1:142730/148‑1 (MQ=255)
                                                                                                                    ttttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGTCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAc                           >  1:514995/1‑148 (MQ=255)
                                                                                                                    ttttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAc                           <  1:1501120/148‑1 (MQ=255)
                                                                                                                    ttttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAc                           <  2:2403843/148‑1 (MQ=255)
                                                                                                                     tttGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAcc                          >  2:2851598/1‑148 (MQ=255)
                                                                                                                       tGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTc                        <  2:2211171/148‑1 (MQ=255)
                                                                                                                            tcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt                   >  2:497827/1‑148 (MQ=255)
                                                                                                                             caCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAACCGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc                  <  1:771472/148‑1 (MQ=255)
                                                                                                                               cGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCg                >  1:2912219/1‑148 (MQ=255)
                                                                                                                                gCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCgg               >  2:1391392/1‑148 (MQ=255)
                                                                                                                                gCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCgg               <  2:2715519/148‑1 (MQ=255)
                                                                                                                                 cTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGa              >  2:3279849/1‑148 (MQ=255)
                                                                                                                                   ggCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACg            <  1:981045/148‑1 (MQ=255)
                                                                                                                                   ggCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACg            <  1:279111/148‑1 (MQ=255)
                                                                                                                                     cATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGAt          <  1:3211820/148‑1 (MQ=255)
                                                                                                                                     cATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGAt          >  2:2358163/1‑148 (MQ=255)
                                                                                                                                      aTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATa         <  1:79785/148‑1 (MQ=255)
                                                                                                                                       ttGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATaa        >  1:2833916/1‑148 (MQ=255)
                                                                                                                                        tGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAt       >  2:2684207/1‑148 (MQ=255)
                                                                                                                                         gcagcaGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATg      <  1:2032185/148‑1 (MQ=255)
                                                                                                                                          cagcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGc     >  1:3054747/1‑148 (MQ=255)
                                                                                                                                          cagcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGc     >  2:936706/1‑148 (MQ=255)
                                                                                                                                           agcagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCg    <  1:1724769/148‑1 (MQ=255)
                                                                                                                                             cagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCggg  >  2:1082140/1‑148 (MQ=255)
                                                                                                                                             cagAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCggg  >  2:1082125/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGG  >  CP009273/1836048‑1836336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  CP009273/1836048‑1836341
                                                                                                                                                |                                                                                                                                                     
TGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCC                                                                                                                                                  <  SRR19973066.3445824/150‑1 (MQ=60)
       GTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCT                                                                                                                                           <  SRR19973066.1626246/150‑1 (MQ=60)
       GTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCT                                                                                                                                           <  SRR19973066.3296383/150‑1 (MQ=60)
           TCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTTTGGGTTTACGCGCCGCCACACCACCGGGTTTGGGGTAAGGGTTTTGTGATAACGCTGGGATTGCAGCAGAATTGCTTCGGGGTTAA                                                                                                                                       >  SRR19973066.784358/1‑150 (MQ=60)
            CACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAT                                                                                                                                      <  SRR19973066.378496/150‑1 (MQ=60)
               CTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAC                                                                                                                                   <  SRR19973066.1958966/150‑1 (MQ=60)
               CTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAC                                                                                                                                   <  SRR19973066.63321/150‑1 (MQ=60)
               CTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAC                                                                                                                                   <  SRR19973066.1958938/150‑1 (MQ=60)
                      GGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCT                                                                                                                            <  SRR19973066.2062061/150‑1 (MQ=60)
                             CAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATAACGCTGGCATTTCAGCAGAAATACTTCGGCTTTAATTAACGCCCCGTCAGAAT                                                                                                                     >  SRR19973066.1423881/1‑150 (MQ=60)
                              AATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGCGTTTCGCGCCGCCACACCCGCGGGTTAGTGCTAAGGGGTTTGTAATCACGCTGGGAGTGCAGCAGAATTCCGTAGGGTTTAATTAACGCCCCGTCAGGATA                                                                                                                    >  SRR19973066.1561486/1‑150 (MQ=60)
                              AATATCCGCTGGCCCCTGCTGACAGTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTTCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATA                                                                                                                    <  SRR19973066.2492504/150‑1 (MQ=60)
                                       TGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATC                                                                                                           <  SRR19973066.3041325/150‑1 (MQ=60)
                                       TGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATC                                                                                                           <  SRR19973066.1252916/150‑1 (MQ=60)
                                       TGGCCCCTGCTGACATTCCATTATTAGAGGCGTGTATTGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATCACGCTGGCATTGCAGCAGAAATACTTCGGCGTTAATTAACGCCCCTTCAGAATAATTTTGATC                                                                                                           >  SRR19973066.583197/1‑150 (MQ=60)
                                           CCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGA                                                                                                       <  SRR19973066.121698/150‑1 (MQ=60)
                                            CCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCAGGCATTGCAGCAGAATTCCTTCGGCGTTAATTAACGCCCCTTCAGAATAATTTTTATCCTGAT                                                                                                      >  SRR19973066.2932027/1‑150 (MQ=60)
                                              TGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAA                                                                                                    <  SRR19973066.950803/150‑1 (MQ=60)
                                                 TGACATTCCATTATTAGAGGCGTGTATGGCTTTAAGCGCCGCCAGACCCCCGGAGTAGTGCTAAGGGTTTTTTCATCACGCGGGCAATGCCGCAGAAATCCTTCGGCGTTAATTAACGCCCCATAACAATAATTTTTATCCTGATAAAAG                                                                                                 >  SRR19973066.759884/1‑150 (MQ=58)
                                                        CCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACT                                                                                          <  SRR19973066.1189485/150‑1 (MQ=60)
                                                             ATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTA                                                                                     <  SRR19973066.2297242/150‑1 (MQ=60)
                                                              TTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTAC                                                                                    <  SRR19973066.877825/150‑1 (MQ=60)
                                                                 GAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGG                                                                                 <  SRR19973066.2325774/150‑1 (MQ=60)
                                                                 GAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGG                                                                                 <  SRR19973066.2325893/150‑1 (MQ=60)
                                                                       TTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCG                                                                           <  SRR19973066.2574774/150‑1 (MQ=60)
                                                                        TTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGC                                                                          >  SRR19973066.1647809/1‑150 (MQ=60)
                                                                          ATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTG                                                                        >  SRR19973066.3356302/1‑150 (MQ=60)
                                                                          ATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTG                                                                        >  SRR19973066.3356359/1‑150 (MQ=60)
                                                                            GGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAC                                                                      <  SRR19973066.3111695/150‑1 (MQ=60)
                                                                            GGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAC                                                                      >  SRR19973066.158420/1‑150 (MQ=60)
                                                                            GGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAC                                                                      >  SRR19973066.158369/1‑150 (MQ=60)
                                                                            GGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAC                                                                      >  SRR19973066.430461/1‑150 (MQ=60)
                                                                             GCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACT                                                                     <  SRR19973066.186253/150‑1 (MQ=60)
                                                                             GCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACT                                                                     <  SRR19973066.186148/150‑1 (MQ=60)
                                                                                        CGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAA                                                          >  SRR19973066.936877/1‑150 (MQ=60)
                                                                                        CGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAA                                                          >  SRR19973066.936896/1‑150 (MQ=60)
                                                                                         GCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAAC                                                         >  SRR19973066.2273137/1‑150 (MQ=60)
                                                                                         GCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAAC                                                         >  SRR19973066.2272851/1‑150 (MQ=60)
                                                                                         GCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAAC                                                         >  SRR19973066.1162258/1‑150 (MQ=60)
                                                                                           CAGCCCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTG                                                       <  SRR19973066.1395245/150‑1 (MQ=60)
                                                                                           CAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTG                                                       >  SRR19973066.3336562/1‑150 (MQ=60)
                                                                                              ACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAAC                                                    >  SRR19973066.1196142/1‑150 (MQ=60)
                                                                                              ACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAAC                                                    >  SRR19973066.1196162/1‑150 (MQ=60)
                                                                                                 AGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACT                                                 >  SRR19973066.2060714/1‑150 (MQ=60)
                                                                                                   CGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTC                                               >  SRR19973066.712768/1‑150 (MQ=60)
                                                                                                   CGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTC                                               >  SRR19973066.712792/1‑150 (MQ=60)
                                                                                                    GGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTCCCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCT                                              <  SRR19973066.2064067/150‑1 (MQ=60)
                                                                                                    GGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCT                                              <  SRR19973066.2064962/150‑1 (MQ=60)
                                                                                                      ATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGG                                            >  SRR19973066.1578267/1‑150 (MQ=60)
                                                                                                         AGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGG                                         >  SRR19973066.3438280/1‑150 (MQ=60)
                                                                                                             CTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACG                                     <  SRR19973066.1024718/150‑1 (MQ=60)
                                                                                                               AAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTT                                   >  SRR19973066.556665/1‑150 (MQ=60)
                                                                                                               AAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTT                                   <  SRR19973066.2264701/150‑1 (MQ=60)
                                                                                                               AAGGGTTTTGTCATCACGCGGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTTATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTT                                   <  SRR19973066.1055044/150‑1 (MQ=60)
                                                                                                                AGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTT                                  <  SRR19973066.3251080/150‑1 (MQ=60)
                                                                                                                AGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGATTTAATTACCGCCCCTACAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTT                                  <  SRR19973066.1866598/150‑1 (MQ=60)
                                                                                                                  GGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGTCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTAC                                >  SRR19973066.518795/1‑150 (MQ=60)
                                                                                                                   GTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACC                               <  SRR19973066.144103/150‑1 (MQ=60)
                                                                                                                    TTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCT                              <  SRR19973066.1511578/150‑1 (MQ=60)
                                                                                                                    TTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCT                              <  SRR19973066.1511584/150‑1 (MQ=60)
                                                                                                                             CACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAACCGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCG                     <  SRR19973066.777185/150‑1 (MQ=60)
                                                                                                                             CACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCG                     >  SRR19973066.2931654/1‑150 (MQ=60)
                                                                                                                             CACGCTGGCATTGCAGCAGAATTCCTTCGGATTTAATTCCCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCG                     <  SRR19973066.777384/150‑1 (MQ=60)
                                                                                                                                   GGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGAT               <  SRR19973066.988282/150‑1 (MQ=60)
                                                                                                                                   GGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGAT               <  SRR19973066.281240/150‑1 (MQ=60)
                                                                                                                                   GGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGAT               <  SRR19973066.281142/150‑1 (MQ=60)
                                                                                                                                     CATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAA             <  SRR19973066.3233103/150‑1 (MQ=60)
                                                                                                                                     CATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAA             <  SRR19973066.3231560/150‑1 (MQ=60)
                                                                                                                                     CATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAA             >  SRR19973066.2852963/1‑150 (MQ=60)
                                                                                                                                     CATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAA             >  SRR19973066.2337107/1‑150 (MQ=60)
                                                                                                                                      ATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAT            <  SRR19973066.80701/150‑1 (MQ=60)
                                                                                                                                        TGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGC          >  SRR19973066.3075177/1‑150 (MQ=60)
                                                                                                                                         GCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCG         <  SRR19973066.2046371/150‑1 (MQ=60)
                                                                                                                                           AGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGG       <  SRR19973066.855706/150‑1 (MQ=60)
                                                                                                                                           AGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGG       <  SRR19973066.1737247/150‑1 (MQ=60)
                                                                                                                                              AGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTA    >  SRR19973066.476051/1‑150 (MQ=60)
                                                                                                                                              AGAATTCCTTCGGATTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTA    <  SRR19973066.37961/150‑1 (MQ=60)
                                                                                                                                                AATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTAAAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  SRR19973066.3447895/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
TGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGC  >  CP009273/1836048‑1836341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑