Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,839,854 C→A 63.4% R455L (CGC→CTC)  topB ← DNA topoisomerase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,839,8540CA63.4% 58.0 / 210.1 195R455L (CGC→CTC) topBDNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (32/38);  new base A (60/64);  total (93/102)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-02

Only 100 of 201 total aligned reads displayed.

TCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAA  >  CP009273/1839711‑1840001
                                                                                                                                               |                                                                                                                                                     
 cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGctct                                                                                                                                                  >  2:1750320/1‑148 (MQ=255)
 cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCCAGctcg                                                                                                                                                  >  1:739793/1‑147 (MQ=255)
 cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTCATCGAGctct                                                                                                                                                  >  1:894303/1‑148 (MQ=255)
  ccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCtt                                                                                                                                                 <  2:1873288/148‑1 (MQ=255)
     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCAAAGCGCGCttttg                                                                                                                                              >  1:1600689/1‑146 (MQ=255)
     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTtgc                                                                                                                                              <  1:815847/148‑1 (MQ=255)
     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgc                                                                                                                                              <  2:3237722/148‑1 (MQ=255)
     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgc                                                                                                                                              <  2:3237285/148‑1 (MQ=255)
     gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGCGCTCttttc                                                                                                                                              >  1:1450311/1‑146 (MQ=255)
                  gCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGt                                                                                                                                 >  2:361184/1‑148 (MQ=255)
                   cAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGCGCTCTTTTGTGGCTAACAGCGTg                                                                                                                                >  1:147997/1‑148 (MQ=255)
                   cAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTGATCGAGCTCTGTTGTGCCGATCAGCGTg                                                                                                                                >  1:1022295/1‑148 (MQ=255)
                        ttGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCa                                                                                                                           >  2:1321670/1‑148 (MQ=255)
                            aTCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTGATCGGGGGCTTTTGTGCCTAAAAGCGTGcgcccgccg                                                                                                                       >  1:1682818/1‑147 (MQ=255)
                             tCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTg                                                                                                                      <  2:744319/148‑1 (MQ=255)
                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     >  2:145821/1‑148 (MQ=255)
                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     <  2:2932594/148‑1 (MQ=255)
                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     <  1:2223653/148‑1 (MQ=255)
                              cGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGc                                                                                                                     <  1:1126304/148‑1 (MQ=255)
                                  aaaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCa                                                                                                                 >  2:2132839/1‑148 (MQ=255)
                                      tGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCaa                                                                                                             >  1:2603241/1‑148 (MQ=255)
                                       ggCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCCGCCGGCGTCAGccac                                                                                                            >  2:642032/1‑145 (MQ=255)
                                          gcggcgGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaa                                                                                                         <  1:419403/148‑1 (MQ=255)
                                          gcggcgGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaa                                                                                                         <  1:279232/148‑1 (MQ=255)
                                            ggcggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCCCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAc                                                                                                       <  2:3304984/148‑1 (MQ=255)
                                             gcggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcg                                                                                                      >  2:1092079/1‑148 (MQ=255)
                                              cggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgc                                                                                                     <  2:608291/148‑1 (MQ=255)
                                                gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTTCTGCCTAAAAGCGTGCGCCAGCCTGCTTAAGCAAGAAAAAgcgc                                                                                                   >  1:1171040/1‑148 (MQ=255)
                                                gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAATAGAGCTATTTGATGCCGAAAACCGTGCGCCCCCCAGCTTAAGCAACAAAACacgc                                                                                                   >  1:548060/1‑148 (MQ=255)
                                                gcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTGTCTTCATCGAGCTCTTTGGTGCCTAACAGCGTGCGCCACCCAGCTTCAGCAACAAAAccccc                                                                                                   >  1:413994/1‑144 (MQ=255)
                                                 cTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                                  <  1:3461435/148‑1 (MQ=255)
                                                 cTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                                  <  2:2353574/148‑1 (MQ=255)
                                                 cTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                                  <  1:1753433/148‑1 (MQ=255)
                                                   gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCttt                                                                                                <  1:2241095/148‑1 (MQ=255)
                                                   gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTCATAGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCttt                                                                                                >  2:87784/1‑148 (MQ=255)
                                                    ggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATACCCCTCATTTCTGCCAAAAAACGTGCGCCAGCCAGCTTAAGCAAAAAAAATCGCATTa                                                                                               >  1:3056689/1‑148 (MQ=255)
                                                     gTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAg                                                                                              <  1:2541456/148‑1 (MQ=255)
                                                     gTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGCTGCCTAACAGCCAGCGGCCGCCAGCTTAACCAAGAAAAAGCGCATTat                                                                                              >  2:1210242/1‑147 (MQ=255)
                                                      tttGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGc                                                                                             >  2:173172/1‑148 (MQ=255)
                                                        tGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCCCAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGa                                                                                           <  2:580995/148‑1 (MQ=255)
                                                            gCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑Ca‑aa                                                                                     <  2:1577698/148‑1 (MQ=255)
                                                             ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AAt                                                                                    <  2:1055615/148‑1 (MQ=255)
                                                             ctctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AAt                                                                                    >  2:105788/1‑148 (MQ=255)
                                                              tctACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTAAAACAGCTCTTTTATGCCTAACAGCGTGCGCCAGCCAGCTTCAACCAGAAAACCCGCGTTAGCgcccaaat                                                                                     >  1:3438044/1‑141 (MQ=255)
                                                                  ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTgac                                                                               <  2:41015/148‑3 (MQ=255)
                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2067315/148‑1 (MQ=255)
                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2957106/148‑1 (MQ=255)
                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2873092/148‑1 (MQ=255)
                                                                   cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCt                                                                              <  1:2872891/148‑1 (MQ=255)
                                                                    aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTTCCtt                                                                             >  2:2588445/1‑148 (MQ=255)
                                                                    aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGGGCGCCCGCCTGCGTCAGCAAGAAAACGCGCTTTAGCGA‑CC‑AATTTGCCtt                                                                             >  2:3499864/1‑148 (MQ=255)
                                                                       tCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCACCCGGCTTAAGCAAGAAAAAACGCGTTAGAGACCA‑AAtttaattttg                                                                           >  1:943144/1‑141 (MQ=255)
                                                                       tCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTgg                                                                          <  2:3085919/148‑1 (MQ=255)
                                                                         aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTATCGA‑CA‑AATTTTCCGTTGGCa                                                                        >  1:2967676/1‑148 (MQ=255)
                                                                         aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGCTCATTTCTGCCGAAAAGCGTGCGCCAGCCTGCTTCAGCCAGAAAACGCGCGTTATCGA‑CA‑AAATTTCCTTTTGCa                                                                        >  1:1961219/1‑148 (MQ=255)
                                                                         aCCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTTCTGCCGAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGA‑CA‑AAATTTCCTTTggga                                                                        >  1:1494733/1‑146 (MQ=255)
                                                                           cTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTCATCGCGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCGGCTTCAGCAAGAAAAAGCGCGTTAGCGA‑CA‑AAATTTACGTTTGCAAt                                                                      >  1:1091822/1‑148 (MQ=255)
                                                                             ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGt                                                                    <  2:167954/148‑1 (MQ=255)
                                                                             ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGt                                                                    <  2:168938/148‑1 (MQ=255)
                                                                               ttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGGTGCCGAACAGCGTGCGCCCGCCGGCGTCAGCCAGAAAACGCGCTTTAGCGA‑CACAATTTCCGTTTggactgtcc                                                                   >  1:2203513/1‑141 (MQ=255)
                                                                                  acacaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGt                                                               <  2:2228460/148‑1 (MQ=255)
                                                                                   cacaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGtt                                                              <  2:664220/148‑1 (MQ=255)
                                                                                        cAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATa                                                         <  1:1256876/148‑1 (MQ=255)
                                                                                          aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAac                                                       <  2:1085958/148‑1 (MQ=255)
                                                                                              aTCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAacacac                                                   <  1:3236916/148‑1 (MQ=255)
                                                                                                cGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACtt                                                 <  2:112636/148‑1 (MQ=255)
                                                                                                 gCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTg                                                <  1:2664371/148‑1 (MQ=255)
                                                                                                  ccTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGc                                               <  1:3540694/148‑1 (MQ=255)
                                                                                                    tttCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCgg                                             >  2:2877507/1‑148 (MQ=255)
                                                                                                      tCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGaa                                           <  1:3152860/148‑1 (MQ=255)
                                                                                                        gCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCAACGCGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCGTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTGGATAAAACACATTCGGAaca                                         >  2:929013/1‑148 (MQ=255)
                                                                                                        gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAaca                                         <  1:343271/148‑1 (MQ=255)
                                                                                                            ccacAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGCTGCCTAAAAGCCTGCGCCAGCCGGCGTCAGCAAGAAAACCCCCTTTAGCGA‑CA‑AAATTTCCATTGGCCATGTGCAGTTCGATAACACACTTTCGGAACAcccc                                     >  1:3257965/1‑146 (MQ=255)
                                                                                                             cacaGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCa                                    <  1:490731/148‑1 (MQ=255)
                                                                                                             cacaGGCAGTGGCGTGCCGTCGTGTTCTTAATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCCGCCTGCTTCAGCCAGAAAACGCGCGTTAGCGA‑CA‑AATTTGCCTTTGGCCATGTCCAGTTCGATAACACACTTGCGGAACACCCCa                                    >  2:187702/1‑148 (MQ=255)
                                                                                                                aGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATcc                                 <  1:3114969/148‑1 (MQ=255)
                                                                                                                aGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCATTGGCAATGTCCAGTTCGATAACACACATTCGGAACACCGCATcc                                 >  2:586362/1‑148 (MQ=255)
                                                                                                                 ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                <  1:2132494/148‑1 (MQ=255)
                                                                                                                 ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                <  2:3696/148‑1 (MQ=255)
                                                                                                                 ggCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                <  1:610417/148‑1 (MQ=255)
                                                                                                                    aGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGc                             <  2:1082788/148‑1 (MQ=255)
                                                                                                                      tGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑ACTTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAg                           <  2:2596033/148‑1 (MQ=255)
                                                                                                                             ccGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCa                    <  2:2667969/148‑1 (MQ=255)
                                                                                                                               gtcgtTTTCTTCATAGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTTCGGAACACCGCATCCGGGCAGAATTGCATc                  >  1:3424620/1‑148 (MQ=255)
                                                                                                                                tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa                 >  2:77676/1‑148 (MQ=255)
                                                                                                                                tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCa                 >  2:2470052/1‑148 (MQ=255)
                                                                                                                                     ttcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATAc            <  2:2759756/148‑1 (MQ=255)
                                                                                                                                      tcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACt           >  2:707341/1‑148 (MQ=255)
                                                                                                                                       cttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTg          <  2:312214/148‑1 (MQ=255)
                                                                                                                                       cttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTg          <  1:173587/148‑1 (MQ=255)
                                                                                                                                       cttcATCGAGCTCTTTGCTGCCTAACTGAGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACGGCATCCGGGCAGAATTGCATCAGATTCTg          <  2:1961520/148‑1 (MQ=255)
                                                                                                                                        ttcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGa         <  1:642032/148‑1 (MQ=255)
                                                                                                                                         tcATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc        >  1:498165/1‑148 (MQ=255)
                                                                                                                                         tcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc        >  2:3358035/1‑148 (MQ=255)
                                                                                                                                         tcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAc        >  1:1294351/1‑148 (MQ=255)
                                                                                                                                          cATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg       <  2:1450311/148‑1 (MQ=255)
                                                                                                                                            tCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACggg     >  2:1158884/1‑148 (MQ=255)
                                                                                                                                               cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCaa  <  1:2913937/148‑1 (MQ=255)
                                                                                                                                               cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCaa  <  1:145821/148‑1 (MQ=255)
                                                                                                                                               cgcTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCaa  >  2:2715208/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
TCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGA‑CA‑AATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAA  >  CP009273/1839711‑1840001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATC  >  CP009273/1839710‑1840003
                                                                                                                                                    |                                                                                                                                                       
ATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCT                                                                                                                                                    >  SRR19973066.248622/1‑150 (MQ=60)
ATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCT                                                                                                                                                    >  SRR19973066.1492005/1‑150 (MQ=60)
ATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATT‑‑‑‑CATCGAGCTCT                                                                                                                                                    >  SRR19973066.900631/1‑150 (MQ=60)
 TCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCAGTCGTTTTCTT‑‑‑‑CATCGAGCTCTT                                                                                                                                                   <  SRR19973066.2865615/150‑1 (MQ=60)
   CCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTG                                                                                                                                                 <  SRR19973066.157769/150‑1 (MQ=60)
    CGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑AATCGCGCTCTTTTC                                                                                                                                                >  SRR19973066.1460606/1‑150 (MQ=60)
      GTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTG                                                                                                                                              <  SRR19973066.821824/150‑1 (MQ=60)
        CATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTT‑‑‑‑AATCGAGCTCGTTGCTGCC                                                                                                                                            >  SRR19973066.1630376/1‑150 (MQ=60)
                  AGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTT‑‑‑‑CATCGCGCTCTTTTGTGGCTAACAGCGTG                                                                                                                                  >  SRR19973066.149392/1‑150 (MQ=60)
                  AGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTT‑‑‑‑CATCGCGCTCTTTTCTTCCGAACAGCGGG                                                                                                                                  >  SRR19973066.149365/1‑150 (MQ=60)
                  AGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTT‑‑‑‑GATAGCGCGCGTTGCTGCCGAACAGCGTG                                                                                                                                  >  SRR19973066.1061308/1‑150 (MQ=60)
                      GTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTT‑‑‑‑AATCGCCCTCTTTTGTGCCTAACACCGTGCGCC                                                                                                                              >  SRR19973066.3199898/1‑150 (MQ=60)
                               CGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTT                                                                                                                     <  SRR19973066.2239023/150‑1 (MQ=60)
                               CGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTT                                                                                                                     <  SRR19973066.1134401/150‑1 (MQ=60)
                                GGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCCCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAAAACCGTGCGCCccccggcgtc                                                                                                                    >  SRR19973066.438303/1‑140 (MQ=60)
                                     AATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAA                                                                                                               >  SRR19973066.2620847/1‑150 (MQ=60)
                                      ATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAG                                                                                                              <  SRR19973066.3258761/150‑1 (MQ=60)
                                           GCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAC                                                                                                         <  SRR19973066.422671/150‑1 (MQ=60)
                                           GCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAC                                                                                                         <  SRR19973066.418736/150‑1 (MQ=60)
                                           GCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAC                                                                                                         <  SRR19973066.281264/150‑1 (MQ=60)
                                               CGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTTCTGCCTAAAAGCGTGCGCCAGCCTGCTTAAGCAAGAAAAAGCGC                                                                                                     >  SRR19973066.1179310/1‑150 (MQ=60)
                                               CGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTGTCTT‑‑‑‑CATCGAGCTCTTTGGTGCCTAACAGCGTGCGCCACCCAGCTTCAGCAACAAAACcccc                                                                                                     >  SRR19973066.417227/1‑146 (MQ=60)
                                                  CTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTT                                                                                                  <  SRR19973066.3484589/150‑1 (MQ=60)
                                                  CTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTT                                                                                                  <  SRR19973066.3484577/150‑1 (MQ=60)
                                                  CTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑AATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTT                                                                                                  <  SRR19973066.1766041/150‑1 (MQ=60)
                                                    GGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTNG                                                                                                <  SRR19973066.2256551/150‑1 (MQ=60)
                                                      GTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCG                                                                                              <  SRR19973066.2558725/150‑1 (MQ=60)
                                                             GCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTAAAACA‑‑‑‑GCTCTTTTATGCCTAACAGCGTGCGCCAGCCAGCTTCAACCAGAAAACCCGCGTTAGCGcccaaat                                                                                       >  SRR19973066.3461044/1‑143 (MQ=60)
                                                              CTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTT                                                                                     <  SRR19973066.3123160/150‑1 (MQ=60)
                                                               TCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCGCCACAGGCAGTGGCGTGCCGTCGTGTTCTT‑‑‑‑AAACGCCCGCTTTTCTTCCTAACAGCGTGCGCCccccagcataatccaaaaaaaccccggtagcgccaaaattt                                                                                     >  SRR19973066.647954/1‑109 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT                                                                               <  SRR19973066.2976704/150‑1 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT                                                                               <  SRR19973066.2892156/150‑1 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT                                                                               <  SRR19973066.2892215/150‑1 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT                                                                               <  SRR19973066.2892357/150‑1 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT                                                                               <  SRR19973066.2892359/150‑1 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT                                                                               <  SRR19973066.2976731/150‑1 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT                                                                               <  SRR19973066.2081628/150‑1 (MQ=60)
                                                                    CACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATT‑‑‑‑AATAGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCAAGAAAAAGCGCTTTATCGACAAAatttccatt                                                                                >  SRR19973066.583594/1‑141 (MQ=60)
                                                                      CTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTT‑‑‑‑AATCGAGCTCTTTTCTGCCTAACAGCGTGCGCCACCCGGCTTAAGCAAGAAAAaacgcgttagagaccaaatttaattttg                                                                              >  SRR19973066.950024/1‑122 (MQ=60)
                                                                        TCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTTTT‑‑‑‑AATCGAGCTCTTTTCTGCCTAAAAGCGTGCGCCCGCCTGCTTAAGCAAGAAAAAGCGCTTTttcgacaaaatttacttttgga                                                                            >  SRR19973066.1975025/1‑128 (MQ=60)
                                                                        TCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTATCGACAAA‑TTTTCCGTT‑GGCA                                                                          >  SRR19973066.2987302/1‑150 (MQ=60)
                                                                        TCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATAGAGCTCATTTCTGCCGAAAAGCGTGCGCCAGCCTGCTTCAGCCAGAAAACGCGCGTTATCGACAAA‑ATTTCCTTT‑TGCA                                                                          >  SRR19973066.1975084/1‑150 (MQ=60)
                                                                        TCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTT‑‑‑‑CATCGAGCTCTTTTCTGCCGAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGACAAA‑ATTTCCTTT‑GGGA                                                                          >  SRR19973066.1505172/1‑150 (MQ=60)
                                                                          ACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAAT                                                                        <  SRR19973066.799026/150‑1 (MQ=60)
                                                                          ACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTT‑‑‑‑CATCGCGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCGGCTTCAGCAAGAAAAAGCGCGTTAGCGACAAAatttacgtttgcaat                                                                          >  SRR19973066.1099772/1‑135 (MQ=60)
                                                                             TTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTC                                                                     <  SRR19973066.647276/150‑1 (MQ=60)
                                                                              TTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTT‑‑‑‑CATCGAGCTCTTTTCTGCCGAAAAGCGTGCGCCAGCCGGCGTCAGCAACAAAAAGCGCTTTATCGACAAA‑ATTTCCTTT‑GGCAAAGTCC                                                                    >  SRR19973066.2579772/1‑150 (MQ=60)
                                                                              TTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑AATAGAGCTCTTTTGTGGCTAAAAACGTGCGCCAGCCGGCTTCAGCAAGAAAACGCGCTTTAGCGCCAAA‑ATTGGCTTT‑GGGAATGTGC                                                                    >  SRR19973066.2218834/1‑150 (MQ=60)
                                                                              TTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTT‑‑‑‑GATCGGGCGCGTTGCTGCCTAACAGCGGGCGCCCGCCAGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAatttccgttgggcctgtcc                                                                      >  SRR19973066.2218880/1‑131 (MQ=60)
                                                                              TTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTT‑‑‑‑CATCGAGCTCTTTGGTGCCGAACAGCGTGCGCCCGCCGGCGTCAGCCAGAAAACGCGCTTTAGCGACAcaatttccgtttggactgtcc                                                                      >  SRR19973066.2218784/1‑129 (MQ=60)
                                                                                   ACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTC                                                               <  SRR19973066.36683/150‑1 (MQ=60)
                                                                                    CACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CAACCAGCTCTTTGCTTCCGAACAGCGTGCGCCAGCCAGCCTCAGCAAGAAAACCCGCTGTATCGACAAAaattactgtggccatgtcccgttag                                                                >  SRR19973066.2623965/1‑125 (MQ=60)
                                                                                    CACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTT‑‑‑‑CATCGAGCTCTTTGCTGGCGAACAGCGGGCGCCAGCCGGCGTCAGCAAGAAAAAGCGCGTTAGCGACAAATTTTGCGTTT‑GG‑AAAGTCCAGTTCG                                                              >  SRR19973066.822821/1‑150 (MQ=60)
                                                                                       AGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTT‑‑‑‑GATCGAGCTCTTTGGTGGCGAAAAGCGTGCGCCAGCCGGCTTCAGCAAGAAAACGCGCGTTAGCGACAAA‑TTTTCCTTT‑GGCAATGTCCAGTTCGATT                                                           >  SRR19973066.2240651/1‑150 (MQ=60)
                                                                                         CAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAAC                                                         <  SRR19973066.3053639/150‑1 (MQ=60)
                                                                                         CAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAAC                                                         <  SRR19973066.1265935/150‑1 (MQ=60)
                                                                                           ACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACAC                                                       <  SRR19973066.968200/150‑1 (MQ=60)
                                                                                             TCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTT‑‑‑‑AATAGCGCTCTTTTCTGCCGAACAGCGTGCGCCAGCCGGCTTCAGCAAGAAAAAGCGCTTTATCGACAAA‑ATTGGCTTT‑GGGAATGTCCAGGTCGATAACACAC                                                     >  SRR19973066.499588/1‑150 (MQ=60)
                                                                                             TCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACAC                                                     <  SRR19973066.2140085/150‑1 (MQ=60)
                                                                                             TCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTGTGTT‑‑‑‑AAACGAGCTCTTTTGTGCCGAACAGCGTGCGCCCGCCGGCTTCAGCAAcaaaaaccgcgttatcgacaaaatttccttttggaatttccaggtcgctaaaacac                                                       >  SRR19973066.2839369/1‑94 (MQ=42)
                                                                                               ATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTT                                                   <  SRR19973066.2933835/150‑1 (MQ=60)
                                                                                               ATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTT                                                   <  SRR19973066.3258284/150‑1 (MQ=60)
                                                                                                  GCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCG                                                <  SRR19973066.2682211/150‑1 (MQ=60)
                                                                                                  GCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCG                                                <  SRR19973066.2739241/150‑1 (MQ=60)
                                                                                                   CCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGG                                               <  SRR19973066.3564217/150‑1 (MQ=60)
                                                                                                     TTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTGTCTT‑‑‑‑GAACGAGCTCTTTGGTGGCTAAAAACGTGCGCCCGCCCGCTTGATCAAGAAAAAGCGCCTTAGCGACAAA‑TTTTCCTTT‑GGCAAagtgcccgtcgtgaaaacacatgcggga                                             >  SRR19973066.1288108/1‑122 (MQ=39)
                                                                                                       TCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACA                                           <  SRR19973066.3173880/150‑1 (MQ=60)
                                                                                                       TCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACA                                           <  SRR19973066.3171509/150‑1 (MQ=60)
                                                                                                         GCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACC                                         <  SRR19973066.345929/150‑1 (MQ=60)
                                                                                                         GCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACC                                         <  SRR19973066.345933/150‑1 (MQ=60)
                                                                                                           CACCACAGGCAGTGGCGTGCCGTCGTGTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAAAAGCCTGCGCCAGCCGGCGTCAGCAAGAAAACCCCCTTTAGCGACAAA‑ATTTCCATT‑GGCCATGTGCAGTTCGATAACACACTTTCGGAACACCCC                                       >  SRR19973066.3279398/1‑150 (MQ=60)
                                                                                                            ACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑AATCGAGCGGGTTGGTGCCTAACACCGGGCGGCCGCCAGCTTAAGCAAGAAAACGCGCGTTATCGACAAA‑ATTGGCTTTGGGCAAT‑TCCAGTTCGTTAACACACTTTCGGAACACCCCA                                      >  SRR19973066.1405924/1‑150 (MQ=27)
                                                                                                            ACCACAGGCAGTGGCGTGCCGTCGTGTTGTT‑‑‑‑GATCGAGCTCTTTTCTGCCTAAAAGCGTGCGCCAGCCGGCTTGAGCAAAAAAAAGCGCGTTTGCGACAAA‑TTTTGCATT‑TGCAATTTGCAGTTAGATAACACACATGCGGAACACCCCA                                      >  SRR19973066.1405926/1‑150 (MQ=33)
                                                                                                              CACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATC                                    <  SRR19973066.1460148/150‑1 (MQ=60)
                                                                                                              CACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATC                                    <  SRR19973066.494407/150‑1 (MQ=60)
                                                                                                              CACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATC                                    <  SRR19973066.492676/150‑1 (MQ=60)
                                                                                                                 AGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGG                                 <  SRR19973066.3135773/150‑1 (MQ=60)
                                                                                                                  GGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGG                                <  SRR19973066.2147270/150‑1 (MQ=60)
                                                                                                                  GGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGG                                <  SRR19973066.614671/150‑1 (MQ=60)
                                                                                                                         GCGTGCCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATT                         <  SRR19973066.2147622/150‑1 (MQ=60)
                                                                                                                             GCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCAT                     <  SRR19973066.1762913/150‑1 (MQ=60)
                                                                                                                              CCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATC                    >  SRR19973066.3447568/1‑150 (MQ=60)
                                                                                                                              CCGTCGTTTTCTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATC                    <  SRR19973066.1196380/150‑1 (MQ=60)
                                                                                                                              CCGTCGTTTTCTT‑‑‑‑CATAGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAAGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTTCGGAACACCGCATCCGGGCAGAATTGCATC                    >  SRR19973066.3447523/1‑150 (MQ=60)
                                                                                                                                        CTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC          >  SRR19973066.1125921/1‑150 (MQ=60)
                                                                                                                                        CTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC          <  SRR19973066.174711/150‑1 (MQ=60)
                                                                                                                                        CTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC          >  SRR19973066.501872/1‑150 (MQ=60)
                                                                                                                                        CTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC          >  SRR19973066.502109/1‑150 (MQ=60)
                                                                                                                                        CTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC          <  SRR19973066.175131/150‑1 (MQ=60)
                                                                                                                                        CTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC          >  SRR19973066.1303729/1‑150 (MQ=60)
                                                                                                                                        CTT‑‑‑‑CATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGAC          >  SRR19973066.1303706/1‑150 (MQ=60)
                                                                                                                                         TT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACG         <  SRR19973066.646674/150‑1 (MQ=60)
                                                                                                                                         TT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACG         <  SRR19973066.646570/150‑1 (MQ=60)
                                                                                                                                                 TCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCA     <  SRR19973066.908384/150‑1 (MQ=60)
                                                                                                                                                   GAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT   <  SRR19973066.996326/150‑1 (MQ=60)
                                                                                                                                                   GAGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT   <  SRR19973066.1218708/150‑1 (MQ=60)
                                                                                                                                                    CGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATC  <  SRR19973066.147172/150‑1 (MQ=60)
                                                                                                                                                    CGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATC  <  SRR19973066.147205/150‑1 (MQ=60)
                                                                                                                                                    CGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATC  <  SRR19973066.2933379/150‑1 (MQ=60)
                                                                                                                                                    AGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATC  <  SRR19973066.565043/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                       
ATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTT‑‑‑‑CATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAA‑TTTGCCTTT‑GGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAATC  >  CP009273/1839710‑1840003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑