Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,855,239 A→T 36.6% N117K (AAT→AAA)  ydjL ← putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,855,2390AT36.6% 185.0 / 223.1 200N117K (AAT→AAA) ydjLputative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (60/66);  new base T (35/38);  total (96/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

Only 100 of 213 total aligned reads displayed.

ATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACC  >  CP009273/1855093‑1855383
                                                                                                                                                  |                                                                                                                                                
aTGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtat                                                                                                                                                 >  2:196142/1‑148 (MQ=255)
  ggATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtttta                                                                                                                                               <  1:2211633/148‑1 (MQ=255)
  ggATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtatta                                                                                                                                               >  1:453583/1‑148 (MQ=255)
   gATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGttttat                                                                                                                                              <  1:1412041/148‑1 (MQ=255)
   gATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGtattat                                                                                                                                              >  2:362468/1‑148 (MQ=255)
       tGTAGTCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACAACATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATc                                                                                                                                          <  2:3380353/148‑1 (MQ=255)
            gCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGAc                                                                                                                                     >  2:185906/1‑148 (MQ=255)
              aTTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCa                                                                                                                                   <  1:2925525/148‑1 (MQ=255)
               ttATAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCaa                                                                                                                                  <  2:1469894/148‑1 (MQ=255)
                  cAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGt                                                                                                                               <  1:3369647/148‑1 (MQ=255)
                    gATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGttt                                                                                                                             <  2:2802003/148‑1 (MQ=255)
                     attgggTCAAGTACGGCTGCGTCCTAATAATAAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTa                                                                                                                            <  1:1837230/145‑1 (MQ=255)
                     aTAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTa                                                                                                                            <  2:2717535/148‑1 (MQ=255)
                      tAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTAc                                                                                                                           <  1:3178419/148‑1 (MQ=255)
                       aGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACt                                                                                                                          >  2:1228394/1‑148 (MQ=255)
                        gggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACtt                                                                                                                         >  1:2100350/1‑148 (MQ=255)
                        gggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACtt                                                                                                                         >  1:3554467/1‑148 (MQ=255)
                        gggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACtt                                                                                                                         >  2:3035136/1‑148 (MQ=255)
                           tCAAGTACGGCGGCGTCCTCAGAATCAACCCCCTCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACttttt                                                                                                                      <  2:3562733/148‑1 (MQ=255)
                            cAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTc                                                                                                                     >  1:1719196/1‑148 (MQ=255)
                            cAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTc                                                                                                                     >  1:2017883/1‑148 (MQ=255)
                             aaGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCt                                                                                                                    <  1:3483896/148‑1 (MQ=255)
                             aaGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCt                                                                                                                    >  1:1020826/1‑148 (MQ=255)
                             aaGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCt                                                                                                                    <  1:2867719/148‑1 (MQ=255)
                                    gCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTacaac                                                                                                             >  1:1417766/1‑148 (MQ=255)
                                     cTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTacaaca                                                                                                            >  1:2103438/1‑148 (MQ=255)
                                      tGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAacac                                                                                                           <  1:1765659/148‑1 (MQ=255)
                                           ccTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGaaa                                                                                                      >  2:3238200/1‑148 (MQ=255)
                                           ccTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAAGTTTACTTTTTCTGTACAACACAGaaa                                                                                                      <  1:2961915/148‑1 (MQ=255)
                                           ccTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAAGTTTACTTTTTCTGTACAACACAGaaa                                                                                                      <  1:2962367/148‑1 (MQ=255)
                                           ccTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTCTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGaaa                                                                                                      >  1:3312220/1‑148 (MQ=255)
                                           ccTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGaaa                                                                                                      <  2:2573018/148‑1 (MQ=255)
                                             tCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAAt                                                                                                    <  1:2205462/148‑1 (MQ=255)
                                             acATAATCAACACCATCAGGGATTTCCCACACCGCCTGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACCACACAGAAAAt                                                                                                    <  2:2443526/147‑1 (MQ=255)
                                              cATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATc                                                                                                   >  2:1359211/1‑148 (MQ=255)
                                              cATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAAGTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATc                                                                                                   <  2:2146690/148‑1 (MQ=255)
                                               aTAATCAACACCATCATGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCa                                                                                                  <  2:2928771/148‑1 (MQ=255)
                                                tAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAc                                                                                                 >  2:312050/1‑148 (MQ=255)
                                                tAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAc                                                                                                 >  1:662146/1‑148 (MQ=255)
                                                tAATCAACACCATCAGGGATGTCCCACAACGCATGACGATGAATTTTGTGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAc                                                                                                 <  2:1871136/148‑1 (MQ=255)
                                                 aaTCAACACCATCAGGGATTTCCCACCACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCAcc                                                                                                <  1:1072203/148‑1 (MQ=255)
                                                       cacCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTc                                                                                          <  1:2788407/148‑1 (MQ=255)
                                                        acCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTca                                                                                         >  1:902583/1‑148 (MQ=255)
                                                         ccATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTcac                                                                                        <  1:1228394/148‑1 (MQ=255)
                                                          cATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTcaca                                                                                       <  2:227553/148‑1 (MQ=255)
                                                          cATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTcaca                                                                                       <  1:2915893/148‑1 (MQ=255)
                                                          cATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTcaca                                                                                       <  2:684472/148‑1 (MQ=255)
                                                           aTCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAg                                                                                      >  1:66515/1‑148 (MQ=255)
                                                              aGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGcc                                                                                   <  1:185906/148‑1 (MQ=255)
                                                              aGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGcc                                                                                   <  1:1348352/148‑1 (MQ=255)
                                                                ggATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCgg                                                                                 <  2:824528/148‑1 (MQ=255)
                                                                    ttCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACaa                                                                             >  1:1382792/1‑148 (MQ=255)
                                                                     tCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACaaa                                                                            <  2:3537941/148‑1 (MQ=255)
                                                                       ccACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAaca                                                                          >  2:2833596/1‑148 (MQ=255)
                                                                         acaACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACAcc                                                                        >  1:809426/1‑148 (MQ=255)
                                                                             cGCATGACGATGTATTTTGATAATTTCACCAGGAACCAGAAAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCaa                                                                    <  1:2033181/148‑1 (MQ=255)
                                                                             cGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCaa                                                                    <  1:235353/148‑1 (MQ=255)
                                                                               cATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAAc                                                                  >  1:2862668/1‑148 (MQ=255)
                                                                                aTGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACg                                                                 <  2:1711679/148‑1 (MQ=255)
                                                                                  gACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTg                                                               >  2:1178416/1‑148 (MQ=255)
                                                                                  gACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTg                                                               <  2:2263251/148‑1 (MQ=255)
                                                                                  gACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTg                                                               >  1:2589054/1‑148 (MQ=255)
                                                                                  gACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCATGTTCACAGGCCGGACAAACACCGCAAACGTg                                                               >  2:2037161/1‑148 (MQ=255)
                                                                                      aTGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCg                                                           <  1:1031976/148‑1 (MQ=255)
                                                                                      aTGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCg                                                           >  2:3430367/1‑148 (MQ=255)
                                                                                       tGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGc                                                          <  2:1098331/148‑1 (MQ=255)
                                                                                       tGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGc                                                          >  1:2952768/1‑148 (MQ=255)
                                                                                       tGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGc                                                          >  1:843954/1‑148 (MQ=255)
                                                                                         aaTTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTg                                                        <  2:330768/148‑1 (MQ=255)
                                                                                            tttGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTa                                                     >  2:3466367/1‑148 (MQ=255)
                                                                                                  aaTTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTNTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAt                                               <  1:1805985/148‑1 (MQ=255)
                                                                                                     ttCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATacg                                            >  1:1922253/1‑148 (MQ=255)
                                                                                                     ttCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATacg                                            >  1:2703992/1‑148 (MQ=255)
                                                                                                         ccAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGAcgc                                        >  2:263122/1‑148 (MQ=255)
                                                                                                          cAGGAACCAGACAATATTAGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGAcgcg                                       >  1:384981/1‑148 (MQ=255)
                                                                                                           aGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGt                                      <  1:584677/148‑1 (MQ=255)
                                                                                                            ggAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACTGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGtt                                     >  2:545396/1‑148 (MQ=255)
                                                                                                            ggAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGtt                                     >  2:2296325/1‑148 (MQ=255)
                                                                                                             gAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTg                                    >  1:2272076/1‑148 (MQ=255)
                                                                                                               aCCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGcc                                  <  1:362468/148‑1 (MQ=255)
                                                                                                               aCCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGcc                                  >  1:949712/1‑148 (MQ=255)
                                                                                                                  aGACAATATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCa                               <  1:3238200/148‑1 (MQ=255)
                                                                                                                     cAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACtt                            >  1:2452896/1‑148 (MQ=255)
                                                                                                                      aaTATTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACttt                           <  2:2548781/148‑1 (MQ=255)
                                                                                                                      aaTATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACttt                           >  2:2019709/1‑148 (MQ=255)
                                                                                                                         atTTGGAAAAACCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCa                        >  2:499496/1‑148 (MQ=255)
                                                                                                                           ttGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGt                      >  1:1552239/1‑148 (MQ=255)
                                                                                                                           gtGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGt                      <  2:3046388/147‑1 (MQ=255)
                                                                                                                             ggAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCt                    <  1:833145/148‑1 (MQ=255)
                                                                                                                                 aaaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGa                <  1:841504/148‑1 (MQ=255)
                                                                                                                                 aaaCCACCGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGa                <  1:2628615/148‑1 (MQ=255)
                                                                                                                                 aaaCCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGa                <  2:2317691/148‑1 (MQ=255)
                                                                                                                                   accaccGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACt              >  1:2671689/1‑148 (MQ=255)
                                                                                                                                    ccaccGCCCCAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtt             <  1:1988982/148‑1 (MQ=255)
                                                                                                                                      accGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtttt           >  2:2456382/1‑148 (MQ=255)
                                                                                                                                      accGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACtttt           <  2:1189429/148‑1 (MQ=255)
                                                                                                                                         gCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCa        <  1:2601978/148‑1 (MQ=255)
                                                                                                                                            ccAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCa     >  2:3106997/1‑148 (MQ=255)
                                                                                                                                             cAGGTTTTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCaa    >  2:2884044/1‑148 (MQ=255)
                                                                                                                                              aGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAAc   >  2:2767676/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACC  >  CP009273/1855093‑1855383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A